8SZI: PDB entry 8SZI
Cryo-EM structure of PAM-free human calcium-sensing receptor CaSR-Gi complex in lipid nanodiscs. Determined by electron microscopy at 3.5 Å resolution. Released 7 Feb 2024.
- Method
- Electron microscopy
- Resolution
- 3.5 Å
- Organism
- Homo sapiens
- Chains
- 5
- Atoms
- 17,868
- Mol. weight
- 304.84 kDa
- Ligands
- CA, NAG, PCW, CLR
- Released
- 7 Feb 2024
Explore 8SZI in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8SZI contains 95 α-helices and 106 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 42 helices, 33 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 26-28 | 3 | 11 |
| β-strand | 32-38 | 7 | 11 |
| β-strand | 41 | 1 | 12 |
| β-strand | 44 | 1 | 13 |
| α-helix | 48-50 | 3 | |
| β-strand | 60 | 1 | 13 |
| β-strand | 63 | 1 | 12 |
| α-helix | 65-82 | 18 | |
| β-strand | 93-99 | 7 | 11 |
| α-helix | 104-114 | 11 | |
| α-helix | 136-137 | 2 | |
| β-strand | 138-142 | 5 | 11 |
| α-helix | 147-157 | 11 | |
| β-strand | 164-166 | 3 | 11 |
| α-helix | 172-175 | 4 | |
| β-strand | 183-185 | 3 | 11 |
| α-helix | 191-203 | 13 | |
| β-strand | 208-214 | 7 | 14 |
| α-helix | 219-232 | 14 | |
| β-strand | 236-243 | 8 | 14 |
| α-helix | 249-260 | 12 | |
| β-strand | 266-270 | 5 | 14 |
| α-helix | 273-285 | 13 | |
| β-strand | 292-295 | 4 | 14 |
| α-helix | 308-310 | 3 | |
| α-helix | 311-314 | 4 | |
| β-strand | 318-321 | 4 | 14 |
| α-helix | 322-324 | 3 | |
| β-strand | 325 | 1 | 15 |
| α-helix | 330-334 | 5 | |
| α-helix | 348-356 | 9 | |
| β-strand | 359 | 1 | 16 |
| α-helix | 392-393 | 2 | |
| β-strand | 394 | 1 | 16 |
| α-helix | 395 | 1 | |
| β-strand | 414 | 1 | 15 |
| α-helix | 416-435 | 20 | |
| α-helix | 448-451 | 4 | |
| α-helix | 457-465 | 9 | |
| β-strand | 468-470 | 3 | 17 |
| β-strand | 476-478 | 3 | 17 |
| β-strand | 489-496 | 8 | 14 |
| β-strand | 503-511 | 9 | 14 |
| β-strand | 521-523 | 3 | 14 |
| α-helix | 525-527 | 3 | |
| α-helix | 544-546 | 3 | |
| β-strand | 550-554 | 5 | 18 |
| α-helix | 555 | 1 | |
| β-strand | 563-567 | 5 | 18 |
| α-helix | 568-569 | 2 | |
| β-strand | 572-573 | 2 | 19 |
| β-strand | 583-584 | 2 | 19 |
| α-helix | 585-586 | 2 | |
| β-strand | 589-591 | 3 | 20 |
| β-strand | 598-600 | 3 | 20 |
| β-strand | 602-604 | 3 | 21 |
| α-helix | 611-636 | 26 | |
| α-helix | 641-645 | 5 | |
| α-helix | 648-663 | 16 | |
| α-helix | 664-668 | 5 | |
| α-helix | 674-677 | 4 | |
| α-helix | 680-707 | 28 | |
| α-helix | 721-745 | 25 | |
| β-strand | 749-753 | 5 | 21 |
| β-strand | 760-766 | 7 | 21 |
| α-helix | 771-793 | 23 | |
| α-helix | 798 | 1 | |
| α-helix | 807-820 | 14 | |
| α-helix | 822-827 | 6 | |
| α-helix | 832-849 | 18 | |
| α-helix | 850-854 | 5 | |
| α-helix | 855-861 | 7 | |
| α-helix | 864-866 | 3 | |
| α-helix | 869-877 | 9 | |
| α-helix | 884-886 | 3 | |
Chain B: 35 helices, 35 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 26-28 | 3 | 22 |
| β-strand | 32-38 | 7 | 22 |
| β-strand | 41 | 1 | 23 |
| β-strand | 44 | 1 | 24 |
| α-helix | 57-59 | 3 | |
| β-strand | 60 | 1 | 24 |
| β-strand | 63 | 1 | 23 |
| α-helix | 67-83 | 17 | |
| β-strand | 93-99 | 7 | 22 |
