7TYY: Human Amylin2 Receptor

Human Amylin2 Receptor in complex with Gs and salmon calcitonin peptide. Determined by electron microscopy at 3.0 Å resolution. Released 23 Mar 2022.

Method: Electron microscopy
Resolution: 3.0 Å
Organisms: Homo sapiens, Salmo salar, Lama glama
Chains: 7
Atoms: 9,802
Mol. weight: 189.46 kDa
Ligands: PLM, NAG, Y01
Released: 23 Mar 2022

Explore 7TYY in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7TYY contains 50 α-helices and 59 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	13-39	27
β-strand	41-47	7	7
α-helix	53-57	5
β-strand	208-214	7	7
β-strand	217-223	7	7
α-helix	234-238	5
β-strand	243-248	6	7
α-helix	265-277	13
β-strand	286-291	6	7
α-helix	294-302	9
α-helix	313-315	3
α-helix	326-327	2
α-helix	332-349	18
β-strand	359	1	7
β-strand	362-363	2	7
α-helix	371-390	20

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	3-25	23
α-helix	30-33	4
α-helix	38-39	2
β-strand	49-51	3	8
β-strand	58-63	6	9
β-strand	69-74	6	9
β-strand	78-83	6	9
β-strand	89-94	6	9
α-helix	95	1
β-strand	100-105	6	10
β-strand	111-116	6	10
β-strand	121-125	5	10
β-strand	135-139	5	10
β-strand	146-151	6	11
β-strand	156-161	6	11
β-strand	166-170	5	11
β-strand	175-180	6	11
β-strand	187-192	6	12
β-strand	198-203	6	12
β-strand	208-212	5	12
β-strand	218-222	5	12
β-strand	229-234	6	13
β-strand	240-245	6	13
β-strand	250-254	5	13
β-strand	262-264	3	13
β-strand	273-278	6	14
β-strand	284-289	6	14
β-strand	294-298	5	14
β-strand	304-308	5	14
β-strand	317-320	4	8
β-strand	327-330	4	8
β-strand	336-338	3	8

Chain E: 6 helices, 0 β-strands

Element	Residues	Length
α-helix	61-76	16
α-helix	77-82	6
α-helix	90-106	17
α-helix	114-126	13
α-helix	143-148	6
α-helix	151-164	14

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	11-23	13
α-helix	30-43	14
α-helix	53-55	3

Chain N: 2 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	15
β-strand	10-12	3	16
β-strand	17-25	9	15
β-strand	34-39	6	16
β-strand	45-51	7	16
β-strand	58-60	3	16
α-helix	62-64	3
β-strand	68-73	6	15
β-strand	78-84	7	15
β-strand	92-98	7	16
α-helix	99	1
β-strand	118	1	16
β-strand	122-126	5	16

Chain P: 3 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	4	1	1
α-helix	5-7	3
α-helix	8-20	13
α-helix	23-26	4

Chain R: 23 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	39-61	23
β-strand	71-72	2	2
α-helix	73-74	2
β-strand	75-76	2	3
β-strand	81-82	2	3
β-strand	85-86	2	2
α-helix	89-90	2
β-strand	91-94	4	4
α-helix	95-96	2
β-strand	107-110	4	4
β-strand	112	1	5
β-strand	118	1	5
β-strand	120-121	2	6
β-strand	126-127	2	6
β-strand	130	1	4
α-helix	132-135	4
α-helix	138-172	35
α-helix	174-176	3
α-helix	179-200	22
α-helix	201-205	5
α-helix	209-214	6
α-helix	220-248	29
α-helix	260-263	4
α-helix	264-268	5
α-helix	271-283	13
α-helix	288-290	3
α-helix	296-298	3
α-helix	299-328	30
α-helix	334-352	19
α-helix	355-358	4
β-strand	361	1	1
α-helix	366-380	15
α-helix	383-388	6
α-helix	389-393	5
α-helix	396-404	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Receptor activity-modifying protein 2	E	protein	156	Homo sapiens	O60895 (AlphaFold model)
Calcitonin-1	P	protein	33	Salmo salar	B5XGR7 (AlphaFold model)
Calcitonin receptor	R	protein	501	Homo sapiens	P30988 (AlphaFold model)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	A	protein	394	Homo sapiens	P63092 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	350	Homo sapiens	P62873
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Homo sapiens	P59768
nanobody 35	N	protein	138	Lama glama

