6V9I: Cullin5

cryo-EM structure of Cullin5 bound to RING-box protein 2 (Cul5-Rbx2). Determined by electron microscopy at 5.2 Å resolution. Released 29 Apr 2020.

Method

Electron microscopy

Resolution

5.2 Å

Organisms

Streptococcus sp. group G, Homo sapiens, Mus musculus

Chains

2

Atoms

4,066

Mol. weight

113.37 kDa

Ligands

ZN

Released

29 Apr 2020

Explore 6V9I in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6V9I contains 45 α-helices and 23 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain C: 42 helices, 15 β-strands

Molecules and chains

>6V9I_1 Immunoglobulin G-binding protein G,Cullin-5 (chains C)
MGSSHHHHHHSQDPMEYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYD
DATKTFTVTEIPTTENLYFQGEFMATSNLLKNKGSLQFEDKWDFMRPIVLKLLRQESVTK
QQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEW
RKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQD
SAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQ
APSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETI
LAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDS
EKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLK
TQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAH
LTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNN
KLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLM
SNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSL
VAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTE
RMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQI
EWLIEHKYIRRDESDINTFIYMA

>6V9I_2 RING-box protein 2 (chains R)
MADVEDGEEPCVLSSHSGSAGSKSGGDKMFSLKKWNAVAMWSWDVECDTCAICRVQVMDA
CLRCQAENKQEDCVVVWGECNHSFHNCCMSLWVKQNNRCPLCQQDWVVQRIGK

Ligands and cofactors

Primary citation

Structure and dynamics of the ASB9 CUL-RING E3 Ligase. Lumpkin, R.J., Baker, R.W., Leschziner, A.E. et al. Nat Commun (2020) 11:2866-2866. DOI 10.1038/s41467-020-16499-9 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P06654 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 1IGD 1.1 Å, The third IgG-binding domain from streptococcal protein G: an analysis by X-ray…
• 2IGD 1.1 Å, Anisotropic structure of protein G IgG-binding domain III at 1.1 Å resolution
• 3MP9 1.2 Å, Structure of Streptococcal protein G B1 domain at pH 3.0
• 6CNE 1.2 Å, Selenomethionine variant (V29SeM) of protein GB1
• 1PGX 1.66 Å, The 1.66 Å X-ray structure of the B2 immunoglobulin-binding domain of streptococcal…
• 6L9D 1.73 Å, X-ray structure of synthetic GB1 domain with mutations K10(DVA), T11S
• 6L91 1.84 Å, X-ray structure of synthetic GB1 domain with the mutation K10(DVA).
• 6LJI 1.84 Å, X-ray structure of synthetic GB1 domain with mutations K10(DVA), T11V
• 1PGB 1.92 Å, Two crystal structures of the B1 immunoglobulin-binding domain of streptoccocal protein…
• 4KGS 1.95 Å, Backbone Modifications in the Protein GB1 Loops: beta-3-Val21, beta-3-Asp40
• 6L9B 1.95 Å, X-ray structure of synthetic GB1 domain with mutations K10(DVA), T11A
• 1EM7 2.0 Å, Helix variant of the B1 domain from streptococcal protein G

Browse structure collections

About this viewer

MolViewer shows 6V9I directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.