9WQM: GRM1-Gi Complex Structure

GRM1-Gi Complex Structure. Determined by electron microscopy at 3.3 Å resolution. Released 27 May 2026.

Method: Electron microscopy
Resolution: 3.3 Å
Organisms: Homo sapiens, Mus musculus
Chains: 6
Atoms: 19,309
Mol. weight: 313.52 kDa
Ligands: CLR, NAG, GGL, A1EX5
Released: 27 May 2026

Explore 9WQM in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9WQM contains 91 α-helices and 118 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	34-40	7	1
α-helix	46-54	9
β-strand	185-191	7	1
β-strand	194-200	7	1
α-helix	208-214	7
β-strand	220-226	7	1
α-helix	227-231	5
β-strand	233	1	2
β-strand	241	1	2
α-helix	242-254	13
α-helix	257-259	3
β-strand	263-269	7	1
α-helix	271-280	10
α-helix	283-285	3
α-helix	296-308	13
β-strand	319-323	5	1
α-helix	331-351	21

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-34	5
α-helix	38-41	4
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	121-125	5	5
β-strand	134-139	6	5
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	166-170	5	6
β-strand	175-180	6	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	218-223	6	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	259-264	6	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	293-298	6	9
β-strand	303-309	7	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	335-339	5	3

Chain F: 7 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	28
β-strand	10-12	3	29
β-strand	17-25	9	28
α-helix	29-31	3
β-strand	33-39	7	29
β-strand	45-51	7	29
α-helix	53-55	3
β-strand	58-60	3	29
β-strand	68-73	6	28
β-strand	78-84	7	28
α-helix	88-90	3
β-strand	92-99	8	29
β-strand	110-111	2	29
β-strand	115-119	5	29
α-helix	138-139	2
β-strand	140-141	2	30
β-strand	146-149	4	31
β-strand	155-161	7	30
β-strand	166	1	32
β-strand	172	1	32
β-strand	174-179	6	31
β-strand	185-190	6	31
β-strand	194-195	2	31
α-helix	196	1
β-strand	203-208	6	30
β-strand	211-216	6	30
α-helix	221-223	3
β-strand	225-231	7	31
α-helix	237	1
β-strand	239	1	31
β-strand	243-247	5	31

Chain G: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	6-23	18
α-helix	30-43	14

Chain X: 34 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	35-37	3
β-strand	39-41	3	10
β-strand	45-51	7	10
β-strand	54	1	11
α-helix	55-58	4
β-strand	70	1	11
α-helix	72-76	5
α-helix	77-90	14
β-strand	101-107	7	10
α-helix	112-123	12
α-helix	154-155	2
β-strand	156-160	5	10
α-helix	165-175	11
α-helix	176-178	3
β-strand	182-184	3	10
α-helix	190-193	4
β-strand	201-203	3	10
α-helix	208-221	14
β-strand	226-232	7	12
α-helix	235-251	17
β-strand	254-261	8	12
α-helix	267-278	12
β-strand	286-289	4	12
α-helix	293-306	14
β-strand	313-316	4	12
α-helix	324-327	4
α-helix	331-334	4
β-strand	338-342	5	12
α-helix	348-355	8
α-helix	368-376	9
β-strand	379	1	13
β-strand	393	1	13
α-helix	394	1
α-helix	410-431	22
α-helix	440-442	3
α-helix	447-455	9
β-strand	459-460	2	14
β-strand	466-467	2	14
β-strand	479-486	8	12
β-strand	492-501	10	12
β-strand	504-507	4	12
α-helix	526-528	3
β-strand	532-535	4	15
β-strand	546-549	4	15
β-strand	554-558	5	16
β-strand	561-564	4	16
α-helix	565-566	2
β-strand	569-571	3	17
β-strand	578-580	3	17
α-helix	581-583	3
β-strand	584	1	18
α-helix	591-616	26
α-helix	621-625	5
α-helix	628-649	22
α-helix	651-652	2
α-helix	654-686	33
α-helix	703-727	25
β-strand	732-735	4	18
β-strand	743-746	4	18
α-helix	750-773	24
α-helix	784-806	23
α-helix	812-840	29
α-helix	842-844	3

Chain Y: 35 helices, 29 β-strands

Element	Residues	Length	Sheet
β-strand	39-41	3	19
β-strand	45-51	7	19
β-strand	54	1	20
α-helix	55-58	4
α-helix	59-61	3
β-strand	70	1	20
α-helix	72-76	5
α-helix	77-91	15
β-strand	101-107	7	19
α-helix	112-123	12
β-strand	156-160	5	19
α-helix	165-175	11
α-helix	176-178	3
β-strand	182-184	3	19
α-helix	190-193	4
β-strand	201-203	3	19
α-helix	208-221	14
β-strand	226-232	7	21
α-helix	235-250	16
β-strand	254-261	8	21
α-helix	267-278	12
β-strand	286-289	4	21
α-helix	293-306	14
β-strand	313-316	4	21
α-helix	324-327	4
α-helix	331-334	4
β-strand	338-342	5	21
α-helix	348-355	8
α-helix	368-376	9
β-strand	379	1	22
β-strand	393	1	22
α-helix	394	1
α-helix	410-431	22
α-helix	440-442	3
α-helix	445-446	2
α-helix	447-456	10
β-strand	458-460	3	23
β-strand	466-468	3	23
β-strand	479-486	8	21
β-strand	492-501	10	21
β-strand	504-507	4	21
α-helix	509-511	3
α-helix	526-528	3
β-strand	532-535	4	24
β-strand	546-549	4	24
β-strand	554-556	3	25
β-strand	562-564	3	25
α-helix	565-566	2
β-strand	569-571	3	26
β-strand	578-580	3	26
α-helix	581-583	3
β-strand	584	1	27
α-helix	591-616	26
α-helix	621-625	5
α-helix	629-648	20
α-helix	654-685	32
α-helix	703-727	25
β-strand	732-735	4	27
β-strand	743-746	4	27
α-helix	750-773	24
α-helix	783-807	25
α-helix	811-835	25
α-helix	836-840	5
α-helix	842-844	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(i) subunit alpha-1	A	protein	354	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	80	Homo sapiens	P59768 (AlphaFold model)
Metabotropic glutamate receptor 1	X, Y	protein	869	Homo sapiens	Q13255 (AlphaFold model)
scFv16	F	protein	260	Mus musculus

