7NTJ: PALS1 PDZ1 domain with SARS-CoV-1_E PBM complex

PALS1 PDZ1 domain with SARS-CoV-1_E PBM complex. Determined by X-ray diffraction at 1.74 Å resolution. Released 16 Jun 2021.

Method: X-ray diffraction
Resolution: 1.74 Å
Organisms: Homo sapiens, Severe acute respiratory syndrome coronavirus
Chains: 4
Atoms: 1,686
Mol. weight: 20.47 kDa
Released: 16 Jun 2021

Explore 7NTJ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7NTJ contains 6 α-helices and 15 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 2 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	255-261	7	2
β-strand	269-274	6	2
β-strand	277-283	7	2
α-helix	288-292	5
β-strand	300-304	5	2
β-strand	307-308	2	2
α-helix	314-322	9
β-strand	324	1	1
β-strand	326-333	8	2

Chain B: 3 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	251-253	3
β-strand	255-261	7	1
β-strand	269-274	6	1
β-strand	277-283	7	1
α-helix	288-292	5
β-strand	300-304	5	1
β-strand	307-308	2	1
α-helix	314-322	9
β-strand	326-333	8	1

Chain C: 1 helix, 1 β-strand

Element	Residues	Length	Sheet
α-helix	206-208	3
β-strand	209-210	2	2

Chain G: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	209-210	2	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
MAGUK p55 subfamily member 5	A, B	protein	87	Homo sapiens	Q8N3R9 (AlphaFold model)
Envelope small membrane protein	C, G	protein	8	Severe acute respiratory syndrome coronavirus	P59637 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>7NTJ_1 MAGUK p55 subfamily member 5 (chains A, B)
GPLGSKIVRIEKARDIPLGATVRNEMDSVIISRIVKGGAAEKSGLLHEGDEVLEINGIEI
RGKDVNEVFDLLSDMHGTLTFVLIPSQ

Sequence of entity 2 (C, G), FASTA

>7NTJ_2 Envelope small membrane protein (chains C, G)
EGVPDLLV

Primary citation

Structural basis of coronavirus E protein interactions with human PALS1 PDZ domain. Javorsky, A., Humbert, P.O., Kvansakul, M. Commun Biol (2021) 4:724-724. DOI 10.1038/s42003-021-02250-7 · PubMed

Other PDB entries of the same protein (UniProt Q8N3R9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4UU5 1.23 Å, Crystal structure of the PDZ domain of PALS1 in complex with the crb peptide
4UU6 1.8 Å, Crystal structure of the PDZ domain of PALS1
7NTK 1.9 Å, PALS1 PDZ1 domain with SARS-CoV-2_E PBM complex
3UIT 2.05 Å, Overall structure of Patj/Pals1/Mals complex
7QCS 2.8 Å, PALS1/MPP5 PDZ domain in complex with SARS-CoV-2_E PBM peptide
4WSI 2.95 Å, Crystal Structure of PALS1/Crb complex
7M4R 3.65 Å, Structural basis for SARS-CoV-2 envelope protein in recognition of human cell junction…
1Y76 Solution Structure of Patj/Pals1 L27 Domain Complex

Browse structure collections

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