8RIG: MCM helicase single hexamer loaded onto dsDNA

Name: PDB 8RIG: Cryo-EM structure of an MCM helicase single hexamer loaded onto dsDNA.
Published: 2025-01-01
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: replication

Cryo-EM structure of an MCM helicase single hexamer loaded onto dsDNA. Determined by electron microscopy at 3.41 Å resolution. Released 1 Jan 2025.

Method: Electron microscopy
Resolution: 3.41 Å
Organisms: Saccharomyces cerevisiae S288C, Saccharomyces cerevisiae
Chains: 8
Atoms: 30,208
Mol. weight: 624.65 kDa
Ligands: ATP, MG, ZN, ADP
Released: 1 Jan 2025

Explore 8RIG in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8RIG contains 176 α-helices and 176 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 2: 30 helices, 27 β-strands

Element	Residues	Length	Sheet
α-helix	183-186	4
α-helix	194-199	6
α-helix	201-217	17
α-helix	226-237	12
β-strand	241-245	5	1
α-helix	246-251	6
α-helix	254-262	9
α-helix	264-282	19
α-helix	286-288	3
β-strand	294-298	5	1
β-strand	304	1	2
α-helix	311-313	3
β-strand	317-327	11	2
β-strand	331-336	6	2
β-strand	339-341	3	3
β-strand	347-348	2	3
α-helix	349-350	2
β-strand	352	1	2
β-strand	373-375	3	3
β-strand	381-391	11	2
α-helix	398	1
α-helix	401-404	4
β-strand	405-411	7	2
β-strand	423-432	10	2
β-strand	446	1	4
β-strand	449-458	10	2
α-helix	478-488	11
α-helix	493-499	7
α-helix	509-520	12
β-strand	525-526	2	5
β-strand	532-533	2	5
β-strand	539-543	5	6
α-helix	550-559	10
β-strand	563-567	5	6
β-strand	579-582	4	7
β-strand	589-592	4	7
α-helix	595-598	4
β-strand	603-607	5	6
α-helix	614-626	13
β-strand	628-633	6	8
β-strand	636-641	6	8
β-strand	645-650	6	6
α-helix	653-655	3
α-helix	663-666	4
α-helix	671-674	4
β-strand	679-683	5	6
α-helix	691-704	14
α-helix	757-759	3
α-helix	760-773	14
β-strand	777	1	9
α-helix	780-782	3
α-helix	783-798	16
α-helix	804-805	2
α-helix	807-823	17
β-strand	828	1	9
α-helix	831-846	16
α-helix	851-861	11

Chain 3: 32 helices, 26 β-strands

Element	Residues	Length	Sheet
α-helix	19-34	16
α-helix	37-47	11
α-helix	91-93	3
β-strand	95-99	5	10
α-helix	100-106	7
α-helix	108-116	9
α-helix	118-120	3
α-helix	122-135	14
β-strand	154-158	5	10
α-helix	162-164	3
β-strand	165-166	2	11
α-helix	173-175	3
β-strand	179-188	10	11
β-strand	193-203	11	11
β-strand	208-213	6	11
α-helix	217-219	3
β-strand	234	1	12
β-strand	240	1	12
β-strand	242-243	2	11
α-helix	245-247	3
β-strand	249-259	11	11
α-helix	266	1
β-strand	273-279	7	11
α-helix	280-282	3
β-strand	291-301	11	11
β-strand	317	1	13
β-strand	318-330	13	11
α-helix	337-338	2
α-helix	344-353	10
α-helix	359-365	7
α-helix	375-386	12
β-strand	391-392	2	14
β-strand	398-399	2	14
β-strand	405-409	5	15
α-helix	416-425	10
β-strand	429-432	4	15
α-helix	439-442	4
β-strand	447-449	3	16
β-strand	455-457	3	16
α-helix	458-459	2
α-helix	461-464	4
β-strand	469-472	4	15
α-helix	475-477	3
α-helix	480-483	4
α-helix	487-492	6
β-strand	494-499	6	17
β-strand	502-507	6	17
β-strand	511-516	6	15
α-helix	529-533	5
α-helix	537-542	6
β-strand	545-549	5	15
α-helix	556-568	13
α-helix	580-582	3
β-strand	589-590	2	11
α-helix	653-666	14
α-helix	673-687	15
α-helix	699-715	17
α-helix	723-737	15

