4BU0: Rad4 BRCT1,2

Crystal structure of Rad4 BRCT1,2 in complex with a Crb2 phosphopeptide. Determined by X-ray diffraction at 1.5 Å resolution. Released 9 Oct 2013.

Method: X-ray diffraction
Resolution: 1.5 Å
Organism: SCHIZOSACCHAROMYCES POMBE
Chains: 3
Atoms: 2,146
Mol. weight: 25.16 kDa
Released: 9 Oct 2013

Explore 4BU0 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4BU0 contains 12 α-helices and 15 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	11-15	5	1
α-helix	19-31	13
β-strand	35-36	2	1
β-strand	40-41	2	2
β-strand	46-49	4	1
α-helix	55-63	9
β-strand	68-70	3	1
α-helix	74-83	10
β-strand	98	1	1
α-helix	99-100	2
β-strand	106-110	5	3
β-strand	114	1	4
α-helix	116-126	11
α-helix	129	1
β-strand	130-131	2	3
β-strand	135	1	5
β-strand	141-144	4	3
α-helix	150-157	8
β-strand	161-163	3	3
α-helix	166-174	9
α-helix	177-179	3
α-helix	180-183	4

Chain B: 1 helix, 1 β-strand

Element	Residues	Length	Sheet
β-strand	182-184	3	2
α-helix	185-188	4

Chain C: 1 helix, 2 β-strands

Element	Residues	Length	Sheet
β-strand	184	1	5
α-helix	185-187	3
β-strand	192	1	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
S-M checkpoint control protein RAD4	A	protein	186	SCHIZOSACCHAROMYCES POMBE	P32372 (AlphaFold model)
DNA repair protein RHP9	B, C	protein	16	SCHIZOSACCHAROMYCES POMBE	P87074 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>4BU0_1 S-M CHECKPOINT CONTROL PROTEIN RAD4 (chains A)
MGSSKPLKGFVICCTSIDLKQRTEISTKATKLGAAYRSDFTKDVTHLIAGDFDTPKYKFA
AKSRPDIKIMSSEWIPVLYESWVQGEDLDDGLLVDKHLLPTLFKCRVCLTNIGQPERSRI
ENYVLKHGGTFCPDLTRDVTHLIAGTSSGRKYEYALKWKINVVCVEWLWQSIQRNAVLEP
QYFQLD

Sequence of entity 2 (B, C), FASTA

>4BU0_2 DNA REPAIR PROTEIN RHP9 (chains B, C)
GYGEVLVPETVAQHRT

Primary citation

Phosphorylation-Dependent Assembly and Coordination of the DNA Damage Checkpoint Apparatus by Rad4(Topbp1.). Qu, M., Rappas, M., Wardlaw, C.P. et al. Mol Cell (2013) 51:723. DOI 10.1016/J.MOLCEL.2013.08.030 · PubMed

Other PDB entries of the same protein (UniProt P32372 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6HM4 1.77 Å, Crystal structure of Rad4 BRCT1,2 in complex with a Mdb1 phosphopeptide
6HM3 1.77 Å, Crystal structure of Rad4 BRCT1,2 in complex with a Sld3 phosphopeptide
4BMC 1.98 Å, Crystal structure of s.pombe Rad4 BRCT1,2
4BU1 2.1 Å, Crystal structure of Rad4 BRCT1,2 in complex with a Crb2 phosphopeptide
4BMD 2.5 Å, Crystal structure of S.pombe Rad4 BRCT3,4

Browse structure collections

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