8WE7: PDB entry 8WE7

Human L-type voltage-gated calcium channel Cav1.2 in the presence of calciseptine at 3.2 Angstrom resolution. Determined by electron microscopy at 3.2 Å resolution. Released 6 Dec 2023.

Method: Electron microscopy
Resolution: 3.2 Å
Organisms: Homo sapiens, Dendroaspis polylepis polylepis
Chains: 4
Atoms: 21,014
Mol. weight: 437.46 kDa
Ligands: CLR, CA, NAG
Released: 6 Dec 2023

Explore 8WE7 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8WE7 contains 119 α-helices and 86 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 66 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	124-143	20
α-helix	148-150	3
α-helix	154-181	28
α-helix	195-216	22
α-helix	233-236	4
α-helix	239-241	3
α-helix	243-248	6
α-helix	252-265	14
α-helix	270-276	7
α-helix	281-289	9
β-strand	297-298	2	1
β-strand	309	1	2
β-strand	311	1	2
β-strand	315	1	1
β-strand	332-333	2	1
α-helix	355-360	6
α-helix	365-376	12
α-helix	382-385	4
α-helix	397-416	20
α-helix	420-446	27
α-helix	515-521	7
α-helix	525-542	18
α-helix	550-578	29
α-helix	581-585	5
α-helix	587-601	15
α-helix	605-608	4
α-helix	619-624	6
α-helix	625-629	5
α-helix	634-646	13
α-helix	648-672	25
α-helix	692-703	12
α-helix	708-718	11
α-helix	726-728	3
α-helix	730-766	37
α-helix	768-774	7
α-helix	777-782	6
α-helix	901-918	18
α-helix	929-952	4
α-helix	959-972	14
α-helix	988-1003	16
α-helix	1014-1017	4
α-helix	1019-1023	5
α-helix	1024-1027	4
α-helix	1035-1068	34
β-strand	1076-1078	3	3
β-strand	1092-1096	5	4
α-helix	1097-1099	3
β-strand	1104-1108	5	4
β-strand	1111-1113	3	3
α-helix	1121-1132	12
α-helix	1137-1144	8
α-helix	1163-1169	7
α-helix	1170-1176	7
α-helix	1177-1191	15
α-helix	1194-1196	3
α-helix	1206-1214	9
α-helix	1228-1236	9
α-helix	1241-1256	16
α-helix	1265-1297	33
α-helix	1302-1321	20
α-helix	1373-1376	4
α-helix	1380-1389	10
α-helix	1392-1407	16
α-helix	1412-1429	18
β-strand	1433	1	5
α-helix	1450-1461	12
α-helix	1466-1472	7
β-strand	1477	1	6
β-strand	1479	1	5
β-strand	1495	1	6
α-helix	1500-1525	26
α-helix	1527-1530	4
α-helix	1539-1550	12
α-helix	1564-1571	8
α-helix	1586-1593	8
α-helix	1606-1617	12
α-helix	1626-1632	7
α-helix	1638-1639	2
α-helix	1646-1648	3

Chain B: 0 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	31
β-strand	16	1	31
β-strand	23-26	4	32
β-strand	34-37	4	32
β-strand	49-52	4	32

Chain C: 16 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	40-52	13
α-helix	59-60	2
β-strand	63	1	27
β-strand	65	1	28
α-helix	84-87	4
β-strand	94	1	27
β-strand	103-105	3	29
β-strand	117-119	3	29
α-helix	120	1
α-helix	121-127	7
α-helix	129-133	5
β-strand	172	1	28
α-helix	179	1
β-strand	180-183	4	30
α-helix	192-199	8
α-helix	203-207	5
α-helix	241-244	4
α-helix	253-261	9
α-helix	279-282	4
β-strand	288-292	5	30
α-helix	297-304	8
α-helix	311-313	3
α-helix	316-323	8
β-strand	335-337	3	30
α-helix	342-358	17

