8KG8: Yeast replisome in state II
Yeast replisome in state II. Determined by electron microscopy at 4.23 Å resolution. Released 6 Dec 2023.
- Method
- Electron microscopy
- Resolution
- 4.23 Å
- Organisms
- Saccharomyces cerevisiae S288C, Saccharomyces cerevisiae
- Chains
- 18
- Atoms
- 63,829
- Mol. weight
- 1477.7 kDa
- Ligands
- AGS, MG, ADP, ZN
- Released
- 6 Dec 2023
Explore 8KG8 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8KG8 contains 348 α-helices and 368 β-strands across 16 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain 2: 31 helices, 35 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 183-187 | 5 | |
| α-helix | 194-198 | 5 | |
| α-helix | 201-217 | 17 | |
| β-strand | 219 | 1 | 1 |
| β-strand | 225 | 1 | 1 |
| α-helix | 226-237 | 12 | |
| β-strand | 241-242 | 2 | 2 |
| β-strand | 245 | 1 | 3 |
| α-helix | 246-251 | 6 | |
| α-helix | 254-262 | 9 | |
| α-helix | 264-282 | 19 | |
| α-helix | 286-288 | 3 | |
| β-strand | 294-295 | 2 | 2 |
| β-strand | 298 | 1 | 3 |
| β-strand | 303-304 | 2 | 4 |
| α-helix | 311-313 | 3 | |
| β-strand | 317-318 | 2 | 5 |
| β-strand | 319-320 | 2 | 4 |
| β-strand | 321-327 | 7 | 5 |
| β-strand | 331-334 | 4 | 6 |
| β-strand | 336-337 | 2 | 7 |
| β-strand | 339-341 | 3 | 8 |
| β-strand | 351-352 | 2 | 7 |
| β-strand | 373-375 | 3 | 8 |
| β-strand | 382-385 | 4 | 6 |
| β-strand | 386-391 | 6 | 5 |
| α-helix | 398 | 1 | |
| α-helix | 402-404 | 3 | |
| β-strand | 405-410 | 6 | 5 |
| β-strand | 423-433 | 11 | 5 |
| α-helix | 436-442 | 7 | |
| β-strand | 446 | 1 | 9 |
| β-strand | 448-458 | 11 | 5 |
| α-helix | 478-488 | 11 | |
| α-helix | 493-499 | 7 | |
| α-helix | 509-520 | 12 | |
| β-strand | 526-527 | 2 | 10 |
| β-strand | 531-532 | 2 | 10 |
| β-strand | 539-543 | 5 | 11 |
| α-helix | 549-559 | 11 | |
| β-strand | 563-567 | 5 | 11 |
| β-strand | 571 | 1 | 12 |
| α-helix | 573-577 | 5 | |
| β-strand | 578-582 | 5 | 13 |
| β-strand | 589-593 | 5 | 13 |
| α-helix | 595-598 | 4 | |
| β-strand | 603-607 | 5 | 11 |
| α-helix | 614-617 | 4 | |
| α-helix | 618-620 | 3 | |
| α-helix | 621-625 | 5 | |
| β-strand | 628-633 | 6 | 14 |
| β-strand | 636-641 | 6 | 14 |
| β-strand | 646-650 | 5 | 11 |
| α-helix | 653-655 | 3 | |
| α-helix | 663-666 | 4 | |
| α-helix | 671-676 | 6 | |
| β-strand | 679-683 | 5 | 11 |
| α-helix | 689-705 | 17 | |
| α-helix | 740-755 | 16 | |
| α-helix | 760-773 | 14 | |
| β-strand | 777 | 1 | 15 |
| α-helix | 783-798 | 16 | |
| α-helix | 807-823 | 17 | |
| β-strand | 828 | 1 | 15 |
| α-helix | 831-847 | 17 | |
| α-helix | 851-861 | 11 | |
Chain 3: 27 helices, 29 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 19-34 | 16 | |
| α-helix | 36-54 | 19 | |
| β-strand | 95-99 | 5 | 16 |
| α-helix | 100-106 | 7 | |
