7QJE: Gamma-tubulin complex component 4

Structure of recombinant human gamma-Tubulin Ring Complex 4-spoked assembly intermediate (spokes 9-12). Determined by electron microscopy at 7.8 Å resolution. Released 26 Jan 2022.

Method
Electron microscopy
Resolution
7.8 Å
Organism
Homo sapiens
Chains
8
Atoms
31,312
Mol. weight
676.47 kDa
Released
26 Jan 2022

Explore 7QJE in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7QJE contains 160 α-helices and 88 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain I: 21 helices, 4 β-strands

ElementResiduesLengthSheet
α-helix4-96
β-strand1811
β-strand2611
α-helix37-6125
α-helix84-11330
α-helix119-1257
α-helix129-14517
α-helix151-1588
α-helix166-19227
α-helix270-28415
α-helix304-31613
α-helix322-34625
α-helix350-36011
α-helix366-3749
α-helix378-3803
α-helix388-40215
β-strand41312
β-strand45612
α-helix469-49527
α-helix497-5015
α-helix515-5173
α-helix518-55033
α-helix554-57118
α-helix578-59821
α-helix609-62921
Chain J: 28 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix212-2198
α-helix243-25210
α-helix267-27913
β-strand288-28923
β-strand294-29523
α-helix304-3074
α-helix309-33224
α-helix361-38727
α-helix395-4017
α-helix403-41715
α-helix429-44618
α-helix457-46610
α-helix470-48112
α-helix553-5553
α-helix557-57014
α-helix639-6479
α-helix688-71023
α-helix716-72611
α-helix735-7428
α-helix755-7628
α-helix807-8104
α-helix816-83823
α-helix882-89110
α-helix893-9019
α-helix902-9076
α-helix908-91811
α-helix923-94018
α-helix951-96717
α-helix981-99717
α-helix1011-10144
Chain K: 22 helices, 4 β-strands
ElementResiduesLengthSheet
α-helix4-96
β-strand1814
β-strand2614
α-helix37-6125
α-helix84-11330
α-helix119-1257
α-helix129-14517
α-helix151-1588
α-helix166-19227
α-helix270-28718
α-helix304-31613
α-helix322-34625
α-helix350-36011
α-helix366-3749
α-helix378-3803
α-helix388-40215
β-strand41315
β-strand45615
α-helix469-49527
α-helix498-5014
α-helix515-5173
α-helix518-55033
α-helix554-57118
α-helix578-59821
α-helix609-62921
α-helix641-6433
Chain L: 25 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix284-2896
α-helix290-2923
α-helix308-3103
α-helix320-33415
α-helix350-36112
α-helix393-41119
α-helix412-4143
α-helix427-44620
α-helix456-47621
α-helix499-51113
α-helix518-52811
α-helix530-54112
α-helix589-60618
α-helix1482-150322
α-helix1505-151612
α-helix1524-15318
α-helix1550-155910
β-strand1572-157546
β-strand1595-159846
α-helix1602-16043
α-helix1612-164130
α-helix1653-167826
α-helix1679-16835
α-helix1699-171416
α-helix1723-174119
α-helix1762-178827
α-helix1796-18027
Chains W, X, Y and Z: 16 helices, 19 β-strands
ElementResiduesLengthSheet
β-strand4-1077
α-helix12-2817
β-strand3118
β-strand3718
β-strand52-5549
β-strand59-6249
β-strand64-6637
α-helix74-785
β-strand9117
α-helix104-11310
α-helix115-12814
β-strand132-14097
α-helix149-16012
β-strand165-17287
α-helix184-19815
β-strand201-20667
α-helix207-21711
α-helix224-23916
α-helix241-2444
α-helix253-2608
β-strand268-26927
β-strand270-273410
α-helix290-2978
β-strand303110
β-strand317-319311
β-strand320-325610
α-helix330-34213
β-strand348111
β-strand356-361610
α-helix362-3643
β-strand374-379610
β-strand380-381211
α-helix384-40118
α-helix409-4146
α-helix419-43820

