9DMH: Human muscle nAChR ACh-bound state
Human muscle nAChR ACh-bound state. Determined by electron microscopy at 2.06 Å resolution. Released 9 Apr 2025.
- Method
- Electron microscopy
- Resolution
- 2.06 Å
- Organism
- Homo sapiens
- Chains
- 5
- Atoms
- 16,919
- Mol. weight
- 281.71 kDa
- Ligands
- POV, ACH
- Released
- 9 Apr 2025
Explore 9DMH in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
9DMH contains 84 α-helices and 82 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 16 helices, 14 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-11 | 10 | |
| β-strand | 29-44 | 16 | 1 |
| β-strand | 49-61 | 13 | 1 |
| α-helix | 69-72 | 4 | |
| β-strand | 77-80 | 4 | 1 |
| α-helix | 82-84 | 3 | |
| β-strand | 90-92 | 3 | 2 |
| β-strand | 95 | 1 | 1 |
| β-strand | 101 | 1 | 2 |
| β-strand | 108-111 | 4 | 1 |
| β-strand | 115-118 | 4 | 1 |
| β-strand | 121-127 | 7 | 1 |
| β-strand | 139-148 | 10 | 2 |
| β-strand | 156-160 | 5 | 1 |
| β-strand | 166 | 1 | 1 |
| α-helix | 170-173 | 4 | |
| β-strand | 176-188 | 13 | 2 |
| α-helix | 197 | 1 | |
| β-strand | 198-209 | 12 | 2 |
| α-helix | 212-214 | 3 | |
| α-helix | 215-219 | 5 | |
| α-helix | 220-229 | 10 | |
| α-helix | 230-233 | 4 | |
| α-helix | 237-239 | 3 | |
| α-helix | 242-262 | 21 | |
| α-helix | 273-299 | 27 | |
| α-helix | 310-314 | 5 | |
| α-helix | 315-319 | 5 | |
| α-helix | 320-323 | 4 | |
| α-helix | 370-435 | 66 | |
Chain B: 16 helices, 19 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-12 | 10 | |
| β-strand | 29-44 | 16 | 12 |
| β-strand | 49-61 | 13 | 12 |
| α-helix | 63-65 | 3 | |
| α-helix | 69-71 | 3 | |
| β-strand | 77-81 | 5 | 12 |
| α-helix | 82-84 | 3 | |
| β-strand | 90-92 | 3 | 13 |
| β-strand | 95 | 1 | 12 |
| α-helix | 106 | 1 | |
| β-strand | 107-111 | 5 | 12 |
| β-strand | 115-118 | 4 | 12 |
| β-strand | 121-127 | 7 | 12 |
| β-strand | 139-148 | 10 | 13 |
| β-strand | 156-160 | 5 | 12 |
| β-strand | 162 | 1 | 14 |
| β-strand | 168 | 1 | 14 |
| β-strand | 171 | 1 | 15 |
| β-strand | 173 | 1 | 12 |
| α-helix | 176-178 | 3 | |
| β-strand | 180 | 1 | 12 |
| β-strand | 185-189 | 5 | 13 |
| β-strand | 191 | 1 | 15 |
| β-strand | 192-197 | 6 | 13 |
| β-strand | 207-218 | 12 | 13 |
| α-helix | 221-223 | 3 | |
| α-helix | 224-228 | 5 | |
| α-helix | 229-236 | 8 | |
| α-helix | 237-243 | 7 | |
| α-helix | 252-271 | 20 | |
| α-helix | 283-309 | 27 | |
| α-helix | 320-323 | 4 | |
| α-helix | 324-329 | 6 | |