Sequence of entity 1 (E), FASTA

>7TYY_1 Receptor activity-modifying protein 2 (chains E)
MKTIIALSYIFCLVFADYKDDDDKPLPTTGTPGSEGGTVKNYETAVQFCWNHYKDQMDPI
EKDWCDWAMISRPYSTLRDCLEHFAELFDLGFPNPLAERIIFETHQIHFANCSLVQPTFS
DPPEDVLLAMIIAPICLIPFLITLVVWRSKDSEAQA

Sequence of entity 2 (P), FASTA

>7TYY_2 Calcitonin-1 (chains P)
CSNLSTCVLGKLSQELHKLQTYPRTNTGSGTPX

Sequence of entity 3 (R), FASTA

>7TYY_3 Calcitonin receptor (chains R)
MKTIIALSYIFCLVFADYKDDDDLEVLFQGPAAFSNQTYPTIEPKPFLYVVGRKKMMDAQ
YKCYDRMQQLPAYQGEGPYCNRTWDGWLCWDDTPAGVLSYQFCPDYFPDFDPSEKVTKYC
DEKGVWFKHPENNRTWSNYTMCNAFTPEKLKNAYVLYYLAIVGHSLSIFTLVISLGIFVF
FRSLGCQRVTLHKNMFLTYILNSMIIIIHLVEVVPNGELVRRDPVSCKILHFFHQYMMAC
NYFWMLCEGIYLHTLIVVAVFTEKQRLRWYYLLGWGFPLVPTTIHAITRAVYFNDNCWLS
VETHLLYIIHGPVMAALVVNFFFLLNIVRVLVTKMRETHEAESHMYLKAVKATMILVPLL
GIQFVVFPWRPSNKMLGKIYDYVMHSLIHFQGFFVATIYCFCNNEVQTTVKRQWAQFKIQ
WNQRWGRRPSNRSARAAAAAAEAGDIPIYICHQELRNEPANNQGEESAEIIPLNIIEQES
SAPAGLEVLFQGPHHHHHHHH

Sequence of entity 4 (A), FASTA

>7TYY_4 Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (chains A)
MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKNTIVKQM
RILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELA
NPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLD
KIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFND
VTAIIFVVASSSYNMVIREDNQTNRLQAALKLFDSIWNNKWLRDTSVILFLNKQDLLAEK
VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCY
PHFTCSVDTENIRRVFNDCRDIIQRMHLRQYELL

Sequence of entity 5 (B), FASTA

>7TYY_5 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRT
LRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGN
YVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWD
IETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDIN
AICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYD
DFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 6 (G), FASTA

>7TYY_6 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 7 (N), FASTA

>7TYY_7 nanobody 35 (chains N)
QVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASISY
TGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYRGQ
GTQVTVSSHHHHHHEPEA

Ligands and cofactors

ID	Name	Formula	Copies
PLM	Palmitic acid	C16 H32 O2	5
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	1
Y01	Cholesterol hemisuccinate	C31 H50 O4	2

Primary citation

A structural basis for amylin receptor phenotype. Cao, J., Belousoff, M.J., Liang, Y.L. et al. Science (2022) 375:eabm9609-eabm9609. DOI 10.1126/science.abm9609 · PubMed

Other PDB entries of the same protein (UniProt O60895 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4RWF 1.76 Å, Crystal structure of the CLR:RAMP2 extracellular domain heterodimer with bound…
6V2E 1.83 Å, Crystal structure of the human CLR:RAMP2 extracellular domain heterodimer with bound…
3AQE 2.0 Å, Crystal structure of the extracellular domain of human RAMP2
2XVT 2.05 Å, Structure of the extracellular domain of human RAMP2
7TYX 2.55 Å, Human Amylin2 Receptor in complex with Gs and rat amylin peptide
3AQF 2.6 Å, Crystal structure of the human CRLR/RAMP2 extracellular complex
9BQ3 2.8 Å, Human Amylin2 Receptor in Complex with Gs and Cagrilintide
6UUN 3.0 Å, CryoEM Structure of the active Adrenomedullin 1 receptor G protein complex with…
7TYH 3.3 Å, Human Amylin2 Receptor in complex with Gs and human calcitonin peptide

Browse structure collections

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