9RHJ: Pre-Initiation Complex on ARS1 DNA

Pre-Initiation Complex on ARS1 DNA (dimer). Determined by electron microscopy at 3.4 Å resolution. Released 13 May 2026.

Method: Electron microscopy
Resolution: 3.4 Å
Organisms: Saccharomyces cerevisiae, synthetic construct
Chains: 32
Atoms: 91,686
Mol. weight: 2186.84 kDa
Ligands: MG, ADP, ZN
Released: 13 May 2026

Explore 9RHJ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9RHJ contains 569 α-helices and 441 β-strands across 30 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 2: 32 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	184-186	3
α-helix	194-199	6
α-helix	201-217	17
α-helix	226-237	12
β-strand	241-243	3	1
α-helix	246-252	7
α-helix	254-262	9
α-helix	264-279	16
α-helix	286-288	3
β-strand	294-296	3	1
β-strand	317-327	11	2
β-strand	331-337	7	2
β-strand	339-341	3	3
α-helix	349-350	2
β-strand	351-352	2	2
β-strand	354	1	4
β-strand	357	1	4
β-strand	364	1	5
β-strand	369	1	5
β-strand	373-375	3	3
β-strand	381-391	11	2
α-helix	398	1
α-helix	401-403	3
β-strand	405	1	2
β-strand	408-411	4	2
α-helix	412-414	3
β-strand	423-433	11	2
α-helix	436-442	7
β-strand	446	1	6
β-strand	448-457	10	2
α-helix	478-487	10
α-helix	493-498	6
α-helix	509-520	12
β-strand	525-527	3	7
β-strand	531-533	3	7
β-strand	539-543	5	8
α-helix	550-559	10
β-strand	563-567	5	8
α-helix	568-570	3
α-helix	573-576	4
β-strand	578-582	5	9
β-strand	589-593	5	9
α-helix	595-598	4
β-strand	602-607	6	8
α-helix	614-624	11
β-strand	628-633	6	10
β-strand	636-641	6	10
β-strand	644-645	2	8
β-strand	647-650	4	8
α-helix	653-655	3
α-helix	663-665	3
α-helix	671-675	5
β-strand	679-683	5	8
α-helix	689-706	18
α-helix	740-755	16
α-helix	760-770	11
β-strand	777	1	11
α-helix	783-795	13
α-helix	807-823	17
β-strand	828	1	11
α-helix	831-847	17
α-helix	851-860	10
α-helix	863-866	4

Chain 3: 28 helices, 27 β-strands

Element	Residues	Length	Sheet
α-helix	21-34	14
α-helix	41-51	11
β-strand	95-98	4	23
α-helix	100-106	7
α-helix	108-116	9
α-helix	118-135	18
β-strand	154-157	4	23
α-helix	173-175	3
β-strand	178-187	10	24
β-strand	193-203	11	24
β-strand	209-213	5	24
β-strand	234	1	25
α-helix	239	1
β-strand	240	1	25
β-strand	241-243	3	24
β-strand	249-259	11	24
α-helix	262-264	3
α-helix	265-266	2
α-helix	270-272	3
β-strand	273-279	7	24
α-helix	280-282	3
β-strand	291-301	11	24
β-strand	318-329	12	24
α-helix	344-354	11
α-helix	359-366	8
α-helix	376-385	10
α-helix	387-390	4
β-strand	391-393	3	26
β-strand	397-399	3	26
β-strand	405-409	5	27
α-helix	415-425	11
β-strand	429-432	4	27
β-strand	437	1	28
β-strand	441	1	28
β-strand	444-446	3	29
β-strand	457-459	3	29
α-helix	460	1
α-helix	461-464	4
β-strand	469-472	4	27
α-helix	475-477	3
α-helix	480-492	13
β-strand	494-496	3	30
β-strand	499	1	31
β-strand	502	1	31
β-strand	505-507	3	30
β-strand	511-516	6	27
α-helix	529-532	4
α-helix	537-540	4
β-strand	545-549	5	27
α-helix	555-569	15
α-helix	576-577	2
α-helix	653-663	11
α-helix	673-687	15
α-helix	700-715	16
α-helix	723-738	16