| α-helix | 330-333 | 4 | |
| α-helix | 398-458 | 61 | |
Chain C: 17 helices, 13 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-11 | 10 | |
| α-helix | 28 | 1 | |
| β-strand | 29-44 | 16 | 3 |
| β-strand | 49-61 | 13 | 3 |
| α-helix | 69-72 | 4 | |
| β-strand | 77-81 | 5 | 3 |
| α-helix | 82-84 | 3 | |
| β-strand | 90-92 | 3 | 4 |
| β-strand | 95 | 1 | 3 |
| α-helix | 106 | 1 | |
| β-strand | 107-111 | 5 | 3 |
| β-strand | 115-118 | 4 | 3 |
| β-strand | 121-127 | 7 | 3 |
| β-strand | 139-148 | 10 | 4 |
| β-strand | 156-160 | 5 | 3 |
| β-strand | 166 | 1 | 3 |
| α-helix | 170-173 | 4 | |
| β-strand | 176-188 | 13 | 4 |
| β-strand | 198-209 | 12 | 4 |
| α-helix | 212-214 | 3 | |
| α-helix | 215-219 | 5 | |
| α-helix | 220-228 | 9 | |
| α-helix | 229-234 | 6 | |
| α-helix | 237-239 | 3 | |
| α-helix | 242-263 | 22 | |
| α-helix | 273-299 | 27 | |
| α-helix | 310-313 | 4 | |
| α-helix | 314-319 | 6 | |
| α-helix | 320-323 | 4 | |
| α-helix | 370-435 | 66 | |
Chain D: 19 helices, 19 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-8 | 7 | |
| α-helix | 9-14 | 6 | |
| β-strand | 31-46 | 16 | 8 |
| β-strand | 51-63 | 13 | 8 |
| α-helix | 65-67 | 3 | |
| α-helix | 71-74 | 4 | |
| β-strand | 79-82 | 4 | 8 |
| α-helix | 84-86 | 3 | |
| β-strand | 92-94 | 3 | 9 |
| β-strand | 97 | 1 | 8 |
| α-helix | 108-109 | 2 | |
| β-strand | 110-113 | 4 | 8 |
| β-strand | 117-120 | 4 | 8 |
| β-strand | 123-129 | 7 | 8 |
| α-helix | 131-133 | 3 | |
| β-strand | 141-150 | 10 | 9 |
| β-strand | 158-162 | 5 | 8 |
| β-strand | 164-167 | 4 | 10 |
| β-strand | 170-173 | 4 | 10 |
| β-strand | 176 | 1 | 11 |
| β-strand | 178 | 1 | 8 |
| β-strand | 185 | 1 | 8 |
| β-strand | 190-194 | 5 | 9 |
| β-strand | 196 | 1 | 11 |
| β-strand | 197-201 | 5 | 9 |
| α-helix | 210-212 | 3 | |
| β-strand | 213-223 | 11 | 9 |
| α-helix | 226-228 | 3 | |
| α-helix | 229-233 | 5 | |
| α-helix | 234-241 | 8 | |
| α-helix | 242-248 | 7 | |
| α-helix | 251-253 | 3 | |
| α-helix | 256-275 | 20 | |
| α-helix | 287-313 | 27 | |
| α-helix | 324-328 | 5 | |
| α-helix | 329-333 | 5 | |
| α-helix | 334-337 | 4 | |
| α-helix | 409-472 | 64 | |
Chain E: 16 helices, 17 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-11 | 10 | |
| α-helix | 20-21 | 2 | |
| β-strand | 29-44 | 16 | 5 |
| β-strand | 49-66 | 18 | 5 |
| α-helix | 69-72 | 4 | |
| β-strand | 77-81 | 5 | 5 |
| α-helix | 82-84 | 3 | |
| β-strand | 90-92 | 3 | 6 |
| β-strand | 95 | 1 | 5 |
