9E2Y: DNA replication complex GINS protein PSF1

Cryo-EM structure of yeast CMG helicase stalled at G4-containing DNA template, state 3. Determined by electron microscopy at 3.2 Å resolution. Released 26 Mar 2025.

Method: Electron microscopy
Resolution: 3.2 Å
Organisms: Saccharomyces cerevisiae W303, synthetic construct
Chains: 14
Atoms: 41,877
Mol. weight: 809.69 kDa
Ligands: ATP, ZN, MG, ADP
Released: 26 Mar 2025

Explore 9E2Y in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9E2Y contains 252 α-helices and 225 β-strands across 12 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 2: 35 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	176-178	3
α-helix	183-187	5
α-helix	194-199	6
α-helix	201-217	17
β-strand	219	1	16
β-strand	225	1	16
α-helix	226-236	11
β-strand	241-244	4	17
α-helix	246-252	7
α-helix	254-262	9
α-helix	264-282	19
α-helix	286-288	3
β-strand	294-297	4	17
β-strand	304	1	18
α-helix	311-313	3
β-strand	317-327	11	18
β-strand	331-334	4	18
β-strand	336-337	2	19
β-strand	339-341	3	20
β-strand	347	1	20
α-helix	348-350	3
β-strand	351-352	2	19
α-helix	353-354	2
β-strand	373-375	3	20
β-strand	382-391	10	18
α-helix	398	1
α-helix	402-404	3
β-strand	405-411	7	18
β-strand	423-433	11	18
α-helix	436-442	7
β-strand	446	1	21
α-helix	447	1
β-strand	448-458	11	18
α-helix	493-499	7
α-helix	509-520	12
β-strand	525-527	3	22
β-strand	531-533	3	22
β-strand	539-542	4	23
α-helix	550-559	10
β-strand	563-567	5	23
β-strand	571	1	24
β-strand	578-582	5	25
β-strand	589-593	5	25
α-helix	595-598	4
β-strand	602-607	6	23
α-helix	609-611	3
α-helix	614-617	4
α-helix	618-620	3
α-helix	621-626	6
β-strand	630-633	4	26
β-strand	636-639	4	26
β-strand	644-650	7	23
α-helix	653-655	3
α-helix	663-666	4
α-helix	671-674	4
β-strand	679-682	4	23
α-helix	689-705	17
α-helix	740-753	14
α-helix	760-773	14
β-strand	777	1	27
α-helix	783-797	15
α-helix	803-804	2
α-helix	807-823	17
β-strand	828	1	27
α-helix	831-847	17
α-helix	851-860	10

Chain 3: 27 helices, 27 β-strands

Element	Residues	Length	Sheet
α-helix	20-34	15
α-helix	36-51	16
β-strand	95-99	5	28
α-helix	100-106	7
α-helix	108-116	9
α-helix	118-136	19
α-helix	144-148	5
β-strand	154-158	5	28
β-strand	165-166	2	29
α-helix	173-175	3
β-strand	179-189	11	29
β-strand	195-203	9	30
β-strand	208-213	6	30
α-helix	230-232	3
β-strand	234	1	31
β-strand	240	1	31
β-strand	241-251	11	30
β-strand	253-259	7	29
α-helix	262-264	3
β-strand	273-279	7	29
α-helix	280-282	3
β-strand	291-301	11	29
β-strand	318-328	11	29
α-helix	340-343	4
α-helix	344-354	11
α-helix	359-366	8
α-helix	375-386	12
β-strand	391-392	2	32
β-strand	398-399	2	32
β-strand	405-409	5	33
α-helix	415-425	11
β-strand	429-433	5	33
β-strand	437	1	34
α-helix	439-443	5
β-strand	444-448	5	35
β-strand	455-459	5	35
α-helix	461-464	4
β-strand	469-473	5	33
α-helix	475-477	3
α-helix	480-492	13
β-strand	494-499	6	36
β-strand	502-507	6	36
β-strand	511-516	6	33
α-helix	529-532	4
α-helix	537-540	4
β-strand	545-549	5	33
α-helix	555-569	15
α-helix	649-652	4
α-helix	653-665	13
β-strand	670	1	37
α-helix	673-687	15
α-helix	699-715	17
β-strand	720	1	37
α-helix	723-738	16

