8S0C: H. sapiens ORC1-5
H. sapiens ORC1-5 bound to double stranded DNA as part of the MCM-ORC complex. Determined by electron microscopy at 4.0 Å resolution. Released 2 Oct 2024.
- Method
- Electron microscopy
- Resolution
- 4.0 Å
- Organisms
- Homo sapiens, synthetic construct
- Chains
- 7
- Atoms
- 14,133
- Mol. weight
- 363.86 kDa
- Ligands
- AGS, MG
- Released
- 2 Oct 2024
Explore 8S0C in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8S0C contains 86 α-helices and 29 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 7 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 749-758 | 10 | |
| α-helix | 763-768 | 6 | |
| α-helix | 772-788 | 17 | |
| β-strand | 793 | 1 | 1 |
| α-helix | 795-809 | 15 | |
| α-helix | 811-815 | 5 | |
| α-helix | 816-828 | 13 | |
| β-strand | 832-833 | 2 | 2 |
| β-strand | 845 | 1 | 1 |
| β-strand | 846-847 | 2 | 2 |
| α-helix | 851-859 | 9 | |
Chain B: 11 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 271-275 | 5 | |
| α-helix | 284-295 | 12 | |
| α-helix | 298-306 | 9 | |
| β-strand | 310-314 | 5 | 3 |
| α-helix | 320-329 | 10 | |
| β-strand | 336-339 | 4 | 3 |
| α-helix | 348-353 | 6 | |
| α-helix | 354-359 | 6 | |
| α-helix | 370-382 | 13 | |
| β-strand | 388-392 | 5 | 3 |
| α-helix | 398-400 | 3 | |
| α-helix | 403-413 | 11 | |
| β-strand | 418-424 | 7 | 3 |
| α-helix | 429-432 | 4 | |
| α-helix | 435-440 | 6 | |
| β-strand | 443-447 | 5 | 3 |
Chain C: 30 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 26-30 | 5 | |
| α-helix | 37-84 | 48 | |
| β-strand | 100-104 | 5 | 4 |
| α-helix | 114-126 | 13 | |
| β-strand | 133-136 | 4 | 4 |
| α-helix | 138-140 | 3 | |
| α-helix | 144-158 | 15 | |
| α-helix | 181-192 | 12 | |
| β-strand | 216-219 | 4 | 4 |
| α-helix | 222-224 | 3 | |
| α-helix | 227-238 | 12 | |
| β-strand | 247-250 | 4 | 4 |
| α-helix | 258-262 | 5 | |
| α-helix | 265-268 | 4 | |
| β-strand | 271-275 | 5 | 4 |
| α-helix | 282-290 | 9 | |
| α-helix | 304-317 | 14 | |
| α-helix | 322-338 | 17 | |
| α-helix | 340-343 | 4 | |
| α-helix | 348-356 | 9 | |
| α-helix | 360-367 | 8 | |
| α-helix | 370-374 | 5 | |
| α-helix | 403-425 | 23 | |
| α-helix | 435-444 | 10 | |
| α-helix | 453-460 | 8 | |
| α-helix | 465-481 | 17 | |
| α-helix | 488-499 | 12 | |
| α-helix | 550-566 | 17 | |
| α-helix | 571-573 | 3 | |
| α-helix | 585-592 | 8 | |
| α-helix | 596-605 | 10 | |
| α-helix | 607-609 | 3 | |
| α-helix | 629-637 | 9 | |
| α-helix | 646-659 | 14 | |
| α-helix | 676-690 | 15 | |
