Crystal structure of human serum albumin in complex with ALB8(VHH) domain of ozoralizumab. Determined by X-ray diffraction at 2.05 Å resolution. Released 4 Sept 2024.
Explore 8Z8V in 3D Show helices and sheets RCSB PDB PDBe
8Z8V contains 45 α-helices and 11 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 6-30 | 25 | |
| α-helix | 36-55 | 20 | |
| α-helix | 66-75 | 10 | |
| α-helix | 89-91 | 3 | |
| α-helix | 97-104 | 8 | |
| α-helix | 113-115 | 3 | |
| α-helix | 120-129 | 10 | |
| α-helix | 131-145 | 15 | |
| α-helix | 151-168 | 18 | |
| α-helix | 174-201 | 28 | |
| α-helix | 202-206 | 5 | |
| α-helix | 208-222 | 15 | |
| α-helix | 228-246 | 19 | |
| α-helix | 250-265 | 16 | |
| α-helix | 268-270 | 3 | |
| α-helix | 278-280 | 3 | |
| α-helix | 283-292 | 10 | |
| α-helix | 293-299 | 7 | |
| α-helix | 302-304 | 3 | |
| α-helix | 306 | 1 | |
| α-helix | 307-311 | 5 | |
| α-helix | 315-321 | 7 | |
| α-helix | 323-336 | 14 | |
| α-helix | 343-360 | 18 | |
| α-helix | 366-370 | 5 | |
| α-helix | 373-398 | 26 | |
| α-helix | 400-414 | 15 | |
| α-helix | 420-431 | 12 | |
| α-helix | 433-437 | 5 | |
| α-helix | 445-446 | 2 | |
| α-helix | 447-451 | 5 | |
| α-helix | 452-465 | 14 | |
| α-helix | 471-479 | 9 | |
| α-helix | 481-483 | 3 | |
| α-helix | 484-490 | 7 | |
| α-helix | 491-493 | 3 | |
| α-helix | 497-503 | 7 | |
| α-helix | 504-507 | 4 | |
| α-helix | 511-515 | 5 | |
| α-helix | 518-535 | 18 | |
| α-helix | 541-560 | 20 | |
| α-helix | 564-582 | 19 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 3-7 | 5 | 1 |
| β-strand | 11-12 | 2 | 2 |
| β-strand | 18-25 | 8 | 1 |
| α-helix | 29-31 | 3 | |
| β-strand | 34-39 | 6 | 3 |
| β-strand | 46-51 | 6 | 3 |
| β-strand | 58-60 | 3 | 3 |
| α-helix | 62-64 | 3 | |
| β-strand | 68-73 | 6 | 1 |
| β-strand | 78-83 | 6 | 1 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-98 | 7 | 3 |
| β-strand | 109-111 | 3 | 3 |
| β-strand | 112-113 | 2 | 2 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Serum albumin | A | protein | 585 | Homo sapiens | P02768 (AlphaFold model) |
| ALB8:VHH | B | protein | 123 | Homo sapiens |
>8Z8V_1 Serum albumin (chains A) DAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAE NCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEV DVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLP KLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTK VHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPA DLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKC CAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVST PTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTES LVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKAT KEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL
>8Z8V_2 ALB8:VHH (chains B) EVQLVESGGGLVQPGNSLRLSCAASGFTFSSFGMSWVRQAPGKGLEWVSSISGSGSDTLY ADSVKGRFTISRDNAKTTLYLQMNSLRPEDTAVYYCTIGGSLSRSSQGTLVTVSSTSHHH HHH
Structural design of the anti-TNF alpha therapeutic NANOBODY® compound, ozoralizumab, to support its potent and sustained clinical efficacy. Mima, M., Mishima-Tsumagari, C., Nakano, K. et al. Biochem Biophys Res Commun (2024) 734:150454-150454. DOI 10.1016/j.bbrc.2024.150454 · PubMed
Other PDB entries of the same protein (UniProt P02768 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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