7SF7: LPHN3 (ADGRL3) 7TM domain

LPHN3 (ADGRL3) 7TM domain bound to tethered agonist in complex with G protein heterotrimer. Determined by electron microscopy at 2.9 Å resolution. Released 27 Apr 2022.

Method: Electron microscopy
Resolution: 2.9 Å
Organism: Homo sapiens
Chains: 4
Atoms: 6,776
Mol. weight: 106.17 kDa
Released: 27 Apr 2022

Explore 7SF7 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7SF7 contains 29 α-helices and 34 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 15 helices, 0 β-strands

Element	Residues	Length
α-helix	860-878	19
α-helix	881-888	8
α-helix	897-916	20
α-helix	924-956	33
α-helix	961-962	2
α-helix	969-974	6
α-helix	977-988	12
α-helix	1009-1037	29
α-helix	1049-1053	5
α-helix	1054-1057	4
α-helix	1060-1064	5
α-helix	1066-1072	7
α-helix	1081-1089	9
α-helix	1093-1101	9
α-helix	1106-1115	10

Chain B: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	9-12	4
α-helix	13-24	12
α-helix	27-30	4
β-strand	34-39	6	1
α-helix	46-51	6
β-strand	70-75	6	1
β-strand	80-85	6	1
β-strand	105-110	6	1
α-helix	115-117	3
α-helix	118-129	12
β-strand	138-143	6	1
α-helix	158-161	4
α-helix	172-184	13
β-strand	195-199	5	1
α-helix	207-226	20

Chain C: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-12	9
α-helix	22-24	3
α-helix	30-34	5
β-strand	47-51	5	2
β-strand	58-62	5	3
β-strand	71-74	4	3
β-strand	78-81	4	3
β-strand	91-94	4	3
β-strand	100-103	4	4
β-strand	111-116	6	4
β-strand	120-125	6	4
β-strand	134-140	7	4
β-strand	146-153	8	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	175-180	6	5
β-strand	190-192	3	6
β-strand	198-201	4	6
β-strand	207-212	6	6
β-strand	218-223	6	6
β-strand	229-232	4	7
β-strand	240-245	6	7
β-strand	250-254	5	7
β-strand	260-264	5	7
β-strand	273-277	5	8
β-strand	285-289	5	8
β-strand	294-297	4	8
β-strand	304-307	4	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain D: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	10-21	12
α-helix	30-43	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Isoform 1 of Adhesion G protein-coupled receptor L3	A	protein	303	Homo sapiens	Q9HAR2 (AlphaFold model)
G protein subunit 13 (Gi2-mini-G13 chimera)	B	protein	230	Homo sapiens	Q14344 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	D	protein	71	Homo sapiens	P59768 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7SF7_1 Isoform 1 of Adhesion G protein-coupled receptor L3 (chains A)
TNFAVLMAHVEVKHSDAVHDLLLDVITWVGILLSLVCLLICIFTFCFFRGLQSDRNTIHK
NLCISLFVAELLFLIGINRTDQPIACAVFAALLHFFFLAAFTWMFLEGVQLYIMLVEVFE
SEHSRRKYFYLVGYGMPALIVAVSAAVDYRSYGTDKVCWLRLDTYFIWSFIGPATLIIML
NVIFLGIALYKMFHHTAILKPESGCLDNIKSWVIGAIALLCLLGLTWAFGLMYINESTVI
MAYLFTIFNSLQGMFIFIFHCVLQKKVRKEYGKCLRTHCCSGKSTESSIGSGKTSGSLEV
LFQ

Sequence of entity 2 (B), FASTA

>7SF7_2 G protein subunit 13 (Gi2-mini-G13 chimera) (chains B)
MGSTVSAEDKAAAERSKEIDKCLSREKTYVKRLVKILLLGADNSGKSTFLKQMRIIHGGS
GGSGGTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVDSSDFNRLTE
SLNDFETIVNNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPHCLRDVQKFLV
ECFRNKRRDQQQKPLYHHFTTAINTENARLIFRDVKDTILHDNLKQLMLQ

Sequence of entity 3 (C), FASTA

>7SF7_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (D), FASTA

>7SF7_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains D)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Primary citation

The tethered peptide activation mechanism of adhesion GPCRs. Barros-Alvarez, X., Nwokonko, R.M., Vizurraga, A. et al. Nature (2022) 604:757-762. DOI 10.1038/s41586-022-04575-7 · PubMed

Other PDB entries of the same protein (UniProt Q9HAR2 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5AFB 2.16 Å, Crystal structure of the Latrophilin3 Lectin and Olfactomedin Domains
8DJG 2.65 Å, ADGRL3-lectin domain in complex with an activating synthetic antibody fragment
6VHH 2.97 Å, Human Teneurin-2 and human Latrophilin-3 binary complex
8JMT 3.36 Å, Structure of the adhesion GPCR ADGRL3 in the apo state
5CMN 3.6 Å, FLRT3 LRR domain in complex with LPHN3 Olfactomedin domain
8VTI 3.9 Å, Latrophilin-3 (ADGRL3) HormR and GAIN domains in the context of the holoreceptor

Browse structure collections

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