8W7M: Yeast replisome in state V
Yeast replisome in state V. Determined by electron microscopy at 4.12 Å resolution. Released 6 Dec 2023.
- Method
- Electron microscopy
- Resolution
- 4.12 Å
- Organisms
- Saccharomyces cerevisiae, Saccharomyces cerevisiae S288C
- Chains
- 16
- Atoms
- 51,196
- Mol. weight
- 1202.5 kDa
- Ligands
- AGS, ZN, MG, ADP
- Released
- 6 Dec 2023
Explore 8W7M in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8W7M contains 280 α-helices and 283 β-strands across 15 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain 2: 30 helices, 24 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 183-187 | 5 | |
| α-helix | 194-199 | 6 | |
| α-helix | 201-217 | 17 | |
| α-helix | 226-237 | 12 | |
| β-strand | 241-243 | 3 | 1 |
| α-helix | 246-251 | 6 | |
| α-helix | 254-262 | 9 | |
| α-helix | 264-282 | 19 | |
| α-helix | 286-288 | 3 | |
| β-strand | 294-296 | 3 | 1 |
| β-strand | 303 | 1 | 2 |
| β-strand | 317-327 | 11 | 2 |
| β-strand | 331-341 | 11 | 2 |
| β-strand | 348-352 | 5 | 2 |
| α-helix | 353 | 1 | |
| β-strand | 364 | 1 | 3 |
| β-strand | 369 | 1 | 3 |
| β-strand | 373-391 | 19 | 2 |
| α-helix | 398 | 1 | |
| α-helix | 401-403 | 3 | |
| β-strand | 405-411 | 7 | 2 |
| β-strand | 423-433 | 11 | 2 |
| α-helix | 436-442 | 7 | |
| β-strand | 446 | 1 | 4 |
| β-strand | 448-457 | 10 | 2 |
| α-helix | 478-487 | 10 | |
| α-helix | 493-500 | 8 | |
| α-helix | 509-520 | 12 | |
| β-strand | 525 | 1 | 5 |
| β-strand | 533 | 1 | 5 |
| β-strand | 539-543 | 5 | 6 |
| α-helix | 549-559 | 11 | |
| β-strand | 563-565 | 3 | 6 |
| α-helix | 573-577 | 5 | |
| β-strand | 578-581 | 4 | 7 |
| β-strand | 590-593 | 4 | 7 |
| α-helix | 595-598 | 4 | |
| β-strand | 603-605 | 3 | 6 |
| α-helix | 615-625 | 11 | |
| β-strand | 629-631 | 3 | 8 |
| α-helix | 633-635 | 3 | |
| β-strand | 638-640 | 3 | 8 |
| β-strand | 646-648 | 3 | 6 |
| α-helix | 652-654 | 3 | |
| α-helix | 671-676 | 6 | |
| β-strand | 679-683 | 5 | 6 |
| α-helix | 689-706 | 18 | |
| α-helix | 748-751 | 4 | |
| α-helix | 760-773 | 14 | |
| α-helix | 780-799 | 20 | |
| α-helix | 807-823 | 17 | |
| α-helix | 831-846 | 16 | |
| α-helix | 850-861 | 12 | |
| α-helix | 862-866 | 5 | |
Chain 3: 28 helices, 32 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 19-34 | 16 | |
| α-helix | 36-53 | 18 | |
| β-strand | 95-99 | 5 | 9 |
| α-helix | 100-106 | 7 | |
| α-helix | 108-116 | 9 | |
| α-helix | 118-136 | 19 | |
| α-helix | 144-149 | 6 | |
| β-strand | 154-158 | 5 | 9 |
| α-helix | 162-164 | 3 | |
| β-strand | 165-166 | 2 | 10 |
| α-helix | 173-175 | 3 | |
| β-strand | 178-187 | 10 | 10 |
| α-helix | 190-192 | 3 | |
| β-strand | 194-196 | 3 | 11 |
| β-strand | 198 | 1 | 12 |
| β-strand | 201-203 | 3 | 13 |
| β-strand | 208-209 | 2 | 13 |
| β-strand | 210 | 1 | 14 |
| β-strand | 213 | 1 | 12 |
| α-helix | 225-227 | 3 | |
| β-strand | 234 | 1 | 15 |
| α-helix | 239 | 1 | |
| β-strand | 240 | 1 | 15 |
| β-strand | 241-243 | 3 | 13 |