| α-helix | 108-116 | 9 | |
| α-helix | 118-120 | 3 | |
| α-helix | 122-135 | 14 | |
| β-strand | 154-158 | 5 | 16 |
| β-strand | 159 | 1 | 17 |
| α-helix | 162-164 | 3 | |
| β-strand | 165-166 | 2 | 18 |
| α-helix | 173-175 | 3 | |
| β-strand | 179-189 | 11 | 18 |
| α-helix | 190-192 | 3 | |
| β-strand | 193-203 | 11 | 18 |
| β-strand | 208-213 | 6 | 18 |
| α-helix | 225-227 | 3 | |
| β-strand | 234 | 1 | 19 |
| β-strand | 240 | 1 | 19 |
| β-strand | 241-243 | 3 | 18 |
| β-strand | 249-259 | 11 | 18 |
| α-helix | 266 | 1 | |
| α-helix | 270-272 | 3 | |
| β-strand | 273-279 | 7 | 18 |
| α-helix | 280-282 | 3 | |
| β-strand | 291-301 | 11 | 18 |
| β-strand | 318-327 | 10 | 18 |
| β-strand | 329-330 | 2 | 20 |
| β-strand | 336-337 | 2 | 20 |
| α-helix | 344-354 | 11 | |
| α-helix | 359-367 | 9 | |
| α-helix | 375-385 | 11 | |
| β-strand | 391 | 1 | 21 |
| β-strand | 399 | 1 | 21 |
| β-strand | 405-409 | 5 | 22 |
| α-helix | 415-425 | 11 | |
| β-strand | 429-433 | 5 | 22 |
| β-strand | 437 | 1 | 23 |
| α-helix | 439-442 | 4 | |
| β-strand | 444-448 | 5 | 24 |
| β-strand | 455-459 | 5 | 24 |
| β-strand | 469-473 | 5 | 22 |
| α-helix | 475-477 | 3 | |
| α-helix | 480-492 | 13 | |
| β-strand | 494-499 | 6 | 25 |
| β-strand | 502-507 | 6 | 25 |
| β-strand | 511-516 | 6 | 22 |
| α-helix | 537-541 | 5 | |
| β-strand | 545-549 | 5 | 22 |
| α-helix | 556-569 | 14 | |
| β-strand | 592 | 1 | 17 |
| α-helix | 653-666 | 14 | |
| α-helix | 673-687 | 15 | |
| α-helix | 691-693 | 3 | |
| α-helix | 699-715 | 17 | |
| α-helix | 723-737 | 15 | |
Chain 4: 28 helices, 35 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 187-198 | 12 | |
| α-helix | 203-209 | 7 | |
| α-helix | 225-236 | 12 | |
| β-strand | 244 | 1 | 26 |
| α-helix | 246-250 | 5 | |
| α-helix | 255-263 | 9 | |
| α-helix | 265-286 | 22 | |
| α-helix | 293-298 | 6 | |
| β-strand | 306 | 1 | 26 |
| α-helix | 314-316 | 3 | |
| α-helix | 319-321 | 3 | |
| β-strand | 324-335 | 12 | 27 |
| β-strand | 339-344 | 6 | 28 |
| β-strand | 347-349 | 3 | 29 |
| β-strand | 355-356 | 2 | 29 |
| β-strand | 359 | 1 | 28 |
| β-strand | 364 | 1 | 30 |
| β-strand | 370 | 1 | 31 |
| β-strand | 379 | 1 | 31 |
| β-strand | 382-384 | 3 | 29 |
| α-helix | 386-388 | 3 | |
| β-strand | 390-394 | 5 | 28 |
| β-strand | 397-401 | 5 | 27 |
| α-helix | 411-412 | 2 | |
| β-strand | 414-418 | 5 | 27 |
| β-strand | 420 | 1 | 28 |
| β-strand | 432-441 | 10 | 27 |
| β-strand | 444-445 | 2 | 32 |
| β-strand | 453-454 | 2 | 32 |
| β-strand | 459-467 | 9 | 27 |
| α-helix | 503-514 | 12 | |
| α-helix | 518-526 | 9 | |
| α-helix | 534-545 | 12 | |
| β-strand | 563-567 | 5 | 33 |
| α-helix | 572-584 | 13 | |
| β-strand | 590-592 | 3 | 33 |
| β-strand | 604-607 | 4 | 34 |
| α-helix | 610-612 | 3 | |
