8WE8: PDB entry 8WE8

Human L-type voltage-gated calcium channel Cav1.2 in the presence of calciseptine, amlodipine and pinaverium at 2.9 Angstrom resolution. Determined by electron microscopy at 2.9 Å resolution. Released 6 Dec 2023.

Method
Electron microscopy
Resolution
2.9 Å
Organisms
Homo sapiens, Dendroaspis polylepis polylepis
Chains
4
Atoms
21,309
Mol. weight
440.82 kDa
Ligands
CLR, CA, 6UB, 3PE
Released
6 Dec 2023

Explore 8WE8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8WE8 contains 117 α-helices and 80 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 66 helices, 10 β-strands

ElementResiduesLengthSheet
α-helix126-13510
α-helix148-1503
α-helix154-16916
α-helix175-1784
α-helix194-21522
α-helix234-24714
α-helix252-26211
α-helix266-2683
α-helix269-28921
β-strand296-29831
β-strand332-33431
α-helix349-36012
α-helix365-37511
α-helix382-3898
α-helix390-3956
α-helix396-41520
α-helix420-44627
α-helix511-52111
α-helix525-54218
α-helix550-57728
α-helix580-5856
α-helix587-60317
α-helix612-6143
α-helix616-62611
α-helix627-6304
α-helix634-64613
α-helix648-67326
α-helix684-6852
α-helix692-70413
α-helix708-71710
α-helix726-7294
α-helix730-78152
α-helix901-91818
α-helix928-97225
α-helix989-100315
α-helix1014-10174
α-helix1019-10224
α-helix1025-10284
α-helix1035-107036
β-strand1076-107832
β-strand1092-109653
α-helix1097-10993
β-strand1104-110853
β-strand1111-111332
α-helix1121-113212
α-helix1137-114610
α-helix1160-11623
α-helix1163-11697
α-helix1170-11778
α-helix1178-119013
α-helix1194-11963
α-helix1206-12149
α-helix1229-12368
α-helix1239-125618
α-helix1265-129127
α-helix1294-12985
α-helix1302-132120
α-helix1376-13783
α-helix1379-13824
α-helix1385-13884
α-helix1392-140716
α-helix1410-143021
β-strand143314
α-helix1450-146213
α-helix1466-14727
β-strand147715
β-strand147914
β-strand149515
α-helix1500-152526
α-helix1527-15304
α-helix1539-155012
α-helix1564-15718
α-helix1586-15938
α-helix1606-161712
α-helix1626-16338
α-helix1638-16392
Chain B: 1 helix, 3 β-strands
ElementResiduesLengthSheet
β-strand23-27527
β-strand34-37427
α-helix42-443
β-strand48-52527
Chain C: 8 helices, 11 β-strands
ElementResiduesLengthSheet
α-helix40-5112
α-helix58-603
β-strand63-66424
β-strand91-93324
β-strand94-97425
β-strand103-107525
β-strand116-119425
α-helix121-13212
β-strand171-174424
β-strand180-183426
α-helix193-20715
β-strand213-214226
α-helix252-26211
β-strand267-268226
β-strand288-291426
α-helix298-3069
α-helix315-32713
β-strand335-336226
α-helix342-35918
Chain D: 42 helices, 56 β-strands
ElementResiduesLengthSheet
α-helix30-5223
α-helix54-6310
α-helix65-673
β-strand68-7256
α-helix75-11036
β-strand122-12437
β-strand125-12628
β-strand142-14437
β-strand150-15129
β-strand158-15929
β-strand160110
β-strand165-16738
α-helix177-1859
α-helix190-19910
β-strand206-21058
β-strand215-21848
β-strand220110
α-helix238-2403
α-helix242-2487
β-strand252111
β-strand255-259512
α-helix262-2643
α-helix267-28014
β-strand289113
β-strand291-294412
β-strand298-300312
β-strand308113
α-helix312-32211
α-helix333-34311
β-strand355111
β-strand357-362612
α-helix370-3767
β-strand383-389712
α-helix397-4059
β-strand410-413412
α-helix416-4183
α-helix419-4224
α-helix426-4305
α-helix432-4354
α-helix439-4413
β-strand450-451214
β-strand458-461414
β-strand464-46748
β-strand484-49078
β-strand491-493314
α-helix494-4996
β-strand511-515515
β-strand520116
β-strand521-523315
α-helix531-5333
β-strand560116
α-helix569-58012
β-strand584-5931015
β-strand600-6111215
β-strand618-622515
β-strand624115
β-strand629-63356
α-helix639-6457
α-helix646-6483
α-helix650-6523
α-helix653-6564
β-strand658-659217
α-helix676-68712
α-helix699-71517
α-helix716-7205
β-strand729-736818
β-strand741118
β-strand742-743217
α-helix747-7515
α-helix758-7603
α-helix762-7698
β-strand774-776318
α-helix777-7782
β-strand792-797618
β-strand800-801219
β-strand806-807219
β-strand810-816718
α-helix819-8268
β-strand848-849220
β-strand852-854321
β-strand858122
β-strand859121
α-helix868-8703
β-strand874122
α-helix875-8773
α-helix880-8889
β-strand892-9041323
β-strand972-9831223
β-strand989-994621
β-strand1001-1007721
α-helix10081
β-strand1013-1017521
β-strand1018-1019220
α-helix1021-10233
α-helix1028-10314
β-strand1036-1038323
α-helix1043-10464
α-helix1050-10523

