7P30: DNA replication licensing factor MCM2

Name: PDB 7P30: 3.0 A resolution structure of a DNA-loaded MCM double hexamer
Published: 2022-02-02
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: replication

3.0 A resolution structure of a DNA-loaded MCM double hexamer. Determined by electron microscopy at 3.0 Å resolution. Released 2 Feb 2022.

Method: Electron microscopy
Resolution: 3.0 Å
Organisms: Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae S288C
Chains: 14
Atoms: 62,747
Mol. weight: 1258.86 kDa
Ligands: ATP, ADP, ZN, MG
Released: 2 Feb 2022

Explore 7P30 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7P30 contains 377 α-helices and 338 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 2: 29 helices, 24 β-strands

Element	Residues	Length	Sheet
α-helix	194-197	4
α-helix	201-217	17
α-helix	226-237	12
β-strand	241-245	5	1
α-helix	246-262	17
α-helix	264-282	19
α-helix	286-288	3
β-strand	294-298	5	1
β-strand	316-327	12	2
β-strand	331-341	11	2
β-strand	347-352	6	2
β-strand	373-375	3	2
β-strand	381-391	11	2
α-helix	394-396	3
α-helix	398	1
β-strand	405-411	7	2
α-helix	412-414	3
β-strand	423-432	10	2
β-strand	446	1	3
β-strand	449-458	10	2
α-helix	478-489	12
α-helix	493-498	6
α-helix	509-520	12
β-strand	525-526	2	4
β-strand	532-533	2	4
α-helix	536-538	3
β-strand	539-543	5	5
α-helix	549-559	11
β-strand	563-567	5	5
β-strand	579-582	4	6
β-strand	589-592	4	6
α-helix	595-598	4
β-strand	603-607	5	5
α-helix	609-611	3
α-helix	614-626	13
β-strand	628-633	6	7
β-strand	636-641	6	7
β-strand	645-650	6	5
α-helix	653-655	3
α-helix	663-665	3
α-helix	671-674	4
β-strand	679-683	5	5
α-helix	689-706	18
α-helix	757-759	3
α-helix	760-773	14
β-strand	777	1	8
α-helix	780-782	3
α-helix	783-800	18
α-helix	804-805	2
α-helix	807-823	17
β-strand	828	1	8
α-helix	831-847	17
α-helix	851-860	10

Chain 3: 28 helices, 26 β-strands

Element	Residues	Length	Sheet
α-helix	19-34	16
α-helix	37-51	15
β-strand	95-99	5	9
α-helix	100-106	7
α-helix	108-116	9
α-helix	118-136	19
β-strand	154-158	5	9
α-helix	162-164	3
β-strand	165-166	2	10
α-helix	168-170	3
α-helix	173-175	3
β-strand	179-188	10	10
β-strand	193-202	10	10
β-strand	209-213	5	10
α-helix	229-232	4
β-strand	234	1	11
β-strand	240	1	11
β-strand	242-243	2	10
β-strand	249-259	11	10
α-helix	266	1
β-strand	273-279	7	10
α-helix	280-282	3
β-strand	291-301	11	10
β-strand	318-329	12	10
α-helix	336-338	3
α-helix	341-343	3
α-helix	344-353	10
α-helix	359-366	8
α-helix	375-386	12
β-strand	391-392	2	12
β-strand	398-399	2	12
β-strand	405-409	5	13
α-helix	415-425	11
β-strand	429-433	5	13
α-helix	440-443	4
β-strand	447-449	3	14
β-strand	455-457	3	14
α-helix	461-464	4
β-strand	469-473	5	13
α-helix	475-477	3
α-helix	480-491	12
β-strand	494-499	6	15
β-strand	502-507	6	15
β-strand	511-516	6	13
α-helix	529-533	5
α-helix	537-542	6
β-strand	545-549	5	13
α-helix	555-569	15
α-helix	653-666	14
β-strand	670	1	16
α-helix	673-687	15
α-helix	699-715	17
β-strand	720	1	16
α-helix	723-737	15

