1GT0: POU/HMG/DNA ternary complex

Crystal structure of a POU/HMG/DNA ternary complex. Determined by X-ray diffraction at 2.6 Å resolution. Released 30 Jan 2003.

Method: X-ray diffraction
Resolution: 2.6 Å
Organisms: MUS MUSCULUS, HOMO SAPIENS
Chains: 4
Atoms: 2,833
Mol. weight: 42.9 kDa
Released: 30 Jan 2003

Explore 1GT0 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1GT0 contains 12 α-helices and 0 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain C: 8 helices, 0 β-strands

Element	Residues	Length
α-helix	5-22	18
α-helix	27-38	12
α-helix	44-51	8
α-helix	57-75	19
α-helix	103-105	3
α-helix	110-122	13
α-helix	128-138	11
α-helix	142-156	15

Chain D: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	2-8	7
α-helix	9-22	14
α-helix	30-41	12
α-helix	46-66	21

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
5'-d(tptpcptptptpgptptptp* gpgpap* tpgpcptpapaptpgpgpgpa)-3'	A	DNA	24	MUS MUSCULUS
5'-d(aptpcpcpcpaptptpapgp* cpaptpcpcpapapapcpapapapgp*a)-3'	B	DNA	24	MUS MUSCULUS
Octamer-binding transcription factor 1	C	protein	159	HOMO SAPIENS	P14859 (AlphaFold model)
Transcription factor sox-2	D	protein	80	MUS MUSCULUS	P48432 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>1GT0_1 5'-D(*TP*TP*CP*TP*TP*TP*GP*TP*TP*TP* GP*GP*AP* TP*GP*CP*TP*AP*AP*TP*GP*GP*GP*A)-3' (chains A)
TTCTTTGTTTGGATGCTAATGGGA

Sequence of entity 2 (B), FASTA

>1GT0_2 5'-D(*AP*TP*CP*CP*CP*AP*TP*TP*AP*GP* CP*AP*TP*CP*CP*AP*AP*AP*CP*AP*AP*AP*GP*A)-3' (chains B)
ATCCCATTAGCATCCAAACAAAGA

Sequence of entity 3 (C), FASTA

>1GT0_3 OCTAMER-BINDING TRANSCRIPTION FACTOR 1 (chains C)
EEPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNM
SKLKPLLEKWLNDAENLSSDSSLSSPSALNSPGIEGLSRRRKKRTSIETNIRVALEKSFL
ENQKPTSEEITMIADQLNMEKEVIRVWFSNRRQKEKRIN

Sequence of entity 4 (D), FASTA

>1GT0_4 TRANSCRIPTION FACTOR SOX-2 (chains D)
DRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLR
ALHMKEHPDYKYRPRRKTKT

Primary citation

Crystal Structure of a POU/Hmg/DNA Ternary Complex Suggests Differential Assembly of Oct4 and Sox2 on Two Enhancers. Remenyi, A., Lins, K., Nissen, L.J. et al. Genes Dev (2003) 17:2048. DOI 10.1101/GAD.269303 · PubMed

Other PDB entries of the same protein (UniProt P14859 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1E3O 1.9 Å, Crystal structure of Oct-1 POU dimer bound to MORE
1HF0 2.7 Å, Crystal structure of the DNA-binding domain of Oct-1 bound to DNA as a dimer
1OCT 3.0 Å, Crystal structure of the oct-1 pou domain bound to an octamer site: DNA recognition with…
1CQT 3.2 Å, Crystal structure of a ternary complex containing an oca-B peptide, the oct-1 pou…
1O4X Ternary complex of the DNA binding domains of the OCT1 and SOX2 transcription factors…
1POG Solution structure of the oct-1 pou-homeo domain determined by NMR and restrained…
1POU The solution structure of the oct-1 pou-specific domain reveals a striking similarity to…

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