7SIT: Voltage-gated potassium channel subunit beta-2

Crystal structure of Voltage gated potassium ion channel, Kv 1.2 chimera-3m. Determined by X-ray diffraction at 3.32 Å resolution. Released 4 May 2022.

Method

X-ray diffraction

Resolution

3.32 Å

Organism

Rattus norvegicus

Chains

4

Atoms

10,442

Mol. weight

194.01 kDa

Ligands

NAP, O

Released

4 May 2022

Explore 7SIT in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7SIT contains 72 α-helices and 36 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 17 helices, 13 β-strands

Molecules and chains

>7SIT_1 Voltage-gated potassium channel subunit beta-2 (chains A, C)
MLQFYRNLGKSGLRVSCLGLGTWVTFGGQITDEMAEHLMTLAYDNGINLFDTAEVYAAGK
AEVVLGNIIKKKGWRRSSLVITTKIFWGGKAETERGLSRKHIIEGLKASLERLQLEYVDV
VFANRPDPNTPMEETVRAMTHVINQGMAMYWGTSRWSSMEIMEAYSVARQFNLIPPICEQ
AEYHMFQREKVEVQLPELFHKIGVGAMTWSPLACGIVSGKYDSGIPPYSRASLKGYQWLK
DKILSEEGRRQQAKLKELQAIAERLGCTLPQLAIAWCLRNEGVSSVLLGASNAEQLMENI
GAIQVLPKLSSSIVHEIDSILGNKPYSKKDYRS

>7SIT_2 Voltage gated potassium channel Kv1.2-Kv2.1 (chains B, D)
MAHHHHHHHHHHGLVPRGSMTVATGDPVDEAAAHPGHPQDTYDPEADHESSERVVINISG
LRFETQLKTLAQFPETLLGDPKKRMRYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLRRP
VNVPLDIFSEEIRFYELGEEAMEMFREDEGYIKEEERPLPENEFQRQVWLLFEYPESSGP
ARIIAIVSVMVILISIVSFCLETLPIFRDENEDMHGGGVTFHTYSQSTIGYQQSTSFTDP
FFIVETLCIIWFSFEFLVRFFACPSKAGFFTNIMNIIDIVAIIPYYVTIFLTESNKSVLQ
FQNVRRVVQIFRIMRILRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSA
VYFAEADERDSQFPSIPDAFFWAVVTMTTVGYGDMTPTTIGGKIVGSLCAIAGVLTIALP
VPVIVSNFNYFYHRETEGEEQAQYLQVTSSPKIPSSPDLKKSRSASTISKSDYMEIQEGV
NNSNEDFREENLKTANSTLANTNYVNITKMLTDV

Ligands and cofactors

Water and common crystallization additives (K) are not listed.

Primary citation

Structural basis for C-type inactivation in a Shaker family voltage-gated K + channel. Reddi, R., Matulef, K., Riederer, E.A. et al. Sci Adv (2022) 8:eabm8804-eabm8804. DOI 10.1126/sciadv.abm8804 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P62483 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 3EAU 1.82 Å, Voltage-dependent K+ channel beta subunit in complex with cortisone
• 3EB3 2.0 Å, Voltage-dependent K+ channel beta subunit (W121A) in complex with cortisone
• 3EB4 2.0 Å, Voltage-dependent K+ channel beta subunit (I211R) in complex with cortisone
• 1EXB 2.1 Å, Structure of the cytoplasmic beta subunit-T1 assembly of voltage-dependent K channels
• 2R9R 2.4 Å, Shaker family voltage dependent potassium channel (kv1.2-kv2.1 paddle chimera channel)…
• 4JTA 2.5 Å, Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Charybdotoxin
• 4JTD 2.54 Å, Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Lys27Met mutant of…
• 4JTC 2.56 Å, Crystal structure of Kv1.2-2.1 paddle chimera channel in complex with Charybdotoxin in Cs+
• 1QRQ 2.8 Å, Structure of a voltage-dependent K+ channel beta subunit
• 2A79 2.9 Å, Mammalian Shaker Kv1.2 potassium channel- beta subunit complex
• 3LNM 2.9 Å, F233W mutant of the Kv2.1 paddle-Kv1.2 chimera channel
• 3LUT 2.9 Å, A Structural Model for the Full-length Shaker Potassium Channel Kv1.2

Browse structure collections

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