Chain 4: 30 helices, 32 β-strands

Element	Residues	Length	Sheet
α-helix	188-197	10
α-helix	225-236	12
β-strand	240-244	5	18
α-helix	245-250	6
α-helix	253-255	3
α-helix	256-263	8
α-helix	265-286	22
α-helix	296-298	3
β-strand	302-306	5	18
α-helix	319-321	3
β-strand	325-335	11	19
β-strand	341-349	9	20
β-strand	355-359	5	20
β-strand	361	1	21
β-strand	364-365	2	21
α-helix	366-368	3
β-strand	370	1	22
β-strand	379	1	22
β-strand	382-384	3	20
α-helix	386-388	3
β-strand	390-392	3	20
β-strand	394-401	8	19
α-helix	402	1
β-strand	414-420	7	19
β-strand	432-442	11	19
β-strand	445	1	23
β-strand	453-454	2	23
β-strand	456	1	24
α-helix	457	1
β-strand	458-467	10	19
α-helix	474-477	4
α-helix	503-512	10
α-helix	518-525	8
α-helix	534-545	12
α-helix	547-549	3
β-strand	564-568	5	25
α-helix	574-583	10
β-strand	590-591	2	25
β-strand	596	1	26
α-helix	598-602	5
β-strand	603-607	5	27
β-strand	614-618	5	27
β-strand	628-631	4	25
α-helix	645-648	4
β-strand	653-658	6	28
β-strand	661-666	6	28
β-strand	670-675	6	25
α-helix	676-677	2
α-helix	688-691	4
α-helix	696-699	4
β-strand	704-708	5	25
α-helix	714-725	12
α-helix	726-728	3
α-helix	744-757	14
β-strand	761-762	2	29
α-helix	764-779	16
α-helix	795-811	17
β-strand	816-817	2	29
α-helix	819-832	14
β-strand	838	1	30
β-strand	845	1	30
α-helix	847-849	3

Chain 5: 28 helices, 32 β-strands

Element	Residues	Length	Sheet
α-helix	26-36	11
β-strand	40	1	31
β-strand	43	1	31
α-helix	45-53	9
β-strand	60-64	5	32
α-helix	68-71	4
α-helix	73-80	8
β-strand	81	1	33
α-helix	83-104	22
α-helix	134-135	2
β-strand	136-140	5	32
α-helix	153-155	3
β-strand	158	1	33
β-strand	159-169	11	13
β-strand	173-183	11	13
β-strand	189-193	5	13
β-strand	241-243	3	13
α-helix	245-247	3
β-strand	249-259	11	13
α-helix	270-272	3
β-strand	273-279	7	13
α-helix	280-282	3
β-strand	291-301	11	13
β-strand	323	1	2
β-strand	325-337	13	13
α-helix	351-361	11
α-helix	366-372	7
α-helix	382-393	12
β-strand	398-399	2	34
β-strand	405-406	2	34
β-strand	412-416	5	35
α-helix	424-431	8
β-strand	436-440	5	35
β-strand	444	1	36
β-strand	447	1	36
β-strand	448	1	37
β-strand	453-455	3	38
β-strand	462-464	3	38
β-strand	467	1	37
α-helix	468-471	4
β-strand	476-480	5	35
α-helix	487-496	10
β-strand	503-505	3	39
β-strand	510-512	3	39
β-strand	518-523	6	35
α-helix	536-539	4
α-helix	544-546	3
β-strand	552-556	5	35
α-helix	564-577	14
α-helix	587-589	3
α-helix	596-609	14
β-strand	613-614	2	40
α-helix	616-629	14
α-helix	631-639	9
α-helix	643-644	2
α-helix	650-664	15
α-helix	665-667	3
β-strand	671-672	2	40
α-helix	674-687	14
α-helix	689-692	4

