5CBO: Mbp3-16,Immunoglobulin G-binding protein A

Fusion protein of mbp3-16 and B4 domain of protein A from staphylococcal aureus. Determined by X-ray diffraction at 2.8 Å resolution. Released 30 Mar 2016.

Method: X-ray diffraction
Resolution: 2.8 Å
Organisms: synthetic construct, Staphylococcus aureus
Chains: 12
Atoms: 16,150
Mol. weight: 229.12 kDa
Released: 30 Mar 2016

Explore 5CBO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5CBO contains 132 α-helices and 0 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A, E, I, J, K and L: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	14-24	11
α-helix	27-35	9
α-helix	50-56	7
α-helix	60-68	9
α-helix	83-88	6
α-helix	93-101	9
α-helix	116-122	7
α-helix	126-1228	21
α-helix	1235-1247	13
α-helix	1249-1251	3
α-helix	1252-1263	12

Chains B, C, D, F and H: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	14-24	11
α-helix	27-35	9
α-helix	50-56	7
α-helix	60-68	9
α-helix	83-88	6
α-helix	93-101	9
α-helix	116-122	7
α-helix	126-1228	21
α-helix	1235-1247	13
α-helix	1249-1251	3
α-helix	1252-1265	14

Chain G: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	14-24	11
α-helix	27-35	9
α-helix	50-56	7
α-helix	60-68	9
α-helix	83-88	6
α-helix	93-101	9
α-helix	116-123	8
α-helix	126-1228	21
α-helix	1235-1247	13
α-helix	1249-1251	3
α-helix	1252-1265	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
mbp3-16,Immunoglobulin G-binding protein A	A, B, C, D, E, F, G, H, I, J, K, L	protein	176	synthetic construct, Staphylococcus aureus	P38507 (AlphaFold model)

Sequence of entity 1 (A, B, C, D, E, F, G, H, I, J, K, L), FASTA

>5CBO_1 mbp3-16,Immunoglobulin G-binding protein A (chains A, B, C, D, E, F, G, H, I, J, K, L)
SDLGKKLLEAAHAGQDDEVRILMANGADVNAMDNFGVTPLHLAAYWGHFEIVEVLLKYGA
DVNASDATGDTPLHLAAKWGYLGIVEVLLKYGADVNAQDKFGKTAFDISIDNGNEDLAEI
LCKNKAQQAAFYCILHMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPK

Primary citation

Connecting two proteins using a fusion alpha helix stabilized by a chemical cross linker. Jeong, W.H., Lee, H., Song, D.H. et al. Nat Commun (2016) 7:11031-11031. DOI 10.1038/ncomms11031 · PubMed

Other PDB entries of the same protein (UniProt P38507 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4NPD 0.9 Å, High-resolution structure of C domain of staphylococcal protein A at cryogenic temperature
4NPE 1.42 Å, High-resolution structure of C domain of staphylococcal protein A at room temperature
4NPF 1.49 Å, High-resolution structure of two tandem B domains of staphylococcal protein A connected…
8CPL 1.6 Å, YZw2 a scaffold for cryo-EM of small proteins of interest
4ZMD 1.87 Å, C domain of staphylococcal protein A mutant - Q9W
4ZNC 2.28 Å, Fc fragment of human IgG in complex with the C domain of staphylococcal protein A mutant…
1LP1 2.3 Å, Protein Z in complex with an in vitro selected affibody
5CBN 2.3 Å, Fusion protein of mbp3-16 and B4 domain of protein A from staphylococcal aureus with…
4WWI 2.31 Å, Crystal structure of the C domain of staphylococcal protein A in complex with the Fc…
5H7D 2.57 Å, Crystal structure of the YgjG-protein A-Zpa963-calmodulin complex
5EWX 2.6 Å, Fusion protein of T4 lysozyme and B4 domain of protein A from staphylococcal aureus with…
5H76 2.6 Å, Crystal structure of the DARPin-Protein A fusion protein

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