Sequence of entity 1 (A), FASTA

>9WQM_1 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKNTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 2 (B), FASTA

>9WQM_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>9WQM_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAILGSAGSAGSA

Sequence of entity 4 (X, Y), FASTA

>9WQM_4 Metabotropic glutamate receptor 1 (chains X, Y)
DYKDDDDKTGASSQRSVARMDGDVIIGALFSVHHQPPAEKVPERKCGEIREQYGIQRVEA
MFHTLDKINADPVLLPNITLGSEIRDSCWHSSVALEQSIEFIRDSLISIRDEKDGINRCL
PDGQSLPPGRTKKPIAGVIGPGSSSVAIQVQNLLQLFDIPQIAYSATSIDLSDKTLYKYF
LRVVPSDTLQARAMLDIVKRYNWTYVSAVHTEGNYGESGMDAFKELAAQEGLCIAHSDKI
YSNAGEKSFDRLLRKLRERLPKARVVVCFCEGMTVRGLLSAMRRLGVVGEFSLIGSDGWA
DRDEVIEGYEVEANGGITIKLQSPEVRSFDDYFLKLRLDTNTRNPWFPEFWQHRFQCRLP
GHLLENPNFKRICTGNESLEENYVQDSKMGFVINAIYAMAHGLQNMHHALCPGHVGLCDA
MKPIDGSKLLDFLIKSSFIGVSGEEVWFDEKGDAPGRYDIMNLQYTEANRYDYVHVGTWH
EGVLNIDDYKIQMNKSGVVRSVCSEPCLKGQIKVIRKGEVSCCWICTACKENEYVQDEFT
CKACDLGWWPNADLTGCEPIPVRYLEWSNIESIIAIAFSCLGILVTLFVTLIFVLYRDTP
VVKSSSRELCYIILAGIFLGYVCPFTLIAKPTTTSCYLQRLLVGLSSAMCYSALVTKTNR
IARILAGSKKKICTRKPRFMSAWAQVIIASILISVQLTLVVTLIIMEPPMPILSYPSIKE
VYLICNTSNLGVVAPLGYNGLLIMSCTYYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAF
VPIYFGSNYKIITTCFAVSLSVTVALGCMFTPKMYIIIAKPERNVRSAFTTSDVVRMHVG
DGKLPCRSNTFLNIFRRKKAGAGNANSNG

Sequence of entity 5 (F), FASTA

>9WQM_5 scFv16 (chains F)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAAASSEDLYFQ

Ligands and cofactors

ID	Name	Formula	Copies
CLR	Cholesterol	C27 H46 O	6
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	8
GGL	Gamma-L-glutamic acid	C5 H9 N O4	2
A1EX5	N-[4-(trifluoromethyl)-1,3-oxazol-2-yl]-9H-xanthene-9-carboxamide	C18 H11 F3 N2 O3	1

Water and common crystallization additives (CL) are not listed.

Primary citation

G protein selectivity in group I metabotropic glutamate receptors. Lu, Y., Wen, T., Lu, X. et al. Sci Adv (2026) 12:eaee0044-eaee0044. DOI 10.1126/sciadv.aee0044 · PubMed

Other PDB entries of the same protein (UniProt P63096 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
3UMR 2.04 Å, Crystal structure of the G202D mutant of human G-alpha-i1
9P7Z 2.1 Å, NTSR1-Gi-NTS(8-13) Complex in the Canonical, AHD Open State (C-Open-Apo)
2OM2 2.2 Å, Crystal Structure Of Human G[alpha]i1 Bound To The Goloco Motif Of Rgs14
8YN9 2.3 Å, Cryo-EM structure of histamine H4 receptor in complex with histamine and Gi
9HYI 2.3 Å, DP81-bound serotonin 5-HT1A receptor - Gi Protein Complex
9O36 2.3 Å, CryoEM structure of mu-opioid receptor - Gi protein complex bound to fluornitrazene (FNZ)
9P80 2.3 Å, NTSR1-Gi-NTS(8-13) Complex in the Non-Canonical, AHD Open State (NC-Open-Apo)
8XXV 2.33 Å, Cryo-EM Structure of the Prostaglandin D2 Receptor 2-indomethacin Coupled to G Protein
3UMS 2.34 Å, Crystal structure of the G202A mutant of human G-alpha-i1
3ONW 2.38 Å, Structure of a G-alpha-i1 mutant with enhanced affinity for the RGS14 GoLoco motif.
20ZG 2.4 Å, Cryo-EM structure of the human neurotensin receptor 1 (hNTSR1)-Gi1 complex in the…

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