| β-strand | 250-252 | 3 | 11 |
| β-strand | 253 | 1 | 16 |
| β-strand | 256-259 | 4 | 10 |
| α-helix | 265-266 | 2 | |
| α-helix | 270-272 | 3 | |
| β-strand | 273-276 | 4 | 10 |
| β-strand | 279 | 1 | 16 |
| α-helix | 280-282 | 3 | |
| β-strand | 291-301 | 11 | 10 |
| β-strand | 317 | 1 | 17 |
| β-strand | 318-328 | 11 | 10 |
| α-helix | 344-355 | 12 | |
| α-helix | 359-366 | 8 | |
| α-helix | 375-386 | 12 | |
| β-strand | 391-392 | 2 | 18 |
| β-strand | 398-399 | 2 | 18 |
| β-strand | 405-408 | 4 | 19 |
| α-helix | 415-425 | 11 | |
| β-strand | 431-433 | 3 | 19 |
| α-helix | 439-442 | 4 | |
| β-strand | 444-447 | 4 | 20 |
| β-strand | 456-459 | 4 | 20 |
| β-strand | 469-473 | 5 | 19 |
| α-helix | 475-477 | 3 | |
| α-helix | 480-492 | 13 | |
| β-strand | 494-499 | 6 | 21 |
| β-strand | 502-507 | 6 | 21 |
| β-strand | 511-516 | 6 | 19 |
| α-helix | 529-532 | 4 | |
| α-helix | 537-542 | 6 | |
| β-strand | 545-548 | 4 | 19 |
| α-helix | 555-569 | 15 | |
| α-helix | 653-665 | 13 | |
| α-helix | 673-688 | 16 | |
| α-helix | 699-715 | 17 | |
| α-helix | 723-737 | 15 | |
Chain 4: 29 helices, 32 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 179-180 | 2 | 22 |
| β-strand | 185-186 | 2 | 22 |
| α-helix | 187-198 | 12 | |
| α-helix | 203-209 | 7 | |
| α-helix | 213-215 | 3 | |
| α-helix | 218-222 | 5 | |
| α-helix | 225-236 | 12 | |
| β-strand | 240-244 | 5 | 23 |
| α-helix | 245-250 | 6 | |
| α-helix | 252-263 | 12 | |
| α-helix | 265-286 | 22 | |
| α-helix | 293-298 | 6 | |
| β-strand | 302-306 | 5 | 23 |
| α-helix | 314-316 | 3 | |
| α-helix | 319-321 | 3 | |
| β-strand | 325 | 1 | 24 |
| β-strand | 328-335 | 8 | 24 |
| α-helix | 336-338 | 3 | |
| β-strand | 339-349 | 11 | 24 |
| β-strand | 355-359 | 5 | 24 |
| β-strand | 361 | 1 | 25 |
| β-strand | 364 | 1 | 25 |
| β-strand | 382-384 | 3 | 24 |
| β-strand | 390-401 | 12 | 24 |
| α-helix | 402 | 1 | |
| α-helix | 411-413 | 3 | |
| β-strand | 414-420 | 7 | 24 |
| α-helix | 421-423 | 3 | |
| β-strand | 432-442 | 11 | 24 |
| β-strand | 445 | 1 | 26 |
| β-strand | 453-454 | 2 | 26 |
| β-strand | 456 | 1 | 27 |
| β-strand | 458-468 | 11 | 24 |
| α-helix | 503-514 | 12 | |
| α-helix | 518-526 | 9 | |
| α-helix | 534-545 | 12 | |
| β-strand | 550-551 | 2 | 28 |
| β-strand | 557-558 | 2 | 28 |
| β-strand | 564-567 | 4 | 29 |
| α-helix | 574-584 | 11 | |
| β-strand | 588-592 | 5 | 29 |
| β-strand | 596 | 1 | 30 |
| α-helix | 598-602 | 5 | |
| β-strand | 603-606 | 4 | 31 |
| β-strand | 615-618 | 4 | 31 |
| α-helix | 620-624 | 5 | |
| β-strand | 628-632 | 5 | 29 |
| α-helix | 639-651 | 13 | |
| β-strand | 653-658 | 6 | 32 |
| β-strand | 661-666 | 6 | 32 |
| β-strand | 670-675 | 6 | 29 |
| α-helix | 688-692 | 5 | |
| α-helix | 696-701 | 6 | |
| β-strand | 704-706 | 3 | 29 |
| α-helix | 714-729 | 16 | |
| α-helix | 744-757 | 14 | |
| α-helix | 764-779 | 16 | |
| α-helix | 795-811 | 17 | |
| α-helix | 819-832 | 14 | |
| β-strand | 838 | 1 | 33 |