Chain D: 37 helices, 59 β-strands

Element	Residues	Length	Sheet
α-helix	29-52	24
α-helix	54-63	10
α-helix	65-67	3
β-strand	68-72	5	7
α-helix	75-108	34
β-strand	122-123	2	8
β-strand	125-126	2	9
β-strand	142-143	2	8
β-strand	150-151	2	10
β-strand	158-159	2	10
β-strand	160	1	11
β-strand	165-167	3	9
α-helix	179-185	7
α-helix	190-199	10
β-strand	206-210	5	9
β-strand	215-218	4	9
β-strand	220	1	11
α-helix	238-240	3
α-helix	242-248	7
β-strand	252	1	12
β-strand	255-259	5	13
α-helix	262-264	3
α-helix	267-279	13
α-helix	280-282	3
β-strand	288-289	2	14
β-strand	290-294	5	13
β-strand	298-300	3	13
β-strand	308-309	2	14
α-helix	314-317	4
α-helix	333-343	11
β-strand	355	1	12
β-strand	357-362	6	13
α-helix	372-375	4
β-strand	383-389	7	13
α-helix	397-405	9
β-strand	409-413	5	13
α-helix	419-422	4
α-helix	426-429	4
α-helix	432-435	4
α-helix	447-449	3
β-strand	450-451	2	15
β-strand	458-461	4	15
β-strand	465-467	3	9
β-strand	484-490	7	9
β-strand	491-493	3	15
α-helix	494-499	6
β-strand	512-515	4	16
β-strand	520	1	17
β-strand	521-523	3	16
α-helix	531-533	3
β-strand	560	1	17
α-helix	570-580	11
β-strand	584-585	2	16
β-strand	588-593	6	18
β-strand	600-605	6	18
β-strand	608-611	4	16
β-strand	618-621	4	16
β-strand	629-633	5	7
α-helix	640-643	4
β-strand	658-659	2	19
α-helix	676-687	12
α-helix	700-716	17
α-helix	719-722	4
β-strand	732-735	4	20
β-strand	741	1	20
β-strand	742-743	2	19
α-helix	747-750	4
α-helix	758-760	3
α-helix	762-769	8
β-strand	774-776	3	20
α-helix	777-779	3
β-strand	792-795	4	20
β-strand	800	1	21
β-strand	807	1	21
β-strand	811-816	6	20
α-helix	819-826	8
β-strand	848-854	7	22
β-strand	858	1	23
β-strand	859-861	3	22
β-strand	874	1	23
α-helix	880-888	9
β-strand	892-894	3	24
β-strand	897-904	8	25
β-strand	972-978	7	25
β-strand	981-983	3	24
β-strand	989	1	22
β-strand	993-995	3	26
β-strand	1000-1002	3	26
β-strand	1003-1007	5	22
β-strand	1013-1019	7	22
α-helix	1021-1023	3
β-strand	1036-1038	3	25
α-helix	1040	1
α-helix	1042	1
α-helix	1043-1046	4
α-helix	1050-1052	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Voltage-dependent L-type calcium channel subunit alpha-1C	A	protein	2201	Homo sapiens	Q13936 (AlphaFold model)
Voltage-dependent calcium channel subunit alpha-2/delta-1	D	protein	1103	Homo sapiens	P54289 (AlphaFold model)
Voltage-dependent L-type calcium channel subunit beta-3	C	protein	484	Homo sapiens	P54284 (AlphaFold model)
Calciseptin	B	protein	60	Dendroaspis polylepis polylepis	P22947 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>8WE7_1 Voltage-dependent L-type calcium channel subunit alpha-1C (chains A)
MVNENTRMYIPEENHQGSNYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAAR
QAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISI
VEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIA
YGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFR
VLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYN
QEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCI
TMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGD
FQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSMPTSETESVNTENVA
GGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLT
IASEHYNQPNWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFVVCGGI
LETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFL
FIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYG
GPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLAR
TASPEKKQELVEKPAVGESKEEKIELKSITADGESPPATKINMDDLQPNENEDKSPYPNP
ETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSSNNRFRLQCHRIVNDT
IFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLH
KGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQ
CVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGE
VDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRV
EISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLR
RYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTG
LFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNHYFCDAWNTFDALIVV
GSIVDIAITEVNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWT
FIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRC
ATGEAWQDIMLACMPGKKCAPESEPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFV
AVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGK
LCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKI
WKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQ
AGLRTLHDIGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVSYYQS
DGRSAFPQTFTTQRPLHINKAGSSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANN
TALGRLPRPAGYPSTVSTVEGHGPPLSPAIRVQEVAWKLSSNRERHVPMCEDLELRRDSG
SAGTQAHCLLLRKANPSRCHSRESQAAMAGQEETSQDETYEVKMNHDTEACSEPSLLSTE
MLSYQDDENRQLTLPEEDKRDIRQSPKRGFLRSASLGRRASFHLECLKRQKDRGGDISQK
TVLPLHLVHHQALAVAGLSPLLQRSHSPASFPRPFATPPATPGSRGWPPQPVPTLRLEGV
ESSEKLNSSFPSIHCGSWAETTPGGGGSSAARRVRPVSLMVPSQAGAPGRQFHGSASSLV
EAVLISEGLGQFAQDPKFIEVTTQELADACDMTIEEMESAADNILSGGAPQSPNGALLPF
VNCRDAGQDRAGGEEDAGCVRARGRPSEEELQDSRVYVSSL

