9XFI: Bavachalcone bound FFAR4-Giq complex

Cryo-EM structure of the Bavachalcone bound FFAR4-Giq complex. Determined by electron microscopy at 2.99 Å resolution. Released 16 Sept 2026.

Method: Electron microscopy
Resolution: 2.99 Å
Organism: Homo sapiens
Chains: 4
Atoms: 7,167
Mol. weight: 128.29 kDa
Ligands: A1EZZ
Released: 16 Sept 2026

Explore 9XFI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9XFI contains 28 α-helices and 36 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 13 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	38-70	33
α-helix	77-87	11
α-helix	88-91	4
α-helix	92-101	10
α-helix	108-144	37
α-helix	152-174	23
β-strand	179-182	4	1
β-strand	191-194	4	1
α-helix	201-212	12
α-helix	213-217	5
α-helix	218-243	26
α-helix	249-292	44
α-helix	300-311	12
α-helix	313-321	9
α-helix	323-328	6

Chain B: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	34-40	7	9
β-strand	185-191	7	9
β-strand	194-200	7	9
α-helix	208-210	3
α-helix	212-215	4
β-strand	220-226	7	9
α-helix	227-229	3
α-helix	242-254	13
β-strand	263-269	7	9
α-helix	271-280	10
α-helix	296-308	13
β-strand	319-322	4	9
α-helix	329-350	22

Chain C: 5 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	7-25	19
α-helix	30-34	5
α-helix	38-40	3
β-strand	47-52	6	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
α-helix	95	1
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	120-125	6	4
β-strand	134-140	7	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	175-181	7	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
α-helix	272	1
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	293-298	6	8
β-strand	304-309	6	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	335-339	5	2

Chain D: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	11-23	13
α-helix	30-43	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Free fatty acid receptor 4	A	protein	361	Homo sapiens	Q5NUL3 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	353	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	D	protein	71	Homo sapiens	P59768 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(q)…	B	protein	354	Homo sapiens	P50148 (AlphaFold model), P63096

Sequence of entity 1 (A), FASTA

>9XFI_1 Free fatty acid receptor 4 (chains A)
MSPECARAAGDAPLRSLEQANRTRFPFFSDVKGDHRLVLAAVETTVLVLIFAVSLLGNVC
ALVLVARRRRRGATACLVLNLFCADLLFISAIPLVLAVRWTEAWLLGPVACHLLFYVMTL
SGSVTILTLAAVSLERMVCIVHLQRGVRGPGRRARAVLLALIWGYSAVAALPLCVFFRVV
PQRLPGADQEISICTLIWPTIPGEISWDVSFVTLNFLVPGLVIVISYSKILQITKASRKR
LTVSLAYSESHQIRVSQQDFRLFRTLFLLMVSFFIMWSPIIITILLILIQNFKQDLVIWP
SLFFWVVAFTFANSALNPILYNMTLCRNEWKKIFCCFWFPEKGAILTDTSVKRNDLSIIS
G

Sequence of entity 2 (C), FASTA

>9XFI_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MHHHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRT
RRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAP
SGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCA
LWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHES
DINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLA
GYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (D), FASTA

>9XFI_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains D)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (B), FASTA

>9XFI_4 Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(q) subunit alpha (chains B)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTENIRFVFAAVKDTILQLNLKEYNLV

Ligands and cofactors

ID	Name	Formula	Copies
A1EZZ	(~{E})-3-(4-hydroxyphenyl)-1-[5-(3-methylbut-2-enyl)-2,4-bis(oxidanyl)phenyl]pr…	C20 H20 O4	1

Primary citation

G alpha q activation of free fatty acid receptor 4 suppresses metabolic dysfunction by disrupting Nr1h3-PPAR gamma axis. Kong, Y., Wang, J., Wang, Z. et al. Nat Commun (2026) 17. DOI 10.1038/s41467-026-75589-2 · PubMed

Other PDB entries of the same protein (UniProt Q5NUL3 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8G59 2.64 Å, Cryo-EM structure of the TUG891 bound GPR120-Giq complex
24XB 2.65 Å, Cryo-EM Structure of the Apo FFAR4-Gq Complex
24QH 2.74 Å, Cryo-EM Structure of the FFAR4-Gi complex with unknown density
8ID6 2.8 Å, Cryo-EM structure of the oleic acid bound GPR120-Gi complex
8IYS 2.95 Å, TUG891-bound FFAR4 in complex with Gq
8ID8 3.0 Å, Cryo-EM structure of the TUG891 bound GPR120-Gi complex
8ID9 3.0 Å, Cryo-EM structure of the eicosapentaenoic acid bound GPR120-Gi complex
8H4I 3.06 Å, DHA-bound FFAR4 in complex with Gs
8T3O 3.06 Å, Cryo-EM structure of the TUG-891 bound FFA4-Gq complex
8H4L 3.07 Å, DHA-bound FFAR4 in complex with Gq
8H4K 3.1 Å, GW9508-bound FFAR4 in complex with Gq
8ID3 3.1 Å, Cryo-EM structure of the 9-hydroxystearic acid bound GPR120-Gi complex

Browse structure collections

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