| β-strand | 845 | 1 | 33 |
Chain 5: 23 helices, 20 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 21-37 | 17 | |
| β-strand | 40 | 1 | 34 |
| β-strand | 43 | 1 | 34 |
| α-helix | 45-55 | 11 | |
| β-strand | 60-64 | 5 | 35 |
| α-helix | 65-71 | 7 | |
| α-helix | 73-81 | 9 | |
| α-helix | 83-102 | 20 | |
| α-helix | 132-135 | 4 | |
| β-strand | 136-140 | 5 | 35 |
| α-helix | 148-150 | 3 | |
| α-helix | 153-155 | 3 | |
| β-strand | 159-169 | 11 | 17 |
| β-strand | 173-183 | 11 | 17 |
| β-strand | 189-193 | 5 | 17 |
| β-strand | 241-243 | 3 | 17 |
| β-strand | 249-259 | 11 | 17 |
| α-helix | 266 | 1 | |
| α-helix | 270-272 | 3 | |
| β-strand | 273-279 | 7 | 17 |
| α-helix | 280-282 | 3 | |
| β-strand | 290-301 | 12 | 17 |
| β-strand | 323 | 1 | 2 |
| β-strand | 325-335 | 11 | 17 |
| α-helix | 351-362 | 12 | |
| α-helix | 366-373 | 8 | |
| α-helix | 382-393 | 12 | |
| β-strand | 398-399 | 2 | 36 |
| β-strand | 405-406 | 2 | 36 |
| β-strand | 411-414 | 4 | 37 |
| α-helix | 420-432 | 13 | |
| β-strand | 437-438 | 2 | 37 |
| α-helix | 468-471 | 4 | |
| β-strand | 476-479 | 4 | 37 |
| α-helix | 489-498 | 10 | |
| β-strand | 518-522 | 5 | 37 |
| α-helix | 545-549 | 5 | |
| β-strand | 552-553 | 2 | 37 |
| α-helix | 562-576 | 15 | |
| α-helix | 596-609 | 14 | |
| α-helix | 616-635 | 20 | |
| α-helix | 650-667 | 18 | |
| α-helix | 674-692 | 19 | |
Chain 6: 29 helices, 27 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 106-120 | 15 | |
| α-helix | 135-146 | 12 | |
| β-strand | 150-154 | 5 | 38 |
| α-helix | 155-160 | 6 | |
| α-helix | 161-164 | 4 | |
| α-helix | 165-172 | 8 | |
| α-helix | 174-192 | 19 | |
| α-helix | 194-197 | 4 | |
| β-strand | 198 | 1 | 39 |
| β-strand | 260 | 1 | 39 |
| α-helix | 261-262 | 2 | |
| β-strand | 264-268 | 5 | 38 |
| α-helix | 281-283 | 3 | |
| β-strand | 284 | 1 | 4 |
| β-strand | 287-297 | 11 | 4 |
| β-strand | 301-311 | 11 | 4 |
| β-strand | 317-322 | 6 | 4 |
| α-helix | 327-328 | 2 | |
| β-strand | 344-346 | 3 | 4 |
| β-strand | 352-362 | 11 | 4 |
| β-strand | 376-382 | 7 | 4 |
| α-helix | 383-385 | 3 | |
| β-strand | 394-405 | 12 | 4 |
| α-helix | 407-410 | 4 | |
| β-strand | 450-461 | 12 | 4 |
| α-helix | 501-507 | 7 | |
| α-helix | 510-521 | 12 | |
| α-helix | 525-532 | 8 | |
| α-helix | 541-552 | 12 | |
| β-strand | 557-558 | 2 | 40 |
| β-strand | 564-565 | 2 | 40 |
| β-strand | 571-575 | 5 | 41 |
| α-helix | 581-591 | 11 | |
| β-strand | 596-599 | 4 | 41 |
| α-helix | 605-608 | 4 | |
| β-strand | 610-614 | 5 | 42 |
| β-strand | 621-625 | 5 | 42 |
| α-helix | 627-631 | 5 | |
| β-strand | 635-639 | 5 | 41 |
| α-helix | 646-658 | 13 | |
| β-strand | 661-663 | 3 | 43 |
| β-strand | 670-672 | 3 | 43 |
| β-strand | 677-682 | 6 | 41 |
| α-helix | 685-687 | 3 | |
| α-helix | 695-698 | 4 | |
| β-strand | 699 | 1 | 44 |
| α-helix | 703-707 | 5 | |
| β-strand | 711-715 | 5 | 41 |
| α-helix | 721-736 | 16 | |
| α-helix | 738-741 | 4 | |