| β-strand | 614-617 | 4 | 34 |
| β-strand | 625 | 1 | 35 |
| β-strand | 628-632 | 5 | 33 |
| α-helix | 639-650 | 12 | |
| β-strand | 653-658 | 6 | 36 |
| β-strand | 661-666 | 6 | 36 |
| β-strand | 669 | 1 | 35 |
| β-strand | 670-674 | 5 | 33 |
| α-helix | 688-692 | 5 | |
| α-helix | 696-700 | 5 | |
| β-strand | 704-707 | 4 | 33 |
| α-helix | 714-730 | 17 | |
| α-helix | 747-757 | 11 | |
| β-strand | 762 | 1 | 37 |
| α-helix | 764-781 | 18 | |
| α-helix | 795-810 | 16 | |
| α-helix | 811-813 | 3 | |
| β-strand | 817 | 1 | 37 |
| α-helix | 819-836 | 18 | |
| α-helix | 852-873 | 22 | |
| β-strand | 881 | 1 | 38 |
| α-helix | 883-888 | 6 | |
| α-helix | 901-912 | 12 | |
| β-strand | 916-918 | 3 | 38 |
| β-strand | 924-926 | 3 | 38 |
Chain 5: 36 helices, 29 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 21-37 | 17 | |
| α-helix | 45-55 | 11 | |
| β-strand | 60-64 | 5 | 39 |
| α-helix | 65-71 | 7 | |
| α-helix | 73-81 | 9 | |
| α-helix | 83-102 | 20 | |
| α-helix | 132-135 | 4 | |
| β-strand | 136-140 | 5 | 39 |
| α-helix | 148-150 | 3 | |
| β-strand | 159-169 | 11 | 40 |
| α-helix | 170-172 | 3 | |
| β-strand | 173-183 | 11 | 41 |
| β-strand | 190-193 | 4 | 41 |
| β-strand | 241-243 | 3 | 41 |
| β-strand | 249-253 | 5 | 41 |
| β-strand | 255-259 | 5 | 40 |
| α-helix | 262-264 | 3 | |
| α-helix | 266 | 1 | |
| α-helix | 270-272 | 3 | |
| β-strand | 273-277 | 5 | 40 |
| α-helix | 280-282 | 3 | |
| β-strand | 291-301 | 11 | 40 |
| β-strand | 323 | 1 | 6 |
| β-strand | 325-333 | 9 | 40 |
| α-helix | 340-343 | 4 | |
| α-helix | 351-361 | 11 | |
| α-helix | 366-372 | 7 | |
| α-helix | 382-393 | 12 | |
| β-strand | 398-399 | 2 | 42 |
| β-strand | 405-406 | 2 | 42 |
| β-strand | 411-416 | 6 | 43 |
| α-helix | 422-432 | 11 | |
| β-strand | 436-439 | 4 | 43 |
| β-strand | 444 | 1 | 14 |
| α-helix | 446-449 | 4 | |
| β-strand | 451-455 | 5 | 44 |
| β-strand | 462-466 | 5 | 44 |
| α-helix | 468-471 | 4 | |
| β-strand | 476-479 | 4 | 43 |
| α-helix | 482-484 | 3 | |
| α-helix | 487-490 | 4 | |
| α-helix | 491-493 | 3 | |
| α-helix | 494-499 | 6 | |
| β-strand | 501-506 | 6 | 23 |
| β-strand | 509-514 | 6 | 23 |
| β-strand | 518-522 | 5 | 43 |
| α-helix | 536-539 | 4 | |
| α-helix | 544-549 | 6 | |
| β-strand | 552-556 | 5 | 43 |
| α-helix | 562-576 | 15 | |
| α-helix | 579-590 | 12 | |
| α-helix | 596-609 | 14 | |
| β-strand | 613-614 | 2 | 45 |
| α-helix | 616-640 | 25 | |
| α-helix | 650-666 | 17 | |
| β-strand | 671-672 | 2 | 45 |
| α-helix | 674-684 | 11 | |
| α-helix | 685-689 | 5 | |
| α-helix | 690-692 | 3 | |
| α-helix | 708-721 | 14 | |
| β-strand | 727-729 | 3 | 46 |
| α-helix | 730-736 | 7 | |
| α-helix | 750-757 | 8 | |
| β-strand | 761-763 | 3 | 46 |
| β-strand | 769-772 | 4 | 46 |
