9E2W: DNA replication licensing factor MCM2

Cryo-EM structure of yeast CMG helicase stalled at G4-containing DNA template, state 1. Determined by electron microscopy at 3.3 Å resolution. Released 26 Mar 2025.

Method: Electron microscopy
Resolution: 3.3 Å
Organisms: Saccharomyces cerevisiae W303, synthetic construct
Chains: 15
Atoms: 48,591
Mol. weight: 965.6 kDa
Ligands: ATP, ZN, MG, ADP
Released: 26 Mar 2025

Explore 9E2W in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9E2W contains 312 α-helices and 229 β-strands across 13 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 2: 33 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	176-178	3
α-helix	183-187	5
α-helix	194-199	6
α-helix	201-217	17
β-strand	219	1	1
β-strand	225	1	1
α-helix	226-236	11
β-strand	241-244	4	2
α-helix	246-252	7
α-helix	254-262	9
α-helix	264-282	19
α-helix	286-288	3
β-strand	294-297	4	2
β-strand	304	1	3
α-helix	311-313	3
β-strand	317-327	11	3
β-strand	331-334	4	3
β-strand	336-337	2	4
β-strand	339-341	3	5
β-strand	347	1	5
α-helix	348-350	3
β-strand	351-352	2	4
β-strand	373-375	3	5
β-strand	382-391	10	3
α-helix	398	1
β-strand	405-411	7	3
β-strand	423-433	11	3
α-helix	436-442	7
β-strand	446	1	6
β-strand	448-458	11	3
α-helix	463-465	3
α-helix	478-489	12
α-helix	493-499	7
α-helix	509-520	12
β-strand	525-527	3	7
β-strand	531-533	3	7
β-strand	539-543	5	8
α-helix	550-559	10
β-strand	563-567	5	8
β-strand	571	1	9
β-strand	578-582	5	10
β-strand	589-593	5	10
α-helix	595-598	4
β-strand	603-607	5	8
α-helix	609-611	3
α-helix	614-626	13
β-strand	628-633	6	11
β-strand	636-641	6	11
β-strand	645-650	6	8
α-helix	653-655	3
α-helix	663-666	4
α-helix	671-675	5
β-strand	679-683	5	8
α-helix	689-705	17
α-helix	740-755	16
α-helix	760-773	14
β-strand	777	1	12
α-helix	783-797	15
α-helix	803-804	2
α-helix	807-823	17
β-strand	828	1	12
α-helix	831-847	17
α-helix	851-860	10
α-helix	861-863	3

Chain 3: 30 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	20-34	15
α-helix	36-51	16
β-strand	95-99	5	13
α-helix	100-106	7
α-helix	108-116	9
α-helix	118-136	19
α-helix	144-148	5
β-strand	154-158	5	13
β-strand	165-166	2	14
α-helix	173-175	3
β-strand	179-189	11	14
α-helix	190-192	3
β-strand	193-203	11	14
β-strand	208-213	6	14
α-helix	230-232	3
β-strand	234	1	15
β-strand	240	1	15
β-strand	242-243	2	14
β-strand	249-259	11	14
α-helix	262-264	3
α-helix	270-272	3
β-strand	273-279	7	14
α-helix	280-282	3
β-strand	291-301	11	14
β-strand	318-328	11	14
α-helix	340-343	4
α-helix	344-354	11
α-helix	359-365	7
α-helix	375-386	12
β-strand	391-392	2	16
β-strand	398-399	2	16
β-strand	405-409	5	17
α-helix	415-425	11
β-strand	429-433	5	17
β-strand	437	1	18
α-helix	439-443	5
β-strand	444-448	5	19
β-strand	455-459	5	19
α-helix	461-464	4
β-strand	469-473	5	17
α-helix	475-477	3
α-helix	480-492	13
β-strand	494-499	6	20
β-strand	502-507	6	20
β-strand	511-516	6	17
α-helix	529-532	4
α-helix	537-541	5
β-strand	545-549	5	17
α-helix	555-569	15
β-strand	579	1	21
α-helix	580-582	3
α-helix	650-652	3
α-helix	653-666	14
β-strand	670	1	22
α-helix	673-687	15
α-helix	699-715	17
β-strand	720	1	22
α-helix	723-738	16

