8P62: DNA replication licensing factor MCM2
S. cerevisiae ssDNA-sCMGE after DNA replication initiation. Determined by electron microscopy at 3.9 Å resolution. Released 29 May 2024.
- Method
- Electron microscopy
- Resolution
- 3.9 Å
- Organisms
- Saccharomyces cerevisiae, synthetic construct
- Chains
- 14
- Atoms
- 53,042
- Mol. weight
- 1135.23 kDa
- Ligands
- ATP, MG, ADP, ZN
- Released
- 29 May 2024
Explore 8P62 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8P62 contains 295 α-helices and 262 β-strands across 13 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain 2: 31 helices, 30 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 183-186 | 4 | |
| α-helix | 194-198 | 5 | |
| α-helix | 201-217 | 17 | |
| α-helix | 226-237 | 12 | |
| β-strand | 241-244 | 4 | 1 |
| α-helix | 246-251 | 6 | |
| α-helix | 254-262 | 9 | |
| α-helix | 265-282 | 18 | |
| α-helix | 286-288 | 3 | |
| β-strand | 294-297 | 4 | 1 |
| β-strand | 304 | 1 | 2 |
| β-strand | 317-327 | 11 | 2 |
| β-strand | 331-334 | 4 | 3 |
| β-strand | 337 | 1 | 4 |
| β-strand | 340-341 | 2 | 5 |
| β-strand | 346-347 | 2 | 5 |
| α-helix | 348-350 | 3 | |
| β-strand | 351 | 1 | 4 |
| β-strand | 373-374 | 2 | 5 |
| β-strand | 382-385 | 4 | 3 |
| β-strand | 386-391 | 6 | 2 |
| α-helix | 398 | 1 | |
| β-strand | 406-410 | 5 | 2 |
| β-strand | 423-433 | 11 | 2 |
| α-helix | 436-442 | 7 | |
| β-strand | 446 | 1 | 6 |
| β-strand | 448-458 | 11 | 2 |
| α-helix | 478-487 | 10 | |
| α-helix | 493-499 | 7 | |
| α-helix | 509-520 | 12 | |
| β-strand | 525-527 | 3 | 7 |
| β-strand | 531-533 | 3 | 7 |
| β-strand | 539-543 | 5 | 8 |
| α-helix | 549-559 | 11 | |
| β-strand | 563-567 | 5 | 8 |
| α-helix | 568-570 | 3 | |
| β-strand | 571 | 1 | 9 |
| α-helix | 573-576 | 4 | |
| β-strand | 579-582 | 4 | 10 |
| β-strand | 589-592 | 4 | 10 |
| α-helix | 595-598 | 4 | |
| β-strand | 602-607 | 6 | 8 |
| α-helix | 621-624 | 4 | |
| β-strand | 628-633 | 6 | 11 |
| β-strand | 636-641 | 6 | 11 |
| β-strand | 644-650 | 7 | 8 |
| α-helix | 653-655 | 3 | |
| α-helix | 663-665 | 3 | |
| α-helix | 671-674 | 4 | |
| β-strand | 679-683 | 5 | 8 |
| α-helix | 689-705 | 17 | |
| α-helix | 740-753 | 14 | |
| α-helix | 760-772 | 13 | |
| β-strand | 777 | 1 | 12 |
| α-helix | 783-794 | 12 | |
| α-helix | 797-799 | 3 | |
| α-helix | 807-823 | 17 | |
| β-strand | 828 | 1 | 12 |
| α-helix | 831-846 | 16 | |
| α-helix | 854-860 | 7 | |
| α-helix | 861-863 | 3 | |
Chain 3: 24 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 20-34 | 15 | |
| α-helix | 37-51 | 15 | |
| β-strand | 96-99 | 4 | 13 |
| α-helix | 100-106 | 7 | |
| α-helix | 108-116 | 9 | |
| α-helix | 118-120 | 3 | |
| α-helix | 122-136 | 15 | |
| α-helix | 146-148 | 3 | |