Chain 4: 31 helices, 28 β-strands

Element	Residues	Length	Sheet
β-strand	180	1	41
α-helix	187-198	12
α-helix	203-210	8
α-helix	213-215	3
α-helix	225-236	12
β-strand	240-244	5	42
α-helix	252-254	3
α-helix	256-263	8
α-helix	265-287	23
α-helix	293-298	6
β-strand	302-306	5	42
α-helix	320-322	3
β-strand	325-335	11	43
β-strand	339-349	11	43
β-strand	355-359	5	43
β-strand	361	1	44
β-strand	364	1	44
β-strand	370	1	45
α-helix	374-376	3
β-strand	379	1	45
β-strand	382-384	3	43
α-helix	386-388	3
β-strand	390-401	12	43
α-helix	402	1
β-strand	414-420	7	43
α-helix	421-423	3
β-strand	432-442	11	43
β-strand	445	1	46
β-strand	453-454	2	46
β-strand	456	1	47
α-helix	457	1
β-strand	458-468	11	43
α-helix	506-513	8
α-helix	518-521	4
α-helix	522-524	3
α-helix	534-545	12
β-strand	565-566	2	48
β-strand	567-568	2	49
α-helix	577-583	7
β-strand	589-592	4	48
α-helix	620-623	4
β-strand	628-632	5	48
α-helix	634-636	3
α-helix	639-642	4
α-helix	645-651	7
β-strand	653-658	6	50
β-strand	661-666	6	50
β-strand	670-674	5	48
α-helix	688-690	3
α-helix	696-699	4
β-strand	707-708	2	49
α-helix	719-725	7
α-helix	726-728	3
α-helix	746-757	12
β-strand	761	1	51
α-helix	766-779	14
α-helix	798-811	14
β-strand	816	1	51
α-helix	819-834	16

Chain 5: 27 helices, 22 β-strands

Element	Residues	Length	Sheet
α-helix	22-36	15
α-helix	45-55	11
β-strand	60-64	5	63
α-helix	65-71	7
α-helix	73-81	9
α-helix	83-102	20
α-helix	134-135	2
β-strand	136-140	5	63
α-helix	144-147	4
α-helix	148-150	3
α-helix	153-155	3
β-strand	158-168	11	64
β-strand	173-183	11	64
β-strand	189-193	5	64
β-strand	241-259	19	64
α-helix	260-261	2
α-helix	262-264	3
β-strand	273-279	7	64
α-helix	280-282	3
β-strand	291-299	9	64
β-strand	327-335	9	64
α-helix	351-361	11
α-helix	366-373	8
α-helix	382-393	12
β-strand	398-399	2	65
β-strand	405-406	2	65
β-strand	412-415	4	66
α-helix	422-432	11
β-strand	436-440	5	66
α-helix	446-449	4
β-strand	451-456	6	67
β-strand	461-466	6	67
α-helix	468-471	4
β-strand	476-480	5	66
α-helix	482-484	3
α-helix	487-499	13
β-strand	501-504	4	68
β-strand	511-514	4	68
β-strand	518-523	6	66
α-helix	536-539	4
α-helix	544-549	6
β-strand	552-554	3	66
α-helix	562-577	16
α-helix	596-609	14
β-strand	613	1	69
α-helix	616-634	19
α-helix	650-666	17
β-strand	671	1	69
α-helix	674-693	20