Chain 6: 28 helices, 26 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 106-120 | 15 | |
| α-helix | 135-146 | 12 | |
| β-strand | 150-154 | 5 | 47 |
| α-helix | 155-160 | 6 | |
| α-helix | 161-164 | 4 | |
| α-helix | 165-172 | 8 | |
| α-helix | 174-192 | 19 | |
| β-strand | 264-268 | 5 | 47 |
| α-helix | 281-283 | 3 | |
| β-strand | 287-288 | 2 | 9 |
| β-strand | 291-297 | 7 | 9 |
| β-strand | 301-311 | 11 | 9 |
| α-helix | 316 | 1 | |
| β-strand | 317-322 | 6 | 9 |
| β-strand | 344-346 | 3 | 9 |
| β-strand | 352-362 | 11 | 9 |
| α-helix | 365-367 | 3 | |
| α-helix | 368-369 | 2 | |
| α-helix | 372-375 | 4 | |
| β-strand | 376-382 | 7 | 9 |
| α-helix | 383-385 | 3 | |
| β-strand | 394-405 | 12 | 9 |
| α-helix | 407-410 | 4 | |
| β-strand | 437-438 | 2 | 30 |
| β-strand | 447-448 | 2 | 30 |
| β-strand | 450-461 | 12 | 9 |
| α-helix | 501-506 | 6 | |
| α-helix | 510-521 | 12 | |
| α-helix | 525-532 | 8 | |
| α-helix | 541-552 | 12 | |
| β-strand | 558 | 1 | 48 |
| β-strand | 564 | 1 | 48 |
| β-strand | 571-575 | 5 | 49 |
| α-helix | 581-591 | 11 | |
| β-strand | 596-599 | 4 | 49 |
| β-strand | 610-614 | 5 | 50 |
| β-strand | 621-625 | 5 | 50 |
| α-helix | 627-630 | 4 | |
| β-strand | 635-639 | 5 | 49 |
| α-helix | 646-649 | 4 | |
| α-helix | 650-652 | 3 | |
| β-strand | 660-664 | 5 | 12 |
| β-strand | 669-673 | 5 | 12 |
| β-strand | 677-681 | 5 | 49 |
| α-helix | 683-684 | 2 | |
| α-helix | 703-706 | 4 | |
| β-strand | 711-715 | 5 | 49 |
| α-helix | 721-736 | 16 | |
| α-helix | 748-759 | 12 | |
| β-strand | 764-765 | 2 | 51 |
| α-helix | 767-783 | 17 | |
| α-helix | 797-813 | 17 | |
| β-strand | 818-819 | 2 | 51 |
| α-helix | 821-835 | 15 | |
Chain 7: 31 helices, 21 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 7-8 | 2 | 18 |
| α-helix | 14-26 | 13 | |
| α-helix | 62-71 | 10 | |
| β-strand | 78-81 | 4 | 52 |
| α-helix | 82-94 | 13 | |
| α-helix | 102-106 | 5 | |
| α-helix | 110-123 | 14 | |
| α-helix | 125-128 | 4 | |
| α-helix | 138-154 | 17 | |
| β-strand | 202-205 | 4 | 52 |
| α-helix | 206-207 | 2 | |
| β-strand | 225 | 1 | 53 |
| α-helix | 232-234 | 3 | |
| β-strand | 238-248 | 11 | 53 |
| β-strand | 252-262 | 11 | 53 |
| β-strand | 268-272 | 5 | 53 |
| β-strand | 277-278 | 2 | 32 |
| α-helix | 287-292 | 6 | |
| α-helix | 296-297 | 2 | |
| β-strand | 298-299 | 2 | 53 |
| β-strand | 306-317 | 12 | 53 |
| α-helix | 318 | 1 | |
| α-helix | 319-321 | 3 | |
| β-strand | 330-336 | 7 | 53 |
| α-helix | 337-339 | 3 | |
| β-strand | 348-356 | 9 | 53 |
| α-helix | 364-368 | 5 | |
| β-strand | 371 | 1 | 18 |
| β-strand | 375-383 | 9 | 53 |
| α-helix | 396-407 | 12 | |
| α-helix | 410-418 | 9 | |
| α-helix | 426-437 | 12 | |
| β-strand | 456-458 | 3 | 54 |
| α-helix | 466-476 | 11 | |
| β-strand | 481-484 | 4 | 54 |