| β-strand | 155-158 | 4 | 13 |
| α-helix | 162-164 | 3 | |
| β-strand | 165-166 | 2 | 14 |
| α-helix | 173-175 | 3 | |
| β-strand | 178-189 | 12 | 14 |
| β-strand | 195-202 | 8 | 15 |
| β-strand | 209-213 | 5 | 15 |
| β-strand | 234 | 1 | 16 |
| β-strand | 240 | 1 | 16 |
| β-strand | 242-243 | 2 | 15 |
| β-strand | 249-251 | 3 | 15 |
| β-strand | 253-259 | 7 | 14 |
| β-strand | 273-279 | 7 | 14 |
| β-strand | 291-301 | 11 | 14 |
| β-strand | 318-328 | 11 | 14 |
| α-helix | 344-354 | 11 | |
| α-helix | 359-366 | 8 | |
| α-helix | 375-385 | 11 | |
| β-strand | 391-393 | 3 | 17 |
| β-strand | 397-399 | 3 | 17 |
| β-strand | 405-409 | 5 | 18 |
| α-helix | 416-425 | 10 | |
| β-strand | 429-433 | 5 | 18 |
| β-strand | 437 | 1 | 19 |
| α-helix | 439-443 | 5 | |
| β-strand | 444-448 | 5 | 20 |
| β-strand | 455-459 | 5 | 20 |
| α-helix | 461-464 | 4 | |
| β-strand | 469-473 | 5 | 18 |
| α-helix | 475-477 | 3 | |
| α-helix | 480-492 | 13 | |
| β-strand | 494-499 | 6 | 21 |
| β-strand | 502-507 | 6 | 21 |
| β-strand | 511-516 | 6 | 18 |
| α-helix | 529-532 | 4 | |
| α-helix | 537-540 | 4 | |
| β-strand | 545-549 | 5 | 18 |
| α-helix | 555-569 | 15 | |
| α-helix | 653-666 | 14 | |
| β-strand | 670 | 1 | 22 |
| α-helix | 673-687 | 15 | |
| α-helix | 700-715 | 16 | |
| β-strand | 720 | 1 | 22 |
| α-helix | 723-737 | 15 | |
Chain 4: 23 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 189-199 | 11 | |
| α-helix | 204-210 | 7 | |
| α-helix | 225-236 | 12 | |
| β-strand | 241-244 | 4 | 23 |
| α-helix | 245-249 | 5 | |
| α-helix | 254-263 | 10 | |
| α-helix | 265-284 | 20 | |
| β-strand | 303-306 | 4 | 23 |
| β-strand | 311-312 | 2 | 24 |
| α-helix | 319-321 | 3 | |
| β-strand | 326-334 | 9 | 24 |
| β-strand | 339-349 | 11 | 24 |
| β-strand | 354-359 | 6 | 24 |
| β-strand | 361 | 1 | 25 |
| β-strand | 364 | 1 | 25 |
| β-strand | 382 | 1 | 24 |
| β-strand | 390-400 | 11 | 24 |
| α-helix | 403-405 | 3 | |
| β-strand | 414-420 | 7 | 24 |
| α-helix | 421-423 | 3 | |
| β-strand | 432-443 | 12 | 24 |
| β-strand | 453-454 | 2 | 26 |
| β-strand | 456 | 1 | 27 |
| β-strand | 457-468 | 12 | 24 |
| α-helix | 506-513 | 8 | |
| α-helix | 518-525 | 8 | |
| α-helix | 534-545 | 12 | |
| β-strand | 550 | 1 | 28 |
| β-strand | 558 | 1 | 28 |
| β-strand | 563-568 | 6 | 29 |
| α-helix | 574-584 | 11 | |
| β-strand | 590-592 | 3 | 29 |
| β-strand | 604-607 | 4 | 30 |
| β-strand | 614-617 | 4 | 30 |
| α-helix | 620-623 | 4 | |
| β-strand | 628-632 | 5 | 29 |
| α-helix | 640-649 | 10 | |
| β-strand | 653-658 | 6 | 31 |
| β-strand | 661-666 | 6 | 31 |
| β-strand | 670-675 | 6 | 29 |
| α-helix | 688-692 | 5 | |
| α-helix | 696-700 | 5 | |
| β-strand | 704-708 | 5 | 29 |
| α-helix | 714-728 | 15 | |
| α-helix | 746-757 | 12 | |
