5WIE: Voltage-gated potassium channel subunit beta-2

Crystal structure of a Kv1.2-2.1 chimera K+ channel V406W mutant in an inactivated state. Determined by X-ray diffraction at 3.3 Å resolution. Released 30 Aug 2017.

Method

X-ray diffraction

Resolution

3.3 Å

Organisms

Rattus norvegicus, Mus musculus

Chains

4

Atoms

10,955

Mol. weight

201.91 kDa

Ligands

NAP, PGW

Released

30 Aug 2017

Explore 5WIE in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5WIE contains 84 α-helices and 38 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 23 helices, 16 β-strands

Molecules and chains

>5WIE_1 Voltage-gated potassium channel subunit beta-2 (chains A, G)
MLQFYRNLGKSGLRVSCLGLGTWVTFGGQITDEMAEHLMTLAYDNGINLFDTAEVYAAGK
AEVVLGNIIKKKGWRRSSLVITTKIFWGGKAETERGLSRKHIIEGLKASLERLQLEYVDV
VFANRPDPNTPMEETVRAMTHVINQGMAMYWGTSRWSSMEIMEAYSVARQFNLIPPICEQ
AEYHMFQREKVEVQLPELFHKIGVGAMTWSPLACGIVSGKYDSGIPPYSRASLKGYQWLK
DKILSEEGRRQQAKLKELQAIAERLGCTLPQLAIAWCLRNEGVSSVLLGASNAEQLMENI
GAIQVLPKLSSSIVHEIDSILGNKPYSKKDYRS

>5WIE_2 Potassium voltage-gated channel subfamily A member 2,Potassium voltage-gated channel subfamily A member 2 (chains B, H)
MSAWSHPQFEKGGGSGGGSGGSAWSHPQFEKLVPRGSMTVATGDPVDEAAAHPGHPQDTY
DPEADHESSERVVINISGLRFETQLKTLAQFPETLLGDPKKRMRYFDPLRNEYFFDRNRP
SFDAILYYYQSGGRLRRPVNVPLDIFSEEIRFYELGEEAMEMFREDEGYIKEEERPLPEN
EFQRQVWLLFEYPESSGPARIIAIVSVMVILISIVSFCLETLPIFRDENEDMHGGGVTFH
TYSQSTIGYQQSTSFTDPFFIVETLCIIWFSFEFLVRFFACPSKAGFFTNIMNIIDIVAI
IPYYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRIFKLSRHSKGLQILGQTLKASMRE
LGLLIFFLFIGVILFSSAVYFAEADERDSQFPSIPDAFWWAVVSMTTVGYGDMVPTTIGG
KIVGSLCAIAGVLTIALPVPVIWSNFNYFYHRETEGEEQAQYLQVTSSPKIPSSPDLKKS
RSASTISKSDYMEIQEGVNNSNEDFREENLKTANSTLANTNYVNITKMLTDV

Ligands and cofactors

Water and common crystallization additives (K) are not listed.

Primary citation

Crystal structure of an inactivated mutant mammalian voltage-gated K(+) channel. Pau, V., Zhou, Y., Ramu, Y. et al. Nat Struct Mol Biol (2017) 24:857-865. DOI 10.1038/nsmb.3457 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P62483 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 3EAU 1.82 Å, Voltage-dependent K+ channel beta subunit in complex with cortisone
• 3EB3 2.0 Å, Voltage-dependent K+ channel beta subunit (W121A) in complex with cortisone
• 3EB4 2.0 Å, Voltage-dependent K+ channel beta subunit (I211R) in complex with cortisone
• 1EXB 2.1 Å, Structure of the cytoplasmic beta subunit-T1 assembly of voltage-dependent K channels
• 2R9R 2.4 Å, Shaker family voltage dependent potassium channel (kv1.2-kv2.1 paddle chimera channel)…
• 4JTA 2.5 Å, Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Charybdotoxin
• 4JTD 2.54 Å, Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Lys27Met mutant of…
• 4JTC 2.56 Å, Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Charybdotoxin in Cs+
• 1QRQ 2.8 Å, Structure of a voltage-dependent K+ channel beta subunit
• 2A79 2.9 Å, Mammalian Shaker Kv1.2 potassium channel- beta subunit complex
• 3LNM 2.9 Å, F233W mutant of the Kv2.1 paddle-Kv1.2 chimera channel
• 3LUT 2.9 Å, A Structural Model for the Full-length Shaker Potassium Channel Kv1.2

Browse structure collections

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