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Gamma-tubulin complex component 4I, Kprotein667Homo sapiensQ9UGJ1 (AlphaFold model)
Gamma-tubulin complex component 5Jprotein1024Homo sapiensQ96RT8 (AlphaFold model)
Gamma-tubulin complex component 6Lprotein1819Homo sapiensQ96RT7 (AlphaFold model)
Tubulin gamma-1 chainW, X, Y, Zprotein451Homo sapiensP23258 (AlphaFold model)
Sequence of entity 1 (I, K), FASTA
>7QJE_1 Gamma-tubulin complex component 4 (chains I, K)
MIHELLLALSGYPGSIFTWNKRSGLQVSQDFPFLHPSETSVLNRLCRLGTDYIRFTEFIE
QYTGHVQQQDHHPSQQGQGGLHGIYLRAFCTGLDSVLQPYRQALLDLEQEFLGDPHLSIS
HVNYFLDQFQLLFPSVMVVVEQIKSQKIHGCQILETVYKHSCGGLPPVRSALEKILAVCH
GVMYKQLSAWMLHGLLLDQHEEFFIKQGPSSGNVSAQPEEDEEDLGIGGLTGKQLRELQD
LRLIEEENMLAPSLKQFSLRVEILPSYIPVRVAEKILFVGESVQMFENQNVNLTRKGSIL
KNQEDTFAAELHRLKQQPLFSLVDFEQVVDRIRSTVAEHLWKLMVEESDLLGQLKIIKDF
YLLGRGELFQAFIDTAQHMLKTPPTAVTEHDVNVAFQQSAHKVLLDDDNLLPLLHLTIEY
HGKEHKADATQAREGPSRETSPREAPASGWAALGLSYKVQWPLHILFTPAVLEKYNVVFK
YLLSVRRVQAELQHCWALQMQRKHLKSNQTDAIKWRLRNHMAFLVDNLQYYLQVDVLESQ
FSQLLHQINSTRDFESIRLAHDHFLSNLLAQSFILLKPVFHCLNEILDLCHSFCSLVSQN
LGPLDERGAAQLSILVKGFSRQSSLLFKILSSVRNHQINSDLAQLLLRLDYNKYYTQAGG
TLGSFGM
Sequence of entity 2 (J), FASTA
>7QJE_2 Gamma-tubulin complex component 5 (chains J)
MARHGPPWSRLDAQQERDVRELVRGVAGLQDEADPNFQLALNFAWSNFRFHRFLDVNSHK
IEKTIEGIYEKFVIHSDLSKAASWKRLTEEFLNAPLPSIKEIKTDAHYSILSLLLCLSDS
PSNSSYVETPRNKEVEKKDDFDWGKYLMEDEEMDIGPYMDTPNWSEESEEENDQQPLSRE
DSGIQVDRTPLEEQDQNRKLDPCISWKDEPDDRSWLEHHVVHQYWTARPSQFPHSLHLHS
NLAAVWDQHLYSSDPLYVPDDRVLVTETQVIRETLWLLSGVKKLFIFQLIDGKVTVRNNI
IVTHLTHSCLRSVLEQIAAYGQVVFRLQEFIDEVMGHSSESMLPGSGSVPKKSTEAPFRT
YQAFMWALYKYFISFKEELAEIEKCIINNDTTITLAIVVDKLAPRLSQLKVLHKVFSTGV
AEVPPDTRNVVRASHLLNTLYKAILEYDNVGEASEQTVSLLFSLWVETVRPYLQTVDEWI
VHGHLWDGAREFIIQRNKNVPVNHRDFWYATYTLYSVSEKTENEEKMSDNASASSGSDQG
PSSRQHTMVSFLKPVLKQIIMAGKSMQLLKNLQCAESTTCQAGARDAERKSLYTLFLESV
QSRLRHGEDSTPQVLTEQQATKENLMKMQSIAESHLELDDVHDPLLAINFARMYLEQSDF
HEKFAGGDVCVDRSSESVTCQTFELTLRSCLYPHIDKQYLDCCGNLMQTLKKDYRLVEYL
QAMRNFFLMEGGDTMYDFYTSIFDKIREKETWQNVSFLNVQLQEAVGQRYPEDSSRLSIS
FENVDTAKKKLPVHILDGLTLSYKVPWPVDIVISLECQKIYNQVFLLLLQIKWAKYSLDV
LLFGELVSTAEKPRLKEGLIHEQDTVAQFGPQKEPVRQQIHRMFLLRVKLMHFVNSLHNY
IMTRILHSTGLEFQHQVEEAKDLDQLIKIHYRYLSTIHDRCLLREKVSFVKEAIMKVLNL
ALMFADGWQAGLGTWRMESIEKMESDFKNCHMFLVTILNKAVCRGSFPHLESLALSLMAG
MEQS
Sequence of entity 3 (L), FASTA
>7QJE_3 Gamma-tubulin complex component 6 (chains L)
MASITQLFDDLCEALLPAAKTHLGQRSVNRKRAKRSLKKVAYNALFTNLFQDETQQLQPD
MSKLPARNKILMLSFDLRVGGLGPKADRLEELVEELEAAPCCPLLEVGSVLDLLVQLAGS
GPPQVLPRKRDYFLNNKHVGRNVPYSGYDCDDLSVFEMDVQSLISREECLCHSMIQETLQ