| β-strand | 761 | 1 | 32 |
| α-helix | 764-780 | 17 | |
| α-helix | 795-810 | 16 | |
| α-helix | 811-813 | 3 | |
| β-strand | 816 | 1 | 32 |
| α-helix | 819-834 | 16 | |
Chain 5: 29 helices, 26 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 21-37 | 17 | |
| β-strand | 40 | 1 | 33 |
| β-strand | 43 | 1 | 33 |
| α-helix | 45-55 | 11 | |
| β-strand | 60-64 | 5 | 34 |
| α-helix | 65-71 | 7 | |
| α-helix | 73-81 | 9 | |
| α-helix | 83-102 | 20 | |
| α-helix | 134-135 | 2 | |
| β-strand | 136-140 | 5 | 34 |
| α-helix | 143-145 | 3 | |
| α-helix | 148-150 | 3 | |
| α-helix | 153-155 | 3 | |
| β-strand | 159-169 | 11 | 35 |
| α-helix | 170-172 | 3 | |
| β-strand | 173-183 | 11 | 35 |
| β-strand | 189-193 | 5 | 35 |
| β-strand | 241-259 | 19 | 35 |
| α-helix | 262-264 | 3 | |
| β-strand | 273-279 | 7 | 35 |
| α-helix | 280-282 | 3 | |
| β-strand | 291-301 | 11 | 35 |
| β-strand | 325-335 | 11 | 35 |
| α-helix | 351-361 | 11 | |
| α-helix | 366-373 | 8 | |
| α-helix | 383-393 | 11 | |
| β-strand | 398-399 | 2 | 36 |
| β-strand | 405-406 | 2 | 36 |
| β-strand | 412-415 | 4 | 37 |
| α-helix | 422-432 | 11 | |
| β-strand | 436-440 | 5 | 37 |
| β-strand | 444 | 1 | 11 |
| β-strand | 451-455 | 5 | 38 |
| β-strand | 462-466 | 5 | 38 |
| α-helix | 470-472 | 3 | |
| β-strand | 475-480 | 6 | 37 |
| α-helix | 487-490 | 4 | |
| α-helix | 493-498 | 6 | |
| β-strand | 501-506 | 6 | 19 |
| β-strand | 509-514 | 6 | 19 |
| β-strand | 517-523 | 7 | 37 |
| α-helix | 544-549 | 6 | |
| β-strand | 552-554 | 3 | 37 |
| α-helix | 563-575 | 13 | |
| α-helix | 577-589 | 13 | |
| α-helix | 596-609 | 14 | |
| α-helix | 616-640 | 25 | |
| α-helix | 650-666 | 17 | |
| α-helix | 674-689 | 16 | |
| α-helix | 709-721 | 13 | |
| β-strand | 727-729 | 3 | 39 |
| α-helix | 730-739 | 10 | |
| α-helix | 743-757 | 15 | |
| β-strand | 762-764 | 3 | 39 |
| β-strand | 769-771 | 3 | 39 |
Chain 6: 27 helices, 22 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 100-104 | 5 | |
| α-helix | 106-120 | 15 | |
| α-helix | 135-146 | 12 | |
| β-strand | 150-154 | 5 | 40 |
| α-helix | 155-159 | 5 | |
| α-helix | 163-165 | 3 | |
| α-helix | 167-171 | 5 | |
| α-helix | 174-192 | 19 | |
| β-strand | 264-268 | 5 | 40 |
| β-strand | 274 | 1 | 6 |
| α-helix | 276-278 | 3 | |
| α-helix | 281-283 | 3 | |
| β-strand | 287-296 | 10 | 6 |
| β-strand | 301-311 | 11 | 6 |
| β-strand | 316-322 | 7 | 6 |
| β-strand | 344-346 | 3 | 6 |
| β-strand | 352-362 | 11 | 6 |
| α-helix | 372-375 | 4 | |
| β-strand | 376-382 | 7 | 6 |
| β-strand | 394-405 | 12 | 6 |
| β-strand | 450-461 | 12 | 6 |
| α-helix | 501-506 | 6 | |
| α-helix | 510-520 | 11 | |
| α-helix | 525-529 | 5 | |
| α-helix | 530-532 | 3 | |
| α-helix | 541-552 | 12 | |