Chain 4: 31 helices, 34 β-strands

Element	Residues	Length	Sheet
β-strand	178-180	3	38
β-strand	186	1	38
α-helix	187-199	13
β-strand	202	1	39
α-helix	203-209	7
α-helix	213-215	3
β-strand	224	1	39
α-helix	225-236	12
β-strand	240-244	5	40
α-helix	246-250	5
α-helix	252-254	3
α-helix	255-263	9
α-helix	265-287	23
α-helix	293-297	5
β-strand	302-306	5	40
α-helix	314-316	3
α-helix	319-321	3
β-strand	325-335	11	41
α-helix	336-338	3
β-strand	339-349	11	41
β-strand	355-359	5	41
β-strand	360-361	2	42
β-strand	364-365	2	42
β-strand	370	1	43
β-strand	379	1	43
β-strand	382-384	3	41
α-helix	386-388	3
β-strand	390-401	12	41
α-helix	403-405	3
α-helix	411-413	3
β-strand	414-420	7	41
α-helix	421-423	3
β-strand	432-442	11	41
β-strand	445	1	44
β-strand	453-454	2	44
β-strand	456	1	45
α-helix	457	1
β-strand	458-468	11	41
α-helix	503-513	11
α-helix	518-526	9
α-helix	534-545	12
α-helix	547-549	3
β-strand	550	1	46
β-strand	558	1	46
β-strand	563-568	6	47
α-helix	575-584	10
β-strand	588-591	4	47
β-strand	604-608	5	48
β-strand	613-617	5	48
α-helix	620-623	4
β-strand	628-631	4	47
α-helix	639-651	13
β-strand	653-656	4	49
β-strand	663-666	4	49
β-strand	670-675	6	47
α-helix	688-692	5
α-helix	696-699	4
β-strand	704-708	5	47
α-helix	714-726	13
α-helix	744-757	14
β-strand	761	1	50
α-helix	764-779	16
α-helix	795-811	17
β-strand	816	1	50
α-helix	819-835	17

Chain 5: 28 helices, 26 β-strands

Element	Residues	Length	Sheet
α-helix	23-37	15
β-strand	39	1	51
β-strand	44	1	51
α-helix	45-55	11
β-strand	60-64	5	52
α-helix	65-71	7
α-helix	73-81	9
α-helix	83-102	20
α-helix	133-135	3
β-strand	136-140	5	52
α-helix	143-147	5
α-helix	148-150	3
α-helix	153-155	3
β-strand	158-169	12	53
α-helix	170-172	3
β-strand	173-183	11	53
β-strand	189-193	5	53
β-strand	241-259	19	53
β-strand	273-279	7	53
α-helix	280-282	3
β-strand	291-301	11	53
β-strand	323	1	18
β-strand	325-335	11	53
α-helix	339-343	5
α-helix	351-361	11
α-helix	366-373	8
α-helix	382-393	12
β-strand	398-399	2	54
β-strand	405-406	2	54
β-strand	412-416	5	55
α-helix	422-432	11
β-strand	436-440	5	55
β-strand	444	1	26
β-strand	451-455	5	56
β-strand	462-466	5	56
α-helix	468-471	4
β-strand	476-480	5	55
α-helix	487-491	5
α-helix	494-498	5
β-strand	501-506	6	34
β-strand	509-514	6	34
β-strand	518-523	6	55
α-helix	536-539	4
α-helix	544-548	5
β-strand	552-556	5	55
α-helix	562-577	16
α-helix	596-609	14
β-strand	613-614	2	57
α-helix	616-636	21
α-helix	650-665	16
β-strand	671-672	2	57
α-helix	674-684	11
α-helix	685-689	5
α-helix	690-693	4