| α-helix | 748-760 | 13 | |
| β-strand | 764 | 1 | 45 |
| α-helix | 767-784 | 18 | |
| α-helix | 797-814 | 18 | |
| β-strand | 818 | 1 | 45 |
| α-helix | 821-835 | 15 | |
Chain 7: 33 helices, 26 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 7 | 1 | 14 |
| α-helix | 14-27 | 14 | |
| α-helix | 62-71 | 10 | |
| β-strand | 77-81 | 5 | 46 |
| α-helix | 82-95 | 14 | |
| α-helix | 102-108 | 7 | |
| α-helix | 110-123 | 14 | |
| α-helix | 125-128 | 4 | |
| α-helix | 138-150 | 13 | |
| α-helix | 194-197 | 4 | |
| β-strand | 201-205 | 5 | 46 |
| α-helix | 206-208 | 3 | |
| α-helix | 232-234 | 3 | |
| β-strand | 237-248 | 12 | 27 |
| α-helix | 249-251 | 3 | |
| β-strand | 252-254 | 3 | 27 |
| β-strand | 257-261 | 5 | 47 |
| β-strand | 268-272 | 5 | 47 |
| β-strand | 277-278 | 2 | 26 |
| α-helix | 287-291 | 5 | |
| α-helix | 294-296 | 3 | |
| β-strand | 299-300 | 2 | 47 |
| β-strand | 308-316 | 9 | 27 |
| α-helix | 323 | 1 | |
| β-strand | 330-336 | 7 | 27 |
| α-helix | 337-339 | 3 | |
| β-strand | 348-358 | 11 | 27 |
| α-helix | 362-368 | 7 | |
| β-strand | 371 | 1 | 11 |
| β-strand | 373-383 | 11 | 27 |
| α-helix | 395-407 | 13 | |
| α-helix | 410-417 | 8 | |
| α-helix | 426-437 | 12 | |
| α-helix | 439-441 | 3 | |
| β-strand | 442-443 | 2 | 48 |
| β-strand | 449-450 | 2 | 48 |
| β-strand | 455-459 | 5 | 49 |
| α-helix | 466-476 | 11 | |
| β-strand | 481-484 | 4 | 49 |
| α-helix | 490-494 | 5 | |
| β-strand | 495-498 | 4 | 50 |
| β-strand | 507-510 | 4 | 50 |
| α-helix | 512-515 | 4 | |
| β-strand | 520-524 | 5 | 49 |
| α-helix | 531-542 | 12 | |
| β-strand | 546-548 | 3 | 51 |
| β-strand | 549 | 1 | 30 |
| β-strand | 555-557 | 3 | 51 |
| β-strand | 562-567 | 6 | 49 |
| α-helix | 580-584 | 5 | |
| α-helix | 588-593 | 6 | |
| β-strand | 596-598 | 3 | 49 |
| α-helix | 606-621 | 16 | |
| α-helix | 625-626 | 2 | |
| α-helix | 635-646 | 12 | |
| α-helix | 654-673 | 20 | |
| α-helix | 677-679 | 3 | |
| α-helix | 686-703 | 18 | |
| α-helix | 710-729 | 20 | |
Chain A: 9 helices, 5 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-22 | 21 | |
| α-helix | 28-32 | 5 | |
| α-helix | 34-60 | 27 | |
| α-helix | 64-68 | 5 | |
| α-helix | 69-100 | 32 | |
| α-helix | 101-105 | 5 | |
| α-helix | 120-122 | 3 | |
| α-helix | 125-142 | 18 | |
| β-strand | 162-167 | 6 | 52 |
| β-strand | 172-175 | 4 | 53 |
| β-strand | 180-183 | 4 | 53 |
| β-strand | 188-192 | 5 | 52 |
| α-helix | 193-201 | 9 | |
| β-strand | 205-206 | 2 | 52 |
Chain B: 10 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 5-7 | 3 | |
| α-helix | 13-21 | 9 | |
| β-strand | 24-29 | 6 | 54 |
| α-helix | 60-62 | 3 | |
| β-strand | 69-72 | 4 | 54 |
| α-helix | 73-81 | 9 | |
| β-strand | 85-87 | 3 | 54 |
| α-helix | 88-90 | 3 | |
| α-helix | 95-107 | 13 | |
| α-helix | 112-113 | 2 | |
| α-helix | 119-129 | 11 | |
| α-helix | 138-160 | 23 | |
| β-strand | 168 | 1 | 55 |
| α-helix | 175-199 | 25 | |