Chain D: 18 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 18-28 | 11 | |
| α-helix | 41-56 | 16 | |
| β-strand | 61-66 | 6 | 5 |
| α-helix | 73-83 | 11 | |
| β-strand | 95-100 | 6 | 5 |
| α-helix | 107-117 | 11 | |
| β-strand | 154-159 | 6 | 5 |
| α-helix | 162-166 | 5 | |
| α-helix | 171-181 | 11 | |
| β-strand | 187-193 | 7 | 5 |
| α-helix | 204-209 | 6 | |
| β-strand | 214-216 | 3 | 5 |
| α-helix | 223-233 | 11 | |
| α-helix | 243-257 | 15 | |
| α-helix | 260-272 | 13 | |
| α-helix | 276-287 | 12 | |
| α-helix | 299-309 | 11 | |
| α-helix | 313-319 | 7 | |
| α-helix | 323-338 | 16 | |
| β-strand | 344 | 1 | 6 |
| α-helix | 346-359 | 14 | |
| α-helix | 369-381 | 13 | |
| β-strand | 402 | 1 | 6 |
| α-helix | 408-416 | 9 | |
| α-helix | 423-430 | 8 | |
Chain E: 20 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 13-22 | 10 | |
| β-strand | 32-36 | 5 | 7 |
| α-helix | 45-54 | 10 | |
| β-strand | 58-62 | 5 | 7 |
| α-helix | 69-84 | 16 | |
| α-helix | 99-109 | 11 | |
| β-strand | 120-125 | 6 | 7 |
| α-helix | 127-132 | 6 | |
| α-helix | 137-142 | 6 | |
| α-helix | 144-148 | 5 | |
| β-strand | 152-156 | 5 | 7 |
| α-helix | 162-164 | 3 | |
| α-helix | 173-174 | 2 | |
| β-strand | 175-178 | 4 | 7 |
| α-helix | 179-182 | 4 | |
| α-helix | 184-191 | 8 | |
| α-helix | 201-215 | 15 | |
| α-helix | 222-242 | 21 | |
| α-helix | 250-267 | 18 | |
| α-helix | 275-283 | 9 | |
| α-helix | 305-317 | 13 | |
| β-strand | 357 | 1 | 8 |
| α-helix | 359-369 | 11 | |
| α-helix | 378-388 | 11 | |
| β-strand | 393-395 | 3 | 8 |
| β-strand | 407-409 | 3 | 8 |
| α-helix | 413-422 | 10 | |
| α-helix | 428-430 | 3 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Origin recognition complex subunit 1 | A | protein | 861 | Homo sapiens | Q13415 (AlphaFold model) |
| DNA (26-mer) | X | DNA | 26 | synthetic construct | |
| DNA (26-mer) | Y | DNA | 26 | synthetic construct | |
| Origin recognition complex subunit 2 | B | protein | 577 | Homo sapiens | Q13416 (AlphaFold model) |
| Isoform 2 of Origin recognition complex subunit 3 | C | protein | 712 | Homo sapiens | Q9UBD5 (AlphaFold model) |
| Origin recognition complex subunit 4 | D | protein | 436 | Homo sapiens | O43929 (AlphaFold model) |
| Origin recognition complex subunit 5 | E | protein | 435 | Homo sapiens | O43913 |
Sequence of entity 1 (A), FASTA
>8S0C_1 Origin recognition complex subunit 1 (chains A)
MAHYPTRLKTRKTYSWVGRPLLDRKLHYQTYREMCVKTEGCSTEIHIQIGQFVLIEGDDD
ENPYVAKLLELFEDDSDPPPKKRARVQWFVRFCEVPACKRHLLGRKPGAQEIFWYDYPAC