Chain 4: 37 helices, 35 β-strands

Element	Residues	Length	Sheet
β-strand	179	1	17
α-helix	180	1
β-strand	186	1	17
α-helix	187-199	13
α-helix	225-236	12
β-strand	240-243	4	18
α-helix	245-249	5
α-helix	253-255	3
α-helix	257-263	7
α-helix	265-287	23
α-helix	294-298	5
β-strand	302-305	4	18
α-helix	314-316	3
α-helix	319-321	3
β-strand	325-335	11	19
α-helix	336-338	3
β-strand	339-349	11	19
β-strand	355-359	5	19
β-strand	361	1	20
β-strand	364-367	4	20
β-strand	370	1	21
β-strand	379	1	21
β-strand	382-384	3	19
α-helix	386-388	3
β-strand	390-401	12	19
α-helix	410-412	3
β-strand	414-420	7	19
α-helix	421-423	3
β-strand	432-442	11	19
β-strand	445	1	22
β-strand	453-454	2	22
β-strand	456	1	23
β-strand	458-467	10	19
α-helix	474-477	4
α-helix	478-480	3
α-helix	482-491	10
α-helix	503-513	11
α-helix	518-525	8
α-helix	534-545	12
α-helix	548-549	2
β-strand	550-551	2	24
β-strand	557-558	2	24
β-strand	564-568	5	25
α-helix	576-584	9
β-strand	590-592	3	25
β-strand	596	1	26
α-helix	598-602	5
β-strand	603-607	5	27
β-strand	614-618	5	27
α-helix	620-623	4
β-strand	628-632	5	25
α-helix	634-636	3
α-helix	639-643	5
α-helix	645-651	7
β-strand	653-658	6	28
β-strand	661-666	6	28
β-strand	670-675	6	25
α-helix	688-690	3
α-helix	698-700	3
β-strand	704-708	5	25
α-helix	714-725	12
α-helix	726-728	3
α-helix	744-757	14
β-strand	761-762	2	29
α-helix	764-779	16
α-helix	795-810	16
α-helix	811-813	3
β-strand	816-817	2	29
α-helix	819-833	15
α-helix	835-837	3
β-strand	838	1	30
β-strand	845	1	30

Chain 5: 32 helices, 28 β-strands

Element	Residues	Length	Sheet
β-strand	8-12	5	31
α-helix	18-20	3
α-helix	24-37	14
α-helix	45-56	12
β-strand	60-64	5	32
α-helix	65-71	7
α-helix	73-81	9
α-helix	83-107	25
α-helix	134-135	2
β-strand	136-140	5	32
β-strand	146	1	33
α-helix	148-150	3
α-helix	153-155	3
β-strand	159-169	11	33
β-strand	173-183	11	33
β-strand	189-193	5	33
α-helix	220-225	6
α-helix	232-234	3
β-strand	241-243	3	33
α-helix	245-247	3
β-strand	249-259	11	33
α-helix	265-266	2
α-helix	270-272	3
β-strand	273-279	7	33
α-helix	280-282	3
β-strand	291-301	11	33
β-strand	323	1	2
β-strand	325-335	11	33
α-helix	351-361	11
α-helix	366-373	8
α-helix	382-393	12
β-strand	398-399	2	34
β-strand	405-406	2	34
β-strand	412-416	5	35
α-helix	422-432	11
β-strand	436-440	5	35
β-strand	444	1	36
β-strand	447	1	36
β-strand	452-455	4	37
β-strand	462-466	5	37
α-helix	468-471	4
β-strand	476-480	5	35
α-helix	487-499	13
β-strand	501-505	5	38
β-strand	510-514	5	38
β-strand	518-523	6	35
α-helix	526-528	3
α-helix	536-539	4
α-helix	544-548	5
β-strand	552-556	5	35
α-helix	562-577	16
α-helix	579-582	4
α-helix	585-588	4
α-helix	596-609	14
β-strand	613	1	39
α-helix	616-638	23
α-helix	650-665	16
β-strand	671	1	39
α-helix	674-687	14
α-helix	688-693	6

