8KG9: Yeast replisome in state III

Name: PDB 8KG9: Yeast replisome in state III
Published: 2023-12-06
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: replication

Yeast replisome in state III. Determined by electron microscopy at 4.52 Å resolution. Released 6 Dec 2023.

Method: Electron microscopy
Resolution: 4.52 Å
Organisms: Saccharomyces cerevisiae, Saccharomyces cerevisiae S288C
Chains: 18
Atoms: 63,507
Mol. weight: 1477.15 kDa
Ligands: ZN, ADP, MG, AGS
Released: 6 Dec 2023

Explore 8KG9 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8KG9 contains 339 α-helices and 357 β-strands across 16 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 2: 32 helices, 32 β-strands

Element	Residues	Length	Sheet
α-helix	183-187	5
α-helix	194-199	6
α-helix	201-212	12
α-helix	213-217	5
α-helix	226-230	5
α-helix	233-237	5
β-strand	243	1	1
α-helix	255-262	8
α-helix	264-282	19
β-strand	296	1	1
α-helix	306-308	3
α-helix	311-313	3
β-strand	317-319	3	2
β-strand	322	1	3
α-helix	328-330	3
β-strand	333-334	2	4
β-strand	336-337	2	5
β-strand	339-341	3	6
β-strand	351-352	2	5
β-strand	373-375	3	6
β-strand	382-383	2	4
β-strand	385-389	5	2
α-helix	398	1
α-helix	402-404	3
β-strand	407-411	5	2
β-strand	424	1	3
β-strand	427-433	7	2
α-helix	436-442	7
β-strand	448-455	8	2
β-strand	458	1	3
α-helix	478-489	12
α-helix	493-500	8
α-helix	509-520	12
β-strand	525-527	3	7
β-strand	531-533	3	7
β-strand	539-542	4	8
α-helix	549-556	8
β-strand	563-566	4	8
α-helix	573-577	5
β-strand	579-583	5	9
β-strand	588-592	5	9
α-helix	595-599	5
β-strand	603-606	4	8
α-helix	609-611	3
α-helix	614-625	12
β-strand	628-630	3	10
β-strand	632	1	11
β-strand	633	1	12
β-strand	636	1	12
β-strand	639-641	3	10
β-strand	645-650	6	8
α-helix	653-655	3
α-helix	663-666	4
α-helix	671-676	6
β-strand	679-682	4	8
α-helix	689-706	18
α-helix	740-754	15
α-helix	760-773	14
β-strand	777	1	13
α-helix	783-793	11
α-helix	807-824	18
β-strand	828	1	13
α-helix	831-848	18
α-helix	851-865	15

Chain 3: 27 helices, 27 β-strands

Element	Residues	Length	Sheet
α-helix	19-34	16
α-helix	36-54	19
β-strand	95-97	3	14
α-helix	108-116	9
α-helix	118-137	20
α-helix	144-149	6
β-strand	154-156	3	14
β-strand	179-189	11	15
α-helix	190-192	3
β-strand	193	1	16
β-strand	196	1	17
β-strand	198-201	4	18
β-strand	202	1	19
β-strand	210-213	4	18
α-helix	224-226	3
β-strand	242	1	19
β-strand	250	1	17
β-strand	253	1	16
β-strand	255-259	5	15
α-helix	266	1
α-helix	270-272	3
β-strand	273-277	5	15
β-strand	291-301	11	15
α-helix	302-305	4
α-helix	315-317	3
β-strand	318-327	10	15
α-helix	344-348	5
α-helix	351-355	5
α-helix	359-366	8
α-helix	375-385	11
α-helix	387-389	3
β-strand	404-409	6	20
α-helix	415-425	11
β-strand	430	1	20
β-strand	433	1	20
α-helix	439-443	5
β-strand	445	1	21
β-strand	448	1	22
β-strand	455	1	22
β-strand	458	1	21
α-helix	462-465	4
β-strand	469-473	5	20
α-helix	480-492	13
β-strand	494-495	2	23
β-strand	506-507	2	23
β-strand	511-516	6	20
α-helix	529-532	4
β-strand	545-549	5	20
α-helix	555-570	16
α-helix	572-573	2
α-helix	653-666	14
α-helix	673-687	15
α-helix	698-716	19
α-helix	723-738	16