| β-strand | 107-111 | 5 | 5 |
| β-strand | 113-118 | 6 | 5 |
| β-strand | 121-127 | 7 | 5 |
| β-strand | 139-148 | 10 | 6 |
| β-strand | 156-160 | 5 | 5 |
| β-strand | 171 | 1 | 7 |
| β-strand | 173 | 1 | 5 |
| β-strand | 180 | 1 | 5 |
| β-strand | 185-189 | 5 | 6 |
| β-strand | 191 | 1 | 7 |
| β-strand | 192-195 | 4 | 6 |
| α-helix | 196-197 | 2 | |
| β-strand | 211-220 | 10 | 6 |
| α-helix | 223-225 | 3 | |
| α-helix | 226-230 | 5 | |
| α-helix | 231-242 | 12 | |
| α-helix | 243-245 | 3 | |
| α-helix | 248-250 | 3 | |
| α-helix | 253-272 | 20 | |
| α-helix | 284-310 | 27 | |
| α-helix | 321-324 | 4 | |
| α-helix | 325-330 | 6 | |
| α-helix | 331-334 | 4 | |
| α-helix | 408-468 | 61 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Acetylcholine receptor subunit alpha | A, C | protein | 457 | Homo sapiens | P02708 (AlphaFold model) |
| Acetylcholine receptor subunit beta | E | protein | 503 | Homo sapiens | P11230 (AlphaFold model) |
| Acetylcholine receptor subunit delta | D | protein | 517 | Homo sapiens | Q07001 (AlphaFold model) |
| Acetylcholine receptor subunit epsilon | B | protein | 493 | Homo sapiens | Q04844 (AlphaFold model) |
Sequence of entity 1 (A, C), FASTA
>9DMH_1 Acetylcholine receptor subunit alpha (chains A, C)
MEPWPLLLLFSLCSAGLVLGSEHETRLVAKLFKDYSSVVRPVEDHRQVVEVTVGLQLIQL
INVDEVNQIVTTNVRLKQQWVDYNLKWNPDDYGGVKKIHIPSEKIWRPDLVLYNNADGDF
AIVKFTKVLLQYTGHITWTPPAIFKSYCEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINP
ESDQPDLSNFMESGEWVIKESRGWKHSVTYSCCPDTPYLDITYHFVMQRLPLYFIVNVII
PCLLFSFLTGLVFYLPTDSGEKMTLSISVLLSLTVFLLVIVELIPSTSSAVPLIGKYMLF
TMVFVIASIIITVIVINTHHRSPSTHVMPNWVRKVFIDTIPNIMFFSTMKRPSREKQDKK
IFTEDIDISDISGKPGPPPMGFHSPLIKHPEVKSAIEGIKYIAETMKSDQESNNAAAEWK
YVAMVMDHILLGVFMLVCIIGTLAVFAGRLIELNQQG
Sequence of entity 2 (E), FASTA
>9DMH_2 Acetylcholine receptor subunit beta (chains E)
MTPGALLMLLGALGAPLAPGVRGSEAEGRLREKLFSGYDSSVRPAREVGDRVRVSVGLIL
AQLISLNEKDEEMSTKVYLDLEWTDYRLSWDPAEHDGIDSLRITAESVWLPDVVLLNNND
GNFDVALDISVVVSSDGSVRWQPPGIYRSSCSIQVTYFPFDWQNCTMVFSSYSYDSSEVS
LQTGLGPDGQGHQEIHIHEGTFIENGQWEIIHKPSRLIQPPGDPRGGREGQRQEVIFYLI
IRRKPLFYLVNVIAPCILITLLAIFVFYLPPDAGEKMGLSIFALLTLTVFLLLLADKVPE
TSLSVPIIIKYLMFTMVLVTFSVILSVVVLNLHHRSPHTHQMPLWVRQIFIHKLPLYLRL
KRPKPERDLMPEPPHCSSPGSGWGRGTDEYFIRKPPSDFLFPKPNRFQPELSAPDLRRFI
DGPNRAVALLPELREVVSSISYIARQLQEQEDHDALKEDWQFVAMVVDRLFLWTFIIFTS
VGTLVIFLDATYHLPPPDPFPSR
Sequence of entity 3 (D), FASTA
>9DMH_3 Acetylcholine receptor subunit delta (chains D)