Chain 6: 27 helices, 27 β-strands

Element	Residues	Length	Sheet
α-helix	101-103	3
α-helix	106-120	15
α-helix	135-146	12
β-strand	150-154	5	40
α-helix	155-159	5
α-helix	162-164	3
α-helix	165-172	8
α-helix	174-192	19
β-strand	264-268	5	40
β-strand	274	1	3
α-helix	276-278	3
α-helix	281-283	3
β-strand	287-297	11	3
β-strand	301-311	11	3
β-strand	317-322	6	3
β-strand	344-346	3	3
β-strand	352-362	11	3
α-helix	365-367	3
α-helix	373-375	3
β-strand	376-382	7	3
α-helix	383-385	3
β-strand	394-405	12	3
α-helix	407-409	3
β-strand	418-421	4	20
β-strand	446-447	2	20
β-strand	450-461	12	3
α-helix	513-520	8
α-helix	525-531	7
α-helix	541-547	7
β-strand	557-558	2	41
β-strand	564-565	2	41
β-strand	571-574	4	42
α-helix	581-591	11
β-strand	595-599	5	42
β-strand	603	1	28
β-strand	610-613	4	43
β-strand	622-625	4	43
β-strand	635-639	5	42
α-helix	641-643	3
α-helix	646-657	12
β-strand	660-665	6	44
β-strand	668-673	6	44
β-strand	677-682	6	42
α-helix	695-698	4
α-helix	703-706	4
β-strand	711-714	4	42
α-helix	721-737	17
α-helix	738-740	3
α-helix	748-758	11
β-strand	764	1	45
α-helix	767-784	18
α-helix	797-813	17
β-strand	818	1	45
α-helix	821-833	13

Chain 7: 36 helices, 30 β-strands

Element	Residues	Length	Sheet
β-strand	7-8	2	10
α-helix	14-27	14
α-helix	62-71	10
β-strand	77-81	5	46
α-helix	82-94	13
α-helix	102-108	7
α-helix	110-122	13
α-helix	125-128	4
α-helix	138-164	27
α-helix	179-188	10
α-helix	194-196	3
β-strand	201-205	5	46
α-helix	209-215	7
α-helix	220-222	3
β-strand	225	1	23
α-helix	232-234	3
β-strand	238-248	11	23
β-strand	252-261	10	23
β-strand	267-272	6	23
β-strand	277-278	2	22
β-strand	283	1	47
α-helix	287-291	5
α-helix	295-296	2
β-strand	297	1	47
α-helix	298	1
β-strand	299-300	2	23
β-strand	306-316	11	23
α-helix	319-321	3
β-strand	330-336	7	23
α-helix	337-339	3
β-strand	348-359	12	23
α-helix	364-366	3
β-strand	371	1	10
β-strand	372-383	12	23
α-helix	395-406	12
α-helix	410-416	7
α-helix	426-437	12
β-strand	442-445	4	48
β-strand	448-450	3	48
β-strand	456-460	5	49
α-helix	466-476	11
β-strand	481-484	4	49
α-helix	485-487	3
β-strand	488	1	15
α-helix	490-492	3
β-strand	495-499	5	50
β-strand	506-510	5	50
α-helix	512-515	4
β-strand	520-524	5	49
α-helix	526-528	3
α-helix	531-543	13
β-strand	545-550	6	26
β-strand	553-558	6	26
β-strand	562-567	6	49
α-helix	580-582	3
α-helix	588-592	5
β-strand	596-600	5	49
α-helix	606-621	16
α-helix	632-634	3
α-helix	635-645	11
β-strand	651	1	51
α-helix	654-676	23
α-helix	686-702	17
β-strand	707	1	51
α-helix	710-722	13
α-helix	725-728	4

Chain A: 29 helices, 24 β-strands

Element	Residues	Length	Sheet
α-helix	194-198	5
α-helix	201-217	17
α-helix	226-237	12
β-strand	241-245	5	52
α-helix	246-262	17
α-helix	264-282	19
α-helix	286-288	3
β-strand	294-298	5	52
β-strand	316-327	12	53
β-strand	331-341	11	53
β-strand	347-352	6	53
β-strand	373-375	3	53
β-strand	381-391	11	53
α-helix	394-396	3
α-helix	398	1
β-strand	405-411	7	53
α-helix	412-414	3
β-strand	423-432	10	53
β-strand	446	1	54
β-strand	449-458	10	53
α-helix	478-489	12
α-helix	493-498	6
α-helix	509-520	12
β-strand	525-526	2	55
β-strand	532-533	2	55
α-helix	536-538	3
β-strand	539-543	5	56
α-helix	550-559	10
β-strand	563-567	5	56
β-strand	579-582	4	57
β-strand	589-592	4	57
α-helix	595-598	4
β-strand	603-607	5	56
α-helix	609-611	3
α-helix	614-626	13
β-strand	628-633	6	58
β-strand	636-641	6	58
β-strand	645-650	6	56
α-helix	653-655	3
α-helix	663-665	3
α-helix	671-674	4
β-strand	679-683	5	56
α-helix	689-706	18
α-helix	757-759	3
α-helix	760-773	14
β-strand	777	1	59
α-helix	780-782	3
α-helix	783-800	18
α-helix	804-805	2
α-helix	807-823	17
β-strand	828	1	59
α-helix	831-847	17
α-helix	851-860	10