Chain 4: 29 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	187-194	8
α-helix	218-222	5
α-helix	225-234	10
β-strand	240-241	2	24
α-helix	245-250	6
α-helix	252-263	12
α-helix	265-286	22
α-helix	293-298	6
β-strand	302-303	2	24
β-strand	311	1	25
β-strand	325-335	11	25
α-helix	336-338	3
β-strand	339-342	4	25
β-strand	344-349	6	26
β-strand	355-359	5	26
β-strand	360	1	27
β-strand	382-383	2	26
β-strand	391-401	11	25
α-helix	402	1
α-helix	411-412	2
β-strand	414-420	7	25
β-strand	432-441	10	25
β-strand	444-445	2	28
β-strand	453-454	2	28
β-strand	459-468	10	25
α-helix	506-514	9
α-helix	518-525	8
α-helix	534-545	12
α-helix	561-562	2
β-strand	563	1	29
α-helix	572-582	11
β-strand	591-592	2	29
β-strand	604-606	3	30
β-strand	615-617	3	30
β-strand	628-632	5	29
α-helix	638-651	14
β-strand	654-658	5	31
β-strand	661-665	5	31
β-strand	670-673	4	29
α-helix	681-682	2
α-helix	688-692	5
α-helix	696-700	5
α-helix	714-730	17
α-helix	745-757	13
β-strand	761-762	2	32
α-helix	764-779	16
α-helix	795-810	16
α-helix	811-813	3
β-strand	816-817	2	32
α-helix	819-834	16
β-strand	847	1	33
β-strand	850	1	33
α-helix	858-875	18
β-strand	882	1	34
α-helix	883-889	7
α-helix	890-894	5
α-helix	900-912	13
β-strand	919	1	34
β-strand	923	1	34

Chain 5: 32 helices, 32 β-strands

Element	Residues	Length	Sheet
α-helix	21-36	16
β-strand	40	1	35
β-strand	43	1	35
α-helix	45-55	11
β-strand	60-64	5	36
α-helix	65-71	7
α-helix	73-81	9
α-helix	83-102	20
α-helix	133-135	3
β-strand	136-140	5	36
β-strand	159-169	11	37
α-helix	170-172	3
β-strand	173-178	6	38
β-strand	179-182	4	39
α-helix	184-186	3
β-strand	189-192	4	39
α-helix	204-208	5
β-strand	242-243	2	39
β-strand	249-253	5	38
β-strand	254-260	7	37
β-strand	273-278	6	37
β-strand	291-301	11	37
β-strand	323	1	4
β-strand	325-335	11	37
α-helix	350-362	13
α-helix	365-373	9
α-helix	382-393	12
β-strand	398-399	2	40
β-strand	405-406	2	40
β-strand	411	1	41
β-strand	412	1	42
β-strand	415-416	2	43
α-helix	422-432	11
β-strand	439-440	2	44
β-strand	444	1	11
β-strand	451-456	6	45
β-strand	459-466	8	45
α-helix	468-472	5
β-strand	475-478	4	42
β-strand	479-480	2	44
α-helix	487-499	13
β-strand	501-503	3	46
α-helix	506-508	3
β-strand	512-514	3	46
β-strand	517-520	4	42
α-helix	521	1
β-strand	523	1	43
α-helix	536-539	4
α-helix	544-550	7
β-strand	551	1	41
β-strand	555-556	2	43
α-helix	562-576	15
α-helix	578-587	10
α-helix	588-593	6
α-helix	596-610	15
α-helix	618-639	22
α-helix	645-649	5
α-helix	651-666	16
α-helix	674-693	20
α-helix	708-719	12
α-helix	731-739	9
α-helix	741-743	3
α-helix	744-748	5
α-helix	749-758	10

