4NE6: Human MHF1-MHF2 complex

Human MHF1-MHF2 complex. Determined by X-ray diffraction at 2.1 Å resolution. Released 25 Dec 2013.

Method: X-ray diffraction
Resolution: 2.1 Å
Organism: Homo sapiens
Chains: 4
Atoms: 2,745
Mol. weight: 38.37 kDa
Released: 25 Dec 2013

Explore 4NE6 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4NE6 contains 15 α-helices and 8 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 4 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	15-38	24
β-strand	41-42	2	1
α-helix	44-71	28
β-strand	76-77	2	2
α-helix	79-85	7
α-helix	90-104	15

Chains B and D: 3 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	12-20	9
β-strand	29-30	2	2
α-helix	32-60	29
β-strand	64-65	2	1
α-helix	67-80	14

Chain C: 5 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	15-38	24
β-strand	41-42	2	3
α-helix	44-71	28
β-strand	76-77	2	4
α-helix	79-85	7
α-helix	86-88	3
α-helix	90-104	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Centromere protein S	A, C	protein	93	Homo sapiens	Q8N2Z9 (AlphaFold model)
Centromere protein X	B, D	protein	74	Homo sapiens	A8MT69 (AlphaFold model)

Sequence of entity 1 (A, C), FASTA

>4NE6_1 Centromere protein S (chains A, C)
SYQQRLKAAVHYTVGCLCEEVALDKAMQFSKQTIAAISELTFRQCENFAKDLEMFARHAK
RTTINTEDVKLLARRSNSLLKYITDKSEEIAQA

Sequence of entity 2 (B, D), FASTA

>4NE6_2 Centromere protein X (chains B, D)
SGFRKELVSRLLHLHFKDDKTKVSGDALQLMVELLKVFVVEAAVRGVRQAQAEDALRVDV
DQLEKVLPQLLLDF

Primary citation

The MHF complex senses branched DNA by binding a pair of crossover DNA duplexes. Zhao, Q., Saro, D., Sachpatzidis, A. et al. Nat Commun (2014) 5:2987-2987. DOI 10.1038/ncomms3987 · PubMed

Other PDB entries of the same protein (UniProt Q8N2Z9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4NE3 1.8 Å, Human MHF1-MHF2 complex
4E45 2.0 Å, Crystal structure of the hMHF1/hMHF2 Histone-Fold Tetramer in Complex with Fanconi…
4E44 2.1 Å, Crystal structure of the hMHF1/hMHF2 Histone-Fold Tetramer
4DRA 2.41 Å, Crystal structure of MHF complex
4NE5 2.5 Å, Human MHF1-MHF2 complex
4DRB 2.63 Å, The crystal structure of FANCM bound MHF complex
28OP 2.7 Å, Structure of the human inner kinetochore CCAN and CENP-C bound to DNA
7R5S 2.83 Å, Structure of the human CCAN bound to alpha satellite DNA
7XHO 3.29 Å, Structure of human inner kinetochore CCAN complex
9TAW 3.54 Å, Structure of the human inner kinetochore CCAN bound to DNA
7XHN 3.71 Å, Structure of human inner kinetochore CCAN-DNA complex
9TAX 4.5 Å, Structure of the human inner kinetochore CCAN bound to a mono-CENP-A nucleosome

Browse structure collections

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