| β-strand | 558 | 1 | 41 |
| β-strand | 564 | 1 | 41 |
| β-strand | 571-575 | 5 | 42 |
| α-helix | 582-591 | 10 | |
| β-strand | 596-599 | 4 | 42 |
| β-strand | 610-613 | 4 | 43 |
| β-strand | 622-625 | 4 | 43 |
| α-helix | 627-630 | 4 | |
| β-strand | 634-639 | 6 | 42 |
| α-helix | 641-643 | 3 | |
| α-helix | 646-658 | 13 | |
| β-strand | 660-665 | 6 | 9 |
| β-strand | 668-673 | 6 | 9 |
| β-strand | 676-682 | 7 | 42 |
| α-helix | 695-698 | 4 | |
| α-helix | 703-708 | 6 | |
| β-strand | 711-715 | 5 | 42 |
| α-helix | 721-732 | 12 | |
| α-helix | 733-737 | 5 | |
| α-helix | 748-758 | 11 | |
| α-helix | 767-785 | 19 | |
| α-helix | 797-813 | 17 | |
| α-helix | 821-839 | 19 | |
Chain 7: 31 helices, 22 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 7-8 | 2 | 15 |
| α-helix | 14-27 | 14 | |
| α-helix | 62-71 | 10 | |
| β-strand | 77-81 | 5 | 44 |
| α-helix | 82-92 | 11 | |
| α-helix | 103-108 | 6 | |
| α-helix | 111-123 | 13 | |
| α-helix | 126-128 | 3 | |
| α-helix | 138-153 | 16 | |
| α-helix | 194-197 | 4 | |
| β-strand | 201-205 | 5 | 44 |
| α-helix | 209-214 | 6 | |
| β-strand | 225 | 1 | 27 |
| α-helix | 232-234 | 3 | |
| β-strand | 238-248 | 11 | 27 |
| β-strand | 252-261 | 10 | 27 |
| α-helix | 268-269 | 2 | |
| β-strand | 270-272 | 3 | 27 |
| β-strand | 277-278 | 2 | 26 |
| α-helix | 287-290 | 4 | |
| β-strand | 299-316 | 18 | 27 |
| α-helix | 323 | 1 | |
| β-strand | 330-336 | 7 | 27 |
| β-strand | 348-358 | 11 | 27 |
| β-strand | 371 | 1 | 15 |
| β-strand | 373-382 | 10 | 27 |
| α-helix | 389-391 | 3 | |
| α-helix | 395-406 | 12 | |
| α-helix | 411-418 | 8 | |
| α-helix | 426-437 | 12 | |
| α-helix | 445-447 | 3 | |
| β-strand | 456-460 | 5 | 45 |
| α-helix | 466-476 | 11 | |
| β-strand | 480-483 | 4 | 45 |
| β-strand | 492 | 1 | 46 |
| β-strand | 513 | 1 | 46 |
| β-strand | 517-524 | 8 | 45 |
| α-helix | 531-539 | 9 | |
| α-helix | 541-543 | 3 | |
| α-helix | 544-545 | 2 | |
| β-strand | 546 | 1 | 47 |
| β-strand | 557 | 1 | 47 |
| β-strand | 560-567 | 8 | 45 |
| α-helix | 579-581 | 3 | |
| α-helix | 588-593 | 6 | |
| β-strand | 596-600 | 5 | 45 |
| α-helix | 606-622 | 17 | |
| α-helix | 635-648 | 14 | |
| α-helix | 654-656 | 3 | |
| α-helix | 657-671 | 15 | |
| α-helix | 674-676 | 3 | |
| α-helix | 686-702 | 17 | |
| α-helix | 710-725 | 16 | |
Chain C: 9 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-11 | 6 | |
| β-strand | 15-19 | 5 | 48 |
| β-strand | 24 | 1 | 49 |
| β-strand | 38 | 1 | 49 |
| β-strand | 43-47 | 5 | 48 |
| α-helix | 48-57 | 10 | |
| β-strand | 73-74 | 2 | 48 |
| α-helix | 75-77 | 3 | |
| α-helix | 82-90 | 9 | |
| α-helix | 97-100 | 4 | |
| α-helix | 104-114 | 11 | |
| α-helix | 118-140 | 23 | |
| α-helix | 160-166 | 7 | |
| α-helix | 173-191 | 19 | |
