3SN6: Beta2 adrenergic receptor-Gs protein complex

Crystal structure of the beta2 adrenergic receptor-Gs protein complex. Determined by X-ray diffraction at 3.2 Å resolution. Released 20 Jul 2011.

Method: X-ray diffraction
Resolution: 3.2 Å
Organisms: Bos taurus, Rattus norvegicus, Enterobacteria phage T4
Chains: 5
Atoms: 10,274
Mol. weight: 164.49 kDa
Ligands: P0G
Released: 20 Jul 2011

Explore 3SN6 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3SN6 contains 57 α-helices and 51 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 22 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	12-39	28
β-strand	41-46	6	1
α-helix	53-56	4
α-helix	89-109	21
α-helix	116-119	4
α-helix	122-124	3
α-helix	125-134	10
α-helix	144-155	12
α-helix	157-163	7
α-helix	176-180	5
α-helix	182-186	5
α-helix	194-197	4
α-helix	198-200	3
β-strand	209-214	6	1
β-strand	217-223	7	1
α-helix	224-226	3
α-helix	234-237	4
β-strand	243-249	7	1
α-helix	250-253	4
α-helix	265-278	14
β-strand	286-292	7	1
α-helix	294-303	10
α-helix	308-311	4
α-helix	313-315	3
α-helix	326-327	2
α-helix	332-352	21
β-strand	359-363	5	1
α-helix	372-390	19

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	3-24	22
α-helix	30-33	4
β-strand	47-52	6	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	135-139	5	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	175-180	6	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	259-264	6	7
α-helix	272	1
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	335-339	5	2

Chain G: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	6-23	18
α-helix	30-44	15
α-helix	45-47	3
α-helix	53-55	3

Chain N: 2 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	11
β-strand	11-12	2	12
β-strand	18-25	8	11
α-helix	29-31	3
β-strand	34-39	6	13
β-strand	46-51	6	13
β-strand	58-60	3	13
β-strand	68-73	6	11
β-strand	78-83	6	11
α-helix	88-90	3
β-strand	92-98	7	13
β-strand	118	1	13
β-strand	122-124	3	13
β-strand	125-126	2	12

Chain R: 26 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	1003-1010	8
β-strand	1016	1	9
β-strand	1018-1019	2	10
β-strand	1025-1027	3	10
β-strand	1031-1034	4	10
α-helix	1039-1050	12
β-strand	1057	1	9
α-helix	1060-1080	21
α-helix	1085-1090	6
α-helix	1093-1105	13
α-helix	1107-1112	6
α-helix	1115-1122	8
α-helix	1126-1134	9
α-helix	1137-1141	5
α-helix	1143-1155	13
α-helix	32-60	29
α-helix	62-64	3
α-helix	67-80	14
α-helix	82-86	5
α-helix	87-96	10
α-helix	104-136	33
α-helix	138-144	7
α-helix	147-163	17
α-helix	166-170	5
α-helix	180-186	7
α-helix	197-204	8
α-helix	205-209	5
α-helix	210-236	27
α-helix	266-298	33
α-helix	305-326	22
α-helix	330-337	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	380	Bos taurus	P04896 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	351	Rattus norvegicus	P54311 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	68	Bos taurus	P63212 (AlphaFold model)
Endolysin,Beta-2 adrenergic receptor	R	protein	514	Enterobacteria phage T4, Homo sapiens	P00720, P07550
Camelid antibody VHH fragment	N	protein	138	Lama glama

Sequence of entity 1 (A), FASTA

>3SN6_1 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQM
RILHVNGFNGDSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSV
MNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDYVPSD
QDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYN
MVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPE
FARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRR
VFNDCRDIIQRMHLRQYELL

Sequence of entity 2 (B), FASTA

>3SN6_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>3SN6_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFC

Sequence of entity 4 (R), FASTA

>3SN6_4 Endolysin,Beta-2 adrenergic receptor (chains R)
DYKDDDDAENLYFQGNIFEMLRIDEGLRLKIYKDTEGYYTIGIGHLLTKSPSLNAAKSEL
DKAIGRNTNGVITKDEAEKLFNQDVDAAVRGILRNAKLKPVYDSLDAVRRAALINMVFQM
GETGVAGFTNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTGTWDAYAADEV
WVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGA
AHILTKTWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKAR
VIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYAEETCCDFFTNQAYAIASSIVSFYVP
LVIMVFVYSRVFQEAKRQLQKIDKSEGRFHVQNLSQVEQDGRTGHGLRRSSKFCLKEHKA
LKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPD
FRIAFQELLCLRRSSLKAYGNGYSSNGNTGEQSG

Sequence of entity 5 (N), FASTA

>3SN6_5 Camelid antibody VHH fragment (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Ligands and cofactors

ID	Name	Formula	Copies
P0G	8-[(1R)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydro…	C21 H26 N2 O4	1

Primary citation

Crystal structure of the beta2 adrenergic receptor-Gs protein complex. Rasmussen, S.G., DeVree, B.T., Zou, Y. et al. Nature (2011) 477:549-555. DOI 10.1038/nature10361 · PubMed

Other PDB entries of the same protein (UniProt P04896 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1AZS 2.3 Å, Complex of gs-alpha with the catalytic domains of mammalian adenylyl cyclase
1AZT 2.3 Å, Gs-alpha complexed with GTP-gamma-S
9IJE 2.34 Å, Epinephrine-activated human beta3 adrenergic receptor
1CUL 2.4 Å, Complex of gs-alpha with the catalytic domains of mammalian adenylyl cyclase: complex…
1CS4 2.5 Å, Complex of gs-alpha with the catalytic domains of mammalian adenylyl cyclase: complex…
8DCS 2.5 Å, Cryo-EM structure of cyanopindolol-bound beta1-adrenergic receptor in complex with…
7JJO 2.6 Å, Structural Basis of the Activation of Heterotrimeric Gs-protein by Isoproterenol-bound…
8DCR 2.6 Å, Cryo-EM structure of dobutamine-bound beta1-adrenergic receptor in complex with…
7X8R 2.61 Å, Cryo-EM structure of the Boc5-bound hGLP-1R-Gs complex
3C14 2.68 Å, Complex of GS-Alpha with the Catalytic Domains of Mammalian Adenylyl Cyclase: Complex…
7VQX 2.74 Å, Cryo-EM structure of human vasoactive intestinal polypeptide receptor 2 (VIP2R) in…
8X9T 2.75 Å, Identification, structure and agonist design of an androgen membrane receptor

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3SN6 is part of these collections:

GPCRs and cell signaling

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