9RHI: Pre-Initiation Complex on ARS1 DNA
pre-Initiation Complex on ARS1 DNA (monomer). Determined by electron microscopy at 3.2 Å resolution. Released 13 May 2026.
- Method
- Electron microscopy
- Resolution
- 3.2 Å
- Organism
- Saccharomyces cerevisiae
- Chains
- 19
- Atoms
- 45,961
- Mol. weight
- 1270.28 kDa
- Ligands
- ZN, ADP, MG
- Released
- 13 May 2026
Explore 9RHI in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
9RHI contains 283 α-helices and 225 β-strands across 17 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain 2: 33 helices, 33 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 184-186 | 3 | |
| α-helix | 194-198 | 5 | |
| α-helix | 201-217 | 17 | |
| α-helix | 226-237 | 12 | |
| β-strand | 241-243 | 3 | 1 |
| α-helix | 246-252 | 7 | |
| α-helix | 254-262 | 9 | |
| α-helix | 264-279 | 16 | |
| α-helix | 286-288 | 3 | |
| β-strand | 294-296 | 3 | 1 |
| β-strand | 304 | 1 | 2 |
| β-strand | 317-327 | 11 | 2 |
| β-strand | 331-337 | 7 | 2 |
| β-strand | 339-341 | 3 | 3 |
| β-strand | 347 | 1 | 3 |
| β-strand | 351-352 | 2 | 2 |
| β-strand | 354 | 1 | 4 |
| β-strand | 357 | 1 | 4 |
| β-strand | 364 | 1 | 5 |
| β-strand | 369 | 1 | 5 |
| β-strand | 373-375 | 3 | 3 |
| β-strand | 381-391 | 11 | 2 |
| α-helix | 398 | 1 | |
| α-helix | 401-403 | 3 | |
| β-strand | 405 | 1 | 2 |
| β-strand | 408-411 | 4 | 2 |
| α-helix | 412-414 | 3 | |
| β-strand | 423-433 | 11 | 2 |
| α-helix | 436-440 | 5 | |
| β-strand | 446 | 1 | 6 |
| β-strand | 448-457 | 10 | 2 |
| α-helix | 478-487 | 10 | |
| α-helix | 493-498 | 6 | |
| α-helix | 509-520 | 12 | |
| β-strand | 525-527 | 3 | 7 |
| β-strand | 531-533 | 3 | 7 |
| β-strand | 539-543 | 5 | 8 |
| α-helix | 550-559 | 10 | |
| β-strand | 563-567 | 5 | 8 |
| α-helix | 568-570 | 3 | |
| α-helix | 573-576 | 4 | |
| β-strand | 578-582 | 5 | 9 |
| β-strand | 589-593 | 5 | 9 |
| α-helix | 595-598 | 4 | |
| β-strand | 602-607 | 6 | 8 |
| α-helix | 614-621 | 8 | |
| α-helix | 622-626 | 5 | |
| β-strand | 628-633 | 6 | 10 |
| β-strand | 636-641 | 6 | 10 |
| β-strand | 644-645 | 2 | 8 |
| β-strand | 647-650 | 4 | 8 |
| α-helix | 653-655 | 3 | |
| α-helix | 663-665 | 3 | |
| α-helix | 671-675 | 5 | |
| β-strand | 679-683 | 5 | 8 |
| α-helix | 689-703 | 15 | |
| α-helix | 740-755 | 16 | |
| α-helix | 760-770 | 11 | |
| β-strand | 777 | 1 | 11 |
| α-helix | 783-795 | 13 | |
| α-helix | 796-798 | 3 | |
| α-helix | 808-823 | 16 | |
| β-strand | 828 | 1 | 11 |
| α-helix | 831-847 | 17 | |
| α-helix | 851-860 | 10 | |
| α-helix | 862-864 | 3 | |
Chain 3: 26 helices, 27 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 21-34 | 14 | |
| α-helix | 38-51 | 14 | |
| β-strand | 95-98 | 4 | 12 |
