8WG9: MGlu2-mGlu4 heterodimer bound mGlu4 agonist E7P

mGlu2-mGlu4 heterodimer bound mGlu4 agonist E7P. Determined by electron microscopy at 4.46 Å resolution. Released 27 Nov 2024.

Method: Electron microscopy
Resolution: 4.46 Å
Organism: Homo sapiens
Chains: 2
Atoms: 11,806
Mol. weight: 192.41 kDa
Ligands: E7P
Released: 27 Nov 2024

Explore 8WG9 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8WG9 contains 80 α-helices and 57 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain B: 37 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	43-45	3	1
β-strand	49-55	7	1
β-strand	58	1	2
β-strand	70	1	2
α-helix	76-90	15
β-strand	101-107	7	1
α-helix	113-126	14
β-strand	150-154	5	1
α-helix	159-169	11
α-helix	170-172	3
β-strand	176-178	3	1
α-helix	184-187	4
β-strand	195-197	3	1
α-helix	202-215	14
β-strand	220-226	7	3
α-helix	229-245	17
β-strand	249-256	8	3
α-helix	260-261	2
α-helix	264-272	9
β-strand	280-283	4	3
α-helix	287-299	13
β-strand	307-310	4	3
β-strand	332-336	5	3
α-helix	342-350	9
α-helix	362-369	8
α-helix	394-396	3
α-helix	406-427	22
α-helix	436-438	3
α-helix	443-452	10
β-strand	455-456	2	4
α-helix	458-460	3
β-strand	462-463	2	4
β-strand	475-481	7	3
β-strand	488-495	8	3
β-strand	499-501	3	3
α-helix	522-523	2
β-strand	528-532	5	5
β-strand	540-544	5	5
α-helix	545	1
β-strand	549-553	5	6
β-strand	556-559	4	6
β-strand	564-566	3	7
β-strand	573-575	3	7
α-helix	576-577	2
β-strand	578-580	3	8
α-helix	589-611	23
α-helix	616-619	4
α-helix	623-642	20
α-helix	646-647	2
α-helix	649-683	35
α-helix	690-691	2
α-helix	696-720	25
β-strand	725-732	8	8
α-helix	737-739	3
β-strand	741-746	6	8
α-helix	750-773	24
α-helix	774-776	3
α-helix	784-799	16
α-helix	801-807	7
α-helix	808-810	3
α-helix	814-833	20
α-helix	834-838	5
α-helix	839-847	9
α-helix	849-851	3

Chain C: 43 helices, 30 β-strands

Element	Residues	Length	Sheet
β-strand	28	1	9
β-strand	32-38	7	9
β-strand	41-43	3	10
β-strand	50-53	4	10
α-helix	55-59	5
α-helix	60-73	14
β-strand	84-90	7	9
α-helix	95-102	8
α-helix	104-107	4
β-strand	140-141	2	9
α-helix	145-155	11
α-helix	156-158	3
β-strand	162-164	3	9
β-strand	181-183	3	9
α-helix	188-190	3
α-helix	191-201	11
β-strand	207-212	6	11
α-helix	215-230	16
α-helix	233-234	2
β-strand	235-241	7	11
α-helix	247-258	12
β-strand	261	1	12
β-strand	263	1	12
β-strand	265-268	4	11
α-helix	272-284	13
β-strand	290-293	4	11
α-helix	295-297	3
α-helix	310-313	4
β-strand	314-319	6	11
α-helix	320-321	2
α-helix	325-332	8
α-helix	345-352	8
α-helix	362-364	3
α-helix	367-369	3
α-helix	378-399	22
α-helix	408-410	3
α-helix	416-418	3
α-helix	419-423	5
β-strand	428	1	13
α-helix	429-430	2
β-strand	441	1	13
β-strand	443	1	14
β-strand	448	1	9
β-strand	449	1	14
β-strand	453-459	7	11
β-strand	467-474	8	11
β-strand	478-480	3	11
α-helix	482-484	3
α-helix	490-492	3
α-helix	494-495	2
α-helix	497	1
β-strand	505	1	15
β-strand	508-510	3	15
α-helix	517-519	3
β-strand	522-524	3	15
β-strand	529-532	4	16
β-strand	536-539	4	16
α-helix	569-591	23
α-helix	596-599	4
α-helix	603-622	20
α-helix	626-627	2
α-helix	629-646	18
α-helix	650-657	8
α-helix	676-698	23
β-strand	706	1	17
β-strand	719	1	17
α-helix	720-721	2
α-helix	727-729	3
α-helix	730-740	11
α-helix	746-751	6
α-helix	756-776	21
α-helix	779-782	4
α-helix	787-819	33

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Metabotropic glutamate receptor 4	B	protein	880	Homo sapiens	Q14833 (AlphaFold model)
Metabotropic glutamate receptor 2	C	protein	854	Homo sapiens	Q14416 (AlphaFold model)

