7MTS: MGlu2 - Gi Complex

CryoEM Structure of mGlu2 - Gi Complex. Determined by electron microscopy at 3.2 Å resolution. Released 7 Jul 2021.

Method: Electron microscopy
Resolution: 3.2 Å
Organism: Homo sapiens
Chains: 5
Atoms: 16,068
Mol. weight: 274.65 kDa
Ligands: ZQY, NAG, GLU
Released: 7 Jul 2021

Explore 7MTS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7MTS contains 87 α-helices and 87 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 36 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	26-28	3	1
β-strand	32-38	7	1
β-strand	41-43	3	2
β-strand	50-53	4	2
α-helix	59-73	15
β-strand	84-90	7	1
α-helix	95-101	7
α-helix	103-106	4
β-strand	136-141	6	1
α-helix	145-155	11
β-strand	162-164	3	1
α-helix	171-173	3
β-strand	181-183	3	1
α-helix	189-201	13
β-strand	206-212	7	3
α-helix	215-229	15
β-strand	234-241	8	3
α-helix	247-258	12
β-strand	265-269	5	3
α-helix	272-285	14
β-strand	290-293	4	3
α-helix	308-311	4
β-strand	315-319	5	3
α-helix	325-331	7
α-helix	345-353	9
α-helix	378-399	22
α-helix	415-417	3
α-helix	418-423	6
β-strand	428-429	2	4
α-helix	430	1
α-helix	433-434	2
β-strand	440-441	2	4
β-strand	453-460	8	3
β-strand	466-474	9	3
β-strand	478-480	3	3
α-helix	494-496	3
α-helix	502-504	3
β-strand	508-512	5	5
α-helix	513	1
β-strand	520-524	5	5
α-helix	525-526	2
β-strand	529-533	5	6
β-strand	536-539	4	6
α-helix	540-541	2
β-strand	544-546	3	7
β-strand	553-555	3	7
α-helix	556-558	3
β-strand	559	1	8
α-helix	566-591	26
α-helix	596-600	5
α-helix	604-624	21
α-helix	626-627	2
α-helix	631-662	32
α-helix	663-665	3
α-helix	670-671	2
α-helix	678-700	23
β-strand	705-708	4	8
β-strand	718-721	4	8
α-helix	728-748	21
α-helix	754-756	3
α-helix	759-782	24
α-helix	787-818	32
α-helix	821-823	3

Chain B: 35 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	26-28	3	9
β-strand	32-38	7	9
β-strand	41-43	3	10
α-helix	44	1
β-strand	50-53	4	10
α-helix	54	1
α-helix	55-60	6
α-helix	61-72	12
β-strand	84-90	7	9
α-helix	95-106	12
β-strand	138-141	4	9
α-helix	145-158	14
β-strand	162-164	3	9
α-helix	170-173	4
β-strand	181-183	3	9
α-helix	191-201	11
β-strand	206-212	7	11
α-helix	215-229	15
β-strand	234-241	8	11
α-helix	247-257	11
β-strand	265-269	5	11
α-helix	272-284	13
β-strand	290-293	4	11
α-helix	308-311	4
β-strand	315-319	5	11
α-helix	325-331	7
α-helix	345-353	9
α-helix	378-399	22
α-helix	416-417	2
α-helix	418-422	5
α-helix	423-425	3
β-strand	428-429	2	12
α-helix	430	1
β-strand	440-441	2	12
β-strand	453-460	8	11
β-strand	466-474	9	11
β-strand	478-480	3	11
α-helix	495-496	2
α-helix	502-504	3
β-strand	508-511	4	13
β-strand	521-524	4	13
α-helix	525-526	2
β-strand	529-533	5	14
β-strand	536-539	4	14
β-strand	544-546	3	15
β-strand	553-555	3	15
α-helix	556-558	3
α-helix	566-591	26
α-helix	596-600	5
α-helix	605-623	19
α-helix	629-659	31
α-helix	678-700	23
β-strand	705-708	4	16
β-strand	718-721	4	16
α-helix	726-728	3
α-helix	729-748	20
α-helix	758-782	25
α-helix	783-785	3
α-helix	787-805	19
α-helix	806-810	5
α-helix	811-818	8

Chain C: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-30	24
β-strand	34-40	7	17
α-helix	46-52	7
β-strand	185-190	6	17
β-strand	195-200	6	17
α-helix	208-211	4
α-helix	212-215	4
β-strand	220-226	7	17
α-helix	242-255	14
β-strand	263-269	7	17
α-helix	271-280	10
α-helix	296-308	13
β-strand	319-323	5	17
α-helix	331-350	20