| α-helix | 100-106 | 7 | |
| α-helix | 108-116 | 9 | |
| α-helix | 118-135 | 18 | |
| β-strand | 154-157 | 4 | 12 |
| α-helix | 173-175 | 3 | |
| β-strand | 178-187 | 10 | 13 |
| β-strand | 193-203 | 11 | 13 |
| β-strand | 209-213 | 5 | 13 |
| β-strand | 234 | 1 | 14 |
| α-helix | 239 | 1 | |
| β-strand | 240 | 1 | 14 |
| β-strand | 241-243 | 3 | 13 |
| β-strand | 249-259 | 11 | 13 |
| α-helix | 262-264 | 3 | |
| α-helix | 265-266 | 2 | |
| α-helix | 270-272 | 3 | |
| β-strand | 273-279 | 7 | 13 |
| α-helix | 280-282 | 3 | |
| β-strand | 291-301 | 11 | 13 |
| β-strand | 318-329 | 12 | 13 |
| α-helix | 344-354 | 11 | |
| α-helix | 359-366 | 8 | |
| α-helix | 376-384 | 9 | |
| β-strand | 391-393 | 3 | 15 |
| β-strand | 397-399 | 3 | 15 |
| β-strand | 405-409 | 5 | 16 |
| α-helix | 415-425 | 11 | |
| β-strand | 429-432 | 4 | 16 |
| β-strand | 437 | 1 | 17 |
| β-strand | 441 | 1 | 17 |
| β-strand | 444-446 | 3 | 18 |
| β-strand | 457-459 | 3 | 18 |
| α-helix | 461-464 | 4 | |
| β-strand | 468-472 | 5 | 16 |
| α-helix | 475-477 | 3 | |
| α-helix | 480-492 | 13 | |
| β-strand | 494-496 | 3 | 19 |
| β-strand | 499 | 1 | 20 |
| β-strand | 502 | 1 | 20 |
| β-strand | 505-507 | 3 | 19 |
| β-strand | 510-516 | 7 | 16 |
| α-helix | 529-532 | 4 | |
| α-helix | 537-540 | 4 | |
| β-strand | 545-549 | 5 | 16 |
| α-helix | 555-569 | 15 | |
| α-helix | 576-577 | 2 | |
| α-helix | 653-663 | 11 | |
| α-helix | 673-687 | 15 | |
| α-helix | 699-715 | 17 | |
| α-helix | 723-738 | 16 | |
Chain 4: 31 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 180 | 1 | 21 |
| α-helix | 187-198 | 12 | |
| α-helix | 203-210 | 8 | |
| α-helix | 213-215 | 3 | |
| α-helix | 225-236 | 12 | |
| β-strand | 240-244 | 5 | 22 |
| α-helix | 252-254 | 3 | |
| α-helix | 255-263 | 9 | |
| α-helix | 265-287 | 23 | |
| α-helix | 293-298 | 6 | |
| β-strand | 302-306 | 5 | 22 |
| α-helix | 319-322 | 4 | |
| β-strand | 325-335 | 11 | 23 |
| β-strand | 339-349 | 11 | 23 |
| β-strand | 355-359 | 5 | 23 |
| β-strand | 361 | 1 | 24 |
| β-strand | 364 | 1 | 24 |
| β-strand | 370 | 1 | 25 |
| α-helix | 374-376 | 3 | |
| β-strand | 379 | 1 | 25 |
| β-strand | 382-384 | 3 | 23 |
| α-helix | 386-388 | 3 | |
| β-strand | 390-401 | 12 | 23 |
| α-helix | 402 | 1 | |
| α-helix | 411-413 | 3 | |
| β-strand | 414-420 | 7 | 23 |
| β-strand | 432-442 | 11 | 23 |
| β-strand | 445 | 1 | 26 |
| β-strand | 453-454 | 2 | 26 |
| β-strand | 456 | 1 | 27 |
| α-helix | 457 | 1 | |
| β-strand | 458-468 | 11 | 23 |
| α-helix | 507-513 | 7 | |
| α-helix | 518-521 | 4 | |
| α-helix | 522-524 | 3 | |
| α-helix | 534-545 | 12 | |
| β-strand | 565-566 | 2 | 28 |
| β-strand | 567-568 | 2 | 29 |