Chain 6: 31 helices, 33 β-strands

Element	Residues	Length	Sheet
α-helix	99-101	3
α-helix	103-104	2
α-helix	106-121	16
β-strand	123	1	58
β-strand	134	1	58
α-helix	136-146	11
β-strand	150-154	5	59
α-helix	155-160	6
α-helix	161-164	4
α-helix	165-172	8
α-helix	174-192	19
α-helix	194-196	3
β-strand	198	1	60
β-strand	260	1	60
β-strand	264-268	5	59
β-strand	274	1	21
α-helix	276-278	3
β-strand	287-297	11	21
β-strand	301-311	11	21
β-strand	317-322	6	21
β-strand	344-346	3	21
β-strand	352-362	11	21
α-helix	365-367	3
α-helix	369	1
α-helix	373-375	3
β-strand	376-382	7	21
α-helix	383-385	3
β-strand	394-405	12	21
α-helix	407-410	4
β-strand	412	1	61
β-strand	415	1	61
β-strand	437	1	62
β-strand	438	1	42
β-strand	448	1	62
β-strand	450-461	12	21
α-helix	501-507	7
α-helix	510-521	12
α-helix	525-531	7
α-helix	541-552	12
β-strand	557-558	2	63
β-strand	564-565	2	63
β-strand	571-575	5	64
α-helix	581-591	11
β-strand	595-598	4	64
β-strand	611-614	4	65
β-strand	621-624	4	65
α-helix	627-630	4
β-strand	635-638	4	64
α-helix	641-643	3
α-helix	646-657	12
β-strand	660-665	6	24
β-strand	668-673	6	24
β-strand	677-682	6	64
α-helix	695-698	4
α-helix	703-706	4
β-strand	711-715	5	64
α-helix	721-737	17
α-helix	738-741	4
α-helix	748-758	11
β-strand	764-765	2	66
α-helix	767-784	18
α-helix	797-813	17
β-strand	818-819	2	66
α-helix	821-833	13

Chain 7: 27 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	7-8	2	30
α-helix	14-27	14
α-helix	62-71	10
β-strand	77-81	5	67
α-helix	82-95	14
α-helix	102-108	7
α-helix	111-123	13
α-helix	125-128	4
α-helix	138-155	18
α-helix	194-197	4
β-strand	201-205	5	67
α-helix	209-214	6
β-strand	224-225	2	45
α-helix	232-234	3
β-strand	238-248	11	45
α-helix	249-251	3
β-strand	252-262	11	45
β-strand	270-272	3	45
β-strand	277-278	2	44
β-strand	283	1	68
α-helix	287-292	6
β-strand	297	1	68
β-strand	298-316	19	45
α-helix	319-321	3
β-strand	330-336	7	45
α-helix	337-339	3
β-strand	348-358	11	45
α-helix	362-368	7
β-strand	371	1	30
β-strand	373-383	11	45
α-helix	410-418	9
α-helix	426-437	12
β-strand	456-460	5	69
β-strand	462	1	70
β-strand	464	1	70
α-helix	467-476	10
β-strand	481-484	4	69
β-strand	520-524	5	69
α-helix	531-543	13
β-strand	545-547	3	71
β-strand	556-558	3	71
β-strand	562-567	6	69
α-helix	580-583	4
α-helix	588-593	6
β-strand	596-600	5	69
α-helix	606-622	17
α-helix	631-634	4
α-helix	635-647	13
β-strand	651	1	72
α-helix	654-675	22
α-helix	686-703	18
β-strand	707	1	72
α-helix	710-728	19

Chain A: 8 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	4-22	19
α-helix	29-32	4
α-helix	34-59	26
α-helix	65-103	39
α-helix	120-122	3
α-helix	125-143	19
α-helix	157-158	2
β-strand	162-167	6	1
β-strand	173-175	3	2
β-strand	180-182	3	2
β-strand	188-192	5	1
α-helix	193-202	10
β-strand	205-207	3	1