Chain 6: 26 helices, 32 β-strands

Element	Residues	Length	Sheet
α-helix	106-119	14
α-helix	139-146	8
β-strand	151-152	2	47
α-helix	155-158	4
α-helix	165-172	8
α-helix	179-191	13
α-helix	260-263	4
β-strand	265-266	2	47
β-strand	286-290	5	48
β-strand	293-297	5	48
β-strand	301	1	49
β-strand	304	1	50
β-strand	309	1	51
β-strand	346	1	51
β-strand	353	1	50
β-strand	356	1	49
β-strand	359-362	4	48
β-strand	376-380	5	48
α-helix	383-388	6
α-helix	390	1
β-strand	393-394	2	48
β-strand	396-402	7	48
α-helix	403-404	2
β-strand	405	1	52
α-helix	406	1
α-helix	416-417	2
β-strand	437	1	27
β-strand	450	1	52
β-strand	453-455	3	48
β-strand	458-460	3	48
α-helix	501-507	7
α-helix	510-521	12
α-helix	525-532	8
α-helix	542-552	11
β-strand	557-558	2	53
β-strand	564-565	2	53
β-strand	570-571	2	54
α-helix	581-591	11
β-strand	596-598	3	54
β-strand	613-614	2	55
β-strand	621-622	2	55
α-helix	624-626	3
α-helix	627-631	5
β-strand	636-638	3	54
α-helix	646-656	11
β-strand	660-661	2	56
β-strand	672-673	2	56
β-strand	678-679	2	54
α-helix	703-708	6
β-strand	711	1	54
α-helix	723-734	12
α-helix	748-760	13
β-strand	764-765	2	57
α-helix	767-783	17
α-helix	788-790	3
α-helix	797-809	13
β-strand	818-819	2	57
α-helix	821-833	13

Chain 7: 34 helices, 30 β-strands

Element	Residues	Length	Sheet
β-strand	7	1	18
α-helix	14-27	14
β-strand	29	1	58
α-helix	30-33	4
β-strand	61	1	58
α-helix	62-72	11
β-strand	80-81	2	59
α-helix	82-95	14
α-helix	99-101	3
α-helix	102-108	7
α-helix	111-122	12
α-helix	126-127	2
α-helix	137-144	8
α-helix	194-197	4
β-strand	204-205	2	59
α-helix	214-216	3
α-helix	232-234	3
β-strand	238-241	4	60
β-strand	244-248	5	61
α-helix	249-251	3
β-strand	252-253	2	61
β-strand	257-262	6	62
β-strand	268-272	5	62
β-strand	277-278	2	28
α-helix	279-280	2
α-helix	287-290	4
α-helix	291-293	3
β-strand	298-300	3	62
β-strand	309-316	8	61
α-helix	317-318	2
α-helix	323	1
β-strand	330-336	7	61
β-strand	348	1	61
β-strand	349	1	63
β-strand	351-354	4	60
β-strand	357-358	2	64
α-helix	359-360	2
α-helix	364-368	5
β-strand	373-374	2	64
β-strand	380	1	60
β-strand	383	1	63
α-helix	398-408	11
α-helix	410-418	9
α-helix	426-437	12
β-strand	456-458	3	65
α-helix	472-476	5
β-strand	480-482	3	66
β-strand	501	1	67
β-strand	507	1	67
α-helix	512-515	4
β-strand	520-522	3	66
α-helix	531-543	13
β-strand	549	1	68
β-strand	553	1	68
β-strand	563-564	2	66
α-helix	570-572	3
α-helix	588-593	6
β-strand	596-598	3	65
α-helix	608-619	12
α-helix	635-643	9
α-helix	650-653	4
α-helix	654-672	19
α-helix	686-702	17
α-helix	710-728	19