Chain 4: 28 helices, 34 β-strands

Element	Residues	Length	Sheet
β-strand	341-344	4	23
β-strand	350	1	23
α-helix	351-363	13
β-strand	366	1	24
α-helix	367-373	7
α-helix	377-379	3
β-strand	388	1	24
α-helix	390-400	11
β-strand	404-408	5	25
α-helix	409-414	6
α-helix	419-427	9
α-helix	429-451	23
α-helix	457-461	5
β-strand	466-470	5	25
α-helix	483-485	3
β-strand	489-499	11	26
α-helix	500-502	3
β-strand	503-513	11	26
β-strand	519-523	5	26
β-strand	524-525	2	27
β-strand	528-529	2	27
β-strand	534	1	28
β-strand	543	1	28
β-strand	546-548	3	26
α-helix	550-552	3
β-strand	554-565	12	26
α-helix	567-569	3
α-helix	575-577	3
β-strand	578-584	7	26
α-helix	585-587	3
β-strand	596-606	11	26
β-strand	609	1	29
β-strand	617-618	2	29
β-strand	620	1	30
α-helix	621	1
β-strand	622-632	11	26
α-helix	667-678	12
α-helix	682-690	9
α-helix	698-709	12
β-strand	714-716	3	31
β-strand	720-722	3	31
β-strand	728-732	5	32
α-helix	739-748	10
β-strand	754-755	2	32
β-strand	768-772	5	33
β-strand	777-781	5	33
α-helix	784-787	4
β-strand	792-795	4	32
α-helix	803-815	13
β-strand	817-820	4	34
β-strand	827-830	4	34
β-strand	834-839	6	32
α-helix	852-856	5
α-helix	860-863	4
β-strand	868-872	5	32
α-helix	878-890	13
α-helix	908-921	14
β-strand	925-926	2	35
α-helix	928-944	17
α-helix	960-975	16
β-strand	980-981	2	35
α-helix	983-999	17

Chain 5: 34 helices, 25 β-strands

Element	Residues	Length	Sheet
α-helix	23-37	15
α-helix	45-55	11
β-strand	60-64	5	36
α-helix	65-71	7
α-helix	73-81	9
α-helix	83-102	20
α-helix	133-135	3
β-strand	136-140	5	36
α-helix	143-147	5
α-helix	148-150	3
α-helix	153-155	3
β-strand	159-169	11	37
α-helix	170-172	3
β-strand	173-183	11	37
β-strand	189-193	5	37
β-strand	241-259	19	37
α-helix	260-261	2
α-helix	262-264	3
α-helix	270-272	3
β-strand	273-279	7	37
α-helix	280-282	3
β-strand	291-301	11	37
β-strand	323	1	3
β-strand	325-335	11	37
α-helix	339-343	5
α-helix	351-361	11
α-helix	366-373	8
α-helix	382-393	12
β-strand	398-399	2	38
β-strand	405-406	2	38
β-strand	412-416	5	39
α-helix	422-432	11
β-strand	436-440	5	39
β-strand	444	1	11
α-helix	446-449	4
β-strand	453-455	3	40
β-strand	462-464	3	40
α-helix	468-471	4
β-strand	476-480	5	39
α-helix	482-484	3
α-helix	487-490	4
α-helix	491-493	3
α-helix	494-498	5
β-strand	501-506	6	18
β-strand	509-514	6	18
β-strand	518-523	6	39
α-helix	536-539	4
α-helix	544-549	6
β-strand	552-556	5	39
α-helix	562-577	16
α-helix	596-609	14
β-strand	611	1	21
β-strand	613-614	2	41
α-helix	616-636	21
α-helix	650-666	17
β-strand	671-672	2	41
α-helix	674-684	11
α-helix	685-689	5
α-helix	690-693	4

