4Z94: Actin, alpha skeletal muscle

Actin Complex With a Chimera of Tropomodulin-1 and Leiomodin-1 Actin-Binding Site 2. Determined by X-ray diffraction at 2.4 Å resolution. Released 21 Oct 2015.

Method: X-ray diffraction
Resolution: 2.4 Å
Organisms: Oryctolagus cuniculus, Homo sapiens
Chains: 2
Atoms: 5,582
Mol. weight: 79.27 kDa
Ligands: CA, ATP
Released: 21 Oct 2015

Explore 4Z94 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4Z94 contains 36 α-helices and 33 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 23 helices, 21 β-strands

Element	Residues	Length	Sheet
α-helix	6-7	2
β-strand	8-12	5	1
β-strand	16-21	6	1
β-strand	22	1	2
β-strand	24	1	2
β-strand	29-32	4	1
β-strand	35-38	4	3
α-helix	39	1
β-strand	53-54	2	3
α-helix	55-60	6
β-strand	65-68	4	3
β-strand	71-72	2	4
β-strand	75-76	2	4
α-helix	79-87	9
α-helix	88-93	6
α-helix	98-100	3
β-strand	103-107	5	1
α-helix	113-125	13
β-strand	131-136	6	1
α-helix	137-144	8
β-strand	150-155	6	5
β-strand	160-166	7	5
β-strand	169-170	2	5
α-helix	172-174	3
β-strand	176-178	3	5
α-helix	182-194	13
α-helix	203-216	14
α-helix	223-232	10
β-strand	238-241	4	6
β-strand	247-250	4	6
α-helix	253-256	4
α-helix	259-262	4
α-helix	264-267	4
α-helix	274-283	10
α-helix	290-295	6
β-strand	297-300	4	5
α-helix	302-304	3
α-helix	309-320	12
β-strand	329-330	2	5
α-helix	338-346	9
α-helix	350-354	5
β-strand	357-358	2	1
α-helix	359-365	7
α-helix	369-373	5

Chain G: 13 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	6-10	5
β-strand	16-23	8	7
β-strand	26-29	4	7
α-helix	30-31	2
α-helix	32-34	3
β-strand	37-39	3	8
β-strand	43-51	9	7
β-strand	57-65	9	7
α-helix	71-87	17
β-strand	92-98	7	7
α-helix	104-107	4
β-strand	115-117	3	8
α-helix	121-123	3
α-helix	1183-1191	9
β-strand	1199-1201	3	9
α-helix	1210-1220	11
β-strand	1228-1365	3	9
α-helix	1373-1385	13
β-strand	1391-1393	3	9
α-helix	1401-1410	10
α-helix	1411-1413	3
β-strand	1419-1421	3	9
α-helix	1431-1440	10
β-strand	1449-1451	3	9
α-helix	1457-1482	26

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Actin, alpha skeletal muscle	A	protein	377	Oryctolagus cuniculus	P68135 (AlphaFold model)
Gelsolin, Tropomodulin-1, Leiomodin-1 chimera	G	protein	326	Homo sapiens	P06396 (AlphaFold model), P28289 (AlphaFold model), P29536 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>4Z94_1 Actin, alpha skeletal muscle (chains A)
MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEA
QSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREK
MTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRL
DLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEK
SYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNV
MSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWIT
KQEYDEAGPSIVHRKCF

Sequence of entity 2 (G), FASTA

>4Z94_2 Gelsolin, Tropomodulin-1, Leiomodin-1 chimera (chains G)
MVVEHPEFLKAGKEPGLQIWRVEKFDLVPVPTNLYGDFFTGDAYVILKTVQLRNGNLQYD
LHYWLGNECSQDESGAAAIFTVQLDDYLNGRAVQHREVQGFESATFLGYFKSGLKYKKGG
VASGFGGSGGSGGSGLNSVIKPTQYKPVPDEEPNSTDVEETLERIKNNDPKLEEVNLNNI
RNIPIPTLKAYAEALKENSYVKKFALANTRADDHVAFAIAIMLKANKTITSLNLDSNHIT
GKGILAIFRALLQNNTLTELRFHNQRHICGGKTEMEIAKLLKENTTLLKLGYHFELAGPR
MTVTNLLSRNMDKQRQKRLQEQRQAQ

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	3
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	1

Primary citation

How Leiomodin and Tropomodulin use a common fold for different actin assembly functions. Boczkowska, M., Rebowski, G., Kremneva, E. et al. Nat Commun (2015) 6:8314-8314. DOI 10.1038/ncomms9314 · PubMed

Other PDB entries of the same protein (UniProt P68135 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4B1Y 1.29 Å, Structure of the Phactr1 RPEL-3 bound to G-actin
2FXU 1.35 Å, X-ray Structure of Bistramide A- Actin Complex at 1.35 A resolution.
4K41 1.4 Å, Crystal structure of actin in complex with marine macrolide kabiramide C
1QZ5 1.45 Å, Structure of rabbit actin in complex with kabiramide C
1WUA 1.45 Å, The structure of Aplyronine A-actin complex
2Q0U 1.45 Å, Structure of Pectenotoxin-2 and Latrunculin B Bound to Actin
2V52 1.45 Å, Structure of MAL-RPEL2 complexed to G-actin
3MN5 1.5 Å, Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
2PBD 1.5 Å, Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP*
5ZZA 1.53 Å, OdinProfilin/Rabbit Actin Complex
1J6Z 1.54 Å, Uncomplexed actin
1QZ6 1.6 Å, Structure of rabbit actin in complex with jaspisamide A

Browse structure collections

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