7XAT: Somatostatin receptor 2

Structure of somatostatin receptor 2 bound with SST14. Determined by electron microscopy at 2.85 Å resolution. Released 31 Aug 2022.

Method: Electron microscopy
Resolution: 2.85 Å
Organisms: Homo sapiens, synthetic construct, Bos taurus
Chains: 6
Atoms: 8,470
Mol. weight: 184.99 kDa
Released: 31 Aug 2022

Explore 7XAT in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7XAT contains 34 α-helices and 63 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 16 helices, 0 β-strands

Element	Residues	Length
α-helix	42-70	29
α-helix	77-93	17
α-helix	97-104	8
α-helix	114-117	4
α-helix	121-124	4
α-helix	136-144	9
α-helix	158-179	22
α-helix	202-204	3
α-helix	208-211	4
α-helix	218-237	20
α-helix	238-242	5
α-helix	261-269	9
α-helix	272-277	6
α-helix	290-300	11
α-helix	304-307	4
α-helix	317-320	4

Chain B: 10 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	11-26	16
β-strand	34-39	6	1
α-helix	46-51	6
β-strand	185-188	4	1
β-strand	196-200	5	1
α-helix	208-210	3
α-helix	212-215	4
β-strand	220-225	6	1
α-helix	227-229	3
α-helix	232-234	3
α-helix	244-254	11
β-strand	263-268	6	1
α-helix	272-278	7
α-helix	296-308	13
β-strand	319-323	5	1
α-helix	330-350	21

Chain C: 3 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	8-24	17
α-helix	30-33	4
α-helix	38-39	2
β-strand	43	1	2
β-strand	45-52	8	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	89-94	6	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	120-125	6	5
β-strand	134-140	7	5
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	166-170	5	6
β-strand	175-180	6	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	208-212	5	7
β-strand	218-222	5	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	260-264	5	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	303-304	2	9
β-strand	306	1	2
β-strand	308	1	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	335-341	7	3

Chain D: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	7-24	18
α-helix	30-34	5
α-helix	39-43	5
α-helix	53-55	3

Chain E: 1 helix, 26 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	10
β-strand	12	1	11
β-strand	17-25	9	10
α-helix	29-31	3
β-strand	33-39	7	12
β-strand	45-51	7	12
β-strand	58-60	3	12
β-strand	65	1	10
β-strand	68-73	6	10
β-strand	78-84	7	10
β-strand	92-99	8	12
β-strand	111	1	12
β-strand	115-117	3	12
β-strand	119	1	11
β-strand	140-141	2	13
β-strand	146-148	3	14
β-strand	155-161	7	13
β-strand	166	1	15
β-strand	172	1	15
β-strand	174-179	6	14
β-strand	185-190	6	14
β-strand	194-195	2	14
β-strand	203-208	6	13
β-strand	211-216	6	13
β-strand	226-231	6	14
β-strand	239	1	14
β-strand	243-246	4	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Somatostatin receptor type 2,LargeBit	A	protein	563	Homo sapiens, synthetic construct	P30874 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-1	B	protein	354	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	354	Bos taurus	P62871 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	D	protein	71	Bos taurus	P63212 (AlphaFold model)
ScFv16	E	protein	304	Rattus
Somatostatin-14	F	protein	14	Homo sapiens	P61278

Sequence of entity 1 (A), FASTA

>7XAT_1 Somatostatin receptor type 2,LargeBit (chains A)
MKTIIALSYIFCLVFADYKDDDDKGSGSHHHHHHHHHHLEVLFQGPMDMADEPLNGSHTW
LSIPFDLNGSVVSTNTSNQTEPYYDLTSNAVLTFIYFVVCIIGLCGNTLVIYVILRYAKM
KTITNIYILNLAIADELFMLGLPFLAMQVALVHWPFGKAICRVVMTVDGINQFTSIFCLT
VMSIDRYLAVVHPIKSAKWRRPRTAKMITMAVWGVSLLVILPIMIYAGLRSNQWGRSSCT
INWPGESGAWYTGFIIYTFILGFLVPLTIICLCYLFIIIKVKSSGIRVGSSKRKKSEKKV
TRMVSIVVAVFIFCWLPFYIFNVSSVSMAISPTPALKGMFDFVVVLTYANSCANPILYAF
LSDNFKKSFQNVLCLVKVSGTDDGERSDSKQDKSRLNETTETQRTVFTLEDFVGDWEQTA
AYNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIE
EVFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNG
NKIIDERLITPDGSMLFRVTINS

Sequence of entity 2 (B), FASTA

>7XAT_2 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains B)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKNTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 3 (C), FASTA

>7XAT_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSS

Sequence of entity 4 (D), FASTA

>7XAT_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains D)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (E), FASTA

>7XAT_5 ScFv16 (chains E)
MLLVNQSHQGFNKEHTSKMVSAIVLYVLLAAAAHSAFDVQLVESGGGLVQPGGSRKLSCS
ASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQM
TSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSSDIVMTQ
ATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDR
FSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFGAGTKLELVDENLYFQGASHHHH
HHHH

Sequence of entity 6 (F), FASTA

>7XAT_6 Somatostatin-14 (chains F)
AGCKNFFWKTFTSC

Primary citation

Structural insights into the activation of somatostatin receptor 2 by cyclic SST analogues. Bo, Q., Yang, F., Li, Y. et al. Cell Discov (2022) 8:47-47. DOI 10.1038/s41421-022-00405-2 · PubMed

Other PDB entries of the same protein (UniProt P30874 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7T10 2.5 Å, CryoEM structure of somatostatin receptor 2 in complex with somatostatin-14 and Gi3
7XN9 2.6 Å, Crystal structure of SSTR2 and L-054,522 complex
7XNA 2.65 Å, Crystal structure of somatostatin receptor 2 (SSTR2) with peptide antagonist CYN 154806
7T11 2.7 Å, CryoEM structure of somatostatin receptor 2 in complex with Octreotide and Gi3.
7WIG 2.7 Å, Cryo-EM structure of the L-054,264-bound human SSTR2-Gi1 complex
7WIC 2.8 Å, Cryo-EM structure of the SS-14-bound human SSTR2-Gi1 complex
7XAV 2.87 Å, Structure of somatostatin receptor 2 bound with lanreotide.
7XAU 2.97 Å, Structure of somatostatin receptor 2 bound with octreotide.
7UL5 3.1 Å, CryoEM Structure of Inactive SSTR2 bound to Nb6
7XMR 3.1 Å, CryoEM structure of the somatostatin receptor 2 (SSTR2) in complex with Gi1 and its…
7YAC 3.24 Å, Paltusotine-bound SSTR2-Gi complex
7Y24 3.25 Å, Cryo-EM structure of the octreotide-bound SSTR2-miniGo-scFv16 complex

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