9JR3: PTH-PTH1R-Gq

Cryo-EM structure of PTH-PTH1R-Gq (tilted state). Determined by electron microscopy at 2.8 Å resolution. Released 16 Oct 2024.

Method: Electron microscopy
Resolution: 2.8 Å
Organisms: Homo sapiens, Rattus rattus, Bos taurus
Chains: 6
Atoms: 9,455
Mol. weight: 169.42 kDa
Released: 16 Oct 2024

Explore 9JR3 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9JR3 contains 36 α-helices and 71 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 9 β-strands

Element	Residues	Length	Sheet
α-helix	7-30	24
β-strand	34-39	6	4
β-strand	69	1	5
β-strand	72-76	5	4
β-strand	79-85	7	4
β-strand	86	1	5
α-helix	97-100	4
β-strand	105-111	7	4
α-helix	118-129	12
α-helix	132-134	3
β-strand	139-144	6	4
α-helix	146-155	10
α-helix	160-163	4
α-helix	165-169	5
α-helix	184-201	18
β-strand	211	1	4
β-strand	214-215	2	4
α-helix	224-243	20

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	5-24	20
α-helix	30-33	4
β-strand	47-51	5	6
β-strand	58-63	6	7
β-strand	69-74	6	7
β-strand	78-83	6	7
β-strand	88-94	7	7
β-strand	100-105	6	8
β-strand	111-116	6	8
β-strand	121-125	5	8
α-helix	129-131	3
β-strand	134-139	6	8
β-strand	146-153	8	9
β-strand	156-161	6	9
β-strand	165-170	6	9
β-strand	175-181	7	9
β-strand	187-192	6	10
β-strand	198-203	6	10
β-strand	208-212	5	10
β-strand	218-222	5	10
β-strand	229-234	6	11
β-strand	240-245	6	11
β-strand	250-254	5	11
β-strand	260-264	5	11
β-strand	273-278	6	12
β-strand	284-289	6	12
β-strand	294-298	5	12
β-strand	304-308	5	12
β-strand	315-320	6	6
β-strand	327-331	5	6
β-strand	336-339	4	6

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	12-23	12
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3

Chain P: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	3-16	14
α-helix	18-33	16

Chain R: 16 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	34-53	20
β-strand	111-112	2	1
β-strand	117-118	2	1
β-strand	126-130	5	2
α-helix	131-132	2
β-strand	143-147	5	2
α-helix	155	1
β-strand	156	1	3
α-helix	157	1
β-strand	163	1	3
β-strand	166	1	2
α-helix	177-211	35
α-helix	213-215	3
α-helix	218-244	27
α-helix	279-311	33
α-helix	318-346	29
α-helix	361-390	30
α-helix	400-417	18
α-helix	419-422	4
α-helix	435-449	15
α-helix	451-456	6
α-helix	457-461	5
α-helix	464-481	18

Chain S: 2 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	13
β-strand	10-12	3	14
β-strand	18-25	8	13
α-helix	29-31	3
β-strand	33-39	7	14
β-strand	45-51	7	14
α-helix	53-55	3
β-strand	58-60	3	14
β-strand	69-73	5	13
β-strand	78-83	6	13
β-strand	92-99	8	14
β-strand	110	1	14
β-strand	113	1	13
β-strand	115-119	5	14
β-strand	128-129	2	15
β-strand	135-136	2	16
β-strand	143-146	4	17
β-strand	148-149	2	15
β-strand	154	1	18
β-strand	160	1	18
β-strand	162-167	6	19
β-strand	174-178	5	19
β-strand	182-183	2	19
β-strand	191-196	6	17
β-strand	199-204	6	17
β-strand	214-219	6	19
β-strand	227	1	19
β-strand	231-232	2	19
β-strand	233-234	2	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Parathyroid hormone/parathyroid hormone-related peptide receptor	R	protein	567	Homo sapiens	Q03431 (AlphaFold model)
Parathyroid hormone	P	protein	35	Homo sapiens	P01270 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1 (miniGq)	A	protein	245	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	344	Rattus rattus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	68	Bos taurus	P63212
scFv16	S	protein	260	Mus musculus