| α-helix | 104-111 | 8 | |
| α-helix | 136-137 | 2 | |
| β-strand | 138-142 | 5 | 22 |
| α-helix | 147-157 | 11 | |
| β-strand | 164-166 | 3 | 22 |
| α-helix | 172-175 | 4 | |
| β-strand | 183-185 | 3 | 22 |
| α-helix | 191-203 | 13 | |
| β-strand | 208-214 | 7 | 25 |
| α-helix | 219-232 | 14 | |
| β-strand | 236-243 | 8 | 25 |
| α-helix | 249-259 | 11 | |
| β-strand | 266-270 | 5 | 25 |
| α-helix | 273-286 | 14 | |
| β-strand | 292-295 | 4 | 25 |
| α-helix | 308-310 | 3 | |
| α-helix | 311-314 | 4 | |
| β-strand | 318-321 | 4 | 25 |
| β-strand | 325 | 1 | 26 |
| α-helix | 330-334 | 5 | |
| α-helix | 348-356 | 9 | |
| β-strand | 359 | 1 | 27 |
| β-strand | 394 | 1 | 27 |
| α-helix | 401-403 | 3 | |
| β-strand | 414 | 1 | 26 |
| α-helix | 417-435 | 19 | |
| α-helix | 445-447 | 3 | |
| α-helix | 452-454 | 3 | |
| α-helix | 457-465 | 9 | |
| β-strand | 468-470 | 3 | 28 |
| β-strand | 476-478 | 3 | 28 |
| β-strand | 489-496 | 8 | 25 |
| β-strand | 503-511 | 9 | 25 |
| β-strand | 521-523 | 3 | 25 |
| α-helix | 525-527 | 3 | |
| β-strand | 530 | 1 | 29 |
| β-strand | 534 | 1 | 29 |
| α-helix | 544-547 | 4 | |
| β-strand | 550-554 | 5 | 30 |
| β-strand | 563-567 | 5 | 30 |
| β-strand | 572-573 | 2 | 31 |
| β-strand | 583-584 | 2 | 31 |
| α-helix | 585 | 1 | |
| β-strand | 589-591 | 3 | 32 |
| β-strand | 598-600 | 3 | 32 |
| α-helix | 601 | 1 | |
| β-strand | 602-604 | 3 | 33 |
| α-helix | 611-636 | 26 | |
| α-helix | 641-645 | 5 | |
| α-helix | 648-664 | 17 | |
| α-helix | 665-667 | 3 | |
| α-helix | 674-697 | 24 | |
| α-helix | 703-706 | 4 | |
| α-helix | 723-745 | 23 | |
| β-strand | 749-753 | 5 | 33 |
| β-strand | 760-766 | 7 | 33 |
| α-helix | 770-793 | 24 | |
| α-helix | 799-827 | 29 | |
| α-helix | 831-833 | 3 | |
| α-helix | 835-860 | 26 | |
| α-helix | 871-875 | 5 | |
Chain C: 9 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 8-30 | 23 | |
| β-strand | 34-38 | 5 | 1 |
| α-helix | 46-53 | 8 | |
| β-strand | 185-190 | 6 | 1 |
| β-strand | 195-200 | 6 | 1 |
| α-helix | 208-212 | 5 | |
| β-strand | 220-226 | 7 | 1 |
| α-helix | 227-231 | 5 | |
| α-helix | 242-254 | 13 | |
| β-strand | 263-269 | 7 | 1 |
| α-helix | 271-278 | 8 | |
| α-helix | 283-285 | 3 | |
| α-helix | 296-308 | 13 | |
| β-strand | 319-323 | 5 | 1 |
| α-helix | 330-351 | 22 | |
Chain D: 5 helices, 32 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-24 | 21 | |
| α-helix | 30-33 | 4 | |
| α-helix | 38-39 | 2 | |
| β-strand | 47-51 | 5 | 2 |
| β-strand | 58-63 | 6 | 3 |
| β-strand | 70-74 | 5 | 3 |
| β-strand | 79-81 | 3 | 3 |
| β-strand | 91-92 | 2 | 3 |
| β-strand | 100 | 1 | 4 |
| β-strand | 104-105 | 2 | 5 |
| β-strand | 111-113 | 3 | 5 |
| β-strand | 116 | 1 | 4 |
| β-strand | 120-125 | 6 | 5 |
| β-strand | 129 | 1 | 6 |
| β-strand | 132 | 1 | 6 |
| β-strand | 135-140 | 6 | 5 |
| β-strand | 146-151 | 6 | 7 |
| β-strand | 156-161 | 6 | 7 |
| β-strand | 165-170 | 6 | 7 |
| β-strand | 178-180 | 3 | 7 |
| β-strand | 187-192 | 6 | 8 |
| β-strand | 198-203 | 6 | 8 |
| β-strand | 208-212 | 5 | 8 |
| β-strand | 220-222 | 3 | 8 |
| β-strand | 229-234 | 6 | 9 |
