9D3M: Two HMGN2s

Two HMGN2s in complex with the nucleosome. Determined by electron microscopy at 2.9 Å resolution. Released 13 Aug 2025.

Method: Electron microscopy
Resolution: 2.9 Å
Organisms: Xenopus borealis, Homo sapiens
Chains: 12
Atoms: 10,203
Mol. weight: 149 kDa
Released: 13 Aug 2025

Explore 9D3M in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9D3M contains 37 α-helices and 20 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 5 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	41-42	2
α-helix	45-56	12
α-helix	64-76	13
β-strand	83-84	2	1
α-helix	86-113	28
β-strand	118-119	2	2
α-helix	121-131	11

Chain B: 4 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	25-28	4
α-helix	31-40	10
β-strand	45-46	2	2
α-helix	50-75	26
β-strand	80-81	2	1
α-helix	83-93	11
β-strand	96-98	3	3

Chain C: 6 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	17-21	5
α-helix	27-36	10
β-strand	42-43	2	4
α-helix	46-72	27
β-strand	77-78	2	5
α-helix	80-89	10
α-helix	91-96	6
β-strand	101-102	2	6
α-helix	113-115	3

Chain D: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	38-48	11
β-strand	53-54	2	5
α-helix	56-83	28
β-strand	88-89	2	4
α-helix	91-101	11
α-helix	104-122	19

Chain E: 5 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	41-42	2
α-helix	45-55	11
α-helix	64-75	12
β-strand	83-84	2	7
α-helix	86-113	28
β-strand	118-119	2	8
α-helix	121-131	11

Chain F: 4 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	26-28	3
α-helix	31-40	10
β-strand	45-46	2	8
α-helix	50-75	26
β-strand	80-81	2	7
α-helix	83-93	11
β-strand	97-98	2	6

Chain G: 5 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	17-21	5
α-helix	27-36	10
β-strand	42-43	2	9
α-helix	46-72	27
β-strand	77-78	2	10
α-helix	80-89	10
α-helix	91-96	6
β-strand	100-102	3	3

Chain H: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	38-48	11
β-strand	53-54	2	10
α-helix	56-83	28
β-strand	88-89	2	9
α-helix	91-101	11
α-helix	105-122	18

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
5S rDNA (noncoding strand)	I	DNA	101	Xenopus borealis
5S rDNA (coding strand)	J	DNA	101	Xenopus borealis
Histone H3.2	A, E	protein	99	Homo sapiens	Q71DI3 (AlphaFold model)
Histone H4	B, F	protein	82	Homo sapiens	P62805 (AlphaFold model)
Histone H2A type 2-A	C, G	protein	103	Homo sapiens	Q6FI13 (AlphaFold model)
Histone H2B type 1-M	D, H	protein	93	Homo sapiens	Q99879 (AlphaFold model)
Non-histone chromosomal protein HMG-17	K, L	protein	6	Homo sapiens

Sequence of entity 1 (I), FASTA

>9D3M_1 5S rDNA (noncoding strand) (chains I)
AAGACCCTGGCATGGGGAGGAGCTGGGCCCCCCCCAGAAGGCAGCACAAGGGGAGGAAAA
GTCAGCCTTGTGCTCGCCTACGGCCATACCACCCTGAAAGT

Sequence of entity 2 (J), FASTA

>9D3M_2 5S rDNA (coding strand) (chains J)
ACTTTCAGGGTGGTATGGCCGTAGGCGAGCACAAGGCTGACTTTTCCTCCCCTTGTGCTG
CCTTCTGGGGGGGGCCCAGCTCCTCCCCATGCCAGGGTCTT

Sequence of entity 3 (A, E), FASTA

>9D3M_3 Histone H3.2 (chains A, E)
KPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEAS
EAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Sequence of entity 4 (B, F), FASTA

>9D3M_4 Histone H4 (chains B, F)
VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKT
VTAMDVVYALKRQGRTLYGFGG

Sequence of entity 5 (C, G), FASTA

>9D3M_5 Histone H2A type 2-A (chains C, G)
KSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYMAAVLEYLTAEILELAGNAARDNK
KTRIIPRHLQLAIRNDEELNKLLGKVTIAQGGVLPNIQAVLLP

Sequence of entity 6 (D, H), FASTA

>9D3M_6 Histone H2B type 1-M (chains D, H)
RSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTIT
SREIQTAVRLLLPGELAKHAVSEGTKAVTKYTS

Sequence of entity 7 (K, L), FASTA

>9D3M_7 Non-histone chromosomal protein HMG-17 (chains K, L)
RRSARL

Primary citation

Structural basis of nucleosome recognition by the conserved Dsup and HMGN nucleosome-binding motif. Alegrio-Louro, J., Cruz-Becerra, G., Kassavetis, G.A. et al. Genes Dev (2025) 39:1155-1161. DOI 10.1101/gad.352720.125 · PubMed

Other PDB entries of the same protein (UniProt Q71DI3 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2X4W 1.5 Å, Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1.
4MZG 1.7 Å, Crystal structure of human Spindlin1 bound to histone H3K4me3 peptide
2X4Y 1.7 Å, Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1.
2X4X 1.85 Å, Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1.
4OUC 1.9 Å, Structure of human haspin in complex with histone H3 substrate
5VAC 1.95 Å, Crystal Structure of ATXR5 SET domain in complex with K36me3 histone H3 peptide
6ACE 1.98 Å, histone lysine desuccinylase Sirt5 in complex with succinyl peptide H3K122
7UVA 1.98 Å, Crystal structure of KDM2A histone demethylase catalytic domain in complex with an H3C36…
5B0Z 1.99 Å, The crystal structure of the nucleosome containing H3.2, at 1.98 A resolution
3R93 2.06 Å, Crystal structure of the chromo domain of M-phase phosphoprotein 8 bound to H3K9Me3…
4MZF 2.1 Å, Crystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2a) peptide
4MZH 2.2 Å, Crystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2s) peptide

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