Sequence of entity 1 (R), FASTA

>9JR3_1 Parathyroid hormone/parathyroid hormone-related peptide receptor (chains R)
DADDVMTKEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKPRKDKASGK
LYPESEEDKEAPTGSRYRGRPCLPEWDHILCWPLGAPGEVVAVPCPDYIYDFNHKGHAYR
RCDRNGSWELVPGHNRTWANYSECVKFLTNETREREVFDRLGMIYTVGYSVSLASLTVAV
LILAYFRRLHCTRNYIHMHLFLSFMLRAVSIFVKDAVLYSGATLDEAERLTEEELRAIAQ
APPPPATAAAGYAGCRVAVTFFLYFLATNYYWILVEGLYLHSLIFMAFFSEKKYLWGFTV
FGWGLPAVFVAVWVSVRATLANTGCWDLSSGNKKWIIQVPILASIVLNFILFINIVRVLA
TKLRETNAGRCDTRQQYRKLLKSTLVLMPLFGVHYIVFMATPYTEVSGTLWQVQMHYEML
FNSFQGFFVAIIYCFCNGEVQAEIKKSWSRWTLALDFKRKARSGSSSYSYGPMVSHTSVT
NVGPRVGLGLPLSPRLLPTATTNGHPQLPGHAKPGTPALETLETTPPAMAAPKDDGFLNG
SCSGLDEEASGPERPPALLQEEWETVM

Sequence of entity 2 (P), FASTA

>9JR3_2 Parathyroid hormone (chains P)
SVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNFX

Sequence of entity 3 (A), FASTA

>9JR3_3 Guanine nucleotide-binding protein G(i) subunit alpha-1 (miniGq) (chains A)
GSTVSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLGADNSGKSTIVKQMRILHGGSG
GSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEA
LNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPG
EDPRVTRAKYFIRKEFVDISTASGDGRHICYPHFTCAVDTENARRIFNDCKDIILQMNLR
EYNLV

Sequence of entity 4 (B), FASTA

>9JR3_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
GSQLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLA
KIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGG
LDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQ
TTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFP
NGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNV
WDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (G), FASTA

>9JR3_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFS

Sequence of entity 6 (S), FASTA

>9JR3_6 scFv16 (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAAASSEDLYFQ

Primary citation

Insights into G-protein coupling preference from cryo-EM structures of G q -bound PTH1R. Sano, F.K., Shimizume, K., Kobayashi, K. et al. Nat Chem Biol (2025) 21:1906-1914. DOI 10.1038/s41589-025-01957-6 · PubMed

Other PDB entries of the same protein (UniProt Q03431 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5EMB 0.85 Å, Crystal structure of the SNX27 PDZ domain bound to the C-terminal phosphorylated PTHR…
4Z8J 0.95 Å, Crystal structure of the SNX27 PDZ domain bound to the C-terminal PTHR PDZ binding motif
7UZO 1.3 Å, Parathyroid hormone 1 receptor extracellular domain complexed with a peptide ligand…
3H3G 1.94 Å, Crystal structure of the extracellular domain of the human parathyroid hormone receptor…
3C4M 1.95 Å, Structure of human parathyroid hormone in complex with the extracellular domain of its…
8D51 2.0 Å, Parathyroid hormone 1 receptor extracellular domain complexed with a peptide ligand…
8D52 2.02 Å, Parathyroid hormone 1 receptor extracellular domain complexed with a peptide ligand…
7UZP 2.29 Å, parathyroid hormone 1 receptor extracellular domain complexed with a peptide ligand…
8BIA 2.4 Å, Crystal structure of Scribble PDZ1 with PTHR
6FJ3 2.5 Å, High resolution crystal structure of parathyroid hormone 1 receptor in complex with a…
8FLQ 2.55 Å, Human PTH1R in complex with PTH(1-34) and Gs
8JR9 2.57 Å, Small molecule agonist (PCO371) bound to human parathyroid hormone receptor type 1 (PTH1R)

Browse structure collections

About this viewer

MolViewer shows 9JR3 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.