8XGS: A peptide receptor complex structure

a peptide receptor complex structure. Determined by electron microscopy at 2.95 Å resolution. Released 30 Oct 2024.

Method: Electron microscopy
Resolution: 2.95 Å
Organisms: Homo sapiens, synthetic construct
Chains: 6
Atoms: 8,996
Mol. weight: 164.72 kDa
Released: 30 Oct 2024

Explore 8XGS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8XGS contains 35 α-helices and 66 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 14 helices, 0 β-strands

Element	Residues	Length
α-helix	41-69	29
α-helix	76-82	7
α-helix	86-101	16
α-helix	112-145	34
α-helix	156-174	19
α-helix	175-179	5
α-helix	198-209	12
α-helix	210-214	5
α-helix	215-222	8
α-helix	226-235	10
α-helix	246-286	41
α-helix	299-320	22
α-helix	321-325	5
α-helix	328-335	8

Chain B: 4 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	9-24	16
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	1
β-strand	61-63	3	2
β-strand	69-74	6	2
β-strand	78-83	6	2
β-strand	88-94	7	2
β-strand	103-105	3	3
β-strand	111-114	4	3
β-strand	116	1	4
β-strand	121-125	5	3
β-strand	134-139	6	3
α-helix	145	1
β-strand	146-153	8	4
β-strand	156-161	6	4
β-strand	166-168	3	4
β-strand	177-180	4	4
β-strand	187-192	6	5
β-strand	199-203	5	5
β-strand	207-210	4	5
β-strand	220-223	4	5
β-strand	229-234	6	6
β-strand	240-245	6	6
β-strand	250-254	5	6
β-strand	259-264	6	6
β-strand	273-278	6	7
β-strand	285-289	5	7
β-strand	296-297	2	7
β-strand	304-306	3	7
β-strand	315-320	6	1
β-strand	327-331	5	1
β-strand	336-339	4	1

Chain C: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	10-22	13
α-helix	30-43	14

Chain D: 5 helices, 30 β-strands

Element	Residues	Length	Sheet
β-strand	20	1	8
β-strand	23-24	2	9
β-strand	27-29	3	10
β-strand	34-40	7	9
β-strand	42	1	8
α-helix	46-48	3
β-strand	50-52	3	11
β-strand	55-56	2	10
β-strand	62-63	2	10
β-strand	66	1	12
β-strand	67-68	2	11
β-strand	77	1	12
α-helix	79-81	3
β-strand	85-90	6	9
β-strand	95-101	7	9
α-helix	105-107	3
β-strand	109-110	2	10
β-strand	115-116	2	11
β-strand	133-136	4	10
β-strand	157-158	2	13
β-strand	163-164	2	14
β-strand	172-176	5	15
β-strand	177-178	2	13
β-strand	183	1	16
β-strand	189	1	16
β-strand	191-196	6	14
β-strand	203-207	5	14
β-strand	211-212	2	14
α-helix	213	1
β-strand	220-225	6	15
β-strand	228-233	6	15
β-strand	242-248	7	14
α-helix	254	1
β-strand	256	1	14
β-strand	260-262	3	14

Chain E: 10 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	9-29	21
β-strand	33-38	6	17
α-helix	46-56	11
β-strand	185-189	5	17
β-strand	194-198	5	17
α-helix	211-214	4
β-strand	219-225	7	17
α-helix	226-228	3
α-helix	241-246	6
α-helix	250-253	4
α-helix	256-260	5
β-strand	263-268	6	17
α-helix	270-274	5
α-helix	298-308	11
β-strand	318-319	2	17
β-strand	322	1	17
α-helix	330-349	20

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
KiSS-1 receptor	A	protein	398	Homo sapiens	Q969F8 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	357	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
scfv16	D	protein	261	Homo sapiens
Guanine nucleotide-binding protein G(q) subunit alpha	E	protein	353	Homo sapiens	P50148 (AlphaFold model)
Metastin	F	protein	55	synthetic construct	Q15726

Sequence of entity 1 (A), FASTA

>8XGS_1 KiSS-1 receptor (chains A)
MHTVATSGPNASWGAPANASGCPGCGANASDGPVPSPRAVDAWLVPLFFAALMLLGLVGN
SLVIYVICRHKPMRTVTNFYIANLAATDVTFLLCCVPFTALLYPLPGWVLGDFMCKFVNY
IQQVSVQATCATLTAMSVDRWYVTVFPLRALHRRTPRLALAVSLSIWVGSAAVSAPVLAL
HRLSPGPRAYCSEAFPSRALERAFALYNLLALYLLPLLATCACYAAMLRHLGRVAVRPAP
ADSALQGQVLAERAGAVRAKVSRLVAAVVLLFAACWGPIQLFLVLQALGPAGSWHPRSYA
AYALKTWAHCMSYSNSALNPLLYAFLGSHFRQAFRRVCPCAPRRPRRPRRPGPSDPAAPH
AELLRLGSHPAPARAQKPGSSGLAARGLCVLGEDNAPL

Sequence of entity 2 (B), FASTA

>8XGS_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
HHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRI
QMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTC
AYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGD
TTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFT
GHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGR
LLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (C), FASTA

>8XGS_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FFEKKFFCAIL

Sequence of entity 4 (D), FASTA

>8XGS_4 scfv16 (chains D)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKGSLEVLFQGPAAA

Sequence of entity 5 (E), FASTA

>8XGS_5 Guanine nucleotide-binding protein G(q) subunit alpha (chains E)
MGCTLSAEDKAAVERSKMIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSG
YSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFEN
PYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPAYLPTQQDVLRVRVPT
TGIIEYPFDLQSVIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNEN
RMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQAAR
EFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV

Sequence of entity 6 (F), FASTA

>8XGS_6 Metastin (chains F)
GTSLSPPPESSGSRQQPGLSAPHSRQIPAPQGAVLVQREKDLPNYNWNSFGLRFX

Primary citation

Structural basis for the ligand recognition and G protein subtype selectivity of kisspeptin receptor. Wu, Z., Chen, G., Qiu, C. et al. Sci Adv (2024) 10:eadn7771-eadn7771. DOI 10.1126/sciadv.adn7771 · PubMed

Other PDB entries of the same protein (UniProt Q969F8 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8XGO 2.68 Å, a peptide receptor complex structure
8XGU 3.0 Å, a peptide receptor complex structure
8ZJD 3.06 Å, Cryo-EM structure of kisspeptin receptor bound to KP-10
8ZJE 3.07 Å, Cryo-EM structure of kisspeptin receptor bound to TAK-448
7YQE 3.5 Å, Structure of human SRC regulatory domains in complex with the C-terminal PRRP motifs of…

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