Chain B: 10 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	5-7	3
α-helix	13-20	8
β-strand	24-29	6	3
α-helix	58-61	4
β-strand	69-72	4	3
α-helix	73-81	9
β-strand	85-88	4	3
α-helix	91-93	3
α-helix	95-107	13
α-helix	119-129	11
α-helix	138-159	22
α-helix	160-162	3
β-strand	168-169	2	4
α-helix	175-199	25

4 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA replication complex GINS protein PSF1	A	protein	208	Saccharomyces cerevisiae W303	Q12488 (AlphaFold model)
DNA replication complex GINS protein PSF2	B	protein	213	Saccharomyces cerevisiae W303	P40359 (AlphaFold model)
DNA replication complex GINS protein PSF3	C	protein	217	Saccharomyces cerevisiae W303	Q12146 (AlphaFold model)
DNA replication complex GINS protein SLD5	D	protein	294	Saccharomyces cerevisiae W303	Q03406 (AlphaFold model)
Cell division control protein 45	E	protein	650	Saccharomyces cerevisiae W303	Q08032
Leading strand DNA template	F	DNA	35	synthetic construct
Lagging strand DNA template	G	DNA	7	synthetic construct
DNA replication licensing factor MCM2	2	protein	868	Saccharomyces cerevisiae W303	P29469
DNA replication licensing factor MCM3	3	protein	971	Saccharomyces cerevisiae W303	P24279
DNA replication licensing factor MCM4	4	protein	933	Saccharomyces cerevisiae W303	P30665
Minichromosome maintenance protein 5	5	protein	775	Saccharomyces cerevisiae W303	P29496
DNA replication licensing factor MCM6	6	protein	1017	Saccharomyces cerevisiae W303	P53091

2 more molecules are not listed.

Sequence of entity 1 (A), FASTA

>9E2Y_1 DNA replication complex GINS protein PSF1 (chains A)
MYGDLGNKLVLEAKRTKQLYARSNQDVNLPMYHEDIIRNILKEVSNLRKNTEYLKEQQQL
GMLDDKVAKCQYFVTLLCMERNKRCLLAYQRLRTDILDSMAWNNNGLDLMSSITFSQQDT
NNLSHQEQEYLKEYCDLITDLKSGDLVDIDLSGSLVPPSDVFIDVRVLKDAGEIQTEYGV
FNLIKDSQFFVRQSDVERLIQQGYLQKI

Sequence of entity 2 (B), FASTA

>9E2Y_2 DNA replication complex GINS protein PSF2 (chains B)
MSLPAHLQQTFSPEEIQFIVENEPIKIFPRITTRQKIRGDDRGTGNHTRWQLITTDDKAL
NNMVAMRSTEVVLWIALLLKQQSKCSIVAPQWLTTKELDRKIQYEKTHPDRFSELPWNWL
VLARILFNKAKDDFHDPIHELRGKIQDLREIRQIKVLKGLKYLNESHLQLDNLSLLEINE
LRPFITEIMDKLREIHTASLTAGTENDEEEFNI

Sequence of entity 3 (C), FASTA

>9E2Y_3 DNA replication complex GINS protein PSF3 (chains C)
MGSSHHHHHHSSGLVPRGSHMASMGYYDIDDVLADGTEFPCKFQYDIPGLGYLENNPGRP
ITKNTKLSLPLWLARILAIVGGDEALVDEEPVPFVELLPPDMFSTKVMNAIKTDPVALDL
HSINSHFFSLAIKWIMLFSEKELANVVSELLLQRAQELNHHASSLSIDLNADSTGKNSAN
TNIATSTFLLKLEEMEKEIYKKSHESYKDTKRWMFKK