Chain 6: 27 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	108-121	14
β-strand	124-126	3	77
β-strand	131-133	3	77
α-helix	139-145	7
β-strand	150-154	5	78
α-helix	162-164	3
α-helix	165-172	8
α-helix	178-192	15
α-helix	194-197	4
β-strand	198	1	79
β-strand	260	1	79
α-helix	261-262	2
β-strand	264-268	5	78
β-strand	287	1	6
β-strand	290-296	7	6
β-strand	301-311	11	6
β-strand	317-322	6	6
β-strand	344-346	3	6
β-strand	352-362	11	6
α-helix	372-375	4
β-strand	376-382	7	6
α-helix	383-385	3
β-strand	394-404	11	6
β-strand	438	1	44
β-strand	451-461	11	6
α-helix	501-504	4
α-helix	511-521	11
α-helix	525-532	8
α-helix	542-550	9
α-helix	553-556	4
β-strand	557-558	2	80
β-strand	564-565	2	80
β-strand	571-572	2	81
β-strand	574-575	2	82
α-helix	582-589	8
β-strand	597-599	3	81
α-helix	605-608	4
β-strand	611-613	3	83
β-strand	622-624	3	83
α-helix	627-630	4
β-strand	635-639	5	81
α-helix	641-643	3
α-helix	646-651	6
α-helix	653-658	6
β-strand	660-665	6	84
β-strand	668-673	6	84
β-strand	677-680	4	81
α-helix	695-698	4
α-helix	703-708	6
β-strand	711	1	81
β-strand	714-715	2	82
α-helix	721-733	13
α-helix	749-759	11
β-strand	764	1	85
α-helix	767-782	16
α-helix	797-814	18
β-strand	818	1	85
α-helix	821-835	15

Chain 7: 29 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	8	1	24
α-helix	14-24	11
α-helix	62-71	10
β-strand	77-81	5	97
α-helix	82-95	14
α-helix	102-108	7
α-helix	111-123	13
α-helix	126-128	3
α-helix	139-142	4
α-helix	144-154	11
α-helix	157-164	8
α-helix	172-174	3
α-helix	175-188	14
β-strand	201-205	5	97
α-helix	206-209	4
α-helix	220-222	3
β-strand	225	1	47
α-helix	232-234	3
β-strand	237-262	26	47
β-strand	268-272	5	47
β-strand	277-278	2	46
β-strand	283	1	98
α-helix	287-291	5
β-strand	297	1	98
β-strand	298-300	3	47
β-strand	306-316	11	47
β-strand	330-336	7	47
β-strand	347-358	12	47
β-strand	371	1	24
β-strand	373-383	11	47
α-helix	397-407	11
α-helix	410-414	5
α-helix	426-437	12
β-strand	456-460	5	99
α-helix	466-476	11
β-strand	481-484	4	100
α-helix	512-515	4
β-strand	519-524	6	100
α-helix	526-528	3
α-helix	531-543	13
β-strand	545-547	3	101
β-strand	550	1	102
β-strand	553	1	102
β-strand	556-558	3	101
β-strand	561-563	3	100
β-strand	564-566	3	99
α-helix	580-583	4
α-helix	588-593	6
β-strand	596-600	5	99
α-helix	606-622	17
α-helix	635-646	12
β-strand	651	1	103
α-helix	655-670	16
α-helix	686-702	17
β-strand	707	1	103
α-helix	710-725	16

Chain a: 35 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	184-186	3
α-helix	194-198	5
α-helix	201-217	17
α-helix	226-237	12
β-strand	241-243	3	12
α-helix	246-252	7
α-helix	254-262	9
α-helix	264-279	16
α-helix	286-288	3
β-strand	294-296	3	12
β-strand	304	1	13
β-strand	317-327	11	13
β-strand	331-337	7	13
β-strand	339-341	3	14
α-helix	349-350	2
β-strand	351-352	2	13
β-strand	354	1	15
β-strand	357	1	15
β-strand	363-364	2	16
β-strand	369-370	2	16
β-strand	373-375	3	14
β-strand	381-391	11	13
α-helix	398	1
α-helix	401-403	3
β-strand	405	1	13
β-strand	408-411	4	13
α-helix	412-414	3
β-strand	423-433	11	13
α-helix	436-442	7
β-strand	446	1	17
β-strand	448-457	10	13
α-helix	478-487	10
α-helix	493-498	6
α-helix	509-520	12
β-strand	525-527	3	18
β-strand	531-533	3	18
β-strand	539-543	5	19
α-helix	550-559	10
β-strand	563-567	5	19
α-helix	568-570	3
α-helix	573-576	4
β-strand	578-582	5	20
β-strand	589-593	5	20
α-helix	595-598	4
β-strand	602-607	6	19
α-helix	615-618	4
α-helix	619-621	3
α-helix	622-626	5
β-strand	628-633	6	21
β-strand	636-641	6	21
β-strand	644-650	7	19
α-helix	653-655	3
α-helix	663-665	3
α-helix	671-675	5
β-strand	679-683	5	19
α-helix	689-706	18
α-helix	740-755	16
α-helix	760-770	11
β-strand	777	1	22
α-helix	783-795	13
α-helix	796-798	3
α-helix	807-823	17
β-strand	828	1	22
α-helix	831-848	18
α-helix	851-860	10
α-helix	863-866	4

