7D2U: Rsu1/PINCH1_LIM45C complex

Crystal structure of Rsu1/PINCH1_LIM45C complex. Determined by X-ray diffraction at 3.15 Å resolution. Released 24 Feb 2021.

Method: X-ray diffraction
Resolution: 3.15 Å
Organism: Homo sapiens
Chains: 2
Atoms: 2,997
Mol. weight: 48.69 kDa
Ligands: ZN, MLI
Released: 24 Feb 2021

Explore 7D2U in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7D2U contains 18 α-helices and 27 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 14 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	11-14	4
β-strand	19-21	3	1
α-helix	30-32	3
α-helix	36-38	3
β-strand	44-46	3	1
α-helix	57-61	5
β-strand	67-69	3	1
α-helix	80-84	5
β-strand	90-92	3	1
α-helix	105-107	3
β-strand	113-115	3	1
α-helix	123-125	3
α-helix	130-132	3
β-strand	138-140	3	1
β-strand	148	1	2
α-helix	151-155	5
β-strand	161-163	3	1
β-strand	171	1	2
α-helix	174-178	5
β-strand	184-186	3	1
α-helix	197-201	5
β-strand	210-212	3	1
α-helix	220-228	9
α-helix	230-236	7
α-helix	240-250	11

Chain B: 4 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	192	1	3
β-strand	199	1	3
β-strand	204-207	4	4
β-strand	210-213	4	4
β-strand	218	1	5
β-strand	225	1	5
α-helix	230-231	2
β-strand	232-234	3	6
β-strand	237-239	3	6
α-helix	241-247	7
β-strand	251	1	7
β-strand	258	1	7
β-strand	263-266	4	8
β-strand	269-272	4	8
β-strand	277	1	9
β-strand	284	1	9
β-strand	291-294	4	10
β-strand	297-300	4	10
α-helix	301-305	5
α-helix	309-317	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Ras suppressor protein 1	A	protein	282	Homo sapiens	Q15404 (AlphaFold model)
LIM and senescent cell antigen-like-containing domain protein 1	B	protein	142	Homo sapiens	P48059 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7D2U_1 Ras suppressor protein 1 (chains A)
GAMGSMSKSLKKLVEESREKNQPEVDMSDRGISNMLDVNGLFTLSHITQLVLSHNKLTMV
PPNIAELKNLEVLNFFNNQIEELPTQISSLQKLKHLNLGMNRLNTLPRGFGSLPALEVLD
LTYNNLSENSLPGNFFYLTTLRALYLSDNDFEILPPDIGKLTKLQILSLRDNDLISLPKE
IGELTQLKELHIQGNRLTVLPPELGNLDLTGQKQVFKAENNPWVTPIADQFQLGVSHVFE
YIRSETYKYLYGRHMQANPEPPKKNNDKSKKISRKPLAAKNR

Sequence of entity 2 (B), FASTA

>7D2U_2 LIM and senescent cell antigen-like-containing domain protein 1 (chains B)
GPGSMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETH
YNQLFGDVCFHCNRVIEGDVVSALNKAWCVNCFACSTCNTKLTLKNKFVEFDMKPVCKKC
YEKFPLELKKRLKKLAETLGRK

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	4
MLI	Malonate ion	C3 H2 O4	1

Water and common crystallization additives (GOL) are not listed.

Primary citation

Complex structures of Rsu1 and PINCH1 reveal a regulatory mechanism of the ILK/PINCH/Parvin complex for F-actin dynamics. Yang, H., Lin, L., Sun, K. et al. Elife (2021) 10. DOI 10.7554/eLife.64395 · PubMed

Other PDB entries of the same protein (UniProt Q15404 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7D2S 1.65 Å, Crystal structure of Rsu1/PINCH1_LIM5C complex
7LT8 1.76 Å, Crystal structure of Ras suppressor-1
7D2T 2.2 Å, Crystal structure of Rsu1/PINCH1_LIM45C complex
7LT9 3.05 Å, Crystal structure of Ras suppressor-1 in complex with PINCH-1 LIM4-5 domains

Browse structure collections

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