Sequence of entity 1 (B), FASTA

>8WG9_1 Metabotropic glutamate receptor 4 (chains B)
KPKGHPHMNSIRIDGDITLGGLFPVHGRGSEGKPCGELKKEKGIHRLEAMLFALDRINND
PDLLPNITLGARILDTCSRDTHALEQSLTFVQALIEKDGTEVRCGSGGPPIITKPERVVG
VIGASGSSVSIMVANILRLFKIPQISYASTAPDLSDNSRYDFFSRVVPSDTYQAQAMVDI
VRALKWNYVSTVASEGSYGESGVEAFIQKSREDGGVCIAQSVKIPREPKAGEFDKIIRRL
LETSNARAVIIFANEDDIRRVLEAARRANQTGHFFWMGSDSWGSKIAPVLHLEEVAEGAV
TILPKRMSVRGFDRYFSSRTLDNNRRNIWFAEFWEDNFHCKLSRHALKKGSHVKKCTNRE
RIGQDSAYEQEGKVQFVIDAVYAMGHALHAMHRDLCPGRVGLCPRMDPVDGTQLLKYIRN
VNFSGIAGNPVTFNENGDAPGRYDIYQYQLRNDSAEYKVIGSWTDHLHLRIERMHWPGSG
QQLPRSICSLPCQPGERKKTVKGMPCCWHCEPCTGYQYQVDRYTCKTCPYDMRPTENRTG
CRPIPIIKLEWGSPWAVLPLFLAVVGIAATLFVVITFVRYNDTPIVKASGRELSYVLLAG
IFLCYATTFLMIAEPDLGTCSLRRIFLGLGMSISYAALLTKTNRIYRIFEQGKRSVSAPR
FISPASQLAITFSLISLQLLGICVWFVVDPSHSVVDFQDQRTLDPRFARGVLKCDISDLS
LICLLGYSMLLMVTCTVYAIKTRGVPETFNEAKPIGFTMYTTCIVWLAFIPIFFGTSQSA
DKLYIQTTTLTVSVSLSASVSLGMLYMPKVYIILFHPEQNVPKRKRSLKAVVTAATMSNK
FTQKGNFRPNGEAKSELCENLEAPALATKQTYVTYTNHAI

Sequence of entity 2 (C), FASTA

>8WG9_2 Metabotropic glutamate receptor 2 (chains C)
EGPAKKVLTLEGDLVLGGLFPVHQKGGPAEDCGPVNEHRGIQRLEAMLFALDRINRDPHL
LPGVRLGAHILDSCSKDTHALEQALDFVRASLSRGADGSRHICPDGSYATHGDAPTAITG
VIGGSYSDVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFFQAKAMAEI
LRFFNWTYVSTVASEGDYGETGIEAFELEARARNICVATSEKVGRAMSRAAFEGVVRALL
QKPSARVAVLFTRSEDARELLAASQRLNASFTWVASDGWGALESVVAGSEGAAEGAITIE
LASYPISDFASYFQSLDPWNNSRNPWFREFWEQRFRCSFRQRDCAAHSLRAVPFEQESKI
MFVVNAVYAMAHALHNMHRALCPNTTRLCDAMRPVNGRRLYKDFVLNVKFDAPFRPADTH
NEVRFDRFGDGIGRYNIFTYLRAGSGRYRYQKVGYWAEGLTLDTSLIPWASPSAGPLPAS
RCSEPCLQNEVKSVQPGEVCCWLCIPCQPYEYRLDEFTCADCGLGYWPNASLTGCFELPQ
EYIRWGDAWAVGPVTIACLGALATLFVLGVFVRHNATPVVKASGRELCYILLGGVFLCYC
MTFIFIAKPSTAVCTLRRLGLGTAFSVCYSALLTKTNRIARIFGGAREGAQRPRFISPAS
QVAICLALISGQLLIVVAWLVVEAPGTGKETAPERREVVTLRCNHRDASMLGSLAYNVLL
IALCTLYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFLPIFYVTSSDYRVQTTTMCVSV
SLSGSVVLGCLFAPKLHIILFQPQKNVVSHRAPTSRFGSAAARASSSLGQGSGSQFVPTV
CNGREVVDSTTSSL

Ligands and cofactors

ID	Name	Formula	Copies
E7P	(2S)-2-amino-4-phosphonobutanoic acid	C4 H10 N O5 P	1

Primary citation

Structural basis of orientated asymmetry in a mGlu heterodimer. Huang, W., Jin, N., Guo, J. et al. Nat Commun (2024) 15:10345-10345. DOI 10.1038/s41467-024-54744-7 · PubMed

Other PDB entries of the same protein (UniProt Q14833 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8JD4 2.9 Å, Cryo-EM structure of G protein-free mGlu2-mGlu4 heterodimer in Acc state
8JD6 3.4 Å, Cryo-EM structure of Gi1-bound metabotropic glutamate receptor mGlu4
8JD5 3.6 Å, Cryo-EM structure of Gi1-bound mGlu2-mGlu4 heterodimer
8WGB 3.7 Å, mGlu2-4 heterodimer bound with Gi
7E9H 4.0 Å, Cryo-EM structure of Gi-bound metabotropic glutamate receptor mGlu4
8WGD 4.45 Å, mGlu2-4 inactive heterodimer
8WGC 5.95 Å, heterodimer of mGlu2 and mGlu4 bound with mGlu2 agonist LY379268

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