| β-strand | 240-245 | 6 | 9 |
| β-strand | 250-254 | 5 | 9 |
| β-strand | 259-264 | 6 | 9 |
| β-strand | 273-278 | 6 | 10 |
| α-helix | 279 | 1 | |
| α-helix | 280-282 | 3 | |
| β-strand | 284-289 | 6 | 10 |
| β-strand | 294-298 | 5 | 10 |
| β-strand | 306-308 | 3 | 10 |
| β-strand | 315-320 | 6 | 2 |
| β-strand | 327-331 | 5 | 2 |
| β-strand | 336-339 | 4 | 2 |
Chain E: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 9-23 | 15 | |
| α-helix | 30-43 | 14 | |
| α-helix | 45-47 | 3 | |
| α-helix | 53-55 | 3 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Guanine nucleotide-binding protein G(i) subunit alpha-3 | C | protein | 354 | Homo sapiens | P08754 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | D | protein | 343 | Homo sapiens | P62873 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | E | protein | 71 | Homo sapiens | P59768 (AlphaFold model) |
| Extracellular calcium-sensing receptor | A | protein | 939 | Homo sapiens | P41180 (AlphaFold model) |
| Extracellular calcium-sensing receptor | B | protein | 959 | Homo sapiens | P41180 (AlphaFold model) |
Sequence of entity 1 (C), FASTA
>8SZI_1 Guanine nucleotide-binding protein G(i) subunit alpha-3 (chains C)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKNTIVKQMKIIHEDG
YSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMT
PELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVK
TTGIVETHFTFKDLYFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAA
AYIQCQFEDLNRRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKECGLY
Sequence of entity 2 (D), FASTA
>8SZI_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains D)
GSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAK
IYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGL
DNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQT
TTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPN
GNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVW
DALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 3 (E), FASTA
>8SZI_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains E)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 4 (A), FASTA
>8SZI_4 Extracellular calcium-sensing receptor (chains A)
DYKDDDDKAAAYGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGF
RWLQAMIFAIEEINSSPALLPNLTLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEF
CNCSEHIPSTIAVVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQFKSFLRTIP
NDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKFREEAEERDICIDFSELISQYSD
EEEIQHVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGKIWLASEAWASSSLIAMP
QYFHVVGGTIGFALKAGQIPGFREFLKKVHPRKSVHNGFAKEFWEETFNCHLQEGAKGPL
PVDTFLRGHEESGDRFSNSSTAFRPLCTGDENISSVETPYIDYTHLRISYNVYLAVYSIA
HALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECGDLVGN
YSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEEKILWSGFSREVPFSNCSRDCLA
GTRKGIIEGEPTCCFECVECPDGEYSDETDASACNKCPDDFWSNENHTSCIAKEIEFLSW
TEPFGIALTLFAVLGIFLTAFVLGVFIKFRNTPIVKATNRELSYLLLFSLLCCFSSSLFF
IGEPQDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSFHRKWWGLNLQFLLV
FLCTFMQIVICVIWLYTAPPSSYRNQELEDEIIFITCHEGSLMALGFLIGYTCLLAAICF
FFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIAILAASFGL
LACIFFNKIYIILFKPSRNTIEEVRCSTAAHAFKVAARATLRRSNVTSTSVTSVNQASTS
RLEGLQSENHRLRMKITELDKDLEEVTMQLQDTPEKKTN
Sequence of entity 5 (B), FASTA
>8SZI_5 Extracellular calcium-sensing receptor (chains B)
WSHPQFEKGGGSGGGSGGSAWSHPQFEKGSAAAYGPDQRAQKKGDIILGGLFPIHFGVAA
KDQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINSSPALLPNLTLGYRIFDTCNTVSK
ALEATLSFVAQNKIDSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLFYIPQVS
YASSSRLLSNKNQFKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKF
REEAEERDICIDFSELISQYSDEEEIQHVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRR
NITGKIWLASEAWASSSLIAMPQYFHVVGGTIGFALKAGQIPGFREFLKKVHPRKSVHNG
FAKEFWEETFNCHLQEGAKGPLPVDTFLRGHEESGDRFSNSSTAFRPLCTGDENISSVET
PYIDYTHLRISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRH
LNFTNNMGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEE
KILWSGFSREVPFSNCSRDCLAGTRKGIIEGEPTCCFECVECPDGEYSDETDASACNKCP
DDFWSNENHTSCIAKEIEFLSWTEPFGIALTLFAVLGIFLTAFVLGVFIKFRNTPIVKAT
NRELSYLLLFSLLCCFSSSLFFIGEPQDWTCRLRQPAFGISFVLCISCILVKTNRVLLVF
EAKIPTSFHRKWWGLNLQFLLVFLCTFMQIVICVIWLYTAPPSSYRNQELEDEIIFITCH
EGSLMALGFLIGYTCLLAAICFFFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYA
STYGKFVSAVEVIAILAASFGLLACIFFNKIYIILFKPSRNTIEEVRCSTAAHAFKVAAR
ATLRRSNVTGSSTNNNEEEKSRLLEKENRELEKIIAEKEERVSELRHQLQSRQQLKKTN
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| CA | Calcium ion | Ca | 4 |
| NAG | 2-acetamido-2-deoxy-beta-D-glucopyranose | C8 H15 N O6 | 3 |
| PCW | 1,2-dioleoyl-sn-glycero-3-phosphocholine | C44 H85 N O8 P | 2 |
| CLR | Cholesterol | C27 H46 O | 1 |
| PO4 | Phosphate ion | O4 P | 2 |
| TRP | Tryptophan | C11 H12 N2 O2 | 3 |
Primary citation
Allosteric modulation and G-protein selectivity of the Ca 2+ -sensing receptor. He, F., Wu, C.G., Gao, Y. et al. Nature (2024) 626:1141-1148. DOI 10.1038/s41586-024-07055-2 · PubMed
Other PDB entries of the same protein (UniProt P08754 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 2ODE 1.9 Å, Crystal structure of the heterodimeric complex of human RGS8 and activated Gi alpha 3
- 9YCX 2.1 Å, Neurotensin Receptor 1 (NTSR1) bound to Octotensin in complex with Gi3 in the…
- 9YCY 2.2 Å, Neurotensin Receptor 1 (NTSR1) bound to Octotensin in complex with Gi3 in the Canonical…
- 8IKL 2.33 Å, Cryo-EM structure of the CD97-G13 complex
- 8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
- 7T10 2.5 Å, CryoEM structure of somatostatin receptor 2 in complex with somatostatin-14 and Gi3
- 9VJ6 2.62 Å, Cryo-EM structure of 5-HT1AR-Gi3 in complex with F-15599
- 7T11 2.7 Å, CryoEM structure of somatostatin receptor 2 in complex with Octreotide and Gi3.
- 9VJF 2.7 Å, Cryo-EM structure of 5-HT1AR-Gi3 in complex with buspirone
- 2IHB 2.71 Å, Crystal structure of the heterodimeric complex of human RGS10 and activated Gi alpha 3
- 2V4Z 2.8 Å, The crystal structure of the human G-protein subunit alpha (GNAI3) in complex with an…
- 7KH0 2.8 Å, Cryo-EM structure of the human arginine vasopressin AVP-vasopressin receptor V2R-Gs…
Browse structure collections
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