5K5S: Extracellular calcium-sensing receptor

Crystal structure of the active form of human calcium-sensing receptor extracellular domain. Determined by X-ray diffraction at 2.6 Å resolution. Released 3 Aug 2016.

Method: X-ray diffraction
Resolution: 2.6 Å
Organism: Homo sapiens
Chains: 2
Atoms: 8,913
Mol. weight: 140.7 kDa
Ligands: TRP, PO4, CA, NAG
Released: 3 Aug 2016

Explore 5K5S in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5K5S contains 51 α-helices and 64 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 26 helices, 29 β-strands

Element	Residues	Length	Sheet
β-strand	25-28	4	1
β-strand	32-38	7	1
β-strand	41	1	2
β-strand	44	1	3
α-helix	54-59	6
β-strand	60	1	3
β-strand	63	1	2
α-helix	65-83	19
β-strand	93-99	7	1
α-helix	104-114	11
β-strand	138-142	5	1
α-helix	147-159	13
β-strand	164-166	3	1
α-helix	172-175	4
β-strand	183-185	3	1
α-helix	191-203	13
β-strand	208-214	7	4
α-helix	219-232	14
β-strand	236-243	8	4
α-helix	249-260	12
β-strand	266-270	5	4
α-helix	273-285	13
β-strand	292-295	4	4
α-helix	308-310	3
α-helix	311-314	4
β-strand	318-322	5	4
α-helix	323-324	2
β-strand	325	1	5
α-helix	330-335	6
β-strand	345	1	1
α-helix	348-355	8
β-strand	414	1	5
α-helix	416-435	20
α-helix	445-447	3
α-helix	448-451	4
α-helix	452-454	3
α-helix	457-465	9
β-strand	468-470	3	6
β-strand	476-478	3	6
β-strand	489-496	8	4
β-strand	503-511	9	4
α-helix	516	1
β-strand	521-523	3	4
α-helix	525-527	3
β-strand	530	1	7
β-strand	534	1	7
α-helix	537-539	3
α-helix	544-547	4
β-strand	550-554	5	8
β-strand	563-567	5	8
α-helix	568-569	2
β-strand	572-573	2	9
α-helix	582	1
β-strand	583-584	2	9
α-helix	585-586	2

Chain B: 25 helices, 35 β-strands

Element	Residues	Length	Sheet
β-strand	25-28	4	10
β-strand	32-38	7	10
β-strand	41	1	11
β-strand	44	1	12
α-helix	54-59	6
β-strand	60	1	12
β-strand	63	1	11
α-helix	65-83	19
β-strand	93-99	7	10
α-helix	104-114	11
α-helix	119-121	3
β-strand	138-142	5	10
α-helix	147-159	13
β-strand	164-166	3	10
α-helix	172-175	4
β-strand	183-185	3	10
α-helix	191-203	13
β-strand	208-214	7	13
α-helix	219-232	14
β-strand	236-243	8	13
α-helix	249-260	12
β-strand	266-270	5	13
α-helix	273-285	13
β-strand	292-295	4	13
α-helix	308-310	3
α-helix	311-314	4
β-strand	318-322	5	13
β-strand	325	1	14
α-helix	330-335	6
β-strand	345	1	10
α-helix	348-355	8
β-strand	359	1	15
α-helix	393	1
β-strand	394	1	15
α-helix	395	1
β-strand	414	1	14
α-helix	416-435	20
β-strand	444	1	16
β-strand	448	1	16
α-helix	457-465	9
β-strand	468-470	3	17
β-strand	476-478	3	17
β-strand	489-496	8	13
β-strand	503-511	9	13
α-helix	516	1
β-strand	521-523	3	13
α-helix	525-527	3
β-strand	530	1	18
β-strand	534	1	18
α-helix	537-539	3
α-helix	544-547	4
β-strand	550-554	5	19
β-strand	563-567	5	19
α-helix	568-569	2
β-strand	572-573	2	20
α-helix	582	1
β-strand	583-584	2	20
α-helix	585-586	2
β-strand	589-591	3	21
β-strand	598-600	3	21

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Extracellular calcium-sensing receptor	A, B	protein	615	Homo sapiens	P41180 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>5K5S_1 Extracellular calcium-sensing receptor (chains A, B)
MAFYSCCWVLLALTWHTSAYGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVEC
IRYNFRGFRWLQAMIFAIEEINSSPALLPNLTLGYRIFDTCNTVSKALEATLSFVAQNKI
DSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQF
KSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKFREEAEERDICIDFS
ELISQYSDEEEIQHVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGKIWLASEAWA
SSSLIAMPQYFHVVGGTIGFALKAGQIPGFREFLKKVHPRKSVHNGFAKEFWEETFNCHL
QEGAKGPLPVDTFLRGHEESGDRFSQSSTAFRPLCTGDENINSVETPYIDYTHLRISYNV
YLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHLQFTNNMGEQVTFD
ECGDLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEEKILWSGFSREVPFS
NCSRDCLAGTRKGIIEGEPTCCFECVECPDGEYSDETDASACNKCPDDFWSNENHTSCIA
KEIEFLSDYKDDDDK

Ligands and cofactors

ID	Name	Formula	Copies
TRP	Tryptophan	C11 H12 N2 O2	2
PO4	Phosphate ion	O4 P	4
CA	Calcium ion	Ca	8
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	5

Primary citation

Structural mechanism of ligand activation in human calcium-sensing receptor. Geng, Y., Mosyak, L., Kurinov, I. et al. Elife (2016) 5. DOI 10.7554/eLife.13662 · PubMed

Other PDB entries of the same protein (UniProt P41180 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5FBK 2.1 Å, Crystal structure of the extracellular domain of human calcium sensing receptor
7M3G 2.5 Å, Asymmetric Activation of the Calcium Sensing Receptor Homodimer
5FBH 2.7 Å, Crystal structure of the extracellular domain of human calcium sensing receptor with…
7SIL 2.7 Å, Structure of positive allosteric modulator-bound active human calcium-sensing receptor
7SIM 2.7 Å, Structure of positive allosteric modulator-free active human calcium-sensing receptor
8WPG 2.7 Å, Human calcium-sensing receptor bound with cinacalcet in detergent
7M3F 2.8 Å, Asymmetric Activation of the Calcium Sensing Receptor Homodimer
8SZF 2.8 Å, Cryo-EM structure of cinacalcet-bound active-state human calcium-sensing receptor CaSR…
9C1P 2.8 Å, Structure of Calcium-Sensing Receptor in complex with positive allosteric modulator '6218
9C2F 2.8 Å, Structure of Calcium-Sensing Receptor in complex with positive allosteric modulator '54149
7E6T 3.0 Å, Structural insights into the activation of human calcium-sensing receptor
5K5T 3.1 Å, Crystal structure of the inactive form of human calcium-sensing receptor extracellular…

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