Chain D: 10 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 56-72 | 17 | |
| α-helix | 81-111 | 31 | |
| α-helix | 116-118 | 3 | |
| α-helix | 123-153 | 31 | |
| α-helix | 155-162 | 8 | |
| α-helix | 171-174 | 4 | |
| α-helix | 177-194 | 18 | |
| α-helix | 195-199 | 5 | |
| α-helix | 204-206 | 3 | |
| β-strand | 227-232 | 6 | 50 |
| β-strand | 247-249 | 3 | 51 |
| β-strand | 252-260 | 9 | 51 |
| β-strand | 265-270 | 6 | 51 |
| β-strand | 274-278 | 5 | 50 |
| α-helix | 283-287 | 5 | |
| β-strand | 291-293 | 3 | 50 |
5 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| DNA replication licensing factor MCM2 | 2 | protein | 868 | Saccharomyces cerevisiae | P29469 (AlphaFold model) |
| DNA replication licensing factor MCM3 | 3 | protein | 1006 | Saccharomyces cerevisiae | P24279 (AlphaFold model) |
| DNA replication licensing factor MCM4 | 4 | protein | 933 | Saccharomyces cerevisiae | P30665 (AlphaFold model) |
| Minichromosome maintenance protein 5 | 5 | protein | 775 | Saccharomyces cerevisiae | P29496 (AlphaFold model) |
| DNA replication licensing factor MCM6 | 6 | protein | 1017 | Saccharomyces cerevisiae | P53091 |
| DNA replication licensing factor MCM7 | 7 | protein | 845 | Saccharomyces cerevisiae | P38132 |
| DNA (9-mer) | A | DNA | 9 | synthetic construct | |
| DNA replication complex GINS protein PSF3 | C | protein | 229 | Saccharomyces cerevisiae | Q12146 |
| DNA replication complex GINS protein SLD5 | D | protein | 294 | Saccharomyces cerevisiae | Q03406 |
| Cell division control protein 45 | E | protein | 657 | Saccharomyces cerevisiae | Q08032 |
| DNA polymerase epsilon subunit B | F | protein | 689 | Saccharomyces cerevisiae | P24482 |
| DNA polymerase epsilon catalytic subunit A | G | protein | 2222 | Saccharomyces cerevisiae | P21951 |
2 more molecules are not listed.
Sequence of entity 1 (2), FASTA
>8P62_1 DNA replication licensing factor MCM2 (chains 2)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH
Sequence of entity 2 (3), FASTA
>8P62_2 DNA replication licensing factor MCM3 (chains 3)
MKRRWKKNFIAVSAANRFKKISSSGALENLYFQGEMEGSTGFDGDATTFFAPDAVFGDRV
RRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQDDADERDLLGDDDGDDLEKEKKAAS
STSLNILPHRIIISLDDLREFDRSFWSGILVEPAYFIPPAEKALTDLADSMDDVPHPNAS
AVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLVSVEGIVTKTSLVRPKLIRSVHYAAK
TGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNKLTTEYGYSTFIDHQRITVQEMPEMA
PAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKSLGAGGMNQSNSNTLIGFKTLILGNT
VYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIFDILSQSLAPSIYGHDHIKKAILLML
MGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLRFVLNTASLAIATTGRGSSGVGLTAA
VTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTDVDRVAIHEVMEQQTVTIAKAGIHTT
LNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLLSRFDLLFVVTDDINEIRDRSISEHV
LRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINPEEHSNSGAGVENEGEDDEDHVFEKF
NPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYVQYAKERVIPQLTQEAINVIVKNYTD
LRNDDNTKKSPITARTLETLIRLATAHAKVRLSKTVNKVDAKVAANLLRFALLGEDIGND
IDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSGSPIKSTPRRSTASSVNATPSSARRI
LRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQLGLRVSPRRREHLHAPEEGSSGPLTE
VGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTISTGRLSLISGIIARLMQTEIFEEESYP
VASLFERINEELPEEEKFSAQEYLAGLKIMSDRNNLMVADDKVWRV
Sequence of entity 3 (4), FASTA
>8P62_3 DNA replication licensing factor MCM4 (chains 4)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV
Sequence of entity 4 (5), FASTA
>8P62_4 Minichromosome maintenance protein 5 (chains 5)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV
Sequence of entity 5 (6), FASTA
>8P62_5 DNA replication licensing factor MCM6 (chains 6)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS
Sequence of entity 6 (7), FASTA
>8P62_6 DNA replication licensing factor MCM7 (chains 7)
MSAALPSIQLPVDYNNLFNEITDFLVTFKQDTLSSDATRNENEDENLDAENIEQHLLEKG
PKYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRA
IDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDA
LREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLIT
VRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFM
STRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPY
TGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAP
EIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRG
VYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEV
MEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLM
LDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYV
VQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLV
RVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLS
NCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANVSAQDSDI
DLQDA
Sequence of entity 7 (A), FASTA
>8P62_7 DNA (9-MER) (chains A)
AAAAAAAAA
Sequence of entity 8 (C), FASTA
>8P62_8 DNA replication complex GINS protein PSF3 (chains C)
WSHPQFEKGGGSGGGSGGGSWSHPQFEKENLYFQSMGYYDIDDVLADGTEFPCKFQYDIP
GLGYLENNPGRPITKNTKLSLPLWLARILAIVGGDEALVDEEPVPFVELLPPDMFSTKVM
NAIKTDPVALDLHSINSHFFSLAIKWIMLFSEKELANVVSELLLQRAQELNHHASSLSID
LNADSTGKNSANTNIATSTFLLKLEEMEKEIYKKSHESYKDTKRWMFKK
Sequence of entity 9 (D), FASTA
>8P62_9 DNA replication complex GINS protein SLD5 (chains D)
MDINIDDILAELDKETTAVDSTKITQGSSSTTHRDANTIVGSSLDLNDKTQIYVSPQQDF
SDLMKSWKNERCSPELLPYPHQLMKRLLNRISMQSQLIENISMGFLDMQNASNANPPMPN
ESKLPLLCMETELERLKFVIRSYIRCRLSKIDKFSLYLRQLNEDENSLISLTDLLSKDEI
KYHDTHSLIWLKLVNDSILKYMPEELQAINDTEGSVNMIDEPDWNKFVFIHVNGPPDGKW