Chain 6: 35 helices, 34 β-strands

Element	Residues	Length	Sheet
β-strand	183-186	4	42
α-helix	188-190	3
α-helix	192-193	2
α-helix	195-210	16
β-strand	212	1	43
β-strand	223	1	43
α-helix	224-235	12
β-strand	239-243	5	44
α-helix	244-249	6
α-helix	250-253	4
α-helix	254-261	8
α-helix	263-281	19
α-helix	283-285	3
β-strand	287	1	45
α-helix	348	1
β-strand	349	1	45
α-helix	350	1
β-strand	353-357	5	44
β-strand	362-363	2	6
α-helix	365-367	3
α-helix	370-372	3
β-strand	376-386	11	6
α-helix	387-389	3
β-strand	390-400	11	6
β-strand	406-411	6	6
β-strand	433-435	3	6
β-strand	441-451	11	6
α-helix	458	1
α-helix	462-464	3
β-strand	465-471	7	6
α-helix	472-474	3
β-strand	483-494	12	6
α-helix	496-499	4
β-strand	501	1	46
β-strand	504	1	46
α-helix	524-525	2
β-strand	526	1	47
β-strand	527	1	27
β-strand	537	1	47
β-strand	539-550	12	6
α-helix	590-596	7
α-helix	599-610	12
α-helix	614-620	7
α-helix	630-641	12
β-strand	646-647	2	48
β-strand	653-654	2	48
β-strand	660-664	5	49
α-helix	670-678	9
β-strand	684-687	4	49
β-strand	700-703	4	50
β-strand	710-713	4	50
α-helix	716-719	4
β-strand	724-727	4	49
α-helix	730-732	3
α-helix	735-746	12
β-strand	749-754	6	9
β-strand	757-762	6	9
β-strand	766-771	6	49
α-helix	784-787	4
α-helix	792-797	6
β-strand	800-804	5	49
α-helix	810-826	17
α-helix	827-830	4
α-helix	837-849	13
β-strand	853-854	2	51
α-helix	856-873	18
α-helix	886-902	17
β-strand	907-908	2	51
α-helix	910-922	13

Chain 7: 31 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	7-8	2	14
α-helix	14-27	14
α-helix	62-71	10
β-strand	77-81	5	52
α-helix	82-95	14
α-helix	102-108	7
α-helix	110-123	14
α-helix	125-128	4
α-helix	134-136	3
α-helix	138-155	18
α-helix	194-197	4
β-strand	201-205	5	52
α-helix	206-208	3
α-helix	209-214	6
β-strand	224-225	2	30
α-helix	232-234	3
β-strand	238-248	11	30
α-helix	249-251	3
β-strand	252-262	11	30
β-strand	268-272	5	30
β-strand	277-278	2	29
α-helix	279-281	3
β-strand	283	1	53
α-helix	287-292	6
β-strand	297	1	53
β-strand	298-316	19	30
α-helix	319-321	3
β-strand	330-336	7	30
α-helix	337-339	3
β-strand	348-358	11	30
α-helix	363-368	6
β-strand	371	1	14
β-strand	373-383	11	30
α-helix	410-418	9
α-helix	426-437	12
β-strand	456-460	5	54
β-strand	462	1	55
β-strand	464	1	55
α-helix	466-476	11
β-strand	481-484	4	54
β-strand	520-524	5	54
α-helix	531-543	13
β-strand	545-547	3	56
β-strand	556-558	3	56
β-strand	562-567	6	54
α-helix	580-583	4
α-helix	588-593	6
β-strand	596-600	5	54
α-helix	606-622	17
α-helix	631-634	4
α-helix	635-647	13
β-strand	651	1	57
α-helix	654-675	22
α-helix	686-703	18
β-strand	707	1	57
α-helix	708-709	2
α-helix	710-729	20

Chain A: 9 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	4-22	19
α-helix	29-32	4
α-helix	34-59	26
α-helix	65-103	39
α-helix	120-122	3
α-helix	125-142	18
α-helix	145-147	3
α-helix	157-158	2
β-strand	162-167	6	58
β-strand	173-175	3	59
β-strand	180-182	3	59
β-strand	188-192	5	58
α-helix	197-202	6
β-strand	205-207	3	58

Chain B: 9 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	13-20	8
β-strand	24-29	6	60
α-helix	58-61	4
β-strand	69-72	4	60
α-helix	73-81	9
β-strand	85-87	3	60
α-helix	91-93	3
α-helix	95-107	13
α-helix	119-129	11
α-helix	138-159	22
α-helix	160-162	3
β-strand	169	1	61
α-helix	175-199	25