Sequence of entity 4 (D), FASTA

>9E2Y_4 DNA replication complex GINS protein SLD5 (chains D)
MDINIDDILAELDKETTAVDSTKITQGSSSTTHRDANTIVGSSLDLNDKTQIYVSPQQDF
SDLMKSWKNERCSPELLPYPHQLMKRLLNRISMQSQLIENISMGFLDMQNASNANPPMPN
ESKLPLLCMETELERLKFVIRSYIRCRLSKIDKFSLYLRQLNEDENSLISLTDLLSKDEI
KYHDTHSLIWLKLVNDSILKYMPEELQAINDTEGSVNMIDEPDWNKFVFIHVNGPPDGKW
NEDPLLQENEFGKPCYTVTIPDLKEEVELTIGSIYVMRYEVIRDLLRDDKVALI

Sequence of entity 5 (E), FASTA

>9E2Y_5 Cell division control protein 45 (chains E)
MYYGISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFKKQLVQSQIVP
IFGYSELRRHYSQLDDNINSLLLVGFGGVIDLEAFLEIDPQEYVIDTDEKSGEQSFRRDI
YVLDAHRPWNLDNIFGSQIIQCFDDGTVDDTLGEQKEAYYKLLELDEESGDDELSGDEND
NNGGDDEATDADEVTDEDEEDEDETISNKRGNSSIGPNDLSKRKQRKKQIHEYEGVLEEY
YSQGTTVVNSISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYPLLQDEVK
RLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSLWNENGKKRLH
KMFARMGIPLSTAQETWLYMDHSIKRELGIIFDKNLDRYGLQDIIRDGFVRTLGYRGSIS
ASEFVEALTALLEVGNSTDKDSVKINNDNNDDTDGEEEEDNSAQKLTNLRKRWVSNFWLS
WDALDDRKVELLNRGIQLAQDLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDGPDLDLYR
NPLTLLRLGNWLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGLDTIHTKKP
ILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL

Sequence of entity 6 (F), FASTA

>9E2Y_6 Leading strand DNA template (chains F)
ATCTGCTTTGGGTGGGTGGGTGGGTTGAGGCAATT

Sequence of entity 7 (G), FASTA

>9E2Y_7 Lagging strand DNA template (chains G)
AGCAGAT

Sequence of entity 8 (2), FASTA

>9E2Y_8 DNA replication licensing factor MCM2 (chains 2)
MSDNRRRRREEDDSDSENELPPSSPQQHFRGGMNPVSSPIGSPDMINPEGDDNEVDDVPD
IDEVEEQMNEVDLMDDNMYEDYAADHNRDRYDPDQVDDREQQELSLSERRRIDAQLNERD
RLLRNVAYIDDEDEEQEGAAQLDEMGLPVQRRRRRRQYEDLENSDDDLLSDMDIDPLREE
LTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIRTLGEMNSE
SLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELHYPDYARIHSEIHVRISDF
PTIYSLRELRESNLSSLVRVTGVVTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIR
ISFCTNCKSKGPFRVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKP
GEEVEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEEE
EREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVL
LLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGALVLAD
KGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNST
LPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENDEDREGEELK
NNGESAIEQGEDEINEQLNARQRRLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQ
MDMDKVSRVYADLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKV
VVDSFVDAQKVSVRRQLRRSFAIYTLGH