Sequence of entity 2 (D), FASTA

>8WE7_2 Voltage-dependent calcium channel subunit alpha-2/delta-1 (chains D)
MAAGCLLALTLTLFQSLLIGPSSEEPFPSAVTIKSWVDKMQEDLVTLAKTASGVNQLVDI
YEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLALEAEKVQAAHQWREDFASN
EVVYYNAKDDLDPEKNDSEPGSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVL
NELNWTSALDEVFKKNREEDPSLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRR
RPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQD
VSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIML
FTDGGEERAQEIFNKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIR
INTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNITGQFENKTNLK
NQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKPIGVGIPTIN
LRKRRPNIQNPKSQEPVTLDFLDAELENDIKVEIRNKMIDGESGEKTFRTLVKSQDERYI
DKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKLEETITQARYSETLKPDNFEESGYTF
IAPRDYCNDLKISDNNTEFLLNFNEFIDRKTPNNPSCNADLINRVLLDAGFTNELVQNYW
SKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYF
NKSGPGAYESGIMVSKAVEIYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCD
CKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSLMRHLVNISVYAFNKSYDY
QSVCEPGAAPKQGAGHRSAYVPSVADILQIGWWATAAAWSILQQFLLSLTFPRLLEAVEM
EDDDFTASLSKQSCITEQTQYFFDNDSKSFSGVLDCGNCSRIFHGEKLMNTNLIFIMVES
KGTCPCDTRLLIQAEQTSDGPNPCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPS
LWYIIGIQFLLLWLVSGSTHRLL

Sequence of entity 3 (C), FASTA

>8WE7_3 Voltage-dependent L-type calcium channel subunit beta-3 (chains C)
MYDDSYVPGFEDSEAGSADSYTSRPSLDSDVSLEEDRESARREVESQAQQQLERAKHKPV
AFAVRTNVSYCGVLDEECPVQGSGVNFEAKDFLHIKEKYSNDWWIGRLVKEGGDIAFIPS
PQRLESIRLKQEQKARRSGNPSSLSDIGNRRSPPPSLAKQKQKQAEHVPPYDVVPSMRPV
VLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADLSLAKRSVLNNPGKRTIIER
SSARSSIAEVQSEIERIFELAKSLQLVVLDADTINHPAQLAKTSLAPIIVFVKVSSPKVL
QRLIRSRGKSQMKHLTVQMMAYDKLVQCPPESFDVILDENQLEDACEHLAEYLEVYWRAT
HHPAPGPGLLGPPSAIPGLQNQQLLGERGEEHSPLERDSLMPSDEASESSRQAWTGSSQR
SSRHLEEDYADAYQDLYQPHRQHTSGLPSANGHDPQDRLLAQDSEHNHSDRNWQRNRPWP
KDSY

Sequence of entity 4 (B), FASTA

>8WE7_4 Calciseptin (chains B)
RICYIHKASLPRATKTCVENTCYKMFIRTQREYISERGCGCPTAMWPYQTECCKGDRCNK

Ligands and cofactors

ID	Name	Formula	Copies
CLR	Cholesterol	C27 H46 O	2
CA	Calcium ion	Ca	2
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	3

Primary citation

Structural basis for human Ca v 1.2 inhibition by multiple drugs and the neurotoxin calciseptine. Gao, S., Yao, X., Chen, J. et al. Cell (2023) 186:5363-5374.e16. DOI 10.1016/j.cell.2023.10.007 · PubMed

Other PDB entries of the same protein (UniProt Q13936 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2F3Y 1.45 Å, Calmodulin/IQ domain complex
2F3Z 1.6 Å, Calmodulin/IQ-AA domain complex
6DAD 1.65 Å, 1.65 Angstrom crystal structure of the N97I Ca/CaM:CaV1.2 IQ domain complex
6U3A 1.65 Å, 1.65 Angstrom crystal structure of the N97S Ca-CaM:CaV1.2 IQ domain complex
5V2Q 1.7 Å, CaV beta2a subunit: CaV1.2 AID-CEN complex
6U3B 1.7 Å, 1.7 Angstrom crystal structure of the Q135P Ca-CaM:CaV1.2 IQ domain complex
6U3D 1.75 Å, 1.75 Angstrom crystal structure of the N53I Ca-CaM:CaV1.2 IQ domain complex
1T0J 2.0 Å, Crystal structure of a complex between voltage-gated calcium channel beta2a subunit and…
2BE6 2.0 Å, 2.0 A crystal structure of the CaV1.2 IQ domain-Ca/CaM complex
5V2P 2.0 Å, CaV beta2a subunit: CaV1.2 AID-CAP complex
6DAE 2.0 Å, 2.0 Angstrom crystal structure of the D95V Ca/CaM:CaV1.2 IQ domain complex
3G43 2.1 Å, Crystal structure of the calmodulin-bound Cav1.2 C-terminal regulatory domain dimer

Browse structure collections

About this viewer

MolViewer shows 8WE7 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.