Chain 6: 27 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	101-103	3
α-helix	106-120	15
β-strand	123-124	2	41
β-strand	133-134	2	41
α-helix	135-146	12
β-strand	150-154	5	42
α-helix	155-158	4
α-helix	162-164	3
α-helix	165-172	8
α-helix	178-192	15
α-helix	194-196	3
β-strand	264-268	5	42
β-strand	274	1	4
α-helix	276-278	3
α-helix	281-283	3
β-strand	287-297	11	4
β-strand	301-311	11	4
β-strand	317-322	6	4
β-strand	344-346	3	4
β-strand	352-362	11	4
β-strand	376-382	7	4
α-helix	383-385	3
β-strand	394-405	12	4
β-strand	418-419	2	21
β-strand	446-447	2	21
β-strand	450-461	12	4
α-helix	511-520	10
α-helix	525-532	8
α-helix	541-551	11
β-strand	557-558	2	43
β-strand	564-565	2	43
β-strand	571-575	5	44
α-helix	582-589	8
β-strand	595-599	5	44
β-strand	611-614	4	45
β-strand	621-624	4	45
α-helix	627-630	4
β-strand	635-639	5	44
α-helix	641-643	3
α-helix	646-658	13
β-strand	660-664	5	46
β-strand	669-673	5	46
β-strand	677-682	6	44
α-helix	695-698	4
α-helix	703-706	4
β-strand	711-715	5	44
α-helix	723-737	15
α-helix	738-740	3
α-helix	748-759	12
β-strand	764	1	47
α-helix	769-782	14
α-helix	798-814	17
β-strand	818	1	47
α-helix	821-833	13
α-helix	836-838	3

Chain 7: 29 helices, 31 β-strands

Element	Residues	Length	Sheet
β-strand	7-8	2	11
α-helix	16-27	12
α-helix	62-71	10
β-strand	77-81	5	48
α-helix	82-95	14
α-helix	102-108	7
α-helix	113-121	9
α-helix	194-196	3
β-strand	201-205	5	48
α-helix	206-208	3
α-helix	209-215	7
β-strand	224-225	2	24
α-helix	232-234	3
β-strand	238-248	11	24
β-strand	252-255	4	24
β-strand	257-262	6	49
β-strand	268-272	5	49
β-strand	277-278	2	23
β-strand	283	1	50
α-helix	287-291	5
α-helix	295-296	2
β-strand	297	1	50
β-strand	298-300	3	49
β-strand	307-316	10	24
β-strand	330-336	7	24
β-strand	348-357	10	24
β-strand	371	1	11
β-strand	374-383	10	24
α-helix	388-390	3
α-helix	395-405	11
α-helix	411-416	6
α-helix	426-437	12
β-strand	442-443	2	51
β-strand	449-450	2	51
β-strand	456-460	5	52
α-helix	467-476	10
β-strand	481-484	4	52
α-helix	491-494	4
β-strand	495-499	5	53
β-strand	506-510	5	53
α-helix	512-515	4
β-strand	519-524	6	52
α-helix	526-528	3
α-helix	531-534	4
α-helix	537-543	7
β-strand	545-548	4	54
β-strand	549	1	26
β-strand	555-558	4	54
β-strand	561-567	7	52
α-helix	580-583	4
α-helix	588-592	5
β-strand	596-600	5	52
α-helix	608-621	14
α-helix	632-634	3
α-helix	635-645	11
β-strand	651	1	55
α-helix	654-677	24
α-helix	686-702	17
β-strand	707	1	55
α-helix	710-722	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA replication licensing factor MCM2	2	protein	868	Saccharomyces cerevisiae S288C	P29469 (AlphaFold model)
DNA replication licensing factor MCM3	3	protein	1006	Saccharomyces cerevisiae S288C	P24279 (AlphaFold model)
DNA replication licensing factor MCM4	4	protein	933	Saccharomyces cerevisiae S288C	P30665 (AlphaFold model)
Minichromosome maintenance protein 5	5	protein	775	Saccharomyces cerevisiae S288C	P29496 (AlphaFold model)
DNA replication licensing factor MCM6	6	protein	1017	Saccharomyces cerevisiae S288C	P53091
DNA replication licensing factor MCM7	7	protein	845	Saccharomyces cerevisiae S288C	P38132
DNA (5'-d(pgpcpaptpgpcpaptpgpcpaptpgpcpaptpgpcpapt)-3')	X	DNA	19	Saccharomyces cerevisiae
DNA (5'-d(paptpgpcpaptpgpcpaptpgpcpaptpgpcpapt)-3')	Y	DNA	17	Saccharomyces cerevisiae

Sequence of entity 1 (2), FASTA

>8RIG_1 DNA replication licensing factor MCM2 (chains 2)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH

