9Y1N: Cryo EM Structure of Full lengthmGluR8

Cryo EM Structure of Full lengthmGluR8 Bound to Agonist L-AP4 and PAM VU6005649 in complex with G proteins. Determined by electron microscopy at 6.18 Å resolution. Released 30 Sept 2026.

Method: Electron microscopy
Resolution: 6.18 Å
Organisms: Homo sapiens, Mus musculus
Chains: 6
Atoms: 17,725
Mol. weight: 304.46 kDa
Ligands: A1BWG, E7P
Released: 30 Sept 2026

Explore 9Y1N in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9Y1N contains 85 α-helices and 126 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 32 helices, 28 β-strands

Element	Residues	Length	Sheet
β-strand	45-50	6	1
β-strand	52	1	2
β-strand	55	1	3
β-strand	67	1	3
α-helix	73-88	16
β-strand	98-101	4	1
β-strand	104	1	2
α-helix	109-120	12
α-helix	121-123	3
β-strand	147-151	5	1
α-helix	156-166	11
α-helix	167-169	3
β-strand	173-175	3	1
β-strand	193-194	2	1
α-helix	197-198	2
α-helix	199-201	3
α-helix	203-213	11
β-strand	218-223	6	4
α-helix	226-242	17
β-strand	250-253	4	4
α-helix	261-269	9
β-strand	277-281	5	4
α-helix	284-296	13
β-strand	304	1	4
β-strand	307	1	4
α-helix	322-325	4
β-strand	327-332	6	5
α-helix	340-346	7
α-helix	359-367	9
α-helix	402-423	22
α-helix	433-435	3
α-helix	439-446	8
β-strand	452	1	6
β-strand	458	1	6
α-helix	463-465	3
β-strand	466	1	1
β-strand	472-477	6	5
β-strand	484-490	7	5
β-strand	491	1	7
β-strand	495	1	7
α-helix	518-521	4
β-strand	525-528	4	8
β-strand	536-539	4	8
β-strand	549	1	9
β-strand	552	1	9
β-strand	560-562	3	10
β-strand	569-571	3	10
α-helix	572-573	2
α-helix	585-606	22
α-helix	611-616	6
α-helix	620-633	14
α-helix	635-640	6
α-helix	646-681	36
α-helix	686-687	2
α-helix	692-716	25
α-helix	725-727	3
α-helix	746-770	25
α-helix	780-802	23
α-helix	810-832	23
α-helix	835-842	8

Chain B: 36 helices, 32 β-strands

Element	Residues	Length	Sheet
β-strand	42	1	11
β-strand	46-50	5	11
β-strand	55-57	3	12
α-helix	58-59	2
β-strand	64-67	4	12
α-helix	69-71	3
α-helix	73-86	14
β-strand	98-102	5	11
α-helix	109-116	8
β-strand	150	1	11
α-helix	156-166	11
β-strand	173	1	11
α-helix	181-184	4
β-strand	192	1	11
α-helix	197-198	2
α-helix	200-211	12
β-strand	217-218	2	13
β-strand	219-223	5	14
α-helix	226-242	17
β-strand	246-247	2	13
β-strand	252-253	2	14
α-helix	257-258	2
α-helix	262-269	8
β-strand	277-281	5	14
α-helix	284-296	13
β-strand	304-307	4	14
α-helix	316-318	3
α-helix	324-326	3
β-strand	328-333	6	14
α-helix	340-347	8
α-helix	359-366	8
β-strand	370	1	15
β-strand	383	1	15
α-helix	390-393	4
α-helix	402-423	22
α-helix	432-435	4
α-helix	439-447	9
β-strand	452	1	16
β-strand	458	1	16
β-strand	471-477	7	14
β-strand	484-491	8	14
β-strand	495	1	14
α-helix	520-521	2
β-strand	524-525	2	17
α-helix	527	1
β-strand	528	1	18
α-helix	529	1
β-strand	536	1	18
β-strand	539-540	2	17
β-strand	545-547	3	19
β-strand	553-555	3	19
α-helix	556-557	2
β-strand	560-562	3	20
β-strand	569-571	3	20
α-helix	572-573	2
β-strand	574	1	21
α-helix	582-605	24
α-helix	608-610	3
α-helix	612-615	4
α-helix	619-639	21
α-helix	647-681	35
α-helix	692-716	25
α-helix	725-728	4
β-strand	738	1	21
α-helix	746-769	24
α-helix	780-803	24
α-helix	811-832	22
α-helix	835-842	8