| α-helix | 577-584 | 8 | |
| β-strand | 589-592 | 4 | 28 |
| α-helix | 620-623 | 4 | |
| β-strand | 628-632 | 5 | 28 |
| α-helix | 634-636 | 3 | |
| α-helix | 639-642 | 4 | |
| α-helix | 645-651 | 7 | |
| β-strand | 653-658 | 6 | 30 |
| β-strand | 661-666 | 6 | 30 |
| β-strand | 670-674 | 5 | 28 |
| α-helix | 688-690 | 3 | |
| α-helix | 696-699 | 4 | |
| β-strand | 707-708 | 2 | 29 |
| α-helix | 716-726 | 11 | |
| α-helix | 746-757 | 12 | |
| β-strand | 761 | 1 | 31 |
| α-helix | 766-779 | 14 | |
| α-helix | 795-797 | 3 | |
| α-helix | 798-811 | 14 | |
| β-strand | 816 | 1 | 31 |
| α-helix | 819-834 | 16 | |
Chain 5: 27 helices, 22 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 22-36 | 15 | |
| α-helix | 45-55 | 11 | |
| β-strand | 60-64 | 5 | 32 |
| α-helix | 65-71 | 7 | |
| α-helix | 73-81 | 9 | |
| α-helix | 83-102 | 20 | |
| α-helix | 134-135 | 2 | |
| β-strand | 136-140 | 5 | 32 |
| α-helix | 143-147 | 5 | |
| α-helix | 148-150 | 3 | |
| α-helix | 153-155 | 3 | |
| β-strand | 158-168 | 11 | 33 |
| β-strand | 173-183 | 11 | 33 |
| β-strand | 190-193 | 4 | 33 |
| β-strand | 241-259 | 19 | 33 |
| α-helix | 260-261 | 2 | |
| α-helix | 262-264 | 3 | |
| β-strand | 273-279 | 7 | 33 |
| α-helix | 280-282 | 3 | |
| β-strand | 291-299 | 9 | 33 |
| β-strand | 327-335 | 9 | 33 |
| α-helix | 351-361 | 11 | |
| α-helix | 366-373 | 8 | |
| α-helix | 382-393 | 12 | |
| β-strand | 398-399 | 2 | 34 |
| β-strand | 405-406 | 2 | 34 |
| β-strand | 412-415 | 4 | 35 |
| α-helix | 422-432 | 11 | |
| β-strand | 436-440 | 5 | 35 |
| α-helix | 446-449 | 4 | |
| β-strand | 451-456 | 6 | 36 |
| β-strand | 461-466 | 6 | 36 |
| α-helix | 468-471 | 4 | |
| β-strand | 476-480 | 5 | 35 |
| α-helix | 482-484 | 3 | |
| α-helix | 487-499 | 13 | |
| β-strand | 501-504 | 4 | 37 |
| β-strand | 511-514 | 4 | 37 |
| β-strand | 518-523 | 6 | 35 |
| α-helix | 536-539 | 4 | |
| α-helix | 544-549 | 6 | |
| β-strand | 552-554 | 3 | 35 |
| α-helix | 562-577 | 16 | |
| α-helix | 596-609 | 14 | |
| β-strand | 613-614 | 2 | 38 |
| α-helix | 616-634 | 19 | |
| α-helix | 650-666 | 17 | |
| β-strand | 671-672 | 2 | 38 |
| α-helix | 674-693 | 20 | |
Chain 6: 28 helices, 35 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 108-121 | 14 | |
| β-strand | 124-126 | 3 | 39 |
| β-strand | 131-133 | 3 | 39 |
| α-helix | 139-145 | 7 | |
| β-strand | 150-154 | 5 | 40 |
| α-helix | 162-164 | 3 | |
| α-helix | 165-172 | 8 | |
| α-helix | 178-192 | 15 | |
| α-helix | 194-197 | 4 | |
| β-strand | 198 | 1 | 41 |
| β-strand | 260 | 1 | 41 |
| α-helix | 261-262 | 2 | |
| β-strand | 264-268 | 5 | 40 |
| β-strand | 287 | 1 | 6 |
| β-strand | 290-296 | 7 | 6 |