DSNINAETIIGLVRVIPLAPKDVVPTNLKNEKTLFVKLSWNEKKFRPLSSELFAELNKPQ
ESAAKCQKPVRAKSKSAESPSWTPAEHVAKRIESRHSASKSRQTPTHPLTPRARKRLELG
NLGNPQMSQQTSCASLDSPGRIKRKVAFSEITSPSKRSQPDKLQTLSPALKAPEKTRETG
LSYTEDDKKASPEHRIILRTRIAASKTIDIREERTLTPISGGQRSSVVPSVILKPENIKK
RDAKEAKAQNEATSTPHRIRRKSSVLTMNRIRQQLRFLGNSKSDQEEKEILPAAEISDSS
SDEEEASTPPLPRRAPRTVSRNLRSSLKSSLHTLTKVPKKSLKPRTPRCAAPQIRSRSLA
AQEPASVLEEARLRLHVSAVPESLPCREQEFQDIYNFVESKLLDHTGGCMYISGVPGTGK
TATVHEVIRCLQQAAQANDVPPFQYIEVNGMKLTEPHQVYVQILQKLTGQKATANHAAEL
LAKQFCTRGSPQETTVLLVDELDLLWTHKQDIMYNLFDWPTHKEARLVVLAIANTMDLPE
RIMMNRVSSRLGLTRMCFQPYTYSQLQQILRSRLKHLKAFEDDAIQLVARKVAALSGDAR
RCLDICRRATEICEFSQQKPDSPGLVTIAHSMEAVDEMFSSSYITAIKNSSVLEQSFLRA
ILAEFRRSGLEEATFQQIYSQHVALCRMEGLPYPTMSETMAVCSHLGSCRLLLVEPSRND
LLLRVRLNVSQDDVLYALKDE
Sequence of entity 2 (X), FASTA
>8S0C_2 DNA (26-mer) (chains X)
TATTTTCCCTTGACTGACTGACTGAA
Sequence of entity 3 (Y), FASTA
>8S0C_3 DNA (26-mer) (chains Y)
TTCAGTCAGTCAGTCAAGGGAAAATA
Sequence of entity 4 (B), FASTA
>8S0C_4 Origin recognition complex subunit 2 (chains B)
MSKPELKEDKMLEVHFVGDDDVLNHILDREGGAKLKKERAQLLVNPKKIIKKPEYDLEED
DQEVLKDQNYVEIMGRDVQESLKNGSATGGGNKVYSFQNRKHSEKMAKLASELAKTPQKS
VSFSLKNDPEITINVPQSSKGHSASDKVQPKNNDKSEFLSTAPRSLRKRLIVPRSHSDSE
SEYSASNSEDDEGVAQEHEEDTNAVIFSQKIQAQNRVVSAPVGKETPSKRMKRDKTSDLV
EEYFEAHSSSKVLTSDRTLQKLKRAKLDQQTLRNLLSKVSPSFSAELKQLNQQYEKLFHK
WMLQLHLGFNIVLYGLGSKRDLLERFRTTMLQDSIHVVINGFFPGISVKSVLNSITEEVL
DHMGTFRSILDQLDWIVNKFKEDSSLELFLLIHNLDSQMLRGEKSQQIIGQLSSLHNIYL
IASIDHLNAPLMWDHAKQSLFNWLWYETTTYSPYTEETSYENSLLVKQSGSLPLSSLTHV
LRSLTPNARGIFRLLIKYQLDNQDNPSYIGLSFQDFYQQCREAFLVNSDLTLRAQLTEFR
DHKLIRTKKGTDGVEYLLIPVDNGTLTDFLEKEEEEA
Sequence of entity 5 (C), FASTA
>8S0C_5 Isoform 2 of Origin recognition complex subunit 3 (chains C)
MATSSMSKGCFVFKPNSKKRKISLPIEDYFNKGKNEPEDSKLRFETYQLIWQQMKSENER
LQEELNKNLFDNLIEFLQKSHSGFQKNSRDLGGQIKLREIPTAALVLGVNVTDHDLTFGS
LTEALQNNVTPYVVSLQAKDCPDMKHFLQKLISQLMDCCVDIKSKEEESVHVTQRKTHYS
MDSLSSWYMTVTQKTDPKMLSKKRTTSSQWQSPPVVVILKDMESFATKVLQDFIIISSQH
LHEFPLILIFGIATSPIIIHRLLPHAVSSLLCIELFQSLSCKEHLTTVLDKLLLTTQFPF
KINEKVLQVLTNIFLYHDFSVQNFIKGLQLSLLEHFYSQPLSVLCCNLPEAKRRINFLSN
NQCENIRRLPSFRRYVEKQASEKQVALLTNERYLKEETQLLLENLHVYHMNYFLVLRCLH
KFTSSLPKYPLGRQIRELYCTCLEKNIWDSEEYASVLQLLRMLAKDELMTILEKCFKVFK
SYCENHLGSTAKRIEEFLAQFQSLDAETKEEEDASGSQPKGLQKTDLYHLQKSLLEMKEL
RRSKKQTKFEVLRENVVNFIDCLVREYLLPPETQPLHEVVYFSAAHALREHLNAAPRIAL
HTALNNPYYYLKNEALKSEEGCIPNIAPDICIAYKLHLECSRLINLVDWSEAFATVVTAA
EKMDANSATSEEMNEIIHARFIRAVSELELLGFIKPTKQKTDHVARLTWGGC
Sequence of entity 6 (D), FASTA
>8S0C_6 Origin recognition complex subunit 4 (chains D)
