8FN1: Go-coupled NTSR1

CryoEM structure of Go-coupled NTSR1. Determined by electron microscopy at 2.88 Å resolution. Released 29 Mar 2023.

Method: Electron microscopy
Resolution: 2.88 Å
Organisms: Rattus norvegicus, Spodoptera frugiperda, Homo sapiens
Chains: 6
Atoms: 8,444
Mol. weight: 148.06 kDa
Released: 29 Mar 2023

Explore 8FN1 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

8FN1 contains 34 α-helices and 62 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 13 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	61-89	29
α-helix	102-123	22
α-helix	124-130	7
α-helix	138-172	35
α-helix	174-180	7
α-helix	183-200	18
α-helix	204-207	4
β-strand	208-212	5	1
β-strand	223-227	5	1
α-helix	231-242	12
α-helix	243-247	5
α-helix	248-266	19
α-helix	303-319	17
α-helix	322-333	12
α-helix	341-370	30

Chain B: 9 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	4-28	25
β-strand	30-37	8	2
α-helix	43-45	3
β-strand	51-52	2	2
β-strand	67-73	7	2
β-strand	76-82	7	2
α-helix	90-92	3
α-helix	94-97	4
β-strand	102-108	7	2
α-helix	118-126	9
α-helix	129-131	3
β-strand	135-141	7	2
α-helix	143-149	7
α-helix	168-180	13
β-strand	190-194	5	2
α-helix	202-222	21

Chain C: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	89-94	6	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	120-125	6	5
α-helix	129-131	3
β-strand	134-140	7	5
β-strand	146-153	8	6
β-strand	156-161	6	6
β-strand	166-170	5	6
β-strand	175-180	6	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	217-222	6	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	259-264	6	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	304-308	5	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain D: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	13-23	11
α-helix	30-43	14
α-helix	45-47	3
α-helix	53-55	3

Chain E: 5 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	10
β-strand	10-12	3	11
β-strand	17-25	9	10
α-helix	29-31	3
β-strand	33-39	7	11
β-strand	45-51	7	11
β-strand	58-60	3	11
β-strand	68-73	6	10
β-strand	78-84	7	10
α-helix	88-90	3
β-strand	92-99	8	11
β-strand	110-111	2	11
β-strand	113	1	10
β-strand	115-119	5	11
α-helix	125-127	3
β-strand	128-130	3	12
β-strand	134-136	3	13
β-strand	143-149	7	12
β-strand	154	1	14
β-strand	160	1	14
β-strand	162-167	6	13
β-strand	174-178	5	13
β-strand	182-183	2	13
α-helix	184	1
β-strand	191-196	6	12
β-strand	199-204	6	12
β-strand	214-219	6	13
α-helix	225	1
β-strand	226-227	2	13
β-strand	231-234	4	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Neurotensin/neuromedin N	F	protein	6	Rattus norvegicus	P20068 (AlphaFold model)
Neurotensin receptor type 1	A	protein	408	Rattus norvegicus	P20789 (AlphaFold model)
Guanine nucleotide-binding protein G(o) subunit alpha	B	protein	228	Spodoptera frugiperda, Homo sapiens	P09471 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	358	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	D	protein	71	Homo sapiens	P59768
scFv16	E	protein	267	Escherichia coli

Sequence of entity 1 (F), FASTA

>8FN1_1 Neurotensin/neuromedin N (chains F)
RRPYIL

Sequence of entity 2 (A), FASTA

>8FN1_2 Neurotensin receptor type 1 (chains A)
MHHHHHHHHHHSDLEVLFQGPLGSGATSESDTAGPNSDLDVNTDIYSKVLVTAIYLALFV
VGTVGNSVTLFTLARKKSLQSLQSTVHYHLGSLALSDLLILLLAMPVELYNFIWVHHPWA
FGDAGCRGYYFLRDACTYATALNVASLSVERYLAICHPFKAKTLMSRSRTKKFISAIWLA
SALLAIPMLFTMGLQNRSADGTHPGGLVCTPIVDTATVKVVIQVNTFMSFLFPMLVISIL
NTVIANKLTVMVHQAAEQGRVCTVGTHNGLEHSTFNMTIEPGRVQALRHGVLVLRAVVIA
FVVCWLPYHVRRLMFCYISDEQWTTFLFDFYHYFYMLTNALFYASSAINPILYNLVSANF
RQVFLSTLACLCPGWRHRRKKRPTFSRKPNSMSSNHAFSTSATRETLY

Sequence of entity 3 (B), FASTA

>8FN1_3 Guanine nucleotide-binding protein G(o) subunit alpha (chains B)
MGCTLSAEDKAAVERSKMIEKNLKEDGISAAKDVKLLLLGADNSGKSTIVKQMKIIHGGS
GGSGGTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVDLSDYNRMHE
SLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYTGPNTYEDAAAYIQA
QFESKNRSPNKEIYCHMTCATDTNNAQVIFDAVTDIIIANNLRGCGLY

Sequence of entity 4 (C), FASTA

>8FN1_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MHHHHHHLEVLFQGPGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR
IQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMT
CAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSG
DTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTF
TGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSG
RLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (D), FASTA

>8FN1_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains D)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 6 (E), FASTA

>8FN1_6 scFv16 (chains E)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAALEVLFQGPHHHHHHHH

Primary citation

Neurotensin Receptor Allosterism Revealed in Complex with a Biased Allosteric Modulator. Krumm, B.E., DiBerto, J.F., Olsen, R.H.J. et al. Biochemistry (2023) 62:1233-1248. DOI 10.1021/acs.biochem.3c00029 · PubMed

Other PDB entries of the same protein (UniProt P20068 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8FMZ 2.59 Å, Neurotensin receptor allosterism revealed in complex with a biased allosteric modulator
4XES 2.6 Å, Structure of active-like neurotensin receptor
4BUO 2.75 Å, High Resolution Structure of Thermostable Agonist-bound Neurotensin Receptor 1 Mutant…
4GRV 2.8 Å, The crystal structure of the neurotensin receptor NTS1 in complex with neurotensin (8-13)
8FN0 2.89 Å, CryoEM structure of Go-coupled NTSR1 with a biased allosteric modulator
4XEE 2.9 Å, Structure of active-like neurotensin receptor
3ZEV 3.0 Å, Structure of Thermostable Agonist-bound Neurotensin Receptor 1 Mutant without Lysozyme…
4BV0 3.1 Å, High Resolution Structure of Evolved Agonist-bound Neurotensin Receptor 1 Mutant without…
5T04 3.3 Å, Structure of constitutively active neurotensin receptor
4BWB 3.57 Å, Structure of Evolved Agonist-bound Neurotensin Receptor 1 Mutant without Lysozyme Fusion
7L0P 4.1 Å, Structure of NTS-NTSR1-Gi complex in lipid nanodisc, canonical state, without AHD
7L0R 4.2 Å, Structure of NTS-NTSR1-Gi complex in lipid nanodisc, noncanonical state, without AHD

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