MEGPVLTLGLLAALAVCGSWGLNEEERLIRHLFQEKGYNKELRPVAHKEESVDVALALTL
SNLISLKEVEETLTTNVWIEHGWTDNRLKWNAEEFGNISVLRLPPDMVWLPEIVLENNND
GSFQISYSCNVLVYHYGFVYWLPPAIFRSSCPISVTYFPFDWQNCSLKFSSLKYTAKEIT
LSLKQDAKENRTYPVEWIIIDPEGFTENGEWEIVHRPARVNVDPRAPLDSPSRQDITFYL
IIRRKPLFYIINILVPCVLISFMVNLVFYLPADSGEKTSVAISVLLAQSVFLLLISKRLP
ATSMAIPLIGKFLLFGMVLVTMVVVICVIVLNIHFRTPSTHVLSEGVKKLFLETLPELLH
MSRPAEDGPSPGALVRRSSSLGYISKAEEYFLLKSRSDLMFEKQSERHGLARRLTTARRP
PASSEQAQQELFNELKPAVDGANFIVNHMRDQNNYNEEKDSWNRVARTVDRLCLFVVTPV
MVVGTAWIFLQGVYNQPPPQPFPGDPYSYNVQDKRFI
Sequence of entity 4 (B), FASTA
>9DMH_4 Acetylcholine receptor subunit epsilon (chains B)
MARAPLGVLLLLGLLGRGVGKNEELRLYHHLFNNYDPGSRPVREPEDTVTISLKVTLTNL
ISLNEKEETLTTSVWIGIDWQDYRLNYSKDDFGGIETLRVPSELVWLPEIVLENNIDGQF
GVAYDANVLVYEGGSVTWLPPAIYRSVCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFTF
AVDNDGKTINKIDIDTEAYTENGEWAIDFCPGVIRRHHGGATDGPGETDVIYSLIIRRKP
LFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKIPETSLS
VPLLGRFLIFVMVVATLIVMNCVIVLNVSQRTPTTHAMSPRLRHVLLELLPRLLGSPPPP
EAPRAASPPRRASSVGLLLRAEELILKKPRSELVFEGQRHRQGTWTAAFCQSLGAAAPEV
RCCVDAVNFVAESTRDQEATGEEVSDWVRMGNALDNICFWAALVLFSVGSSLIFLGAYFN
RVPDLPYAPCIQP
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| POV | (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl… | C42 H82 N O8 P | 3 |
| ACH | Acetylcholine | C7 H16 N O2 | 2 |
Primary citation
Autoimmune mechanisms elucidated through muscle acetylcholine receptor structures. Li, H., Pham, M.C., Teng, J. et al. Cell (2025) 188:2390-2406.e20. DOI 10.1016/j.cell.2025.03.004 · PubMed
Other PDB entries of the same protein (UniProt P02708 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 9DMS 1.92 Å, Human muscle nAChR with fab6-bound
- 9DMG 2.05 Å, Human muscle nAChR apo state
- 9DMQ 2.06 Å, Human muscle nAChR with fab3-bound
- 9DMV 2.13 Å, Human muscle nAChR with fab9-bound
- 9DMT 2.18 Å, Human muscle nAChR with fab7-bound
- 9DMJ 2.19 Å, Human muscle nAChR with two fab1b-bound
- 9YER 2.19 Å, Human muscle nAChR SCCMS eT264P ACh bound
- 9YE8 2.22 Å, Human muscle nAChR SCCMS eL269F ACh bound
- 4ZJS 2.23 Å, Crystal structure of a chimeric acetylcholine binding protein from Aplysia Californica…
- 9DML 2.24 Å, Human muscle nAChR with fab2-bound
- 9YEI 2.36 Å, Human muscle nAChR SCCMS eL269F ACh, Reboxetine bound (lower 1)
- 9YET 2.36 Å, Human muscle nAChR SCCMS eT264P ACh, Reboxetine bound
Browse structure collections
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