NEDPLLQENEFGKPCYTVTIPDLKEEVELTIGSIYVMRYEVIRDLLRDDKVALI
Sequence of entity 10 (E), FASTA
>8P62_10 Cell division control protein 45 (chains E)
MYYGISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFKKQLVQSQIVP
IFGYSELRRHYSQLDDNINSLLLVGFGGVIDLEAFLEIDPQEYVIDTDEKSGEQSFRRDI
YVLDAHRPWNLDNIFGSQIIQCFDDGTVDDTLGEQKEAYYKLLELDEESGDDELSGDEND
NNGGDDEATDADEVTDEDYKDDDGDYKDDDETISNKRGNSSIGPNDLSKRKQRKKQIHEY
EGVLEEYYSQGTTVVNSISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYP
LLQDEVKRLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSLWNE
NGKKRLHKMFARMGIPLSTAQETWLYMDHSIKRELGIIFDKNLDRYGLQDIIRDGFVRTL
GYRGSISASEFVEALTALLEVGNSTDKDSVKINNDNNDDTDGEEEEDNSAQKLTNLRKRW
VSNFWLSWDALDDRKVELLNRGIQLAQDLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDG
PDLDLYRNPLTLLRLGNWLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGLD
TIHTKKPILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL
Sequence of entity 11 (F), FASTA
>8P62_11 DNA polymerase epsilon subunit B (chains F)
MFGSGNVLPVKIQPPLLRPLAYRVLSRKYGLSIKSDGLSALAEFVGTNIGANWRQGPATI
KFLEQFAAVWKQQERGLFIDQSGVKEVIQEMKEREKVEWSHEHPIQHEENILGRTDDDEN
NSDDEMPIAADSSLQNVSLSSPMRQPTERDEYKQPFKPESSKALDWRDYFKVINASQQQR
FSYNPHKMQFIFVPNKKQNGLGGIAGFLPDIEDKVQMFLTRYYLTNDRVMRNENFQNSDM
FNPLSSMVSLQNELSNTNRQQQSSSMSITPIKNLLGRDAQNFLLLGLLNKNFKGNWSLED
PSGSVEIDISQTIPTQGHYYVPGCMVLVEGIYYSVGNKFHVTSMTLPPGERREITLETIG
NLDLLGIHGISNNNFIARLDKDLKIRLHLLEKELTDHKFVILGANLFLDDLKIMTALSKI
LQKLNDDPPTLLIWQGSFTSVPVFASMSSRNISSSTQFKNNFDALATLLSRFDNLTENTT
MIFIPGPNDLWGSMVSLGASGTLPQDPIPSAFTKKINKVCKNVVWSSNPTRIAYLSQEIV
IFRDDLSGRFKRHRLEFPFNESEDVYTENDNMMSKDTDIVPIDELVKEPDQLPQKVQETR
KLVKTILDQGHLSPFLDSLRPISWDLDHTLTLCPIPSTMVLCDTTSAQFDLTYNGCKVIN
PGSFIHNRRARYMEYVPSSKKTIQEEIYI
Sequence of entity 12 (G), FASTA
>8P62_12 DNA polymerase epsilon catalytic subunit A (chains G)
MMFGKKKNNGGSSTARYSAGNKYNTLSNNYALSAQQLLNASKIDDIDSMMGFERYVPPQY
NGRFDAKDIDQIPGRVGWLTNMHATLVSQETLSSGSNGGGNSNDGERVTTNQGISGVDFY
FLDEEGGSFKSTVVYDPYFFIACNDESRVNDVEELVKKYLESCLKSLQIIRKEDLTMDNH
LLGLQKTLIKLSFVNSNQLFEARKLLRPILQDNANNNVQRNIYNVAANGSEKVDAKHLIE
DIREYDVPYHVRVSIDKDIRVGKWYKVTQQGFIEDTRKIAFADPVVMAFDIETTKPPLKF
PDSAVDQIMMISYMIDGEGFLITNREIISEDIEDFEYTPKPEYPGFFTIFNENDEVALLQ
RFFEHIRDVRPTVISTFNGDFFDWPFIHNRSKIHGLDMFDEIGFAPDAEGEYKSSYCSHM
DCFRWVKRDSYLPQGSQGLKAVTQSKLGYNPIELDPELMTPYAFEKPQHLSEYSVSDAVA
TYYLYMKYVHPFIFSLCTIIPLNPDETLRKGTGTLCEMLLMVQAYQHNILLPNKHTDPIE
RFYDGHLLESETYVGGHVESLEAGVFRSDLKNEFKIDPSAIDELLQELPEALKFSVEVEN
KSSVDKVTNFEEIKNQITQKLLELKENNIRNELPLIYHVDVASMYPNIMTTNRLQPDSIK
AERDCASCDFNRPGKTCARKLKWAWRGEFFPSKMDEYNMIKRALQNETFPNKNKFSKKKV
LTFDELSYADQVIHIKKRLTEYSRKVYHRVKVSEIVEREAIVCQRENPFYVDTVKSFRDR
RYEFKGLAKTWKGNLSKIDPSDKHARDEAKKMIVLYDSLQLAHKVILNSFYGYVMRKGSR
WYSMEMAGITCLTGATIIQMARALVERVGRPLELDTDGIWCILPKSFPETYFFTLENGKK
