6J8Y: Human RAD9-HUS1-RAD1-RHINO complex

Crystal structure of the human RAD9-HUS1-RAD1-RHINO complex. Determined by X-ray diffraction at 2.4 Å resolution. Released 4 Dec 2019.

Method: X-ray diffraction
Resolution: 2.4 Å
Organism: Homo sapiens
Chains: 4
Atoms: 6,267
Mol. weight: 96.37 kDa
Released: 4 Dec 2019

Explore 6J8Y in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6J8Y contains 26 α-helices and 56 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	2-6	5	1
α-helix	9-20	12
β-strand	26-32	7	2
β-strand	35-41	7	2
β-strand	47-53	7	2
α-helix	55-57	3
β-strand	60-62	3	1
β-strand	73-76	4	2
α-helix	77-83	7
α-helix	87-92	6
β-strand	94-100	7	1
β-strand	107-114	8	1
α-helix	115-117	3
β-strand	119-125	7	1
β-strand	127-128	2	2
α-helix	138-140	3
β-strand	143-148	6	2
α-helix	149-156	8
β-strand	165-171	7	3
β-strand	175-180	6	3
α-helix	191-193	3
β-strand	194-199	6	3
α-helix	201-203	3
β-strand	206-208	3	2
β-strand	214-218	5	3
α-helix	219-230	12
β-strand	235-240	6	2
β-strand	247-252	6	2
β-strand	256-262	7	2

Chain B: 9 helices, 18 β-strands

Element	Residues	Length	Sheet
β-strand	2-7	6	3
α-helix	10-26	17
β-strand	29-34	6	4
β-strand	38-44	7	4
β-strand	53-59	7	4
α-helix	60-62	3
β-strand	66-70	5	3
β-strand	79-84	6	4
α-helix	85-95	11
β-strand	100-107	8	3
β-strand	111-119	9	3
β-strand	127-134	8	3
β-strand	136-137	2	4
α-helix	138-139	2
α-helix	140-146	7
α-helix	148-150	3
α-helix	152-154	3
β-strand	156-159	4	4
α-helix	163-174	12
β-strand	179-184	6	5
β-strand	190-195	6	5
β-strand	199-205	7	5
β-strand	228-233	6	5
α-helix	234-242	9
β-strand	250-256	7	4
β-strand	260-266	7	4
β-strand	270-277	8	4

Chain C: 7 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	17-21	5	5
α-helix	25-32	8
β-strand	39-45	7	6
β-strand	48-55	8	6
β-strand	59-66	8	6
β-strand	72-75	4	5
β-strand	80-85	6	6
α-helix	86-93	8
β-strand	107-112	6	5
α-helix	118	1
β-strand	119-125	7	5
β-strand	128-134	7	5
β-strand	137	1	6
α-helix	149-151	3
β-strand	152-159	8	6
α-helix	161-167	7
β-strand	176-181	6	1
β-strand	188-194	7	1
β-strand	197-203	7	1
β-strand	210-215	6	6
β-strand	219-224	6	1
α-helix	225-228	4
α-helix	231-237	7
β-strand	238-246	9	6
β-strand	251-258	8	6
β-strand	264-271	8	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Cell cycle checkpoint control protein RAD9A	A	protein	270	Homo sapiens	Q99638 (AlphaFold model)
Checkpoint protein HUS1	B	protein	286	Homo sapiens	O60921 (AlphaFold model)
Cell cycle checkpoint protein RAD1	C	protein	282	Homo sapiens	O60671 (AlphaFold model)
RAD9, HUS1, RAD1-interacting nuclear orphan protein 1	D	protein	20	Homo sapiens	Q9BSD3 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>6J8Y_1 Cell cycle checkpoint control protein RAD9A (chains A)
MKCLVTGGNVKVLGKAVHSLSRIGDELYLEPLEDGLSLRTVNSSRSAYACFLFAPLFFQQ
YQAATPGQDLLRCKILMKSFLSVFRSLAMLEKTVEKCCISLNGRSSRLVVQLHCKFGVRK
THNLSFQDCESLQAVFDPASCPHMLRAPARVLGEAVLPFSPALAEVTLGIGRGRRVILRS
YHEEEADSTAKAMVTEMCLGEEDFQQLQAQEGVAITFCLKEFRGLLSFAESANLNLSIHF
DAPGRPAIFTIKDSLLDGHFVLATLSDTDS

Sequence of entity 2 (B), FASTA

>6J8Y_2 Checkpoint protein HUS1 (chains B)
MHHHHHHKFRAKIVDGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSM
WCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLT
VSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMK
NISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDI
RKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS

Sequence of entity 3 (C), FASTA

>6J8Y_3 Cell cycle checkpoint protein RAD1 (chains C)
MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQ
ANAFIQAGIFQEFKVQEESVTFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLM
LFLEEGGVVTVCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQIT
MSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKPSTKALVLSCK
VSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPESES

Sequence of entity 4 (D), FASTA

>6J8Y_4 RAD9, HUS1, RAD1-interacting nuclear orphan protein 1 (chains D)
SKPIDHSTITSWVSPDFDTA

Primary citation

Structure of the RAD9-RAD1-HUS1 checkpoint clamp bound to RHINO sheds light on the other side of the DNA clamp. Hara, K., Iida, N., Tamafune, R. et al. J Biol Chem (2020) 295:899-904. DOI 10.1074/jbc.AC119.011816 · PubMed

Other PDB entries of the same protein (UniProt Q99638 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8JZY 1.5 Å, RPA70N-RAD9 fusion
8GNN 2.12 Å, Crystal structure of the human RAD9-RAD1-HUS1-RAD17 complex
6HM5 2.33 Å, Crystal structure of TOPBP1 BRCT0,1,2 in complex with a RAD9 phosphopeptide
3A1J 2.5 Å, Crystal structure of the human Rad9-Hus1-Rad1 complex
8WU8 2.81 Å, Crystal structure of the human RAD9-RAD1(F64A/M256A/F266A)-HUS1-RHINO(88-99) complex
3G65 2.9 Å, Crystal Structure of the Human Rad9-Rad1-Hus1 DNA Damage Checkpoint Complex
3GGR 3.2 Å, Crystal Structure of the Human Rad9-Hus1-Rad1 complex
7Z6H 3.59 Å, Structure of DNA-bound human RAD17-RFC clamp loader and 9-1-1 checkpoint clamp

Browse structure collections

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