4DNV: W285F mutant of UVB-resistance protein UVR8

Crystal structure of the W285F mutant of UVB-resistance protein UVR8. Determined by X-ray diffraction at 2.0 Å resolution. Released 7 Mar 2012.

Method: X-ray diffraction
Resolution: 2.0 Å
Organism: Arabidopsis thaliana
Chains: 4
Atoms: 11,846
Mol. weight: 159.35 kDa
Released: 7 Mar 2012

Explore 4DNV in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4DNV contains 37 α-helices and 114 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	13-15	3
β-strand	17-22	6	1
β-strand	26-31	6	1
β-strand	35-40	6	1
β-strand	55-60	6	1
α-helix	62-64	3
β-strand	71-75	5	2
β-strand	78-83	6	2
β-strand	88-93	6	2
α-helix	96-98	3
β-strand	108-113	6	2
α-helix	115-117	3
β-strand	122-127	6	3
β-strand	131-136	6	3
β-strand	141-145	5	3
β-strand	160-165	6	3
α-helix	167-169	3
β-strand	174-179	6	4
β-strand	183-188	6	4
β-strand	193-197	5	4
β-strand	212-217	6	4
β-strand	226-231	6	5
β-strand	235-240	6	5
β-strand	245-249	5	5
β-strand	264-269	6	5
α-helix	271-273	3
α-helix	277	1
β-strand	278-283	6	6
β-strand	287-292	6	6
β-strand	297-301	5	6
β-strand	316-322	7	6
α-helix	325-327	3
α-helix	328-329	2
β-strand	330-335	6	7
β-strand	339-344	6	7
β-strand	349-353	5	7
β-strand	368-373	6	7
α-helix	375-377	3

Chain B: 9 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	15-16	2
β-strand	17-22	6	8
β-strand	26-31	6	8
β-strand	35-40	6	8
β-strand	55-60	6	8
α-helix	62-64	3
β-strand	69-74	6	9
β-strand	78-83	6	9
β-strand	88-93	6	9
α-helix	96-98	3
β-strand	108-113	6	9
α-helix	115-117	3
β-strand	122-127	6	10
β-strand	131-136	6	10
β-strand	141-145	5	10
β-strand	160-165	6	10
α-helix	167-169	3
β-strand	174-179	6	11
β-strand	183-188	6	11
β-strand	193-197	5	11
β-strand	212-217	6	11
α-helix	224-225	2
β-strand	226-231	6	12
β-strand	235-240	6	12
β-strand	245-249	5	12
β-strand	264-269	6	12
α-helix	271-273	3
β-strand	278-283	6	13
β-strand	287-292	6	13
β-strand	297-301	5	13
β-strand	313	1	14
β-strand	316-321	6	13
α-helix	325-327	3
α-helix	328-329	2
β-strand	330-335	6	15
β-strand	339-344	6	15
β-strand	349-353	5	15
β-strand	368-373	6	15

Chain C: 9 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	14-16	3
β-strand	17-22	6	14
β-strand	26-31	6	14
β-strand	35-40	6	14
β-strand	55-60	6	14
α-helix	62-64	3
β-strand	69-74	6	16
β-strand	78-83	6	16
β-strand	88-93	6	16
α-helix	96-98	3
β-strand	108-113	6	16
α-helix	115-117	3
β-strand	122-127	6	17
β-strand	131-136	6	17
β-strand	141-145	5	17
β-strand	160-165	6	17
α-helix	167-169	3
β-strand	174-179	6	18
β-strand	183-188	6	18
β-strand	193-197	5	18
β-strand	212-217	6	18
β-strand	226-231	6	19
β-strand	235-240	6	19
β-strand	245-249	5	19
β-strand	264-269	6	19
α-helix	271-273	3
β-strand	278-283	6	20
β-strand	287-292	6	20
β-strand	297-301	5	20
β-strand	316-322	7	20
α-helix	325-327	3
α-helix	328-329	2
β-strand	330-335	6	21
β-strand	339-344	6	21
β-strand	349-353	5	21
β-strand	368-373	6	21
α-helix	375-377	3