| β-strand | 301-311 | 11 | 6 |
| β-strand | 317-322 | 6 | 6 |
| β-strand | 332 | 1 | 42 |
| β-strand | 341 | 1 | 42 |
| β-strand | 344-346 | 3 | 6 |
| β-strand | 352-362 | 11 | 6 |
| α-helix | 372-375 | 4 | |
| β-strand | 376-382 | 7 | 6 |
| α-helix | 383-385 | 3 | |
| β-strand | 394-405 | 12 | 6 |
| β-strand | 437 | 1 | 43 |
| β-strand | 438 | 1 | 24 |
| β-strand | 448 | 1 | 43 |
| β-strand | 450-461 | 12 | 6 |
| α-helix | 501-504 | 4 | |
| α-helix | 511-521 | 11 | |
| α-helix | 525-532 | 8 | |
| α-helix | 542-550 | 9 | |
| α-helix | 553-556 | 4 | |
| β-strand | 557-558 | 2 | 44 |
| β-strand | 564-565 | 2 | 44 |
| β-strand | 571-572 | 2 | 45 |
| β-strand | 574-575 | 2 | 46 |
| α-helix | 582-589 | 8 | |
| β-strand | 597-599 | 3 | 45 |
| α-helix | 605-608 | 4 | |
| β-strand | 611-613 | 3 | 47 |
| β-strand | 622-624 | 3 | 47 |
| α-helix | 627-630 | 4 | |
| β-strand | 635-639 | 5 | 45 |
| α-helix | 641-643 | 3 | |
| α-helix | 646-651 | 6 | |
| α-helix | 653-658 | 6 | |
| β-strand | 660-665 | 6 | 48 |
| β-strand | 668-673 | 6 | 48 |
| β-strand | 677-680 | 4 | 45 |
| α-helix | 695-698 | 4 | |
| α-helix | 703-708 | 6 | |
| β-strand | 711 | 1 | 45 |
| β-strand | 714-715 | 2 | 46 |
| α-helix | 721-727 | 7 | |
| α-helix | 729-734 | 6 | |
| α-helix | 749-758 | 10 | |
| β-strand | 764 | 1 | 49 |
| α-helix | 767-782 | 16 | |
| α-helix | 797-814 | 18 | |
| β-strand | 818 | 1 | 49 |
| α-helix | 821-835 | 15 | |
Chain 7: 26 helices, 27 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 8 | 1 | 13 |
| α-helix | 14-24 | 11 | |
| α-helix | 62-71 | 10 | |
| β-strand | 77-81 | 5 | 50 |
| α-helix | 82-95 | 14 | |
| α-helix | 102-108 | 7 | |
| α-helix | 113-123 | 11 | |
| α-helix | 126-128 | 3 | |
| α-helix | 139-154 | 16 | |
| β-strand | 201-205 | 5 | 50 |
| α-helix | 206-209 | 4 | |
| α-helix | 220-222 | 3 | |
| β-strand | 225 | 1 | 27 |
| α-helix | 232-234 | 3 | |
| β-strand | 237-248 | 12 | 27 |
| β-strand | 252-262 | 11 | 27 |
| β-strand | 268-272 | 5 | 27 |
| β-strand | 277-278 | 2 | 26 |
| β-strand | 283 | 1 | 51 |
| α-helix | 287-291 | 5 | |
| β-strand | 297 | 1 | 51 |
| β-strand | 298-300 | 3 | 27 |
| β-strand | 306-316 | 11 | 27 |
| α-helix | 323 | 1 | |
| β-strand | 330-336 | 7 | 27 |
| β-strand | 348-358 | 11 | 27 |
| β-strand | 373-383 | 11 | 27 |
| α-helix | 397-407 | 11 | |
| α-helix | 410-414 | 5 | |
| α-helix | 426-437 | 12 | |
| β-strand | 456-460 | 5 | 52 |
| α-helix | 466-476 | 11 | |
| β-strand | 481-484 | 4 | 53 |
| α-helix | 512-515 | 4 | |
| β-strand | 519-524 | 6 | 53 |
| α-helix | 526-528 | 3 | |
| α-helix | 531-542 | 12 | |
| β-strand | 545-547 | 3 | 54 |
| β-strand | 550 | 1 | 55 |
| β-strand | 553 | 1 | 55 |