7 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| DNA replication licensing factor MCM2 | 2 | protein | 868 | Saccharomyces cerevisiae | P29469 (AlphaFold model) |
| DNA replication licensing factor MCM3 | 3 | protein | 971 | Saccharomyces cerevisiae S288C | P24279 (AlphaFold model) |
| DNA replication licensing factor MCM4 | 4 | protein | 933 | Saccharomyces cerevisiae S288C | P30665 (AlphaFold model) |
| Minichromosome maintenance protein 5 | 5 | protein | 775 | Saccharomyces cerevisiae S288C | P29496 (AlphaFold model) |
| DNA replication licensing factor MCM6 | 6 | protein | 1017 | Saccharomyces cerevisiae S288C | P53091 |
| DNA replication licensing factor MCM7 | 7 | protein | 845 | Saccharomyces cerevisiae | P38132 |
| DNA replication complex GINS protein PSF1 | A | protein | 208 | Saccharomyces cerevisiae S288C | Q12488 |
| DNA replication complex GINS protein PSF2 | B | protein | 213 | Saccharomyces cerevisiae S288C | P40359 |
| DNA replication complex GINS protein PSF3 | C | protein | 194 | Saccharomyces cerevisiae S288C | Q12146 |
| DNA replication complex GINS protein SLD5 | D | protein | 294 | Saccharomyces cerevisiae S288C | Q03406 |
| Cell division control protein 45 | E | protein | 650 | Saccharomyces cerevisiae S288C | Q08032 |
| DNA polymerase alpha-binding protein | F, G, H | protein | 927 | Saccharomyces cerevisiae S288C | Q01454 |
2 more molecules are not listed.
Sequence of entity 1 (2), FASTA
>8W7M_1 DNA replication licensing factor MCM2 (chains 2)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH
Sequence of entity 2 (3), FASTA
>8W7M_2 DNA replication licensing factor MCM3 (chains 3)
MEGSTGFDGDATTFFAPDAVFGDRVRRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQ
DDADERDLLGDDDGDDLEKEKKAASSTSLNILPHRIIISLDDLREFDRSFWSGILVEPAY
FIPPAEKALTDLADSMDDVPHPNASAVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLV
SVEGIVTKTSLVRPKLIRSVHYAAKTGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNK
LTTEYGYSTFIDHQRITVQEMPEMAPAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKS
LGAGGMNQSNSNTLIGFKTLILGNTVYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIF
DILSQSLAPSIYGHDHIKKAILLMLMGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLR
FVLNTASLAIATTGRGSSGVGLTAAVTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTD
VDRVAIHEVMEQQTVTIAKAGIHTTLNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLL
SRFDLLFVVTDDINEIRDRSISEHVLRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINP
EEHSNSGAGVENEGEDDEDHVFEKFNPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYV
QYAKERVIPQLTQEAINVIVKNYTDLRNDDNTKKSPITARTLETLIRLATAHAKVRLSKT
VNKVDAKVAANLLRFALLGEDIGNDIDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSG
SPIKSTPRRSTASSVNATPSSARRILRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQL
GLRVSPRRREHLHAPEEGSSGPLTEVGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTIST
GRLSLISGIIARLMQTEIFEEESYPVASLFERINEELPEEEKFSAQEYLAGLKIMSDRNN
LMVADDKVWRV
Sequence of entity 3 (4), FASTA