5 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA replication licensing factor MCM2	2	protein	868	Saccharomyces cerevisiae W303	P29469 (AlphaFold model)
DNA replication licensing factor MCM3	3	protein	971	Saccharomyces cerevisiae W303	P24279 (AlphaFold model)
DNA replication licensing factor MCM4	4	protein	933	Saccharomyces cerevisiae W303	P30665 (AlphaFold model)
Minichromosome maintenance protein 5	5	protein	775	Saccharomyces cerevisiae W303	P29496 (AlphaFold model)
DNA replication licensing factor MCM6	6	protein	1017	Saccharomyces cerevisiae W303	P53091
DNA replication licensing factor MCM7	7	protein	845	Saccharomyces cerevisiae W303	P38132
DNA replication complex GINS protein PSF1	A	protein	208	Saccharomyces cerevisiae W303	Q12488
DNA replication complex GINS protein PSF2	B	protein	213	Saccharomyces cerevisiae W303	P40359
DNA replication complex GINS protein PSF3	C	protein	217	Saccharomyces cerevisiae W303	Q12146
DNA replication complex GINS protein SLD5	D	protein	294	Saccharomyces cerevisiae W303	Q03406
Cell division control protein 45	E	protein	650	Saccharomyces cerevisiae W303	Q08032
Leading strand DNA template	F	DNA	48	synthetic construct

3 more molecules are not listed.

Sequence of entity 1 (2), FASTA

>9E2W_1 DNA replication licensing factor MCM2 (chains 2)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH

Sequence of entity 2 (3), FASTA

>9E2W_2 DNA replication licensing factor MCM3 (chains 3)
MEGSTGFDGDATTFFAPDAVFGDRVRRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQ
DDADERDLLGDDDGDDLEKEKKAASSTSLNILPHRIIISLDDLREFDRSFWSGILVEPAY
FIPPAEKALTDLADSMDDVPHPNASAVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLV
SVEGIVTKTSLVRPKLIRSVHYAAKTGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNK
LTTEYGYSTFIDHQRITVQEMPEMAPAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKS
LGAGGMNQSNSNTLIGFKTLILGNTVYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIF
DILSQSLAPSIYGHDHIKKAILLMLMGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLR
FVLNTASLAIATTGRGSSGVGLTAAVTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTD
VDRVAIHEVMEQQTVTIAKAGIHTTLNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLL
SRFDLLFVVTDDINEIRDRSISEHVLRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINP
EEHSNSGAGVENEGEDDEDHVFEKFNPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYV
QYAKERVIPQLTQEAINVIVKNYTDLRNDDNTKKSPITARTLETLIRLATAHAKVRLSKT
VNKVDAKVAANLLRFALLGEDIGNDIDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSG
SPIKSTPRRSTASSVNATPSSARRILRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQL
GLRVSPRRREHLHAPEEGSSGPLTEVGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTIST
GRLSLISGIIARLMQTEIFEEESYPVASLFERINEELPEEEKFSAQEYLAGLKIMSDRNN
LMVADDKVWRV

Sequence of entity 3 (4), FASTA

>9E2W_3 DNA replication licensing factor MCM4 (chains 4)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV

Sequence of entity 4 (5), FASTA

>9E2W_4 Minichromosome maintenance protein 5 (chains 5)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV

Sequence of entity 5 (6), FASTA

>9E2W_5 DNA replication licensing factor MCM6 (chains 6)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS

Sequence of entity 6 (7), FASTA

>9E2W_6 DNA replication licensing factor MCM7 (chains 7)
MSAALPSIQLPVDYNNLFNEITDFLVTFKQDTLSSDATRNENEDENLDAENIEQHLLEKG
PKYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRA
IDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDA
LREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLIT
VRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFM
STRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPY
TGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAP
EIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRG
VYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEV
MEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLM
LDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYV
VQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLV
RVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLS
NCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANVSAQDSDI
DLQDA

Sequence of entity 7 (A), FASTA

>9E2W_7 DNA replication complex GINS protein PSF1 (chains A)
MYGDLGNKLVLEAKRTKQLYARSNQDVNLPMYHEDIIRNILKEVSNLRKNTEYLKEQQQL
GMLDDKVAKCQYFVTLLCMERNKRCLLAYQRLRTDILDSMAWNNNGLDLMSSITFSQQDT
NNLSHQEQEYLKEYCDLITDLKSGDLVDIDLSGSLVPPSDVFIDVRVLKDAGEIQTEYGV
FNLIKDSQFFVRQSDVERLIQQGYLQKI

Sequence of entity 8 (B), FASTA

>9E2W_8 DNA replication complex GINS protein PSF2 (chains B)
MSLPAHLQQTFSPEEIQFIVENEPIKIFPRITTRQKIRGDDRGTGNHTRWQLITTDDKAL
NNMVAMRSTEVVLWIALLLKQQSKCSIVAPQWLTTKELDRKIQYEKTHPDRFSELPWNWL
VLARILFNKAKDDFHDPIHELRGKIQDLREIRQIKVLKGLKYLNESHLQLDNLSLLEINE
LRPFITEIMDKLREIHTASLTAGTENDEEEFNI