Chain B: 29 helices, 26 β-strands

Element	Residues	Length	Sheet
α-helix	19-34	16
α-helix	37-51	15
β-strand	95-99	5	60
α-helix	100-106	7
α-helix	108-116	9
α-helix	118-136	19
β-strand	154-158	5	60
α-helix	162-164	3
β-strand	165-166	2	61
α-helix	168-170	3
α-helix	173-175	3
β-strand	179-188	10	61
β-strand	193-202	10	61
β-strand	209-213	5	61
α-helix	229-232	4
β-strand	234	1	62
β-strand	240	1	62
β-strand	242-243	2	61
β-strand	249-259	11	61
α-helix	266	1
β-strand	273-279	7	61
α-helix	280-282	3
β-strand	291-301	11	61
β-strand	318-329	12	61
α-helix	336-338	3
α-helix	341-343	3
α-helix	344-353	10
α-helix	359-366	8
α-helix	375-386	12
β-strand	391-392	2	63
β-strand	398-399	2	63
β-strand	405-409	5	64
α-helix	415-425	11
β-strand	429-433	5	64
α-helix	440-443	4
β-strand	447-449	3	65
β-strand	455-457	3	65
α-helix	461-464	4
β-strand	469-473	5	64
α-helix	475-477	3
α-helix	480-491	12
β-strand	494-499	6	66
β-strand	502-507	6	66
β-strand	511-516	6	64
α-helix	529-533	5
α-helix	537-542	6
β-strand	545-549	5	64
α-helix	555-568	14
α-helix	653-666	14
β-strand	670	1	67
α-helix	673-687	15
α-helix	691-693	3
α-helix	699-716	18
β-strand	720	1	67
α-helix	723-737	15

4 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA replication licensing factor MCM2	2, A	protein	868	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P29469 (AlphaFold model)
DNA replication licensing factor MCM3	3, B	protein	1006	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P24279 (AlphaFold model)
DNA replication licensing factor MCM4	4, C	protein	933	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P30665 (AlphaFold model)
Minichromosome maintenance protein 5	5, D	protein	775	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P29496 (AlphaFold model)
DNA replication licensing factor MCM6	6, E	protein	1017	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P53091
DNA replication licensing factor MCM7	7, F	protein	845	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	P38132
DNA (53-mer)	X	DNA	53	Saccharomyces cerevisiae S288C
DNA (53-mer)	Y	DNA	53	Saccharomyces cerevisiae S288C

Sequence of entity 1 (2, A), FASTA

>7P30_1 DNA replication licensing factor MCM2 (chains 2, A)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH

Sequence of entity 2 (3, B), FASTA

>7P30_2 DNA replication licensing factor MCM3 (chains 3, B)
MKRRWKKNFIAVSAANRFKKISSSGALENLYFQGEMEGSTGFDGDATTFFAPDAVFGDRV
RRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQDDADERDLLGDDDGDDLEKEKKAAS
STSLNILPHRIIISLDDLREFDRSFWSGILVEPAYFIPPAEKALTDLADSMDDVPHPNAS
AVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLVSVEGIVTKTSLVRPKLIRSVHYAAK
TGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNKLTTEYGYSTFIDHQRITVQEMPEMA
PAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKSLGAGGMNQSNSNTLIGFKTLILGNT
VYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIFDILSQSLAPSIYGHDHIKKAILLML
MGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLRFVLNTASLAIATTGRGSSGVGLTAA
VTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTDVDRVAIHEVMEQQTVTIAKAGIHTT
LNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLLSRFDLLFVVTDDINEIRDRSISEHV
LRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINPEEHSNSGAGVENEGEDDEDHVFEKF
NPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYVQYAKERVIPQLTQEAINVIVKNYTD
LRNDDNTKKSPITARTLETLIRLATAHAKVRLSKTVNKVDAKVAANLLRFALLGEDIGND
IDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSGSPIKSTPRRSTASSVNATPSSARRI
LRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQLGLRVSPRRREHLHAPEEGSSGPLTE
VGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTISTGRLSLISGIIARLMQTEIFEEESYP
VASLFERINEELPEEEKFSAQEYLAGLKIMSDRNNLMVADDKVWRV