| β-strand | 556-558 | 3 | 54 |
| β-strand | 561-563 | 3 | 53 |
| β-strand | 564-566 | 3 | 52 |
| α-helix | 580-583 | 4 | |
| α-helix | 588-593 | 6 | |
| β-strand | 596-600 | 5 | 52 |
| α-helix | 606-622 | 17 | |
| α-helix | 635-646 | 12 | |
| β-strand | 651 | 1 | 56 |
| α-helix | 654-670 | 17 | |
| α-helix | 686-702 | 17 | |
| β-strand | 707 | 1 | 56 |
| α-helix | 710-725 | 16 | |
Chain C: 10 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-11 | 6 | |
| β-strand | 15-20 | 6 | 57 |
| β-strand | 24 | 1 | 58 |
| α-helix | 28-31 | 4 | |
| β-strand | 38 | 1 | 58 |
| β-strand | 43-47 | 5 | 57 |
| α-helix | 48-56 | 9 | |
| β-strand | 58 | 1 | 59 |
| β-strand | 70 | 1 | 59 |
| β-strand | 72-74 | 3 | 57 |
| α-helix | 78-80 | 3 | |
| α-helix | 82-90 | 9 | |
| α-helix | 97-100 | 4 | |
| α-helix | 104-115 | 12 | |
| α-helix | 118-140 | 23 | |
| α-helix | 160-166 | 7 | |
| α-helix | 171-192 | 22 | |
Chain D: 9 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 56-72 | 17 | |
| α-helix | 81-103 | 23 | |
| α-helix | 120-153 | 34 | |
| α-helix | 155-163 | 9 | |
| α-helix | 171-174 | 4 | |
| α-helix | 177-195 | 19 | |
| α-helix | 199-201 | 3 | |
| α-helix | 204-206 | 3 | |
| β-strand | 227-232 | 6 | 60 |
| β-strand | 247-248 | 2 | 61 |
| β-strand | 254-260 | 7 | 61 |
| β-strand | 265-270 | 6 | 61 |
| β-strand | 274-278 | 5 | 60 |
| α-helix | 279-287 | 9 | |
| β-strand | 291-293 | 3 | 60 |
9 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| DNA replication licensing factor MCM2 | 2 | protein | 868 | Saccharomyces cerevisiae | P29469 (AlphaFold model) |
| DNA replication licensing factor MCM3 | 3 | protein | 1006 | Saccharomyces cerevisiae | P24279 (AlphaFold model) |
| DNA replication licensing factor MCM4 | 4 | protein | 933 | Saccharomyces cerevisiae | P30665 (AlphaFold model) |
| Minichromosome maintenance protein 5 | 5 | protein | 775 | Saccharomyces cerevisiae | P29496 (AlphaFold model) |
| DNA replication licensing factor MCM6 | 6 | protein | 1017 | Saccharomyces cerevisiae | P53091 |
| DNA replication licensing factor MCM7 | 7, f | protein | 845 | Saccharomyces cerevisiae | P38132 |
| DNA (31-mer) | A | DNA | 31 | Saccharomyces cerevisiae | |
| DNA (31-mer) | B | DNA | 31 | Saccharomyces cerevisiae | |
| DNA replication complex GINS protein PSF3 | C | protein | 217 | Saccharomyces cerevisiae | Q12146 |
| DNA replication complex GINS protein SLD5 | D | protein | 294 | Saccharomyces cerevisiae | Q03406 |
| Cell division control protein 45 | E | protein | 657 | Saccharomyces cerevisiae | Q08032 |
| DNA polymerase epsilon subunit B | F | protein | 689 | Saccharomyces cerevisiae | P24482 |
5 more molecules are not listed.