Sequence of entity 9 (C), FASTA

>9E2W_9 DNA replication complex GINS protein PSF3 (chains C)
MGSSHHHHHHSSGLVPRGSHMASMGYYDIDDVLADGTEFPCKFQYDIPGLGYLENNPGRP
ITKNTKLSLPLWLARILAIVGGDEALVDEEPVPFVELLPPDMFSTKVMNAIKTDPVALDL
HSINSHFFSLAIKWIMLFSEKELANVVSELLLQRAQELNHHASSLSIDLNADSTGKNSAN
TNIATSTFLLKLEEMEKEIYKKSHESYKDTKRWMFKK

Sequence of entity 10 (D), FASTA

>9E2W_10 DNA replication complex GINS protein SLD5 (chains D)
MDINIDDILAELDKETTAVDSTKITQGSSSTTHRDANTIVGSSLDLNDKTQIYVSPQQDF
SDLMKSWKNERCSPELLPYPHQLMKRLLNRISMQSQLIENISMGFLDMQNASNANPPMPN
ESKLPLLCMETELERLKFVIRSYIRCRLSKIDKFSLYLRQLNEDENSLISLTDLLSKDEI
KYHDTHSLIWLKLVNDSILKYMPEELQAINDTEGSVNMIDEPDWNKFVFIHVNGPPDGKW
NEDPLLQENEFGKPCYTVTIPDLKEEVELTIGSIYVMRYEVIRDLLRDDKVALI

Sequence of entity 11 (E), FASTA

>9E2W_11 Cell division control protein 45 (chains E)
MYYGISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFKKQLVQSQIVP
IFGYSELRRHYSQLDDNINSLLLVGFGGVIDLEAFLEIDPQEYVIDTDEKSGEQSFRRDI
YVLDAHRPWNLDNIFGSQIIQCFDDGTVDDTLGEQKEAYYKLLELDEESGDDELSGDEND
NNGGDDEATDADEVTDEDEEDEDETISNKRGNSSIGPNDLSKRKQRKKQIHEYEGVLEEY
YSQGTTVVNSISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYPLLQDEVK
RLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSLWNENGKKRLH
KMFARMGIPLSTAQETWLYMDHSIKRELGIIFDKNLDRYGLQDIIRDGFVRTLGYRGSIS
ASEFVEALTALLEVGNSTDKDSVKINNDNNDDTDGEEEEDNSAQKLTNLRKRWVSNFWLS
WDALDDRKVELLNRGIQLAQDLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDGPDLDLYR
NPLTLLRLGNWLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGLDTIHTKKP
ILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL

Sequence of entity 12 (F), FASTA

>9E2W_12 Leading strand DNA template (chains F)
TCGTGCTGAGTGATATCTGCTTTGGGTGGGTGGGTGGGTTGAGGCAAT

Ligands and cofactors

ID	Name	Formula	Copies
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	5
ZN	Zinc ion	Zn	5
MG	Magnesium ion	Mg	6
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	1

Primary citation

G-quadruplex-stalled eukaryotic replisome structure reveals helical inchworm DNA translocation. Batra, S., Allwein, B., Kumar, C. et al. Science (2025) 387:eadt1978-eadt1978. DOI 10.1126/science.adt1978 · PubMed

Other PDB entries of the same protein (UniProt P29469 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7W8G 2.52 Å, Cryo-EM structure of MCM double hexamer
28VY 2.7 Å, sCMGE assembled on ARS1 DNA with Sld2 and RPA
8RIF 2.79 Å, Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
7V3V 2.9 Å, Cryo-EM structure of MCM double hexamer bound with DDK in State I
9TH8 2.9 Å, Cryo-EM structure of MCM2-7 DH bound to Sld3-Sld7, Cdc45 and DNA
7P30 3.0 Å, 3.0 A resolution structure of a DNA-loaded MCM double hexamer
8KG6 3.07 Å, Yeast replisome in state I
9RHL 3.1 Å, Phospho-DH bound by Sld3-MBD on ARS1 DNA
7PMK 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
7PMN 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
7PT6 3.2 Å, Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III
7V3U 3.2 Å, Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD

Browse structure collections

About this viewer

MolViewer shows 9E2W directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.