9D3N: Histone H3.2

167-bp 5S rDNA nucleosome cross-linked with glutaraldehyde. Determined by electron microscopy at 3.0 Å resolution. Released 13 Aug 2025.

Method: Electron microscopy
Resolution: 3.0 Å
Organisms: Homo sapiens, Xenopus borealis
Chains: 10
Atoms: 9,401
Mol. weight: 137.56 kDa
Released: 13 Aug 2025

Explore 9D3N in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9D3N contains 33 α-helices and 20 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	45-56	12
α-helix	64-78	15
β-strand	83-84	2	1
α-helix	86-113	28
β-strand	118-119	2	2
α-helix	121-131	11

Chain B: 3 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	31-41	11
β-strand	45-46	2	2
α-helix	50-75	26
β-strand	80-81	2	1
α-helix	83-91	9
β-strand	96-98	3	3

Chain C: 5 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	17-20	4
α-helix	27-36	10
β-strand	42-43	2	4
α-helix	47-72	26
β-strand	78	1	5
α-helix	80-89	10
α-helix	91-96	6
β-strand	100-102	3	6

Chain D: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	38-48	11
β-strand	54	1	5
α-helix	56-83	28
β-strand	88-89	2	4
α-helix	91-101	11
α-helix	105-123	19

Chain E: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	46-56	11
α-helix	64-75	12
β-strand	83	1	7
α-helix	88-113	26
β-strand	118-119	2	8
α-helix	121-131	11

Chain F: 4 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	26-28	3
α-helix	31-40	10
β-strand	45-46	2	8
α-helix	50-75	26
β-strand	80	1	7
α-helix	83-91	9
β-strand	96-98	3	6

Chain G: 5 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	19-21	3
α-helix	27-36	10
β-strand	42-43	2	9
α-helix	47-72	26
β-strand	77-78	2	10
α-helix	80-87	8
α-helix	91-96	6
β-strand	100-102	3	3

Chain H: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	38-48	11
β-strand	53-54	2	10
α-helix	59-83	25
β-strand	88-89	2	9
α-helix	91-101	11
α-helix	106-121	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Histone H3.2	A, E	protein	90	Homo sapiens	Q71DI3 (AlphaFold model)
Histone H4	B, F	protein	79	Homo sapiens	P62805 (AlphaFold model)
Histone H2A type 2-A	C	protein	91	Homo sapiens	Q6FI13 (AlphaFold model)
Histone H2B type 1-M	D, H	protein	89	Homo sapiens	Q99879 (AlphaFold model)
Histone H2A type 2-A	G	protein	91	Homo sapiens	Q6FI13 (AlphaFold model)
5S rDNA (noncoding strand)	I	DNA	96	Xenopus borealis
5S rDNA (coding strand)	J	DNA	96	Xenopus borealis

Sequence of entity 1 (A, E), FASTA

>9D3N_1 Histone H3.2 (chains A, E)
GTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGL
FEDTNLCAIHAKRVTIMPKDIQLARRIRGE

Sequence of entity 2 (B, F), FASTA

>9D3N_2 Histone H4 (chains B, F)
DNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTA
MDVVYALKRQGRTLYGFGG

Sequence of entity 3 (C), FASTA

>9D3N_3 Histone H2A type 2-A (chains C)
SRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYMAAVLEYLTAEILELAGNAARDNKK
TRIIPRHLQLAIRNDEELNKLLGKVTIAQGG

Sequence of entity 4 (D, H), FASTA

>9D3N_4 Histone H2B type 1-M (chains D, H)
SYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQ
TAVRLLLPGELAKHAVSEGTKAVTKYTSS

Sequence of entity 5 (G), FASTA

>9D3N_5 Histone H2A type 2-A (chains G)
SSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYMAAVLEYLTAEILELAGNAARDNKKTR
IIPRHLQLAIRNDEELNKLLGKVTIAQGGVL

Sequence of entity 6 (I), FASTA

>9D3N_6 5S rDNA (noncoding strand) (chains I)
GACCCTGGCATGGGGAGGAGCTGGGCCCCCCCCAGAAGGCAGCACAAGGGGAGGAAAAGT
CAGCCTTGTGCTCGCCTACGGCCATACCACCCTGAA

Sequence of entity 7 (J), FASTA

>9D3N_7 5S rDNA (coding strand) (chains J)
TTCAGGGTGGTATGGCCGTAGGCGAGCACAAGGCTGACTTTTCCTCCCCTTGTGCTGCCT
TCTGGGGGGGGCCCAGCTCCTCCCCATGCCAGGGTC

Primary citation

Structural basis of nucleosome recognition by the conserved Dsup and HMGN nucleosome-binding motif. Alegrio-Louro, J., Cruz-Becerra, G., Kassavetis, G.A. et al. Genes Dev (2025) 39:1155-1161. DOI 10.1101/gad.352720.125 · PubMed

Other PDB entries of the same protein (UniProt Q71DI3 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2X4W 1.5 Å, Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1.
4MZG 1.7 Å, Crystal structure of human Spindlin1 bound to histone H3K4me3 peptide
2X4Y 1.7 Å, Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1.
2X4X 1.85 Å, Molecular basis of Histone H3K36me3 recognition by the PWWP domain of BRPF1.
4OUC 1.9 Å, Structure of human haspin in complex with histone H3 substrate
5VAC 1.95 Å, Crystal Structure of ATXR5 SET domain in complex with K36me3 histone H3 peptide
6ACE 1.98 Å, histone lysine desuccinylase Sirt5 in complex with succinyl peptide H3K122
7UVA 1.98 Å, Crystal structure of KDM2A histone demethylase catalytic domain in complex with an H3C36…
5B0Z 1.99 Å, The crystal structure of the nucleosome containing H3.2, at 1.98 A resolution
3R93 2.06 Å, Crystal structure of the chromo domain of M-phase phosphoprotein 8 bound to H3K9Me3…
4MZF 2.1 Å, Crystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2a) peptide
4MZH 2.2 Å, Crystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2s) peptide

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