9WQL: GRM5-Gq Complex Structure

GRM5-Gq Complex Structure. Determined by electron microscopy at 3.2 Å resolution. Released 27 May 2026.

Method: Electron microscopy
Resolution: 3.2 Å
Organisms: Homo sapiens, Mus musculus
Chains: 6
Atoms: 19,387
Mol. weight: 297.56 kDa
Ligands: NAG, GGL, YKU, CLR
Released: 27 May 2026

Explore 9WQL in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9WQL contains 102 α-helices and 114 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	33-39	7	18
α-helix	46-55	10
β-strand	70-76	7	18
β-strand	79-85	7	18
α-helix	96-100	5
β-strand	105-111	7	18
α-helix	118-129	12
β-strand	138-144	7	18
α-helix	146-155	10
α-helix	160-163	4
α-helix	165-168	4
α-helix	178-179	2
α-helix	184-204	21
β-strand	211-215	5	18
α-helix	223-242	20

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	38-41	4
β-strand	47-51	5	19
β-strand	58-63	6	20
β-strand	69-74	6	20
β-strand	78-83	6	20
β-strand	88-94	7	20
β-strand	100-105	6	21
β-strand	111-116	6	21
β-strand	121-125	5	21
β-strand	135-139	5	21
β-strand	146-151	6	22
β-strand	156-161	6	22
β-strand	165-170	6	22
β-strand	175-181	7	22
β-strand	187-192	6	23
β-strand	198-203	6	23
β-strand	207-212	6	23
β-strand	218-223	6	23
β-strand	229-234	6	24
β-strand	240-245	6	24
β-strand	250-254	5	24
β-strand	259-264	6	24
α-helix	272	1
β-strand	273-278	6	25
β-strand	284-289	6	25
β-strand	293-298	6	25
β-strand	304-309	6	25
β-strand	315-320	6	19
β-strand	327-331	5	19
β-strand	336-339	4	19

Chain F: 6 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	26
β-strand	10-12	3	27
β-strand	17-25	9	26
α-helix	29-31	3
β-strand	33-39	7	27
β-strand	45-51	7	27
α-helix	53-55	3
β-strand	58-60	3	27
β-strand	68-73	6	26
β-strand	78-84	7	26
α-helix	88-90	3
β-strand	92-99	8	27
β-strand	110-111	2	27
β-strand	115-119	5	27
β-strand	140-141	2	28
β-strand	146-149	4	29
β-strand	155-161	7	28
β-strand	166	1	30
β-strand	172	1	30
β-strand	174-179	6	29
β-strand	186-190	5	29
β-strand	194-195	2	29
α-helix	196	1
β-strand	203-208	6	28
β-strand	211-216	6	28
α-helix	221-223	3
β-strand	225-231	7	29
α-helix	237	1
β-strand	239	1	29
β-strand	243-247	5	29

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	8-23	16
α-helix	30-44	15
α-helix	45-47	3
α-helix	53-55	3