Sequence of entity 9 (3), FASTA

>9E2Y_9 DNA replication licensing factor MCM3 (chains 3)
MEGSTGFDGDATTFFAPDAVFGDRVRRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQ
DDADERDLLGDDDGDDLEKEKKAASSTSLNILPHRIIISLDDLREFDRSFWSGILVEPAY
FIPPAEKALTDLADSMDDVPHPNASAVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLV
SVEGIVTKTSLVRPKLIRSVHYAAKTGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNK
LTTEYGYSTFIDHQRITVQEMPEMAPAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKS
LGAGGMNQSNSNTLIGFKTLILGNTVYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIF
DILSQSLAPSIYGHDHIKKAILLMLMGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLR
FVLNTASLAIATTGRGSSGVGLTAAVTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTD
VDRVAIHEVMEQQTVTIAKAGIHTTLNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLL
SRFDLLFVVTDDINEIRDRSISEHVLRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINP
EEHSNSGAGVENEGEDDEDHVFEKFNPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYV
QYAKERVIPQLTQEAINVIVKNYTDLRNDDNTKKSPITARTLETLIRLATAHAKVRLSKT
VNKVDAKVAANLLRFALLGEDIGNDIDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSG
SPIKSTPRRSTASSVNATPSSARRILRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQL
GLRVSPRRREHLHAPEEGSSGPLTEVGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTIST
GRLSLISGIIARLMQTEIFEEESYPVASLFERINEELPEEEKFSAQEYLAGLKIMSDRNN
LMVADDKVWRV

Sequence of entity 10 (4), FASTA

>9E2Y_10 DNA replication licensing factor MCM4 (chains 4)
MSQQSSSPTKEDNNSSSPVVPNPDSVPPQLSSPALFYSSSSSQGDIYGRNNSQNLSQGEG
NIRAAIGSSPLNFPSSSQRQNSDVFQSQGRQGRIRSSASASGRSRYHSDLRSDRALPTSS
SSLGRNGQNRVHMRRNDIHTSDLSSPRRIVDFDTRSGVNTLDTSSSSAPPSEASEPLRII
WGTNVSIQECTTNFRNFLMSFKYKFRKILDEREEFINNTTDEELYYIKQLNEMRELGTSN
LNLDARNLLAYKQTEDLYHQLLNYPQEVISIMDQTIKDCMVSLIVDNNLDYDLDEIETKF
YKVRPYNVGSCKGMRELNPNDIDKLINLKGLVLRSTPVIPDMKVAFFKCNVCDHTMAVEI
DRGVIQEPARCERIDCNEPNSMSLIHNRCSFADKQVIKLQETPDFVPDGQTPHSISLCVY
DELVDSCRAGDRIEVTGTFRSIPIRANSRQRVLKSLYKTYVDVVHVKKVSDKRLDVDTST
IEQELMQNKVDHNEVEEVRQITDQDLAKIREVAAREDLYSLLARSIAPSIYELEDVKKGI
LLQLFGGTNKTFTKGGRYRGDINILLCGDPSTSKSQILQYVHKITPRGVYTSGKGSSAVG
LTAYITRDVDTKQLVLESGALVLSDGGVCCIDEFDKMSDSTRSVLHEVMEQQTISIAKAG
IITTLNARSSILASANPIGSRYNPNLPVTENIDLPPPLLSRFDLVYLVLDKVDEKNDREL
AKHLTNLYLEDKPEHISQDDVLPVEFLTMYISYAKEHIHPIITEAAKTELVRAYVGMRKM
GDDSRSDEKRITATTRQLESMIRLAEAHAKMKLKNVVELEDVQEAVRLIRSAIKDYATDP
KTGKIDMNLVQTGKSVIQRKLQEDLSREIMNVLKDQASDSMSFNELIKQINEHSQDRVES
SDIQEALSRLQQEDKVIVLGEGVRRSVRLNNRV