| α-helix | 514-516 | 3 | |
| β-strand | 520-524 | 5 | 54 |
| α-helix | 531-541 | 11 | |
| β-strand | 545-547 | 3 | 55 |
| β-strand | 556-558 | 3 | 55 |
| α-helix | 561 | 1 | |
| β-strand | 562-566 | 5 | 54 |
| α-helix | 580-583 | 4 | |
| α-helix | 588-593 | 6 | |
| β-strand | 596-598 | 3 | 54 |
| α-helix | 608-622 | 15 | |
| α-helix | 636-648 | 13 | |
| α-helix | 651-653 | 3 | |
| α-helix | 654-674 | 21 | |
| α-helix | 680-682 | 3 | |
| α-helix | 686-702 | 17 | |
| α-helix | 710-727 | 18 | |
Chain A: 7 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-21 | 18 | |
| α-helix | 27-32 | 6 | |
| α-helix | 34-60 | 27 | |
| α-helix | 65-104 | 40 | |
| α-helix | 120-122 | 3 | |
| α-helix | 125-144 | 20 | |
| β-strand | 162-166 | 5 | 56 |
| β-strand | 167 | 1 | 57 |
| β-strand | 172-174 | 3 | 58 |
| β-strand | 181-183 | 3 | 58 |
| β-strand | 188-192 | 5 | 56 |
| α-helix | 197-202 | 6 | |
| β-strand | 205 | 1 | 57 |
Chain B: 9 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-7 | 3 | |
| α-helix | 13-20 | 8 | |
| β-strand | 24-27 | 4 | 59 |
| α-helix | 58-61 | 4 | |
| β-strand | 69-72 | 4 | 59 |
| α-helix | 73-80 | 8 | |
| β-strand | 87 | 1 | 59 |
| α-helix | 91-93 | 3 | |
| α-helix | 95-107 | 13 | |
| α-helix | 119-129 | 11 | |
| α-helix | 138-160 | 23 | |
| β-strand | 168-169 | 2 | 60 |
| α-helix | 175-199 | 25 | |
8 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| DNA replication licensing factor MCM2 | 2 | protein | 868 | Saccharomyces cerevisiae S288C | P29469 (AlphaFold model) |
| DNA replication licensing factor MCM3 | 3 | protein | 971 | Saccharomyces cerevisiae S288C | P24279 (AlphaFold model) |
| DNA replication licensing factor MCM4 | 4 | protein | 933 | Saccharomyces cerevisiae S288C | P30665 (AlphaFold model) |
| Minichromosome maintenance protein 5 | 5 | protein | 775 | Saccharomyces cerevisiae S288C | P29496 (AlphaFold model) |
| DNA replication licensing factor MCM6 | 6 | protein | 1017 | Saccharomyces cerevisiae S288C | P53091 |
| DNA replication licensing factor MCM7 | 7 | protein | 845 | Saccharomyces cerevisiae S288C | P38132 |
| DNA replication complex GINS protein PSF1 | A | protein | 208 | Saccharomyces cerevisiae S288C | Q12488 |
| DNA replication complex GINS protein PSF2 | B | protein | 213 | Saccharomyces cerevisiae S288C | P40359 |
| DNA replication complex GINS protein PSF3 | C | protein | 194 | Saccharomyces cerevisiae S288C | Q12146 |
| DNA replication complex GINS protein SLD5 | D | protein | 294 | Saccharomyces cerevisiae S288C | Q03406 |
| Cell division control protein 45 | E | protein | 650 | Saccharomyces cerevisiae S288C | Q08032 |
| DNA polymerase alpha-binding protein | F, G, H | protein | 927 | Saccharomyces cerevisiae S288C | Q01454 |
4 more molecules are not listed.
