6T1W: E. coli BamA

Structure of E. coli BamA in complex with lipoprotein RcsF. Determined by X-ray diffraction at 3.79 Å resolution. Released 13 May 2020.

Method

X-ray diffraction

Resolution

3.79 Å

Organism

Escherichia coli K-12

Chains

4

Atoms

10,838

Mol. weight

209.65 kDa

Released

13 May 2020

Explore 6T1W in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6T1W contains 38 α-helices and 105 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 23 helices, 57 β-strands

Molecules and chains

>6T1W_1 Outer membrane protein assembly factor BamA (chains A, B)
MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALLSMPVRTGDTVNDEDIS
NTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGNKSVKDDMLKQNLEASGVR
VGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPRNRVDLKLVFQEGVSAEIQQI
NIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQKLAGDLETLRSYYLDRGYARFNI
DSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGNLAGHSAEIEQLTKIEPGELYNGTK
VTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKTVKLRVNVDAGNRFYVRKIRFEGNDTS
KDAVLRREMRQMEGAWLGSDLVDQGKERLNRLGFFETVDTDTQRVPGSPDQVDVVYKVKE
RNTGSFNFGIGYGTESGVSFQAGVQQDNWLGTGYAVGINGTKNDYQTYAELSVTNPYFTV
DGVSLGGRLFYNDFQADDADLSDYTNKSYGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQ
PQVAMWRYLYSMGEHPSTSDQDNSFKTDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVT
IPGSDNEYYKVTLDTATYVPIDDDHKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTV
RGFQSNTIGPKAVYFPHQASNYDPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFIT
PTPFISDKYANSVRTSFFWDMGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPL
GPLVFSYAQPFKKYDGDKAEQFQFNIGKTW

>6T1W_2 Outer membrane lipoprotein RcsF (chains C, D)
MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAPRATPVRIYTNAEELVG
KPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKANAVLLHSCEVTSGTPGCYR
QAVCIGSALNITAK

Primary citation

Structural insight into the formation of lipoprotein-beta-barrel complexes. Rodriguez-Alonso, R., Letoquart, J., Nguyen, V.S. et al. Nat Chem Biol (2020) 16:1019-1025. DOI 10.1038/s41589-020-0575-0 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P0A940 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 3Q6B 1.5 Å, The high-resolution and new form crystal structure of BamA POTRA4-5 from E.coli
• 9CS2 1.78 Å, E. coli BamA beta-barrel bound to cyclic peptide CP3
• 6QGW 1.94 Å, Crystal structure of E.coli BamA beta-barrel in complex with nanobody E6
• 9CS0 1.94 Å, E. coli BamA beta-barrel bound to darobactin and cyclic peptide CP1
• 9CS1 2.15 Å, E. coli BamA beta-barrel bound to darobactin and cyclic peptide CP2
• 4XGA 2.15 Å, Crystal structure of BamB and BamA P3-5 complex from E.coli
• 2QDF 2.2 Å, Structure of N-terminal domain of E. Coli YaeT
• 6QGX 2.2 Å, Crystal structure of E.coli BamA beta-barrel in complex with nanobody F7
• 7NRF 2.2 Å, Crystal structure of E.coli BamA beta-barrel in complex with darobactin (crystal form 2)
• 7NRE 2.3 Å, Crystal structure of E.coli BamA beta-barrel in complex with darobactin (crystal form 1)
• 7P1C 2.5 Å, Crystal structure of E.coli BamA beta-barrel in complex with darobactin B
• 7R1V 2.5 Å, Crystal structure of E.coli BamA beta-barrel in complex with dynobactin A

Browse structure collections

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