MSSRKSKSNSLIHTECLSQVQRILRERFCRQSPHSNLFGVQVQYKHLSELLKRTALHGES
NSVLIIGPRGSGKTMLINHALKELMEIEEVSENVLQVHLNGLLQINDKIALKEITRQLNL
ENVVGDKVFGSFAENLSFLLEALKKGDRTSSCPVIFILDEFDLFAHHKNQTLLYNLFDIS
QSAQTPIAVIGLTCRLDILELLEKRVKSRFSHRQIHLMNSFGFPQYVKIFKEQLSLPAEF
PDKVFAEKWNENVQYLSEDRSVQEVLQKHFNISKNLRSLHMLLMLALNRVTASHPFMTAV
DLMEASQLCSMDSKANIVHGLSVLEICLIIAMKHLNDIYEEEPFNFQMVYNEFQKFVQRK
AHSVYNFEKPVVMKAFEHLQQLELIKPMERTSGNSQREYQLMKLLLDNTQIMNALQKYPN
CPTDVRQWATSSLSWL
Sequence of entity 7 (E), FASTA
>8S0C_7 Origin recognition complex subunit 5 (chains E)
MPHLENVVLCRESQVSILQSLFGERHHFSFPSIFIYGHTASGKTYVTQTLLKTLELPHVF
VNCVECFTLRLLLEQILNKLNHLSSSEDGCSTEITCETFNDFVRLFKQVTTAENLKDQTV
YIVLDKAEYLRDMEANLLPGFLRLQELADRNVTVLFLSEIVWEKFRPNTGCFEPFVLYFP
DYSIGNLQKILSHDHPPEYSADFYAAYINILLGVFYTVCRDLKELRHLAVLNFPKYCEPV
VKGEASERDTRKLWRNIEPHLKKAMQTVYLREISSSQWEKLQKDDTDPGQLKGLSAHTHV
ELPYYSKFILIAAYLASYNPARTDKRFFLKHHGKIKKTNFLKKHEKTSNHLLGPKPFPLD
RLLAILYSIVDSRVAPTANIFSQITSLVTLQLLTLVGHDDQLDGPKYKCTVSLDFIRAIA
RTVNFDIIKYLYDFL
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| AGS | Phosphothiophosphoric acid-adenylate ester | C10 H16 N5 O12 P3 S | 2 |
| MG | Magnesium ion | Mg | 2 |
Primary citation
MCM double hexamer loading visualized with human proteins. Weissmann, F., Greiwe, J.F., Puhringer, T. et al. Nature (2024) 636:499-508. DOI 10.1038/s41586-024-08263-6 · PubMed
Other PDB entries of the same protein (UniProt Q13415 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 6P3W 2.54 Å, Crystal structure of the Cyclin A-CDK2-ORC1 complex
- 11RL 2.8 Å, CryoEM structure of the human origin recognition complex with DNA and CDC6 protein
- 7JPO 3.2 Å, ORC-O1AAA: Human Origin Recognition Complex (ORC) with dynamic/unresolved ORC2 WH
- 5UJ7 3.39 Å, Structure of the active form of human Origin Recognition Complex ATPase motor module,…
- 8S0D 3.6 Å, H. sapiens MCM bound to double stranded DNA and ORC1-6
- 7JPP 3.7 Å, ORC-O2WH: Human Origin Recognition Complex (ORC) with dynamic/unresolved ORC1 AAA+ domain
- 8S0E 3.8 Å, H. sapiens OCCM bound to double stranded DNA
- 7JPR 4.0 Å, ORC-OPEN: Human Origin Recognition Complex (ORC) in an open conformation
- 8S0F 4.1 Å, H. sapiens OC1M bound to double stranded DNA
- 7JPS 4.4 Å, ORC-DNA: Human Origin Recognition Complex (ORC) with DNA bound in the core
- 7CTF 4.8 Å, Human origin recognition complex 1-5 State II
- 7CTG 5.0 Å, Human Origin Recognition Complex, ORC1-5 State I
Browse structure collections
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