Chain b: 28 helices, 27 β-strands

Element	Residues	Length	Sheet
α-helix	21-34	14
α-helix	38-51	14
β-strand	95-98	4	32
α-helix	100-104	5
α-helix	108-116	9
α-helix	118-135	18
β-strand	154-157	4	32
α-helix	173-175	3
β-strand	178-187	10	33
β-strand	193-203	11	33
β-strand	209-213	5	33
β-strand	234	1	34
α-helix	239	1
β-strand	240	1	34
β-strand	241-243	3	33
β-strand	249-259	11	33
α-helix	262-264	3
α-helix	265-266	2
α-helix	270-272	3
β-strand	273-279	7	33
α-helix	280-282	3
β-strand	291-301	11	33
β-strand	318-329	12	33
α-helix	344-354	11
α-helix	359-366	8
α-helix	376-385	10
α-helix	387-390	4
β-strand	391-393	3	35
β-strand	397-399	3	35
β-strand	405-409	5	36
α-helix	415-425	11
β-strand	429-432	4	36
β-strand	437	1	37
β-strand	441	1	37
β-strand	444-446	3	38
β-strand	457-459	3	38
α-helix	460	1
α-helix	461-464	4
β-strand	469-472	4	36
α-helix	475-477	3
α-helix	480-492	13
β-strand	494-496	3	39
β-strand	499	1	40
β-strand	502	1	40
β-strand	505-507	3	39
β-strand	511-516	6	36
α-helix	529-532	4
α-helix	537-541	5
β-strand	545-549	5	36
α-helix	555-569	15
α-helix	576-577	2
α-helix	653-663	11
α-helix	673-687	15
α-helix	700-716	17
α-helix	723-738	16

22 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA replication licensing factor MCM2	2, a	protein	868	Saccharomyces cerevisiae	P29469 (AlphaFold model)
DNA replication licensing factor MCM3	3, b	protein	1006	Saccharomyces cerevisiae	P24279 (AlphaFold model)
DNA replication licensing factor MCM4	4, c	protein	933	Saccharomyces cerevisiae	P30665 (AlphaFold model)
Minichromosome maintenance protein 5	5, d	protein	775	Saccharomyces cerevisiae	P29496 (AlphaFold model)
DNA replication licensing factor MCM6	6, e	protein	1017	Saccharomyces cerevisiae	P53091
DNA replication licensing factor MCM7	7, f	protein	845	Saccharomyces cerevisiae	P38132
DNA (60-mer)	A	DNA	60	synthetic construct
DNA (60-mer)	B	DNA	60	synthetic construct
DNA replication complex GINS protein PSF3	C, J	protein	217	Saccharomyces cerevisiae	Q12146
DNA replication complex GINS protein SLD5	D, K	protein	294	Saccharomyces cerevisiae	Q03406
Cell division control protein 45	E, L	protein	657	Saccharomyces cerevisiae	Q08032
DNA polymerase epsilon subunit B	F, M	protein	689	Saccharomyces cerevisiae	P24482

5 more molecules are not listed.

