6K07: REV7(R124A)

Crystal structure of REV7(R124A) in complex with a Shieldin3 fragment. Determined by X-ray diffraction at 2.24 Å resolution. Released 11 Dec 2019.

Method: X-ray diffraction
Resolution: 2.24 Å
Organism: Homo sapiens
Chains: 2
Atoms: 1,737
Mol. weight: 28.65 kDa
Released: 11 Dec 2019

Explore 6K07 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6K07 contains 12 α-helices and 9 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	11-33	23
α-helix	39-41	3
β-strand	42-47	6	1
β-strand	50-55	6	1
α-helix	58-76	19
β-strand	80-88	9	2
β-strand	94-103	10	2
α-helix	115-132	18
α-helix	133-135	3
α-helix	138-141	4
β-strand	145-152	8	2
α-helix	153	1
α-helix	159-162	4
β-strand	171-173	3	2
α-helix	174-175	2
α-helix	184	1
β-strand	185-193	9	2
β-strand	198-205	8	2

Chain B: 2 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	51-53	3	2
α-helix	56-59	4
α-helix	62-71	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Mitotic spindle assembly checkpoint protein MAD2B	A	protein	219	Homo sapiens	Q9UI95 (AlphaFold model)
Shieldin complex subunit 3	B	protein	30	Homo sapiens	Q6ZNX1 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>6K07_1 Mitotic spindle assembly checkpoint protein MAD2B (chains A)
MGSSHHHHHHSQDPQDLNFGQVVADVLCEFLEVAVHLILYVREVYPVGIFQKRKKYNVPV
QMSCHPELNQYIQDTLHCVKPLLEKNDVEKVVVVILDKEHRPVEKFVFEITQPPLLSISS
DSLLSHVEQLLAAFILKISVCDAVLDHNPPGCTFTVLVHTREAATRNMEKIQVIKDFPWI
LADEQDVHMHDPRLIPLKTMTSDILKMQLYVEERAHKGS

Sequence of entity 2 (B), FASTA

>6K07_2 Shieldin complex subunit 3 (chains B)
MGSKLPLRPKRSPPVISEEAAEDVKQYLTI

Primary citation

Structural basis for shieldin complex subunit 3-mediated recruitment of the checkpoint protein REV7 during DNA double-strand break repair. Dai, Y., Zhang, F., Wang, L. et al. J Biol Chem (2020) 295:250-262. DOI 10.1074/jbc.RA119.011464 · PubMed

Other PDB entries of the same protein (UniProt Q9UI95 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6BCD 1.43 Å, Crystal structure of Rev7-K44A/R124A/A135D in complex with Rev3-RBM2 (residues 1988-2014)
6BC8 1.68 Å, Crystal structure of Rev7-R124A/Rev3-RBM2 (residues 1988-2014) complex
6M7B 1.77 Å, Structure of REV7-R124A complexed with SHLD3(37-73)
3ABD 1.9 Å, Structure of human REV7 in complex with a human REV3 fragment in a monoclinic crystal
4EXT 1.9 Å, Structure of polymerase-interacting domain of human Rev1 in complex with translesional…
6M7A 1.9 Å, Structure of REV7-R124A complexed with SHLD3(28-73)
6VE5 2.0 Å, X-ray structure of human REV7 in complex with Shieldin3 (residues 41-74)
6NIF 2.0 Å, crystal structure of human REV7-RAN complex
5XPT 2.1 Å, Crystal structure of MAD2L2/REV7 in complex with a CAMP fragment in a tetragonal crystal
6WS0 2.24 Å, Rational drug design of phenazopyridine derivatives as novel inhibitors of Rev1-CT
5XPU 2.3 Å, Crystal structure of MAD2L2/REV7 in complex with a CAMP fragment in a monoclinic crystal
6K08 2.31 Å, Crystal structure of REV7(R124A/A135D) in complex with a Shieldin3 fragment

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