DNA-directed RNA polymerase III subunit RPC8 (RPC25) is a 212-residue protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P35718.
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The mean pLDDT of this model is 85.5 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 47% |
| 70 to 90 | Confident: backbone generally right | 47% |
| 50 to 70 | Low: treat with caution | 3% |
| Below 50 | Very low: often disordered regions | 3% |
What pLDDT means and how to read it
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNA. The RPC25/RPC8-RPC17/RPC9 subcomplex may bind Pol III transcripts emerging from the adjacent exit pore during elongation
Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. RPC25/RPC8 and RPC17/RPC9 form a Pol III subcomplex
Nucleus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 7Z0H | EM | 2.6 Å | G=1-212 |
| 7Z1O | EM | 2.7 Å | G=1-212 |
| 7Z31 | EM | 2.76 Å | G=1-212 |
| 7Z1L | EM | 2.8 Å | G=1-212 |
| 7Z30 | EM | 2.9 Å | G=1-212 |
| 7Z2Z | EM | 3.07 Å | G=1-212 |
| 2CKZ | X-ray | 3.2 Å | B/D=1-212 |
| 8BWS | EM | 3.2 Å | G=1-212 |
| 6TUT | EM | 3.25 Å | G=1-212 |
| 6EU3 | EM | 3.3 Å | G=1-212 |
| 6EU1 | EM | 3.4 Å | G=1-212 |
| 6EU2 | EM | 3.4 Å | G=1-212 |
| 7Z1M | EM | 3.4 Å | G=1-212 |
| 6F40 | EM | 3.7 Å | G=1-212 |
| 5FJ8 | EM | 3.9 Å | G=1-212 |
| 7Z1N | EM | 3.9 Å | G=1-212 |
| 6EU0 | EM | 4.0 Å | G=1-212 |
| 6CNB | EM | 4.1 Å | G=1-212 |
| 6CNC | EM | 4.1 Å | G=1-212 |
| 6F44 | EM | 4.2 Å | G=1-212 |
Showing 20 of 26 experimental structures (best resolution first).
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