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Voltage-dependent L-type calcium channel subunit alpha-1CAprotein2201Homo sapiensQ13936 (AlphaFold model)
Voltage-dependent calcium channel subunit alpha-2/delta-1Dprotein1103Homo sapiensP54289 (AlphaFold model)
Voltage-dependent L-type calcium channel subunit beta-3Cprotein484Homo sapiensP54284 (AlphaFold model)
CalciseptinBprotein60Dendroaspis polylepis polylepisP22947 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>8WE8_1 Voltage-dependent L-type calcium channel subunit alpha-1C (chains A)
MVNENTRMYIPEENHQGSNYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAAR
QAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISI
VEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIA
YGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFR
VLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYN
QEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCI
TMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGD
FQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGMDEEKPRNMSMPTSETESVNTENVA
GGDIEGENCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSNVFYWLVIFLVFLNTLT
IASEHYNQPNWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFVVCGGI
LETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFL
FIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYG
GPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLAR
TASPEKKQELVEKPAVGESKEEKIELKSITADGESPPATKINMDDLQPNENEDKSPYPNP
ETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSSNNRFRLQCHRIVNDT
IFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLH
KGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQ
CVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGE
VDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRV
EISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLR
RYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTG
LFTVEMILKLIAFKPKGYFSDPWNVFDFLIVIGSIIDVILSETNHYFCDAWNTFDALIVV
GSIVDIAITEVNPAEHTQCSPSMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWT
FIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRC
ATGEAWQDIMLACMPGKKCAPESEPSNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFV
AVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGK
LCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKI
WKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQ
AGLRTLHDIGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVSYYQS
DGRSAFPQTFTTQRPLHINKAGSSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANN
TALGRLPRPAGYPSTVSTVEGHGPPLSPAIRVQEVAWKLSSNRERHVPMCEDLELRRDSG
SAGTQAHCLLLRKANPSRCHSRESQAAMAGQEETSQDETYEVKMNHDTEACSEPSLLSTE
MLSYQDDENRQLTLPEEDKRDIRQSPKRGFLRSASLGRRASFHLECLKRQKDRGGDISQK
TVLPLHLVHHQALAVAGLSPLLQRSHSPASFPRPFATPPATPGSRGWPPQPVPTLRLEGV
ESSEKLNSSFPSIHCGSWAETTPGGGGSSAARRVRPVSLMVPSQAGAPGRQFHGSASSLV
EAVLISEGLGQFAQDPKFIEVTTQELADACDMTIEEMESAADNILSGGAPQSPNGALLPF
