5OSA: GLIC-GABAAR alpha1 chimera crystallized at pH4.6

GLIC-GABAAR alpha1 chimera crystallized at pH4.6. Determined by X-ray diffraction at 2.75 Å resolution. Released 11 Oct 2017.

Method: X-ray diffraction
Resolution: 2.75 Å
Organisms: Gloeobacter violaceus (strain PCC 7421), Mus musculus
Chains: 5
Atoms: 12,802
Mol. weight: 197.74 kDa
Ligands: D12, Y01
Released: 11 Oct 2017

Explore 5OSA in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5OSA contains 61 α-helices and 61 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	5-8	4
α-helix	13-14	2
β-strand	15-30	16	1
β-strand	35-47	13	1
α-helix	49-51	3
β-strand	64	1	1
β-strand	75-77	3	2
β-strand	80	1	1
α-helix	83-84	2
β-strand	85-93	9	1
β-strand	98-110	13	1
β-strand	122-132	11	2
β-strand	139-143	5	1
α-helix	145-147	3
β-strand	149-150	2	1
β-strand	159-166	8	2
β-strand	171-175	5	2
β-strand	178-191	14	2
α-helix	223-225	3
α-helix	226-230	5
α-helix	231-242	12
α-helix	243-245	3
α-helix	251-273	23
α-helix	284-308	25
α-helix	314-414	30

Chains B and D: 12 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	5-8	4
α-helix	14	1
β-strand	15-30	16	3
β-strand	35-47	13	3
α-helix	49-51	3
β-strand	64	1	3
β-strand	75-77	3	4
β-strand	80	1	3
α-helix	83-84	2
β-strand	85-93	9	3
β-strand	98-110	13	3
β-strand	122-132	11	4
β-strand	139-143	5	3
α-helix	145-147	3
β-strand	149-150	2	3
β-strand	159-175	17	4
β-strand	178-191	14	4
α-helix	223-225	3
α-helix	226-230	5
α-helix	231-242	12
α-helix	243-245	3
α-helix	251-273	23
α-helix	284-308	25
α-helix	314-414	30

Chain C: 13 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	5-8	4
α-helix	14	1
β-strand	15-30	16	5
β-strand	35-47	13	5
α-helix	49-51	3
β-strand	75-77	3	6
β-strand	80	1	5
α-helix	83-84	2
β-strand	85-93	9	5
β-strand	98-110	13	5
β-strand	122-132	11	6
α-helix	133-134	2
β-strand	139-143	5	5
α-helix	145-147	3
β-strand	149-150	2	5
β-strand	159-166	8	6
β-strand	171-175	5	6
β-strand	178-191	14	6
α-helix	223-225	3
α-helix	226-230	5
α-helix	231-242	12
α-helix	243-245	3
α-helix	251-273	23
α-helix	284-308	25
α-helix	314-413	29

Chain E: 12 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	5-8	4
α-helix	13-14	2
β-strand	15-30	16	9
β-strand	35-47	13	9
α-helix	49-51	3
β-strand	64	1	9
β-strand	75-77	3	10
β-strand	80	1	9
α-helix	83-84	2
β-strand	85-93	9	9
β-strand	98-110	13	9
β-strand	122-132	11	10
β-strand	139-143	5	9
α-helix	145-147	3
β-strand	149-150	2	9
β-strand	159-175	17	10
β-strand	178-191	14	10
α-helix	223-225	3
α-helix	226-230	5
α-helix	231-242	12
α-helix	243-245	3
α-helix	251-273	23
α-helix	284-308	25
α-helix	314-414	30

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Proton-gated ion channel,Gamma-aminobutyric acid receptor subunit alpha-1,Gamma-aminobutyric acid…	A, B, C, D, E	protein	336	Gloeobacter violaceus (strain PCC 7421), Mus musculus	P62812 (AlphaFold model), Q7NDN8 (AlphaFold model)

Sequence of entity 1 (A, B, C, D, E), FASTA

>5OSA_1 Proton-gated ion channel,Gamma-aminobutyric acid receptor subunit alpha-1,Gamma-aminobutyric acid receptor subunit alpha-1 (chains A, B, C, D, E)
QDMVSPPPPIADEPLTVNTGIYLIECYSLDDKAETFKVNAFLSLSWKDRRLAFDPVRSGV
RVKTYEPEAIWIPEIRFVNVENARDADVVDISVSPDGTVQYLERFSARVLSPLDFRRYPF
DSQTLHIYLIVRSVDTRNIVLAVDLEKVGKNDDVFLTGWDIESFTAVVKPANFALEDRLE
SKLDYQLRISRQYGYFVIQTYLPCIMTVILSQVSFWLNRESVPARTVFVVTTVLTMTTLS
ISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKSQPARAAKIDRLSRIAFP
LLFGIFNLVYWATYLNREPQLKAPTPHQHHHHHHHH

Ligands and cofactors

ID	Name	Formula	Copies
D12	Dodecane	C12 H26	8
Y01	Cholesterol hemisuccinate	C31 H50 O4	5

Water and common crystallization additives (ACT, CL) are not listed.

Primary citation

Crystal structures of a GABAA-receptor chimera reveal new endogenous neurosteroid-binding sites. Laverty, D., Thomas, P., Field, M. et al. Nat Struct Mol Biol (2017) 24:977-985. DOI 10.1038/nsmb.3477 · PubMed

Other PDB entries of the same protein (UniProt P62812 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8FOI 2.5 Å, Native GABA-A receptor from the mouse brain, alpha1-beta2-gamma2 subtype, in complex…
8G4X 2.56 Å, Native GABA-A receptor from the mouse brain, meta-alpha1-alpha3-beta2-gamma2 subtype, in…
8G5F 2.64 Å, Native GABA-A receptor from the mouse brain, ortho-alpha1-alpha3-beta2-gamma2 subtype,…
8G4N 2.67 Å, Native GABA-A receptor from the mouse brain, alpha1-beta2-gamma2 subtype, in complex…
8G5H 2.89 Å, Native GABA-A receptor from the mouse brain, ortho-alpha1-alpha3-beta2-gamma2 subtype,…
8G5G 2.94 Å, Native GABA-A receptor from the mouse brain, meta-alpha1-alpha3-beta2-gamma2 subtype, in…
8G4O 3.06 Å, Native GABA-A receptor from the mouse brain, alpha1-beta2-gamma2 subtype, in complex…
5OSC 3.1 Å, GLIC-GABAAR alpha1 chimera crystallized in complex with pregnenolone sulfate at pH 4.5
5OSB 3.8 Å, GLIC-GABAAR alpha1 chimera crystallized in complex with THDOC at pH4.5

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