Sequence of entity 1 (2, a), FASTA

>9RHJ_1 DNA replication licensing factor MCM2 (chains 2, a)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH

Sequence of entity 2 (3, b), FASTA

>9RHJ_2 DNA replication licensing factor MCM3 (chains 3, b)
MKRRWKKNFIAVSAANRFKKISSSGALENLYFQGEMEGSTGFDGDATTFFAPDAVFGDRV
RRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQDDADERDLLGDDDGDDLEKEKKAAS
STSLNILPHRIIISLDDLREFDRSFWSGILVEPAYFIPPAEKALTDLADSMDDVPHPNAS
AVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLVSVEGIVTKTSLVRPKLIRSVHYAAK
TGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNKLTTEYGYSTFIDHQRITVQEMPEMA
PAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKSLGAGGMNQSNSNTLIGFKTLILGNT
VYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIFDILSQSLAPSIYGHDHIKKAILLML
MGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLRFVLNTASLAIATTGRGSSGVGLTAA
VTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTDVDRVAIHEVMEQQTVTIAKAGIHTT
LNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLLSRFDLLFVVTDDINEIRDRSISEHV
LRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINPEEHSNSGAGVENEGEDDEDHVFEKF
NPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYVQYAKERVIPQLTQEAINVIVKNYTD
LRNDDNTKKSPITARTLETLIRLATAHAKVRLSKTVNKVDAKVAANLLRFALLGEDIGND
IDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSGSPIKSTPRRSTASSVNATPSSARRI
LRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQLGLRVSPRRREHLHAPEEGSSGPLTE
VGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTISTGRLSLISGIIARLMQTEIFEEESYP
VASLFERINEELPEEEKFSAQEYLAGLKIMSDRNNLMVADDKVWRV

Sequence of entity 3 (4, c), FASTA

>9RHJ_3 DNA replication licensing factor MCM4 (chains 4, c)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV

Sequence of entity 4 (5, d), FASTA

>9RHJ_4 Minichromosome maintenance protein 5 (chains 5, d)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV

Sequence of entity 5 (6, e), FASTA

>9RHJ_5 DNA replication licensing factor MCM6 (chains 6, e)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS

Sequence of entity 6 (7, f), FASTA

>9RHJ_6 DNA replication licensing factor MCM7 (chains 7, f)
MSAALPSIQLPVDYNNLFNEITDFLVTFKQDTLSSDATRNENEDENLDAENIEQHLLEKG
PKYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRA
IDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDA
LREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLIT
VRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFM
STRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPY
TGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAP
EIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRG
VYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEV
MEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLM
LDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYV
VQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLV
RVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLS
NCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANVSAQDSDI
DLQDA

Sequence of entity 7 (A), FASTA

>9RHJ_7 DNA (60-MER) (chains A)
ATATGTATGTATCTCTATATGGTAGCTGAAGTCAGCTACCATATAGAGATACATACATAT

Sequence of entity 8 (B), FASTA

>9RHJ_8 DNA (60-MER) (chains B)
ATATGTATGTATCTCTATATGGTAGCTGACTTCAGCTACCATATAGAGATACATACATAT

Sequence of entity 9 (C, J), FASTA

>9RHJ_9 DNA replication complex GINS protein PSF3 (chains C, J)
MGSSHHHHHHSSGLVPRGSHMASMGYYDIDDVLADGTEFPCKFQYDIPGLGYLENNPGRP
ITKNTKLSLPLWLARILAIVGGDEALVDEEPVPFVELLPPDMFSTKVMNAIKTDPVALDL
HSINSHFFSLAIKWIMLFSEKELANVVSELLLQRAQELNHHASSLSIDLNADSTGKNSAN
TNIATSTFLLKLEEMEKEIYKKSHESYKDTKRWMFKK

Sequence of entity 10 (D, K), FASTA

>9RHJ_10 DNA replication complex GINS protein SLD5 (chains D, K)
MDINIDDILAELDKETTAVDSTKITQGSSSTTHRDANTIVGSSLDLNDKTQIYVSPQQDF
SDLMKSWKNERCSPELLPYPHQLMKRLLNRISMQSQLIENISMGFLDMQNASNANPPMPN
ESKLPLLCMETELERLKFVIRSYIRCRLSKIDKFSLYLRQLNEDENSLISLTDLLSKDEI
KYHDTHSLIWLKLVNDSILKYMPEELQAINDTEGSVNMIDEPDWNKFVFIHVNGPPDGKW
NEDPLLQENEFGKPCYTVTIPDLKEEVELTIGSIYVMRYEVIRDLLRDDKVALI