Chain D: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
β-strand	47-51	5	18
β-strand	58-63	6	19
β-strand	69-74	6	19
β-strand	78-83	6	19
β-strand	88-94	7	19
α-helix	95	1
β-strand	102-105	4	20
β-strand	111-115	5	20
β-strand	121-125	5	20
β-strand	135-139	5	20
β-strand	146-151	6	21
β-strand	156-161	6	21
β-strand	166-170	5	21
β-strand	176-180	5	21
β-strand	187-192	6	22
β-strand	198-203	6	22
β-strand	208-212	5	22
β-strand	218-222	5	22
β-strand	229-234	6	23
β-strand	240-245	6	23
β-strand	250-254	5	23
β-strand	260-264	5	23
β-strand	273-278	6	24
α-helix	280-282	3
β-strand	284-289	6	24
β-strand	293-298	6	24
β-strand	304-308	5	24
β-strand	315-320	6	18
β-strand	327-331	5	18
β-strand	336-339	4	18

Chain E: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	8-22	15
α-helix	30-43	14
α-helix	53-55	3
α-helix	56-58	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Metabotropic glutamate receptor 2	A, B	protein	855	Homo sapiens	Q14416 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	C	protein	354	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	D	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	E	protein	71	Homo sapiens	P59768 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>7MTS_1 Metabotropic glutamate receptor 2 (chains A, B)
AEGPAKKVLTLEGDLVLGGLFPVHQKGGPAEDCGPVNEHRGIQRLEAMLFALDRINRDPH
LLPGVRLGAHILDSCSKDTHALEQALDFVRASLSRGADGSRHICPDGSYATHGDAPTAIT
GVIGGSYSDVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFFQAKAMAE
ILRFFNWTYVSTVASEGDYGETGIEAFELEARARNICVATSEKVGRAMSRAAFEGVVRAL
LQKPSARVAVLFTRSEDARELLAASQRLNASFTWVASDGWGALESVVAGSEGAAEGAITI
ELASYPISDFASYFQSLDPWNNSRNPWFREFWEQRFRCSFRQRDCAAHSLRAVPFEQESK
IMFVVNAVYAMAHALHNMHRALCPNTTRLCDAMRPVNGRRLYKDFVLNVKFDAPFRPADT
HNEVRFDRFGDGIGRYNIFTYLRAGSGRYRYQKVGYWAEGLTLDTSLIPWASPSAGPLPA
SRCSEPCLQNEVKSVQPGEVCCWLCIPCQPYEYRLDEFTCADCGLGYWPNASLTGCFELP
QEYIRWGDAWAVGPVTIACLGALATLFVLGVFVRHNATPVVKASGRELCYILLGGVFLCY
CMTFIFIAKPSTAVCTLRRLGLGTAFSVCYSALLTKTNRIARIFGGAREGAQRPRFISPA
SQVAICLALISGQLLIVVAWLVVEAPGTGKETAPERREVVTLRCNHRDASMLGSLAYNVL
LIALCTLYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFLPIFYVTSSDYRVQTTTMCVS
VSLSGSVVLGCLFAPKLHIILFQPQKNVVSHRAPTSRFGSAAARASSSLGQGSGSQFVPT
VCNGREVVDSTTSSL

Sequence of entity 2 (C), FASTA

>7MTS_2 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains C)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 3 (D), FASTA

>7MTS_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains D)
GSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (E), FASTA

>7MTS_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains E)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Ligands and cofactors

ID	Name	Formula	Copies
ZQY	2-methoxy-6-propyl-N-(2-{4-[(1H-tetrazol-5-yl)methoxy]phenyl}ethyl)thieno[2,3-d…	C20 H23 N7 O2 S	1
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	2
GLU	Glutamic acid	C5 H9 N O4	2

Primary citation

G-protein activation by a metabotropic glutamate receptor. Seven, A.B., Barros-Alvarez, X., de Lapeyriere, M. et al. Nature (2021) 595:450-454. DOI 10.1038/s41586-021-03680-3 · PubMed

Other PDB entries of the same protein (UniProt Q14416 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4XAQ 2.21 Å, mGluR2 ECD and mGluR3 ECD with ligands
4XAS 2.35 Å, mGluR2 ECD ligand complex
7EPE 2.5 Å, Crystal structure of mGlu2 bound to NAM563
5CNJ 2.65 Å, mGlur2 with glutamate analog
5CNI 2.69 Å, mGlu2 with Glutamate
7EPF 2.7 Å, Crystal structure of mGlu2 bound to NAM597
5KZN 2.8 Å, Metabotropic Glutamate Receptor
5KZQ 2.8 Å, Metabotropic Glutamate Receptor in complex with antagonist…
8JCU 2.8 Å, Cryo-EM structure of mGlu2-mGlu3 heterodimer in presence of LY341495 (dimerization mode I)
8JD2 2.8 Å, Cryo-EM structure of G protein-free mGlu2-mGlu3 heterodimer in Acc state
8JCY 2.9 Å, Cryo-EM structure of mGlu2-mGlu3 heterodimer in presence of LY341495, NAM563, and…
8JD4 2.9 Å, Cryo-EM structure of G protein-free mGlu2-mGlu4 heterodimer in Acc state

Browse structure collections

About this viewer

MolViewer shows 7MTS directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.