Sequence of entity 1 (2), FASTA
>8KG8_1 DNA replication licensing factor MCM2 (chains 2)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH
Sequence of entity 2 (3), FASTA
>8KG8_2 DNA replication licensing factor MCM3 (chains 3)
MEGSTGFDGDATTFFAPDAVFGDRVRRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQ
DDADERDLLGDDDGDDLEKEKKAASSTSLNILPHRIIISLDDLREFDRSFWSGILVEPAY
FIPPAEKALTDLADSMDDVPHPNASAVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLV
SVEGIVTKTSLVRPKLIRSVHYAAKTGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNK
LTTEYGYSTFIDHQRITVQEMPEMAPAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKS
LGAGGMNQSNSNTLIGFKTLILGNTVYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIF
DILSQSLAPSIYGHDHIKKAILLMLMGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLR
FVLNTASLAIATTGRGSSGVGLTAAVTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTD
VDRVAIHEVMEQQTVTIAKAGIHTTLNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLL
SRFDLLFVVTDDINEIRDRSISEHVLRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINP
EEHSNSGAGVENEGEDDEDHVFEKFNPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYV
QYAKERVIPQLTQEAINVIVKNYTDLRNDDNTKKSPITARTLETLIRLATAHAKVRLSKT
VNKVDAKVAANLLRFALLGEDIGNDIDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSG
SPIKSTPRRSTASSVNATPSSARRILRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQL
GLRVSPRRREHLHAPEEGSSGPLTEVGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTIST
GRLSLISGIIARLMQTEIFEEESYPVASLFERINEELPEEEKFSAQEYLAGLKIMSDRNN
LMVADDKVWRV
Sequence of entity 3 (4), FASTA
>8KG8_3 DNA replication licensing factor MCM4 (chains 4)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV
Sequence of entity 4 (5), FASTA
>8KG8_4 Minichromosome maintenance protein 5 (chains 5)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV
Sequence of entity 5 (6), FASTA
>8KG8_5 DNA replication licensing factor MCM6 (chains 6)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS
Sequence of entity 6 (7), FASTA
>8KG8_6 DNA replication licensing factor MCM7 (chains 7)
MSAALPSIQLPVDYNNLFNEITDFLVTFKQDTLSSDATRNENEDENLDAENIEQHLLEKG
PKYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRA
IDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDA
LREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLIT
VRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFM
STRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPY
TGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAP
EIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRG
VYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEV
MEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLM
LDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYV
VQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLV
RVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLS
NCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANVSAQDSDI
DLQDA
Sequence of entity 7 (A), FASTA
>8KG8_7 DNA replication complex GINS protein PSF1 (chains A)
MYGDLGNKLVLEAKRTKQLYARSNQDVNLPMYHEDIIRNILKEVSNLRKNTEYLKEQQQL
GMLDDKVAKCQYFVTLLCMERNKRCLLAYQRLRTDILDSMAWNNNGLDLMSSITFSQQDT
NNLSHQEQEYLKEYCDLITDLKSGDLVDIDLSGSLVPPSDVFIDVRVLKDAGEIQTEYGV
FNLIKDSQFFVRQSDVERLIQQGYLQKI
Sequence of entity 8 (B), FASTA
>8KG8_8 DNA replication complex GINS protein PSF2 (chains B)
MSLPAHLQQTFSPEEIQFIVENEPIKIFPRITTRQKIRGDDRGTGNHTRWQLITTDDKAL
NNMVAMRSTEVVLWIALLLKQQSKCSIVAPQWLTTKELDRKIQYEKTHPDRFSELPWNWL
VLARILFNKAKDDFHDPIHELRGKIQDLREIRQIKVLKGLKYLNESHLQLDNLSLLEINE
LRPFITEIMDKLREIHTASLTAGTENDEEEFNI
Sequence of entity 9 (C), FASTA
>8KG8_9 DNA replication complex GINS protein PSF3 (chains C)
MGYYDIDDVLADGTEFPCKFQYDIPGLGYLENNPGRPITKNTKLSLPLWLARILAIVGGD
EALVDEEPVPFVELLPPDMFSTKVMNAIKTDPVALDLHSINSHFFSLAIKWIMLFSEKEL
ANVVSELLLQRAQELNHHASSLSIDLNADSTGKNSANTNIATSTFLLKLEEMEKEIYKKS