Sequence of entity 11 (E, L), FASTA

>9RHJ_11 Cell division control protein 45 (chains E, L)
MYYGISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFKKQLVQSQIVP
IFGYSELRRHYSQLDDNINSLLLVGFGGVIDLEAFLEIDPQEYVIDTDEKSGEQSFRRDI
YVLDAHRPWNLDNIFGSQIIQCFDDGTVDDTLGEQKEAYYKLLELDEESGDDELSGDEND
NNGGDDEATDADEVTDEDYKDDDGDYKDDDETISNKRGNSSIGPNDLSKRKQRKKQIHEY
EGVLEEYYSQGTTVVNSISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYP
LLQDEVKRLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSLWNE
NGKKRLHKMFARMGIPLSTAQETWLYMDHSIKRELGIIFDKNLDRYGLQDIIRDGFVRTL
GYRGSISASEFVEALTALLEVGNSTDKDSVKINNDNNDDTDGEEEEDNSAQKLTNLRKRW
VSNFWLSWDALDDRKVELLNRGIQLAQDLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDG
PDLDLYRNPLTLLRLGNWLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGLD
TIHTKKPILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL

Sequence of entity 12 (F, M), FASTA

>9RHJ_12 DNA polymerase epsilon subunit B (chains F, M)
MFGSGNVLPVKIQPPLLRPLAYRVLSRKYGLSIKSDGLSALAEFVGTNIGANWRQGPATI
KFLEQFAAVWKQQERGLFIDQSGVKEVIQEMKEREKVEWSHEHPIQHEENILGRTDDDEN
NSDDEMPIAADSSLQNVSLSSPMRQPTERDEYKQPFKPESSKALDWRDYFKVINASQQQR
FSYNPHKMQFIFVPNKKQNGLGGIAGFLPDIEDKVQMFLTRYYLTNDRVMRNENFQNSDM
FNPLSSMVSLQNELSNTNRQQQSSSMSITPIKNLLGRDAQNFLLLGLLNKNFKGNWSLED
PSGSVEIDISQTIPTQGHYYVPGCMVLVEGIYYSVGNKFHVTSMTLPPGERREITLETIG
NLDLLGIHGISNNNFIARLDKDLKIRLHLLEKELTDHKFVILGANLFLDDLKIMTALSKI
LQKLNDDPPTLLIWQGSFTSVPVFASMSSRNISSSTQFKNNFDALATLLSRFDNLTENTT
MIFIPGPNDLWGSMVSLGASGTLPQDPIPSAFTKKINKVCKNVVWSSNPTRIAYLSQEIV
IFRDDLSGRFKRHRLEFPFNESEDVYTENDNMMSKDTDIVPIDELVKEPDQLPQKVQETR
KLVKTILDQGHLSPFLDSLRPISWDLDHTLTLCPIPSTMVLCDTTSAQFDLTYNGCKVIN
PGSFIHNRRARYMEYVPSSKKTIQEEIYI

Ligands and cofactors

ID	Name	Formula	Copies
MG	Magnesium ion	Mg	2
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	2
ZN	Zinc ion	Zn	10

Primary citation

Structure of the pre-initiation complex explains CMGE biogenesis. Puhringer, T., Canal, B., Palm, G. et al. Nature (2026) 655:1330-1338. DOI 10.1038/s41586-026-10657-7 · PubMed

Other PDB entries of the same protein (UniProt P29469 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7W8G 2.52 Å, Cryo-EM structure of MCM double hexamer
28VY 2.7 Å, sCMGE assembled on ARS1 DNA with Sld2 and RPA
8RIF 2.79 Å, Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
7V3V 2.9 Å, Cryo-EM structure of MCM double hexamer bound with DDK in State I
9TH8 2.9 Å, Cryo-EM structure of MCM2-7 DH bound to Sld3-Sld7, Cdc45 and DNA
7P30 3.0 Å, 3.0 A resolution structure of a DNA-loaded MCM double hexamer
8KG6 3.07 Å, Yeast replisome in state I
9RHL 3.1 Å, Phospho-DH bound by Sld3-MBD on ARS1 DNA
7PMK 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
7PMN 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
7PT6 3.2 Å, Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III
7V3U 3.2 Å, Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD

Browse structure collections

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