Chain A: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	2-22	21
α-helix	28-32	5
α-helix	34-60	27
α-helix	68-100	33
α-helix	101-106	6
α-helix	125-142	18
α-helix	157-158	2
β-strand	163-164	2	69
β-strand	166-167	2	70
β-strand	172-173	2	71
β-strand	182-183	2	71
β-strand	188-191	4	69
α-helix	193-202	10
β-strand	205-206	2	70

Chain B: 8 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	5-9	5
α-helix	13-21	9
β-strand	24-29	6	72
α-helix	57-60	4
β-strand	69-72	4	72
α-helix	73-81	9
β-strand	85-87	3	72
α-helix	95-107	13
α-helix	119-129	11
α-helix	138-161	24
β-strand	168-170	3	73
α-helix	175-199	25

8 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA replication licensing factor MCM2	2	protein	868	Saccharomyces cerevisiae	P29469 (AlphaFold model)
DNA replication licensing factor MCM3	3	protein	971	Saccharomyces cerevisiae S288C	P24279 (AlphaFold model)
DNA replication licensing factor MCM4	4	protein	933	Saccharomyces cerevisiae S288C	P30665 (AlphaFold model)
Minichromosome maintenance protein 5	5	protein	775	Saccharomyces cerevisiae S288C	P29496 (AlphaFold model)
DNA replication licensing factor MCM6	6	protein	1017	Saccharomyces cerevisiae S288C	P53091
DNA replication licensing factor MCM7	7	protein	845	Saccharomyces cerevisiae	P38132
DNA replication complex GINS protein PSF1	A	protein	208	Saccharomyces cerevisiae S288C	Q12488
DNA replication complex GINS protein PSF2	B	protein	213	Saccharomyces cerevisiae S288C	P40359
DNA replication complex GINS protein PSF3	C	protein	194	Saccharomyces cerevisiae S288C	Q12146
DNA replication complex GINS protein SLD5	D	protein	294	Saccharomyces cerevisiae S288C	Q03406
Cell division control protein 45	E	protein	650	Saccharomyces cerevisiae S288C	Q08032
DNA polymerase alpha-binding protein	F, G, H	protein	927	Saccharomyces cerevisiae S288C	Q01454

4 more molecules are not listed.

Sequence of entity 1 (2), FASTA

>8KG9_1 DNA replication licensing factor MCM2 (chains 2)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH

Sequence of entity 2 (3), FASTA

>8KG9_2 DNA replication licensing factor MCM3 (chains 3)
MEGSTGFDGDATTFFAPDAVFGDRVRRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQ
DDADERDLLGDDDGDDLEKEKKAASSTSLNILPHRIIISLDDLREFDRSFWSGILVEPAY
FIPPAEKALTDLADSMDDVPHPNASAVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLV
SVEGIVTKTSLVRPKLIRSVHYAAKTGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNK
LTTEYGYSTFIDHQRITVQEMPEMAPAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKS
LGAGGMNQSNSNTLIGFKTLILGNTVYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIF
DILSQSLAPSIYGHDHIKKAILLMLMGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLR
FVLNTASLAIATTGRGSSGVGLTAAVTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTD
VDRVAIHEVMEQQTVTIAKAGIHTTLNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLL
SRFDLLFVVTDDINEIRDRSISEHVLRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINP
EEHSNSGAGVENEGEDDEDHVFEKFNPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYV
QYAKERVIPQLTQEAINVIVKNYTDLRNDDNTKKSPITARTLETLIRLATAHAKVRLSKT
VNKVDAKVAANLLRFALLGEDIGNDIDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSG
SPIKSTPRRSTASSVNATPSSARRILRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQL
GLRVSPRRREHLHAPEEGSSGPLTEVGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTIST
GRLSLISGIIARLMQTEIFEEESYPVASLFERINEELPEEEKFSAQEYLAGLKIMSDRNN
LMVADDKVWRV