Chain C: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	7-24	18
α-helix	30-33	4
α-helix	36-44	9
α-helix	45-47	3

Chain D: 3 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	3	1	22
β-strand	6-7	2	23
β-strand	18-23	6	23
β-strand	25	1	22
β-strand	34-40	7	24
β-strand	44-51	8	24
α-helix	52	1
α-helix	62-64	3
β-strand	68-73	6	23
β-strand	78-83	6	23
β-strand	93-98	6	24
β-strand	115-116	2	24
β-strand	141	1	25
β-strand	146-149	4	26
β-strand	154-160	7	25
β-strand	166-167	2	27
β-strand	171-172	2	27
β-strand	174-175	2	28
β-strand	178-179	2	26
α-helix	184-185	2
β-strand	186	1	26
β-strand	189-190	2	29
β-strand	194-195	2	29
β-strand	203-208	6	25
β-strand	211-217	7	25
β-strand	225-227	3	26
β-strand	230-231	2	28
β-strand	238	1	28
β-strand	243-247	5	26

Chain E: 7 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	33-40	8	30
α-helix	46-55	10
β-strand	58	1	31
β-strand	184-187	4	30
β-strand	189	1	31
β-strand	190	1	30
β-strand	195-201	7	30
α-helix	211-214	4
β-strand	220-226	7	30
α-helix	230-232	3
α-helix	242-254	13
β-strand	263-264	2	30
β-strand	267-269	3	30
α-helix	296-309	14
β-strand	322	1	30
α-helix	330-350	21

Chain G: 3 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	8-29	22
α-helix	35-38	4
β-strand	52-57	6	32
β-strand	63-68	6	33
β-strand	74-79	6	33
β-strand	83-85	3	34
β-strand	87-88	2	33
β-strand	97-99	3	34
β-strand	105-110	6	35
β-strand	116-121	6	35
β-strand	125-130	6	35
β-strand	139-145	7	35
α-helix	150-151	2
β-strand	153-156	4	36
β-strand	162-165	4	36
β-strand	172-175	4	36
β-strand	180-184	5	36
β-strand	194-197	4	37
β-strand	203-207	5	37
β-strand	212-217	6	37
β-strand	223-228	6	37
β-strand	234	1	38
β-strand	238-239	2	38
β-strand	245-250	6	38
β-strand	254-259	6	38
β-strand	264-265	2	38
β-strand	269	1	38
β-strand	278-283	6	39
β-strand	289-294	6	39
β-strand	299-303	5	39
β-strand	323-325	3	32
β-strand	332-335	4	32
β-strand	340-344	5	32