HESYKDTKRWMFKK
Sequence of entity 10 (D), FASTA
>8KG8_10 DNA replication complex GINS protein SLD5 (chains D)
MDINIDDILAELDKETTAVDSTKITQGSSSTTHRDANTIVGSSLDLNDKTQIYVSPQQDF
SDLMKSWKNERCSPELLPYPHQLMKRLLNRISMQSQLIENISMGFLDMQNASNANPPMPN
ESKLPLLCMETELERLKFVIRSYIRCRLSKIDKFSLYLRQLNEDENSLISLTDLLSKDEI
KYHDTHSLIWLKLVNDSILKYMPEELQAINDTEGSVNMIDEPDWNKFVFIHVNGPPDGKW
NEDPLLQENEFGKPCYTVTIPDLKEEVELTIGSIYVMRYEVIRDLLRDDKVALI
Sequence of entity 11 (E), FASTA
>8KG8_11 Cell division control protein 45 (chains E)
MYYGISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFKKQLVQSQIVP
IFGYSELRRHYSQLDDNINSLLLVGFGGVIDLEAFLEIDPQEYVIDTDEKSGEQSFRRDI
YVLDAHRPWNLDNIFGSQIIQCFDDGTVDDTLGEQKEAYYKLLELDEESGDDELSGDEND
NNGGDDEATDADEVTDEDEEDEDETISNKRGNSSIGPNDLSKRKQRKKQIHEYEGVLEEY
YSQGTTVVNSISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYPLLQDEVK
RLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSLWNENGKKRLH
KMFARMGIPLSTAQETWLYMDHSIKRELGIIFDKNLDRYGLQDIIRDGFVRTLGYRGSIS
ASEFVEALTALLEVGNSTDKDSVKINNDNNDDTDGEEEEDNSAQKLTNLRKRWVSNFWLS
WDALDDRKVELLNRGIQLAQDLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDGPDLDLYR
NPLTLLRLGNWLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGLDTIHTKKP
ILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL
Sequence of entity 12 (F, G, H), FASTA
>8KG8_12 DNA polymerase alpha-binding protein (chains F, G, H)
MVSVIDKLVFDFGGKTLVSLAPDNNTLCVANKNGLTKILKTNNPEEEPETLDSSKLVSSI
KCYSNSHFLMTTMQGDALRYNIDSSQEELLARFALPLRDCCVIHSGKMAVFGGDDLELIL
LELDDETHKKHAIKIDEQVSQISYNSQMNILAVSMINGKVQIFSLTSTIPNKVHELNDYI
VANSYDDTHRDKILSNMMDDIDKDNDNDLSETADPDENNVADPEFCAANRICTRVAWHPK
GLHFALPCADDTVKIFSIKGYSLQKTLSTNLSSTKAHFIDLQFDPLRGTYIAAVDLNNKL
TVWNWETSEIHYTREFKRKITNIAWKIQADSKTLDLVLGTWSGSIAIVQNLAESVVSNIP
DQSVAESSTKHGLFVDSESDLENLEGNDDINKSDKLFSDITQEANAEDVFTQTHDGPSGL
SEKRKYNFEDEEDFIDDDDGAGYISGKKPHNEHSYSRVHKTHSFPISLANTGKFRYMPFS
PAGTPFGFTDRRYLTMNEVGYVSTVKNSEQYSITVSFFDVGRFREYHFEDLFGYDLCFLN
EKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSF
NQFGVPFAVEKTSPIVALTAQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMS
LPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPEESKWLP
ILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPV
FVKSKLLEENKAILNKKNEIGADTEAEEGEEDKEIQIPVSMAAEEEYLRSKVLSELLTDT
LENDGEMYGNENEVLAALNGAYDKALLRLFASACSDQNVEKALSLAHELKQDRALTAAVK
ISERAELPSLVKKINNIREARYEQQLK
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| AGS | Phosphothiophosphoric acid-adenylate ester | C10 H16 N5 O12 P3 S | 3 |
| MG | Magnesium ion | Mg | 5 |
| ADP | Adenosine-5'-diphosphate | C10 H15 N5 O10 P2 | 2 |
| ZN | Zinc ion | Zn | 7 |
Primary citation
Synergism between CMG helicase and leading strand DNA polymerase at replication fork. Xu, Z., Feng, J., Yu, D. et al. Nat Commun (2023) 14:5849-5849. DOI 10.1038/s41467-023-41506-0 · PubMed
Other PDB entries of the same protein (UniProt P29469 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7W8G 2.52 Å, Cryo-EM structure of MCM double hexamer
- 28VY 2.7 Å, sCMGE assembled on ARS1 DNA with Sld2 and RPA
- 8RIF 2.79 Å, Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
- 7V3V 2.9 Å, Cryo-EM structure of MCM double hexamer bound with DDK in State I
- 9TH8 2.9 Å, Cryo-EM structure of MCM2-7 DH bound to Sld3-Sld7, Cdc45 and DNA
- 7P30 3.0 Å, 3.0 A resolution structure of a DNA-loaded MCM double hexamer
- 8KG6 3.07 Å, Yeast replisome in state I
- 9RHL 3.1 Å, Phospho-DH bound by Sld3-MBD on ARS1 DNA
- 7PMK 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
- 7PMN 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
- 7PT6 3.2 Å, Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III
- 7V3U 3.2 Å, Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD
Browse structure collections
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