Sequence of entity 3 (4, C), FASTA

>7P30_3 DNA replication licensing factor MCM4 (chains 4, C)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV

Sequence of entity 4 (5, D), FASTA

>7P30_4 Minichromosome maintenance protein 5 (chains 5, D)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV

Sequence of entity 5 (6, E), FASTA

>7P30_5 DNA replication licensing factor MCM6 (chains 6, E)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS

Sequence of entity 6 (7, F), FASTA

>7P30_6 DNA replication licensing factor MCM7 (chains 7, F)
MSAALPSIQLPVDYNNLFNEITDFLVTFKQDTLSSDATRNENEDENLDAENIEQHLLEKG
PKYMAMLQKVANRELNSVIIDLDDILQYQNEKFLQGTQADDLVSAIQQNANHFTELFCRA
IDNNMPLPTKEIDYKDDVLDVILNQRRLRNERMLSDRTNEIRSENLMDTTMDPPSSMNDA
LREVVEDETELFPPNLTRRYFLYFKPLSQNCARRYRKKAISSKPLSVRQIKGDFLGQLIT
VRGIITRVSDVKPAVEVIAYTCDQCGYEVFQEVNSRTFTPLSECTSEECSQNQTKGQLFM
STRASKFSAFQECKIQELSQQVPVGHIPRSLNIHVNGTLVRSLSPGDIVDVTGIFLPAPY
TGFKALKAGLLTETYLEAQFVRQHKKKFASFSLTSDVEERVMELITSGDVYNRLAKSIAP
EIYGNLDVKKALLLLLVGGVDKRVGDGMKIRGDINVCLMGDPGVAKSQLLKAICKISPRG
VYTTGKGSSGVGLTAAVMKDPVTDEMILEGGALVLADNGICCIDEFDKMDESDRTAIHEV
MEQQTISISKAGINTTLNARTSILAAANPLYGRYNPRLSPLDNINLPAALLSRFDILFLM
LDIPSRDDDEKLAEHVTYVHMHNKQPDLDFTPVEPSKMREYIAYAKTKRPVMSEAVNDYV
VQAYIRLRQDSKREMDSKFSFGQATPRTLLGIIRLSQALAKLRLADMVDIDDVEEALRLV
RVSKESLYQETNKSKEDESPTTKIFTIIKKMLQETGKNTLSYENIVKTVRLRGFTMLQLS
NCIQEYSYLNVWHLINEGNTLKFVDDGTMDTDQEDSLVSTPKLAPQTTASANVSAQDSDI
DLQDA

Sequence of entity 7 (X), FASTA

>7P30_7 DNA (53-MER) (chains X)
GCATGCATGCGCATGCATGCATGCATGCTGCATGCATGCATGCGCATGCATGC

Sequence of entity 8 (Y), FASTA

>7P30_8 DNA (53-MER) (chains Y)
GCATGCATGCGCATGCATGCATGCAGCATGCATGCATGCATGCGCATGCATGC

Ligands and cofactors

ID	Name	Formula	Copies
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	2
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	8
ZN	Zinc ion	Zn	10
MG	Magnesium ion	Mg	10

Primary citation

Structural mechanism for the selective phosphorylation of DNA-loaded MCM double hexamers by the Dbf4-dependent kinase. Greiwe, J.F., Miller, T.C.R., Locke, J. et al. Nat Struct Mol Biol (2022) 29:10-20. DOI 10.1038/s41594-021-00698-z · PubMed

Other PDB entries of the same protein (UniProt P29469 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7W8G 2.52 Å, Cryo-EM structure of MCM double hexamer
28VY 2.7 Å, sCMGE assembled on ARS1 DNA with Sld2 and RPA
8RIF 2.79 Å, Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
7V3V 2.9 Å, Cryo-EM structure of MCM double hexamer bound with DDK in State I
9TH8 2.9 Å, Cryo-EM structure of MCM2-7 DH bound to Sld3-Sld7, Cdc45 and DNA
8KG6 3.07 Å, Yeast replisome in state I
9RHL 3.1 Å, Phospho-DH bound by Sld3-MBD on ARS1 DNA
7PMK 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
7PMN 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
7PT6 3.2 Å, Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III
7V3U 3.2 Å, Cryo-EM structure of MCM double hexamer with structured Mcm4-NSD
9E2Y 3.2 Å, Cryo-EM structure of yeast CMG helicase stalled at G4-containing DNA template, state 3

Browse structure collections

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