Sequence of entity 1 (2), FASTA
>9RHI_1 DNA replication licensing factor MCM2 (chains 2)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH
Sequence of entity 2 (3), FASTA
>9RHI_2 DNA replication licensing factor MCM3 (chains 3)
MKRRWKKNFIAVSAANRFKKISSSGALENLYFQGEMEGSTGFDGDATTFFAPDAVFGDRV
RRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQDDADERDLLGDDDGDDLEKEKKAAS
STSLNILPHRIIISLDDLREFDRSFWSGILVEPAYFIPPAEKALTDLADSMDDVPHPNAS
AVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLVSVEGIVTKTSLVRPKLIRSVHYAAK
TGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNKLTTEYGYSTFIDHQRITVQEMPEMA
PAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKSLGAGGMNQSNSNTLIGFKTLILGNT
VYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIFDILSQSLAPSIYGHDHIKKAILLML
MGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLRFVLNTASLAIATTGRGSSGVGLTAA
VTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTDVDRVAIHEVMEQQTVTIAKAGIHTT
LNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLLSRFDLLFVVTDDINEIRDRSISEHV
LRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINPEEHSNSGAGVENEGEDDEDHVFEKF
NPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYVQYAKERVIPQLTQEAINVIVKNYTD
LRNDDNTKKSPITARTLETLIRLATAHAKVRLSKTVNKVDAKVAANLLRFALLGEDIGND
IDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSGSPIKSTPRRSTASSVNATPSSARRI
LRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQLGLRVSPRRREHLHAPEEGSSGPLTE
VGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTISTGRLSLISGIIARLMQTEIFEEESYP
VASLFERINEELPEEEKFSAQEYLAGLKIMSDRNNLMVADDKVWRV
Sequence of entity 3 (4), FASTA
>9RHI_3 DNA replication licensing factor MCM4 (chains 4)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV
Sequence of entity 4 (5), FASTA
>9RHI_4 Minichromosome maintenance protein 5 (chains 5)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV
Sequence of entity 5 (6), FASTA
>9RHI_5 DNA replication licensing factor MCM6 (chains 6)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS
Sequence of entity 6 (7, f), FASTA
>9RHI_6 DNA replication licensing factor MCM7 (chains 7, f)
MSAALPSIQLPVDYNNLFNEITDFLVTFKQDTLSSDATRNENEDENLDAENIEQHLLEKG
PKYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRA
IDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDA
LREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLIT
VRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFM
STRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPY
TGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAP
EIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRG
VYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEV
MEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLM
LDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYV
VQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLV
RVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLS
NCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANVSAQDSDI
DLQDA
Sequence of entity 7 (A), FASTA
>9RHI_7 DNA (31-MER) (chains A)
AGTCAGCTACCATATAGAGATACATACATAT
Sequence of entity 8 (B), FASTA
>9RHI_8 DNA (31-MER) (chains B)
ATATGTATGTATCTCTATATGGTAGCTGACT
Sequence of entity 9 (C), FASTA
>9RHI_9 DNA replication complex GINS protein PSF3 (chains C)
MGSSHHHHHHSSGLVPRGSHMASMGYYDIDDVLADGTEFPCKFQYDIPGLGYLENNPGRP
ITKNTKLSLPLWLARILAIVGGDEALVDEEPVPFVELLPPDMFSTKVMNAIKTDPVALDL
HSINSHFFSLAIKWIMLFSEKELANVVSELLLQRAQELNHHASSLSIDLNADSTGKNSAN
