4AQW: Tubulin alpha-1D chain

Model of human kinesin-5 motor domain (1II6, 3HQD) and mammalian tubulin heterodimer (1JFF) docked into the 9.5-angstrom cryo-EM map of microtubule-bound kinesin-5 motor domain in the rigor state. Determined by electron microscopy at 9.5 Å resolution. Released 21 Nov 2012.

Method

Electron microscopy

Resolution

9.5 Å

Organisms

BOS TAURUS, HOMO SAPIENS

Chains

3

Atoms

1,311

Mol. weight

143.66 kDa

Ligands

GTP, MG, GDP, TA1

Released

21 Nov 2012

Explore 4AQW in 3D Show helices and sheets RCSB PDB PDBe

Molecules and chains

>4AQW_1 TUBULIN ALPHA-1D CHAIN (chains A)
MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGK
HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLD
RIRKLADQCTGLQGFSVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTA
VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA
SLRFDGALNVDLTEFQTNLVPYPRGHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN
QMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYEPP
TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDMAALEKDYEEVGVDSVEGEGEEEEGEEY

>4AQW_2 TUBULIN BETA-2B CHAIN (chains B)
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYV
PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV
RKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVV
EPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCL
RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMM
AACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRG
LKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS
EYQQYQDATADEQGEFEEEEGEDEA

>4AQW_3 KINESIN-LIKE PROTEIN KIF11 (chains C)
MASQPNSSAKKKEEKGKNIQVVVRVRPFNLAERKASAHSIVESDPVRKEVSVRTGGLADK
SSRKTYTFDMVFGASTKQIDVYRSVVAPILDEVIMGYNATIFAYGQTGTGKTFTMEGERS
PNEEYCWEEDPLAGIIPRTLHQIFEKLTDNGTEFSVKVSLLEIYNEELFDLLNPSSDVSE
RLQMFDDPRNKRGVIIKGLEEITVHNKDEVYQILEKGAAKRTTAATLMNAYSSRSHSVFS
VTIHMKETTIDGEELVKIGKLNLVDLAGSENIGRSGAVDKRAREAGNINQSLLTLGRVIT
ALVERTPHVPYRESKLTRILQDSLGGRTRTSIIATISPASLNLEETLSTLEYAHRAKNIL
NKPEVNQHHHHHH

Ligands and cofactors

Primary citation

The Structural Basis of Force Generation by the Mitotic Motor Kinesin-5. Goulet, A., Behnke-Parks, W.M., Sindelar, C.V. et al. J Biol Chem (2012) 287:44654. DOI 10.1074/JBC.M112.404228 · PubMed

Related structures

Other PDB entries of the same protein (UniProt Q2HJ86 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 1TVK 2.89 Å, The binding mode of epothilone A on a,b-tubulin by electron crystallography
• 7RRO 3.4 Å, Structure of the 48-nm repeat doublet microtubule from bovine tracheal cilia
• 9CPB 3.52 Å, Atomic model of bovine Fallopian tube cilia doublet microtubule (48-nm periodicity)
• 1SA0 3.58 Å, Tubulin-colchicine: stathmin-like domain complex
• 1Z2B 4.1 Å, Tubulin-colchicine-vinblastine: stathmin-like domain complex
• 1SA1 4.2 Å, Tubulin-podophyllotoxin: stathmin-like domain complex
• 2XRP 8.2 Å, Human Doublecortin N-DC Repeat (1MJD) and Mammalian Tubulin (1JFF and 3HKE) Docked into…
• 4ATX 8.2 Å, Rigor kinesin motor domain with an ordered neck-linker, docked on tubulin dimer,…
• 4ATU 8.3 Å, Human doublecortin N-DC repeat plus linker, and tubulin (2XRP) docked into an 8A cryo-EM…
• 3IZ0 8.6 Å, Human Ndc80 Bonsai Decorated Microtubule
• 4CK6 9.2 Å, Pseudo-atomic model of microtubule-bound human kinesin-5 motor domain in the ADP.AlFx…
• 4CK7 9.2 Å, Pseudo-atomic model of microtubule-bound human kinesin-5 motor domain in presence of…

Browse structure collections

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