Chain D: 9 helices, 29 β-strands

Element	Residues	Length	Sheet
β-strand	17-22	6	22
β-strand	26-31	6	22
β-strand	35-40	6	22
β-strand	55-60	6	22
α-helix	62-64	3
β-strand	69-74	6	23
β-strand	78-83	6	23
β-strand	88-93	6	23
α-helix	96-98	3
β-strand	108-113	6	23
α-helix	115-117	3
β-strand	122-127	6	24
β-strand	131-136	6	24
β-strand	141-145	5	24
β-strand	160-165	6	24
α-helix	167-169	3
β-strand	174-179	6	25
β-strand	183-188	6	25
β-strand	193-197	5	25
β-strand	212-217	6	25
β-strand	226-231	6	26
β-strand	235-240	6	26
β-strand	245-249	5	26
β-strand	264-269	6	26
α-helix	271-273	3
β-strand	278-283	6	27
β-strand	287-292	6	27
β-strand	297-301	5	27
β-strand	313	1	1
β-strand	316-321	6	27
α-helix	322-323	2
α-helix	325-327	3
α-helix	328-329	2
β-strand	330-335	6	28
β-strand	339-344	6	28
β-strand	348-353	6	28
β-strand	368-373	6	28
α-helix	375-377	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
AT5g63860/MGI19_6	A, B, C, D	protein	370	Arabidopsis thaliana	Q9FN03 (AlphaFold model)

Sequence of entity 1 (A, B, C, D), FASTA

>4DNV_1 AT5g63860/MGI19_6 (chains A, B, C, D)
APPRKVLIISAGASHSVALLSGDIVCSWGRGEDGQLGHGDAEDRPSPTQLSALDGHQIVS
VTCGADHTVAYSQSGMEVYSWGWGDFGRLGHGNSSDLFTPLPIKALHGIRIKQIACGDSH
CLAVTMEGEVQSWGRNQNGQLGLGDTEDSLVPQKIQAFEGIRIKMVAAGAEHTAAVTEDG
DLYGWGWGRYGNLGLGDRTDRLVPERVTSTGGEKMSMVACGWRHTISVSYSGALYTYGWS
KYGQLGHGDLEDHLIPHKLEALSNSFISQISGGFRHTMALTSDGKLYGWGWNKFGQVGVG
NNLDQCSPVQVRFPDDQKVVQVSCGWRHTLAVTERNNVFAWGRGTNGQLGIGESVDRNFP
KIIEALSVDG

Primary citation

Structural basis of ultraviolet-B perception by UVR8. Wu, D., Hu, Q., Yan, Z. et al. Nature (2012) 484:214-219. DOI 10.1038/nature10931 · PubMed

Other PDB entries of the same protein (UniProt Q9FN03 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6QTS 1.11 Å, Crystal structure of a mutant Arabidopsis WD40 domain in complex with a photoreceptor
6QTQ 1.3 Å, Crystal structure of an Arabidopsis WD40 domain in complex with photoreceptor
6XZL 1.39 Å, Arabidopsis UV-B photoreceptor UVR8 mutant D96N D107N
4NBM 1.61 Å, Crystal structure of UVB photoreceptor UVR8 and light-induced structural changes at 180K
4NAA 1.67 Å, Crystal structure of UVB photoreceptor UVR8 from Arabidopsis thaliana and UV-induced…
4D9S 1.7 Å, Crystal structure of Arabidopsis thaliana UVR8 (UV Resistance locus 8)
4NC4 1.75 Å, Crystal structure of photoreceptor AtUVR8 mutant W285F and light-induced structural…
6XZN 1.75 Å, Arabidopsis UV-B photoreceptor UVR8 mutant G101S W285A
4DNU 1.76 Å, Crystal structure of the W285A mutant of UVB-resistance protein UVR8
4DNW 1.77 Å, Crystal structure of UVB-resistance protein UVR8
6DD7 2.0 Å, Crystal structure of plant UVB photoreceptor UVR8 from in situ serial Laue diffraction
8GQE 2.0 Å, Crystal structure of the W285A mutant of UVR8 in complex with RUP2

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