Chain X: 39 helices, 29 β-strands

Element	Residues	Length	Sheet
β-strand	29-31	3	1
β-strand	35-41	7	1
β-strand	44	1	2
α-helix	45-48	4
β-strand	60	1	2
α-helix	62-67	6
α-helix	68-80	13
β-strand	91-97	7	1
α-helix	102-117	16
β-strand	143-147	5	1
α-helix	152-162	11
α-helix	163-165	3
β-strand	169-171	3	1
α-helix	177-180	4
β-strand	188-190	3	1
α-helix	195-208	14
β-strand	213-219	7	3
α-helix	222-237	16
β-strand	241-248	8	3
α-helix	254-265	12
β-strand	273-277	5	3
α-helix	280-293	14
β-strand	300-303	4	3
α-helix	311-314	4
α-helix	318-321	4
β-strand	325-329	5	3
α-helix	332-334	3
α-helix	335-341	7
α-helix	355-363	9
β-strand	366	1	4
β-strand	380	1	4
α-helix	381-382	2
α-helix	397-418	22
α-helix	427-429	3
α-helix	432-433	2
α-helix	434-443	10
β-strand	445-447	3	5
β-strand	453-455	3	5
β-strand	466-473	8	3
β-strand	479-488	10	3
β-strand	491-494	4	3
α-helix	496-499	4
α-helix	503-505	3
α-helix	513-515	3
β-strand	519-523	5	6
β-strand	532-536	5	6
α-helix	537-538	2
β-strand	541-545	5	7
β-strand	548-551	4	7
α-helix	552-553	2
β-strand	556-558	3	8
β-strand	565-567	3	8
α-helix	568-570	3
β-strand	571	1	9
α-helix	578-603	26
α-helix	608-611	4
α-helix	615-636	22
α-helix	638-639	2
α-helix	641-672	32
α-helix	684-685	2
α-helix	690-714	25
β-strand	719-722	4	9
β-strand	730-733	4	9
α-helix	737-760	24
α-helix	771-793	23
α-helix	798-817	20
α-helix	819-827	9
α-helix	829-831	3

Chain Y: 39 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	29-31	3	10
β-strand	35-41	7	10
β-strand	44	1	11
α-helix	45-48	4
α-helix	49-51	3
β-strand	60	1	11
α-helix	62-66	5
α-helix	67-80	14
β-strand	91-97	7	10
α-helix	102-113	12
α-helix	114-116	3
β-strand	143-147	5	10
α-helix	152-162	11
α-helix	163-165	3
β-strand	169-171	3	10
α-helix	177-180	4
β-strand	188-190	3	10
α-helix	195-208	14
β-strand	213-219	7	12
α-helix	222-237	16
β-strand	241-248	8	12
α-helix	254-266	13
β-strand	273-276	4	12
α-helix	280-293	14
β-strand	300-303	4	12
α-helix	311-314	4
α-helix	318-321	4
β-strand	325-329	5	12
α-helix	335-341	7
α-helix	355-363	9
α-helix	397-418	22
α-helix	427-429	3
α-helix	432-433	2
α-helix	434-443	10
β-strand	445-447	3	13
β-strand	453-455	3	13
β-strand	466-475	10	12
β-strand	478-488	11	12
β-strand	491-494	4	12
α-helix	496-499	4
α-helix	503-505	3
α-helix	506-508	3
α-helix	513-515	3
β-strand	519-523	5	14
β-strand	532-536	5	14
β-strand	541-545	5	15
β-strand	548-551	4	15
α-helix	552-553	2
β-strand	556-558	3	16
β-strand	565-567	3	16
α-helix	568-570	3
β-strand	571	1	17
α-helix	578-603	26
α-helix	608-611	4
α-helix	615-636	22
α-helix	638-639	2
α-helix	641-672	32
α-helix	690-714	25
β-strand	719-722	4	17
β-strand	730-733	4	17
α-helix	737-760	24
α-helix	770-792	23
α-helix	798-813	16
α-helix	814-818	5
α-helix	819-822	4
α-helix	823-827	5

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Metabotropic glutamate receptor 5	X, Y	protein	850	Homo sapiens	P41594 (AlphaFold model)
miniGq	A	protein	246	Homo sapiens	A0A590UJY2 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	80	Homo sapiens	P59768 (AlphaFold model)
scFv16	F	protein	260	Mus musculus