VMEAAPGTGLPTVGLFSFGDPCGDRFERDTRVSLFGALVHSRTYDMDVRLGLPPVPDNAD
LSGLAIKVPPSVDQWEDEGFQSASNLTPDSQSEPSVTPDVDLWEAALTYEASKRRCWERV
GCPPGHREEPYLTEAGRDAFDKFCRLHQGELQLLAGGVLQAPQPVLVKECELVKDVLNVL
IGVVSATFSLCQPAQAFVVKRGVHVSGASPESISSLLSEVAEYGTCYTRLSHFSLQPVLD
SLYSKGLVFQAFTSGLRRYLQYYRACVLSTPPTLSLLTIGFLFKKLGRQLRYLAELCGVG
AVLPGTCGGGPRAAFPTGVKLLSYLYQEALHNCSNEHYPVLLSLLKTSCEPYTRFIHDWV
YSGVFRDAYGEFMIQVNHEYLSFRDKLYWTHGYVLISKEVEDCVPVFLKHIAHDIYVCGK
TINLLKLCCPRHYLCWSDVPVPRISVIFSLEELKEIEKDCAVYVGRMERVARHSSVSKEE
KELRMEIAKQELIAHAREAASRVLSALSDRQMSERMALDARKREQFQRLKEQFVKDQERR
QAARQEELDDDFSYARELRDRERRLKSLEEELERKARQALVDHYSKLSAEAARREQKALW
RIQRHRLESARLRFLLEDEKHIQEMLKAVSEAHQPQEPPDVLLSVHPQVTSPGPEHPEGG
QGCDSGSAEQHSPAWDGWNRPGLLTPQPLKPLAVGAGGRGLQQAEGARPFSDSLSIGDFL
PVGPGAEPSVQTGMVPLLEVALQTINLDLPPSAPGEAPAAASTQPSRPQEYDFSTVLRPA
VATSPAPGPLQAAECSLGSSGLQLWEDSCGKMDACGSASRETLLPSHPPRRAALEEGSSQ
PTERLFGQVSGGGLPTGDYASEIAPTRPRWNTHGHVSDASIRVGENVSDVAPTQPRWNTH
GHVSNASISLGESVSDVAPTRPRWNIHGHVSNASIRVGENVSDVAPTRPRWNTHGHVSNA
SIRVGENVSDVAPTRPRWNTHGHVSDASISLGESVSDMAPARPRWNTHGHVSDASISLGE
SVSDMAPTRPRWNTHGHVSDTSIRVGENVSDVAPIRSRCNTHGHVSDASISLGEPVSDVV
STRPRWNTHVPIPPPHMVLGALSPEAEPNTPRPQQSPPGHTSQSALSLGAQSTVLDCGPR
LPVEVGPSLSSPSSGCGEGSISVGENVSDVAPTQPWWPNTPGDSVSEELGPGRSGDTEDL
SPNWPLNSQEDTAAQSSPGRGEEAEASAAEAQGGEQAYLAGLAGQYHLERYPDSYESMSE
PPIAHLLRPVLPRAFAFPVDPQVQSAADETAVQLSELLTLPVLMKRSITAPLAAHISLVN
KAAVDYFFVELHLEAHYEALRHFLLMEDGEFAQSLSDLLFEKLGAGQTPGELLNPLVLNS
VLSKALQCSLHGDTPHASNLSLALKYLPEVFAPNAPDVLSCLELRYKVDWPLNIVITEGC
VSKYSGVFSFLLQLKLMMWALKDVCFHLKRTALLSHMAGSVQFRQLQLFKHEMQHFVKVI
QGYIANQILHVTWCEFRARLATVGDLEEIQRAHAEYLHKAVFRGLLTEKAAPVMNVIHSI
FSLVLKFRSQLISQAWGPPGGPRGAEHPNFALMQQSYNTFKYYSHFLFKVVTKLVNRGYQ
PHLEDFLLRINFNNYYQDA
Sequence of entity 4 (W, X, Y, Z), FASTA
>7QJE_4 Tubulin gamma-1 chain (chains W, X, Y, Z)
MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHY
IPRAVLLDLEPRVIHSILNSPYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFD
IIDREADGSDSLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSD
VVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSAST
TTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQP
KNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVAL
SRKSPYLPSAHRVSGLMMANHTSISSLFERTCRQYDKLRKREAFLEQFRKEDMFKDNFDE
MDTSREIVQQLIDEYHAATRPDYISWGTQEQ

Primary citation

Modular assembly of the principal microtubule nucleator gamma-TuRC. Wurtz, M., Zupa, E., Atorino, E.S. et al. Nat Commun (2022) 13:473-473. DOI 10.1038/s41467-022-28079-0 · PubMed

Other PDB entries of the same protein (UniProt Q9UGJ1 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 7QJE directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.