>8W7M_3 DNA replication licensing factor MCM4 (chains 4)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV
Sequence of entity 4 (5), FASTA
>8W7M_4 Minichromosome maintenance protein 5 (chains 5)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV
Sequence of entity 5 (6), FASTA
>8W7M_5 DNA replication licensing factor MCM6 (chains 6)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS
Sequence of entity 6 (7), FASTA
>8W7M_6 DNA replication licensing factor MCM7 (chains 7)
MSAALPSIQLPVDYNNLFNEITDFLVTFKQDTLSSDATRNENEDENLDAENIEQHLLEKG
PKYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRA
IDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDA
LREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLIT
VRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFM
STRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPY
TGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAP
EIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRG
VYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEV
MEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLM
LDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYV
VQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLV
RVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLS
NCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANVSAQDSDI
DLQDA
Sequence of entity 7 (A), FASTA
>8W7M_7 DNA replication complex GINS protein PSF1 (chains A)
MYGDLGNKLVLEAKRTKQLYARSNQDVNLPMYHEDIIRNILKEVSNLRKNTEYLKEQQQL
GMLDDKVAKCQYFVTLLCMERNKRCLLAYQRLRTDILDSMAWNNNGLDLMSSITFSQQDT
NNLSHQEQEYLKEYCDLITDLKSGDLVDIDLSGSLVPPSDVFIDVRVLKDAGEIQTEYGV
FNLIKDSQFFVRQSDVERLIQQGYLQKI
Sequence of entity 8 (B), FASTA
>8W7M_8 DNA replication complex GINS protein PSF2 (chains B)
MSLPAHLQQTFSPEEIQFIVENEPIKIFPRITTRQKIRGDDRGTGNHTRWQLITTDDKAL
NNMVAMRSTEVVLWIALLLKQQSKCSIVAPQWLTTKELDRKIQYEKTHPDRFSELPWNWL
VLARILFNKAKDDFHDPIHELRGKIQDLREIRQIKVLKGLKYLNESHLQLDNLSLLEINE
LRPFITEIMDKLREIHTASLTAGTENDEEEFNI
Sequence of entity 9 (C), FASTA
>8W7M_9 DNA replication complex GINS protein PSF3 (chains C)
MGYYDIDDVLADGTEFPCKFQYDIPGLGYLENNPGRPITKNTKLSLPLWLARILAIVGGD
EALVDEEPVPFVELLPPDMFSTKVMNAIKTDPVALDLHSINSHFFSLAIKWIMLFSEKEL
ANVVSELLLQRAQELNHHASSLSIDLNADSTGKNSANTNIATSTFLLKLEEMEKEIYKKS
HESYKDTKRWMFKK
Sequence of entity 10 (D), FASTA
>8W7M_10 DNA replication complex GINS protein SLD5 (chains D)
MDINIDDILAELDKETTAVDSTKITQGSSSTTHRDANTIVGSSLDLNDKTQIYVSPQQDF
SDLMKSWKNERCSPELLPYPHQLMKRLLNRISMQSQLIENISMGFLDMQNASNANPPMPN
ESKLPLLCMETELERLKFVIRSYIRCRLSKIDKFSLYLRQLNEDENSLISLTDLLSKDEI
KYHDTHSLIWLKLVNDSILKYMPEELQAINDTEGSVNMIDEPDWNKFVFIHVNGPPDGKW
NEDPLLQENEFGKPCYTVTIPDLKEEVELTIGSIYVMRYEVIRDLLRDDKVALI
Sequence of entity 11 (E), FASTA
>8W7M_11 Cell division control protein 45 (chains E)
MYYGISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFKKQLVQSQIVP
IFGYSELRRHYSQLDDNINSLLLVGFGGVIDLEAFLEIDPQEYVIDTDEKSGEQSFRRDI
YVLDAHRPWNLDNIFGSQIIQCFDDGTVDDTLGEQKEAYYKLLELDEESGDDELSGDEND
NNGGDDEATDADEVTDEDEEDEDETISNKRGNSSIGPNDLSKRKQRKKQIHEYEGVLEEY
YSQGTTVVNSISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYPLLQDEVK
RLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSLWNENGKKRLH
KMFARMGIPLSTAQETWLYMDHSIKRELGIIFDKNLDRYGLQDIIRDGFVRTLGYRGSIS
ASEFVEALTALLEVGNSTDKDSVKINNDNNDDTDGEEEEDNSAQKLTNLRKRWVSNFWLS
WDALDDRKVELLNRGIQLAQDLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDGPDLDLYR
NPLTLLRLGNWLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGLDTIHTKKP
ILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL
Sequence of entity 12 (F, G, H), FASTA
>8W7M_12 DNA polymerase alpha-binding protein (chains F, G, H)
MVSVIDKLVFDFGGKTLVSLAPDNNTLCVANKNGLTKILKTNNPEEEPETLDSSKLVSSI
KCYSNSHFLMTTMQGDALRYNIDSSQEELLARFALPLRDCCVIHSGKMAVFGGDDLELIL
LELDDETHKKHAIKIDEQVSQISYNSQMNILAVSMINGKVQIFSLTSTIPNKVHELNDYI
VANSYDDTHRDKILSNMMDDIDKDNDNDLSETADPDENNVADPEFCAANRICTRVAWHPK
GLHFALPCADDTVKIFSIKGYSLQKTLSTNLSSTKAHFIDLQFDPLRGTYIAAVDLNNKL
TVWNWETSEIHYTREFKRKITNIAWKIQADSKTLDLVLGTWSGSIAIVQNLAESVVSNIP
DQSVAESSTKHGLFVDSESDLENLEGNDDINKSDKLFSDITQEANAEDVFTQTHDGPSGL
SEKRKYNFEDEEDFIDDDDGAGYISGKKPHNEHSYSRVHKTHSFPISLANTGKFRYMPFS
PAGTPFGFTDRRYLTMNEVGYVSTVKNSEQYSITVSFFDVGRFREYHFEDLFGYDLCFLN
EKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSF
NQFGVPFAVEKTSPIVALTAQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMS
LPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPEESKWLP
ILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPV
FVKSKLLEENKAILNKKNEIGADTEAEEGEEDKEIQIPVSMAAEEEYLRSKVLSELLTDT
LENDGEMYGNENEVLAALNGAYDKALLRLFASACSDQNVEKALSLAHELKQDRALTAAVK
ISERAELPSLVKKINNIREARYEQQLK
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| AGS | Phosphothiophosphoric acid-adenylate ester | C10 H16 N5 O12 P3 S | 2 |
| ZN | Zinc ion | Zn | 5 |
| MG | Magnesium ion | Mg | 4 |
| ADP | Adenosine-5'-diphosphate | C10 H15 N5 O10 P2 | 2 |
Primary citation
Synergism between CMG helicase and leading strand DNA polymerase at replication fork. Xu, Z., Feng, J., Yu, D. et al. Nat Commun (2023) 14:5849-5849. DOI 10.1038/s41467-023-41506-0 · PubMed
Other PDB entries of the same protein (UniProt P29469 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7W8G 2.52 Å, Cryo-EM structure of MCM double hexamer
- 28VY 2.7 Å, sCMGE assembled on ARS1 DNA with Sld2 and RPA
- 8RIF 2.79 Å, Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
- 7V3V 2.9 Å, Cryo-EM structure of MCM double hexamer bound with DDK in State I
- 9TH8 2.9 Å, Cryo-EM structure of MCM2-7 DH bound to Sld3-Sld7, Cdc45 and DNA
- 7P30 3.0 Å, 3.0 A resolution structure of a DNA-loaded MCM double hexamer
- 8KG6 3.07 Å, Yeast replisome in state I
- 9RHL 3.1 Å, Phospho-DH bound by Sld3-MBD on ARS1 DNA
- 7PMK 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
- 7PMN 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
- 7PT6 3.2 Å, Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III
- 7V3U 3.2 Å, Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD
Browse structure collections
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