Sequence of entity 11 (5), FASTA

>9E2Y_11 Minichromosome maintenance protein 5 (chains 5)
MSFDRPEIYSAPVLQGESPNDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYS
LTVNMEHLIGYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRAQSANNNDKDPENT
SMDTDSLLLNSLPTFQLILNSNANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLS
IMCRNCRHTTSITINNFNSITGNTVSLPRSCLSTIESESSMANESNIGDESTKKNCGPDP
YIIIHESSKFIDQQFLKLQEIPELVPVGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSI
YNSKNGAGSGRSGGGNGGSGVAIRTPYIKILGIQSDVETSSIWNSVTMFTEEEEEEFLQL
SRNPKLYEILTNSIAPSIFGNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGT
AKSQLLKFVEKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCID
EFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGRYDDLKSPGDNI
DFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGNANAMQNQQEENGSEISIEKMK
RYITYCRLKCAPRLSPQAAEKLSSNFVTIRKQLLINELESTERSSIPITIRQLEAIIRIT
ESLAKLELSPIAQERHVDEAIRLFQASTMDAASQDPIGGLNQASGTSLSEIRRFEQELKR
RLPIGWSTSYQTLRREFVDTHRFSQLALDKALYALEKHETIQLRHQGQNIYRSGV

Sequence of entity 12 (6), FASTA

>9E2Y_12 DNA replication licensing factor MCM6 (chains 6)
MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLHFPSSSQPHVSNSQTGP
FVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKKVDDVTGEKVREAFEQFLE
DFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSMRENGALAMAISEQYYRFLPF
LQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADEDEQQDDDMNGSSLPRDSGSSAAPGN
GTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRIRDIRSEKIGSLLSISGTVTRTSEV
RPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCPNPSCENRAFWTLNVTRSRFLDWQKVR
IQENANEIPTGSMPRTLDVILRGDSVERAKPGDRCKFTGVEIVVPDVTQLGLPGVKPSST
LDTRGISKTTEGLNSGVTGLRSLGVRDLTYKISFLACHVISIGSNIGASSPDANSNNRET
ELQMAANLQANNVYQDNERDQEVFLNSLSSDEINELKEMVKDEHIYDKLVRSIAPAVFGH
EAVKKGILLQMLGGVHKSTVEGIKLRGDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSG
KASSAAGLTAAVVRDEEGGDYTIEAGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQT
ISIAKAGIHATLNARTSILAAANPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCN
EKIDTELASHIVDLHMKRDEAIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKEL
RKDDAQGFSRSSYRITVRQLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDV
DDVEMDEEFDNIESQSHAASGNNDDNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKK
KTTVTYDKYVSMMNMIVRKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRL
AFKVIKRLVKDRILMEIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS

Ligands and cofactors

ID	Name	Formula	Copies
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	5
ZN	Zinc ion	Zn	5
MG	Magnesium ion	Mg	6
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	1

Primary citation

G-quadruplex-stalled eukaryotic replisome structure reveals helical inchworm DNA translocation. Batra, S., Allwein, B., Kumar, C. et al. Science (2025) 387:eadt1978-eadt1978. DOI 10.1126/science.adt1978 · PubMed

Other PDB entries of the same protein (UniProt Q12488 (AlphaFold model), which also has an AlphaFold model), best resolution first:

28VY 2.7 Å, sCMGE assembled on ARS1 DNA with Sld2 and RPA
8KG6 3.07 Å, Yeast replisome in state I
7PMK 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation I)
7PMN 3.2 Å, S. cerevisiae replisome-SCF(Dia2) complex bound to double-stranded DNA (conformation II)
9RHI 3.2 Å, pre-Initiation Complex on ARS1 DNA (monomer)
9E2W 3.3 Å, Cryo-EM structure of yeast CMG helicase stalled at G4-containing DNA template, state 1
9RHJ 3.4 Å, Pre-Initiation Complex on ARS1 DNA (dimer)
8B9A 3.5 Å, S. cerevisiae replisome + Ctf4, bound by pol alpha primase. Complex engaged with a fork…
8B9B 3.5 Å, S. cerevisiae replisome + Ctf4, bound by pol alpha. Complex engaged with a fork DNA…
9E2X 3.5 Å, Cryo-EM structure of yeast CMG helicase stalled at G4-containing DNA template, state 2
3JC6 3.7 Å, Structure of the eukaryotic replicative CMG helicase and pumpjack motion
6SKL 3.7 Å, Cryo-EM structure of the CMG Fork Protection Complex at a replication fork -…

Browse structure collections

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