Sequence of entity 1 (X, Y), FASTA

>9WQL_1 Metabotropic glutamate receptor 5 (chains X, Y)
MWSHPQFEKGGGSGGGSGGSAWSHPQFEKGSQSSERRVVAHMPGDIIIGALFSVHHQPTV
DKVHERKCGAVREQYGIQRVEAMLHTLERINSDPTLLPNITLGCEIRDSCWHSAVALEQS
IEFIRDSLISSEEEEGLVRCVDGSSSSFRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQ
IAYSATSMDLSDKTLFKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGESGME
AFKDMSAKEGICIAHSYKIYSNAGEQSFDKLLKKLTSHLPKARVVACFCEGMTVRGLLMA
MRRLGLAGEFLLLGSDGWADRYDVTDGYQREAVGGITIKLQSPDVKWFDDYYLKLRPETN
HRNPWFQEFWQHRFQCRLEGFPQENSKYNKTCNSSLTLKTHHVQDSKMGFVINAIYSMAY
GLHNMQMSLCPGYAGLCDAMKPIDGRKLLESLMKTNFTGVSGDTILFDENGDSPGRYEIM
NFKEMGKDYFDYINVGSWDNGELKMDDDEVWSKKSNIIRSVCSEPCEKGQIKVIRKGEVS
CCWTCTPCKENEYVFDEYTCKACQLGSWPTDDLTGCDLIPVQYLRWGDPEPIAAVVFACL
GLLATLFVTVVFIIYRDTPVVKSSSRELCYIILAGICLGYLCTFCLIAKPKQIYCYLQRI
GIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFILICIQLGIIV
ALFIMEPPDIMHDYPSIREVYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFN
EAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKP
ERNVRSAFTT

Sequence of entity 2 (A), FASTA

>9WQL_2 miniGq (chains A)
MGSTVSAEDKAAAERSKMIDKNLREDGEKARRTLRLLLLGADNSGKSTIVKQMRILHGGS
GGSGGTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQE
ALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEP
GEDPRVTRAKYFIRKEFVDISTASGDGRHICYPHFTCAVDTENARRIFNDCKDIILQMNL
REYNLV

Sequence of entity 3 (B), FASTA

>9WQL_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (G), FASTA

>9WQL_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAILGSAGSAGSA

Sequence of entity 5 (F), FASTA

>9WQL_5 scFv16 (chains F)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAAASSEDLYFQ

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	8
GGL	Gamma-L-glutamic acid	C5 H9 N O4	2
YKU	3-cyano-N-(1,3-diphenyl-1H-pyrazol-5-yl)benzamide	C23 H16 N4 O	1
CLR	Cholesterol	C27 H46 O	7

Water and common crystallization additives (CL) are not listed.

Primary citation

G protein selectivity in group I metabotropic glutamate receptors. Lu, Y., Wen, T., Lu, X. et al. Sci Adv (2026) 12:eaee0044-eaee0044. DOI 10.1126/sciadv.aee0044 · PubMed

Other PDB entries of the same protein (UniProt P41594 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6FFI 2.2 Å, Crystal Structure of mGluR5 in complex with MMPEP at 2.2 A
9HC0 2.33 Å, Dark structure of the human metabotropic glutamate receptor 5 transmembrane domain bound…
3LMK 2.44 Å, Ligand Binding Domain of Metabotropoc glutamate receptor mGluR5 complexed with glutamate
7P2L 2.54 Å, thermostabilised 7TM domain of human mGlu5 receptor bound to photoswitchable ligand…
4OO9 2.6 Å, Structure of the human class C GPCR metabotropic glutamate receptor 5 transmembrane…
5CGD 2.6 Å, Structure of the human class C GPCR metabotropic glutamate receptor 5 transmembrane…
6FFH 2.65 Å, Crystal Structure of mGluR5 in complex with Fenobam at 2.65 A
8TAO 2.9 Å, Quis and CDPPB bound active mGlu5
9HC3 2.9 Å, Apo-state structure of the human metabotropic glutamate receptor 5 transmembrane domain…
8T8M 3.0 Å, Quis-bound intermediate mGlu5
8X0G 3.0 Å, Human FL Metabotropic glutamate receptor 5, mGlu5-5M with quisqualate, Acc conformation…
8X0B 3.1 Å, Human FL Metabotropic glutamate receptor 5, mGlu5-5M with Quisqualate and VU0424465

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