Sequence of entity 3 (4), FASTA

>8KG9_3 DNA replication licensing factor MCM4 (chains 4)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV

Sequence of entity 4 (5), FASTA

>8KG9_4 Minichromosome maintenance protein 5 (chains 5)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV

Sequence of entity 5 (6), FASTA

>8KG9_5 DNA replication licensing factor MCM6 (chains 6)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS

Sequence of entity 6 (7), FASTA

>8KG9_6 DNA replication licensing factor MCM7 (chains 7)
MSAALPSIQLPVDYNNLFNEITDFLVTFKQDTLSSDATRNENEDENLDAENIEQHLLEKG
PKYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRA
IDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDA
LREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLIT
VRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFM
STRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPY
TGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAP
EIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRG
VYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEV
MEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLM
LDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYV
VQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLV
RVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLS
NCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANVSAQDSDI
DLQDA

Sequence of entity 7 (A), FASTA

>8KG9_7 DNA replication complex GINS protein PSF1 (chains A)
MYGDLGNKLVLEAKRTKQLYARSNQDVNLPMYHEDIIRNILKEVSNLRKNTEYLKEQQQL
GMLDDKVAKCQYFVTLLCMERNKRCLLAYQRLRTDILDSMAWNNNGLDLMSSITFSQQDT
NNLSHQEQEYLKEYCDLITDLKSGDLVDIDLSGSLVPPSDVFIDVRVLKDAGEIQTEYGV
FNLIKDSQFFVRQSDVERLIQQGYLQKI

Sequence of entity 8 (B), FASTA

>8KG9_8 DNA replication complex GINS protein PSF2 (chains B)
MSLPAHLQQTFSPEEIQFIVENEPIKIFPRITTRQKIRGDDRGTGNHTRWQLITTDDKAL
NNMVAMRSTEVVLWIALLLKQQSKCSIVAPQWLTTKELDRKIQYEKTHPDRFSELPWNWL
VLARILFNKAKDDFHDPIHELRGKIQDLREIRQIKVLKGLKYLNESHLQLDNLSLLEINE
LRPFITEIMDKLREIHTASLTAGTENDEEEFNI

Sequence of entity 9 (C), FASTA

>8KG9_9 DNA replication complex GINS protein PSF3 (chains C)
MGYYDIDDVLADGTEFPCKFQYDIPGLGYLENNPGRPITKNTKLSLPLWLARILAIVGGD
EALVDEEPVPFVELLPPDMFSTKVMNAIKTDPVALDLHSINSHFFSLAIKWIMLFSEKEL
ANVVSELLLQRAQELNHHASSLSIDLNADSTGKNSANTNIATSTFLLKLEEMEKEIYKKS
HESYKDTKRWMFKK

Sequence of entity 10 (D), FASTA

>8KG9_10 DNA replication complex GINS protein SLD5 (chains D)
MDINIDDILAELDKETTAVDSTKITQGSSSTTHRDANTIVGSSLDLNDKTQIYVSPQQDF
SDLMKSWKNERCSPELLPYPHQLMKRLLNRISMQSQLIENISMGFLDMQNASNANPPMPN
ESKLPLLCMETELERLKFVIRSYIRCRLSKIDKFSLYLRQLNEDENSLISLTDLLSKDEI
KYHDTHSLIWLKLVNDSILKYMPEELQAINDTEGSVNMIDEPDWNKFVFIHVNGPPDGKW
NEDPLLQENEFGKPCYTVTIPDLKEEVELTIGSIYVMRYEVIRDLLRDDKVALI