VNCRDAGQDRAGGEEDAGCVRARGRPSEEELQDSRVYVSSL
Sequence of entity 2 (D), FASTA
>8WE8_2 Voltage-dependent calcium channel subunit alpha-2/delta-1 (chains D)
MAAGCLLALTLTLFQSLLIGPSSEEPFPSAVTIKSWVDKMQEDLVTLAKTASGVNQLVDI
YEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLALEAEKVQAAHQWREDFASN
EVVYYNAKDDLDPEKNDSEPGSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVL
NELNWTSALDEVFKKNREEDPSLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRR
RPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQD
VSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIML
FTDGGEERAQEIFNKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIR
INTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNITGQFENKTNLK
NQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKPIGVGIPTIN
LRKRRPNIQNPKSQEPVTLDFLDAELENDIKVEIRNKMIDGESGEKTFRTLVKSQDERYI
DKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKLEETITQARYSETLKPDNFEESGYTF
IAPRDYCNDLKISDNNTEFLLNFNEFIDRKTPNNPSCNADLINRVLLDAGFTNELVQNYW
SKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYF
NKSGPGAYESGIMVSKAVEIYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCD
CKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSLMRHLVNISVYAFNKSYDY
QSVCEPGAAPKQGAGHRSAYVPSVADILQIGWWATAAAWSILQQFLLSLTFPRLLEAVEM
EDDDFTASLSKQSCITEQTQYFFDNDSKSFSGVLDCGNCSRIFHGEKLMNTNLIFIMVES
KGTCPCDTRLLIQAEQTSDGPNPCDMVKQPRYRKGPDVCFDNNVLEDYTDCGGVSGLNPS
LWYIIGIQFLLLWLVSGSTHRLL
Sequence of entity 3 (C), FASTA
>8WE8_3 Voltage-dependent L-type calcium channel subunit beta-3 (chains C)
MYDDSYVPGFEDSEAGSADSYTSRPSLDSDVSLEEDRESARREVESQAQQQLERAKHKPV
AFAVRTNVSYCGVLDEECPVQGSGVNFEAKDFLHIKEKYSNDWWIGRLVKEGGDIAFIPS
PQRLESIRLKQEQKARRSGNPSSLSDIGNRRSPPPSLAKQKQKQAEHVPPYDVVPSMRPV
VLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADLSLAKRSVLNNPGKRTIIER
SSARSSIAEVQSEIERIFELAKSLQLVVLDADTINHPAQLAKTSLAPIIVFVKVSSPKVL
QRLIRSRGKSQMKHLTVQMMAYDKLVQCPPESFDVILDENQLEDACEHLAEYLEVYWRAT
HHPAPGPGLLGPPSAIPGLQNQQLLGERGEEHSPLERDSLMPSDEASESSRQAWTGSSQR
SSRHLEEDYADAYQDLYQPHRQHTSGLPSANGHDPQDRLLAQDSEHNHSDRNWQRNRPWP
KDSY
Sequence of entity 4 (B), FASTA
>8WE8_4 Calciseptin (chains B)
RICYIHKASLPRATKTCVENTCYKMFIRTQREYISERGCGCPTAMWPYQTECCKGDRCNK

Ligands and cofactors

IDNameFormulaCopies
CLRCholesterolC27 H46 O5
CACalcium ionCa2
6UBamlodipineC20 H25 Cl N2 O51
3PE1,2-Distearoyl-sn-glycerophosphoethanolamineC41 H82 N O8 P1
PT5[(2R)-1-octadecanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4…C47 H85 O19 P31
NAG2-acetamido-2-deoxy-beta-D-glucopyranoseC8 H15 N O63

Primary citation

Structural basis for human Ca v 1.2 inhibition by multiple drugs and the neurotoxin calciseptine. Gao, S., Yao, X., Chen, J. et al. Cell (2023) 186:5363-5374.e16. DOI 10.1016/j.cell.2023.10.007 · PubMed

Other PDB entries of the same protein (UniProt Q13936 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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