Sequence of entity 2 (3), FASTA

>8RIG_2 DNA replication licensing factor MCM3 (chains 3)
MKRRWKKNFIAVSAANRFKKISSSGALENLYFQGEMEGSTGFDGDATTFFAPDAVFGDRV
RRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQDDADERDLLGDDDGDDLEKEKKAAS
STSLNILPHRIIISLDDLREFDRSFWSGILVEPAYFIPPAEKALTDLADSMDDVPHPNAS
AVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLVSVEGIVTKTSLVRPKLIRSVHYAAK
TGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNKLTTEYGYSTFIDHQRITVQEMPEMA
PAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKSLGAGGMNQSNSNTLIGFKTLILGNT
VYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIFDILSQSLAPSIYGHDHIKKAILLML
MGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLRFVLNTASLAIATTGRGSSGVGLTAA
VTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTDVDRVAIHEVMEQQTVTIAKAGIHTT
LNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLLSRFDLLFVVTDDINEIRDRSISEHV
LRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINPEEHSNSGAGVENEGEDDEDHVFEKF
NPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYVQYAKERVIPQLTQEAINVIVKNYTD
LRNDDNTKKSPITARTLETLIRLATAHAKVRLSKTVNKVDAKVAANLLRFALLGEDIGND
IDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSGSPIKSTPRRSTASSVNATPSSARRI
LRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQLGLRVSPRRREHLHAPEEGSSGPLTE
VGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTISTGRLSLISGIIARLMQTEIFEEESYP
VASLFERINEELPEEEKFSAQEYLAGLKIMSDRNNLMVADDKVWRV

Sequence of entity 3 (4), FASTA

>8RIG_3 DNA replication licensing factor MCM4 (chains 4)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV

Sequence of entity 4 (5), FASTA

>8RIG_4 Minichromosome maintenance protein 5 (chains 5)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV

Sequence of entity 5 (6), FASTA

>8RIG_5 DNA replication licensing factor MCM6 (chains 6)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS

Sequence of entity 6 (7), FASTA

>8RIG_6 DNA replication licensing factor MCM7 (chains 7)
MSAALPSIQLPVDYNNLFNEITDFLVTFKQDTLSSDATRNENEDENLDAENIEQHLLEKG
PKYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRA
IDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDA
LREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLIT
VRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFM
STRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPY
TGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAP
EIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRG
VYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEV
MEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLM
LDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYV
VQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLV
RVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLS
NCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANVSAQDSDI
DLQDA

Sequence of entity 7 (X), FASTA

>8RIG_7 DNA (5'-D(P*GP*CP*AP*TP*GP*CP*AP*TP*GP*CP*AP*TP*GP*CP*AP*TP*GP*CP*AP*T)-3') (chains X)
CATGCATGCATGCATGCAT

Sequence of entity 8 (Y), FASTA

>8RIG_8 DNA (5'-D(P*AP*TP*GP*CP*AP*TP*GP*CP*AP*TP*GP*CP*AP*TP*GP*CP*AP*T)-3') (chains Y)
ATGCATGCATGCATGCA

Ligands and cofactors

ID	Name	Formula	Copies
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	2
MG	Magnesium ion	Mg	6
ZN	Zinc ion	Zn	5
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	4

Primary citation

Cell cycle regulation has shaped replication origins in budding yeast. Lim, C.T., Miller, T.C.R., Tan, K.W. et al. Nat Struct Mol Biol (2025) 32:1697-1707. DOI 10.1038/s41594-025-01591-9 · PubMed

Other PDB entries of the same protein (UniProt P29469 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7W8G 2.52 Å, Cryo-EM structure of MCM double hexamer
28VY 2.7 Å, sCMGE assembled on ARS1 DNA with Sld2 and RPA
8RIF 2.79 Å, Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
7V3V 2.9 Å, Cryo-EM structure of MCM double hexamer bound with DDK in State I
9TH8 2.9 Å, Cryo-EM structure of MCM2-7 DH bound to Sld3-Sld7, Cdc45 and DNA
7P30 3.0 Å, 3.0 A resolution structure of a DNA-loaded MCM double hexamer
8KG6 3.07 Å, Yeast replisome in state I
9RHL 3.1 Å, Phospho-DH bound by Sld3-MBD on ARS1 DNA
7PMK 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
7PMN 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
7PT6 3.2 Å, Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III
7V3U 3.2 Å, Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD

Browse structure collections

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