Sequence of entity 11 (E), FASTA

>8KG9_11 Cell division control protein 45 (chains E)
MYYGISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFKKQLVQSQIVP
IFGYSELRRHYSQLDDNINSLLLVGFGGVIDLEAFLEIDPQEYVIDTDEKSGEQSFRRDI
YVLDAHRPWNLDNIFGSQIIQCFDDGTVDDTLGEQKEAYYKLLELDEESGDDELSGDEND
NNGGDDEATDADEVTDEDEEDEDETISNKRGNSSIGPNDLSKRKQRKKQIHEYEGVLEEY
YSQGTTVVNSISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYPLLQDEVK
RLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSLWNENGKKRLH
KMFARMGIPLSTAQETWLYMDHSIKRELGIIFDKNLDRYGLQDIIRDGFVRTLGYRGSIS
ASEFVEALTALLEVGNSTDKDSVKINNDNNDDTDGEEEEDNSAQKLTNLRKRWVSNFWLS
WDALDDRKVELLNRGIQLAQDLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDGPDLDLYR
NPLTLLRLGNWLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGLDTIHTKKP
ILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL

Sequence of entity 12 (F, G, H), FASTA

>8KG9_12 DNA polymerase alpha-binding protein (chains F, G, H)
MVSVIDKLVFDFGGKTLVSLAPDNNTLCVANKNGLTKILKTNNPEEEPETLDSSKLVSSI
KCYSNSHFLMTTMQGDALRYNIDSSQEELLARFALPLRDCCVIHSGKMAVFGGDDLELIL
LELDDETHKKHAIKIDEQVSQISYNSQMNILAVSMINGKVQIFSLTSTIPNKVHELNDYI
VANSYDDTHRDKILSNMMDDIDKDNDNDLSETADPDENNVADPEFCAANRICTRVAWHPK
GLHFALPCADDTVKIFSIKGYSLQKTLSTNLSSTKAHFIDLQFDPLRGTYIAAVDLNNKL
TVWNWETSEIHYTREFKRKITNIAWKIQADSKTLDLVLGTWSGSIAIVQNLAESVVSNIP
DQSVAESSTKHGLFVDSESDLENLEGNDDINKSDKLFSDITQEANAEDVFTQTHDGPSGL
SEKRKYNFEDEEDFIDDDDGAGYISGKKPHNEHSYSRVHKTHSFPISLANTGKFRYMPFS
PAGTPFGFTDRRYLTMNEVGYVSTVKNSEQYSITVSFFDVGRFREYHFEDLFGYDLCFLN
EKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSF
NQFGVPFAVEKTSPIVALTAQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMS
LPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPEESKWLP
ILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPV
FVKSKLLEENKAILNKKNEIGADTEAEEGEEDKEIQIPVSMAAEEEYLRSKVLSELLTDT
LENDGEMYGNENEVLAALNGAYDKALLRLFASACSDQNVEKALSLAHELKQDRALTAAVK
ISERAELPSLVKKINNIREARYEQQLK

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	7
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	2
MG	Magnesium ion	Mg	4
AGS	Phosphothiophosphoric acid-adenylate ester	C10 H16 N5 O12 P3 S	2

Primary citation

Synergism between CMG helicase and leading strand DNA polymerase at replication fork. Xu, Z., Feng, J., Yu, D. et al. Nat Commun (2023) 14:5849-5849. DOI 10.1038/s41467-023-41506-0 · PubMed

Other PDB entries of the same protein (UniProt P29469 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7W8G 2.52 Å, Cryo-EM structure of MCM double hexamer
28VY 2.7 Å, sCMGE assembled on ARS1 DNA with Sld2 and RPA
8RIF 2.79 Å, Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
7V3V 2.9 Å, Cryo-EM structure of MCM double hexamer bound with DDK in State I
9TH8 2.9 Å, Cryo-EM structure of MCM2-7 DH bound to Sld3-Sld7, Cdc45 and DNA
7P30 3.0 Å, 3.0 A resolution structure of a DNA-loaded MCM double hexamer
8KG6 3.07 Å, Yeast replisome in state I
9RHL 3.1 Å, Phospho-DH bound by Sld3-MBD on ARS1 DNA
7PMK 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
7PMN 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
7PT6 3.2 Å, Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III
7V3U 3.2 Å, Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD

Browse structure collections

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