LYLSYPCSMLNYRVHQKFTNHQYQELKDPLNYIYETHSENTIFFEVDGPYKAMILPSSKE
EGKGIKKRYAVFNEDGSLAELKGFELKRRGELQLIKNFQSDIFKVFLEGDTLEGCYSAVA
SVCNRWLDVLDSHGLMLEDEDLVSLICENRSMSKTLKEYEGQKSTSITTARRLGDFLGED
MVKDKGLQCKYIISSKPFNAPVTERAIPVAIFSADIPIKRSFLRRWTLDPSLEDLDIRTI
IDWGYYRERLGSAIQKIITIPAALQGVSNPVPRVEHPDWLKRKIATKEDKFKQTSLTKFF
SKTKNVPTMGKIKDIEDLFEPTVEEDNAKIKIARTTKKKAVSKRKRNQLTNEEDPLVLPS
EIPSMDEDYVGWLNYQKIKWKIQARDRKRRDQLFGNTNSSRERSALGSMIRKQAESYANS
TWEVLQYKDSGEPGVLEVFVTINGKVQNITFHIPKTIYMKFKSQTMPLQKIKNCLIEKSS
ASLPNNPKTSNPAGGQLFKITLPESVFLEEKENCTSIFNDENVLGVFEGTITPHQRAIMD
LGASVTFRSKAMGALGKGIQQGFEMKDLSMAENERYLSGFSMDIGYLLHFPTSIGYEFFS
LFKSWGDTITILVLKPSNQAQEINASSLGQIYKQMFEKKKGKIETYSYLVDIKEDINFEF
VYFTDISKLYRRLSQETTKLKEERGLQFLLLLQSPFITKLLGTIRLLNQMPIVKLSLNEV
LLPQLNWQPTLLKKLVNHVLSSGSWISHLIKLSQYSNIPICNLRLDSMDYIIDVLYARKL
KKENIVLWWNEKAPLPDHGGIQNDFDLNTSWIMNDSEFPKINNSGVYDNVVLDVGVDNLT
VNTILTSALINDAEGSDLVNNNMGIDDKDAVINSPSEFVHDAFSNDALNVLRGMLKEWWD
EALKENSTADLLVNSLASWVQNPNAKLFDGLLRYHVHNLTKKALLQLVNEFSALGSTIVY
ADRNQILIKTNKYSPENCYAYSQYMMKAVRTNPMFSYLDLNIKRYWDLLIWMDKFNFSGL
ACIEIEEKENQDYTAVSQWQLKKFLSPIYQPEFEDWMMIILDSMLKTKQSYLKLNSGTQR
PTQIVNVKKQDKEDSVENSLNGFSHLFSKPLMKRVKKLFKNQQEFILDPQYEADYVIPVL
PGSHLNVKNPLLELVKSLCHVMLLSKSTILEIRTLRKELLKIFELREFAKVAEFKDPSLS
LVVPDFLCEYCFFISDIDFCKAAPESIFSCVRCHKAFNQVLLQEHLIQKLRSDIESYLIQ
DLRCSRCHKVKRDYMSAHCPCAGAWEGTLPRESIVQKLNVFKQVAKYYGFDILLSCIADL
TI
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ATP | Adenosine-5'-triphosphate | C10 H16 N5 O13 P3 | 4 |
| MG | Magnesium ion | Mg | 3 |
| ADP | Adenosine-5'-diphosphate | C10 H15 N5 O10 P2 | 2 |
| ZN | Zinc ion | Zn | 7 |
Primary citation
Unwinding of a eukaryotic origin of replication visualized by cryo-EM. Henrikus, S.S., Gross, M.H., Willhoft, O. et al. Nat Struct Mol Biol (2024) 31:1265-1276. DOI 10.1038/s41594-024-01280-z · PubMed
Other PDB entries of the same protein (UniProt P29469 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7W8G 2.52 Å, Cryo-EM structure of MCM double hexamer
- 28VY 2.7 Å, sCMGE assembled on ARS1 DNA with Sld2 and RPA
- 8RIF 2.79 Å, Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
- 7V3V 2.9 Å, Cryo-EM structure of MCM double hexamer bound with DDK in State I
- 9TH8 2.9 Å, Cryo-EM structure of MCM2-7 DH bound to Sld3-Sld7, Cdc45 and DNA
- 7P30 3.0 Å, 3.0 A resolution structure of a DNA-loaded MCM double hexamer
- 8KG6 3.07 Å, Yeast replisome in state I
- 9RHL 3.1 Å, Phospho-DH bound by Sld3-MBD on ARS1 DNA
- 7PMK 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
- 7PMN 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
- 7PT6 3.2 Å, Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III
- 7V3U 3.2 Å, Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD
Browse structure collections
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