TNIATSTFLLKLEEMEKEIYKKSHESYKDTKRWMFKK
Sequence of entity 10 (D), FASTA
>9RHI_10 DNA replication complex GINS protein SLD5 (chains D)
MDINIDDILAELDKETTAVDSTKITQGSSSTTHRDANTIVGSSLDLNDKTQIYVSPQQDF
SDLMKSWKNERCSPELLPYPHQLMKRLLNRISMQSQLIENISMGFLDMQNASNANPPMPN
ESKLPLLCMETELERLKFVIRSYIRCRLSKIDKFSLYLRQLNEDENSLISLTDLLSKDEI
KYHDTHSLIWLKLVNDSILKYMPEELQAINDTEGSVNMIDEPDWNKFVFIHVNGPPDGKW
NEDPLLQENEFGKPCYTVTIPDLKEEVELTIGSIYVMRYEVIRDLLRDDKVALI
Sequence of entity 11 (E), FASTA
>9RHI_11 Cell division control protein 45 (chains E)
MYYGISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFKKQLVQSQIVP
IFGYSELRRHYSQLDDNINSLLLVGFGGVIDLEAFLEIDPQEYVIDTDEKSGEQSFRRDI
YVLDAHRPWNLDNIFGSQIIQCFDDGTVDDTLGEQKEAYYKLLELDEESGDDELSGDEND
NNGGDDEATDADEVTDEDYKDDDGDYKDDDETISNKRGNSSIGPNDLSKRKQRKKQIHEY
EGVLEEYYSQGTTVVNSISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYP
LLQDEVKRLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSLWNE
NGKKRLHKMFARMGIPLSTAQETWLYMDHSIKRELGIIFDKNLDRYGLQDIIRDGFVRTL
GYRGSISASEFVEALTALLEVGNSTDKDSVKINNDNNDDTDGEEEEDNSAQKLTNLRKRW
VSNFWLSWDALDDRKVELLNRGIQLAQDLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDG
PDLDLYRNPLTLLRLGNWLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGLD
TIHTKKPILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL
Sequence of entity 12 (F), FASTA
>9RHI_12 DNA polymerase epsilon subunit B (chains F)
MFGSGNVLPVKIQPPLLRPLAYRVLSRKYGLSIKSDGLSALAEFVGTNIGANWRQGPATI
KFLEQFAAVWKQQERGLFIDQSGVKEVIQEMKEREKVEWSHEHPIQHEENILGRTDDDEN
NSDDEMPIAADSSLQNVSLSSPMRQPTERDEYKQPFKPESSKALDWRDYFKVINASQQQR
FSYNPHKMQFIFVPNKKQNGLGGIAGFLPDIEDKVQMFLTRYYLTNDRVMRNENFQNSDM
FNPLSSMVSLQNELSNTNRQQQSSSMSITPIKNLLGRDAQNFLLLGLLNKNFKGNWSLED
PSGSVEIDISQTIPTQGHYYVPGCMVLVEGIYYSVGNKFHVTSMTLPPGERREITLETIG
NLDLLGIHGISNNNFIARLDKDLKIRLHLLEKELTDHKFVILGANLFLDDLKIMTALSKI
LQKLNDDPPTLLIWQGSFTSVPVFASMSSRNISSSTQFKNNFDALATLLSRFDNLTENTT
MIFIPGPNDLWGSMVSLGASGTLPQDPIPSAFTKKINKVCKNVVWSSNPTRIAYLSQEIV
IFRDDLSGRFKRHRLEFPFNESEDVYTENDNMMSKDTDIVPIDELVKEPDQLPQKVQETR
KLVKTILDQGHLSPFLDSLRPISWDLDHTLTLCPIPSTMVLCDTTSAQFDLTYNGCKVIN
PGSFIHNRRARYMEYVPSSKKTIQEEIYI
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ZN | Zinc ion | Zn | 5 |
| ADP | Adenosine-5'-diphosphate | C10 H15 N5 O10 P2 | 1 |
| MG | Magnesium ion | Mg | 1 |
Primary citation
Structure of the pre-initiation complex explains CMGE biogenesis. Puhringer, T., Canal, B., Palm, G. et al. Nature (2026) 655:1330-1338. DOI 10.1038/s41586-026-10657-7 · PubMed
Other PDB entries of the same protein (UniProt P29469 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7W8G 2.52 Å, Cryo-EM structure of MCM double hexamer
- 28VY 2.7 Å, sCMGE assembled on ARS1 DNA with Sld2 and RPA
- 8RIF 2.79 Å, Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
- 7V3V 2.9 Å, Cryo-EM structure of MCM double hexamer bound with DDK in State I
- 9TH8 2.9 Å, Cryo-EM structure of MCM2-7 DH bound to Sld3-Sld7, Cdc45 and DNA
- 7P30 3.0 Å, 3.0 A resolution structure of a DNA-loaded MCM double hexamer
- 8KG6 3.07 Å, Yeast replisome in state I
- 9RHL 3.1 Å, Phospho-DH bound by Sld3-MBD on ARS1 DNA
- 7PMK 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
- 7PMN 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
- 7PT6 3.2 Å, Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III
- 7V3U 3.2 Å, Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD
Browse structure collections
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