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Metabotropic glutamate receptor 8	A, B	protein	841	Homo sapiens	O00222 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
scFv-14	D	protein	259	Mus musculus
Guanine nucleotide-binding protein G(i) subunit alpha-1	E	protein	354	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	G	protein	357	Homo sapiens	P62873 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>9Y1N_1 Metabotropic glutamate receptor 8 (chains A, B)
MKTIIALSYIFCLVFADYKDDDDAAAQEYAHSIRVDGDIILGGLFPVHAKGERGVPCGEL
KKEKGIHRLEAMLYAIDQINKDPDLLSNITLGVRILDTCSRDTYALEQSLTFVQALIEKD
ASDVKCANGDPPIFTKPDKISGVIGAAASSVSIMVANILRLFKIPQISYASTAPELSDNT
RYDFFSRVVPPDSYQAQAMVDIVTALGWNYVSTLASEGNYGESGVEAFTQISREIGGVCI
AQSQKIPREPRPGEFEKIIKRLLETPNARAVIMFANEDDIRRILEAAKKLNQSGHFLWIG
SDSWGSKIAPVYQQEEIAEGAVTILPKRASIDGFDRYFRSRTLANNRRNVWFAEFWEENF
GCKLGSHGKRNSHIKKCTGLERIARDSSYEQEGKVQFVIDAVYSMAYALHNMHKDLCPGY
IGLCPRMSTIDGKELLGYIRAVNFNGSAGTPVTFNENGDAPGRYDIFQYQITNKSTEYKV
IGHWTNQLHLKVEDMQWAHREHTHPASVCSLPCKPGERKKTVKGVPCCWHCERCEGYNYQ
VDELSCELCPLDQRPNMNRTGCQLIPIIKLEWHSPWAVVPVFVAILGIIATTFVIVTFVR
YNDTPIVRASGRELSYVLLTGIFLCYSITFLMIAAPDTIICSFRRVFLGLGMCFSYAALL
TKTNRIHRIFEQGKKSVTAPKFISPASQLVITFSLISVQLLGVFVWFVVDPPHIIIDYGE
QRTLDPEKARGVLKCDISDLSLICSLGYSILLMVTCTVYAIKTRGVPETFNEAKPIGFTM
YTTCIIWLAFIPIFFGTAQSAEKMYIQTTTLTVSMSLSASVSLGMLYMPKVYIIIFHPEQ
N

Sequence of entity 2 (C), FASTA

>9Y1N_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 3 (D), FASTA

>9Y1N_3 scFv-14 (chains D)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAAHHHHHHHH

Sequence of entity 4 (E), FASTA

>9Y1N_4 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains E)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 5 (G), FASTA

>9Y1N_5 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains G)
HHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRI
QMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTC
AYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGD
TTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFT
GHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGR
LLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Ligands and cofactors

ID	Name	Formula	Copies
A1BWG	(3P,8S)-3-(2,3-difluoro-4-methoxyphenyl)-2,5-dimethyl-7-(trifluoromethyl)pyrazo…	C16 H12 F5 N3 O	1
E7P	(2S)-2-amino-4-phosphonobutanoic acid	C4 H10 N O5 P	2

Primary citation

Structural basis of active state coupling of metabotropic glutamate receptor 8 to beta-arrestins. Marx, D.C., Huynh, K., Gonzalez-Hernandez, A.J. et al. Nat Commun (2026) 17. DOI 10.1038/s41467-026-77556-3 · PubMed

Other PDB entries of the same protein (UniProt O00222 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6BSZ 2.65 Å, Human mGlu8 Receptor complexed with glutamate
9N8Y 2.76 Å, Cryo EM Structure of Full Length mGluR8 Bound to Agonist L-AP4 and PAM VU6005649
6BT5 2.92 Å, Human mGlu8 Receptor complexed with L-AP4
6E5V 2.95 Å, human mGlu8 receptor amino terminal domain in complex with…
9MB9 2.97 Å, Cryo-EM structure of Gi-bound GPCR
9MBC 2.97 Å, Cryo-EM structure of agonist-bound GPCR
9N8Z 2.97 Å, Cryo EM Structure of Full Length mGluR8 Bound to Agonist L-AP4 and PAM VU6005649, class 2
9MBA 3.14 Å, Cryo-EM structure of complex of transducer-bound GPCR
9MBB 3.25 Å, Cryo-EM structure of antagonist-bound GPCR
9MBD 3.32 Å, Cryo-EM structure of Apo-GPCR
9Y1M 3.32 Å, Cryo EM Structure of Full Length mGluR8 in Complex with Beta-Arrestin-1 Bound to Agonist…

Browse structure collections

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